杨树无性系表型性状及ISSR分子标记遗传多样性分析

【目的】综合表型性状及分子标记多样性分析探明供试的62个杨树无性系的遗传多样性,为杨树进一步遗传改良奠定基础。【方法】选取地径、苗高、叶面积、叶长、叶宽、叶柄长、叶绿素、侧枝数、叶厚、单株总叶片、叶片干质量、叶片含水率等12个表型性状和ISSR分子标记对杨树无性系个体间进行遗传多样性分析,利用PopGen 32、SPSS 16.0及NTsys 2.10e等软件分别计算多样性指数、进行表型性状间的方差分析以及对各无性系进行UPGMA法聚类分析。【结果】侧枝数、单株叶片数、叶片干质量、叶面积以及地径的变异系数均达到了10%以上,叶柄长、叶长、苗高的变异系数也达到了8%以上的水平。利用5条ISSR引物检测到62份杨树无性系多态性谱带百分率平均为89.04%,基因多样度平均为0.4074,Shannon多样性指数(I)平均为0.5304。采用UPGMA法构建的形态和分子标记聚类图将供试材料聚成的类群均有家系内聚为一类的趋势,但也存在较大的差异;表型性状聚类分析主要是根据叶片的相关性状相似度越高的被聚为一个类群;分子标记聚类主要呈现出亲缘关系越近的无性系越容易聚为一个类群的聚类规律。【结论】供试的杨树无性系间表型性状分化程度高,具有丰富的遗传多样性,研究结果为杨树种质资源的改良、种质创新及多元化开发利用提供科学依据。

基于ISSR-PCR体系鉴别樟芝单核体交配型

通过原生质体单核化技术获得樟芝单核体,基于内转录间隔区(internal transcribed spacer,ITS)序列进行鉴定,采用14个引物对简单重复序列区间(inter-simple sequence repeats,ISSR)进行多态性扩增,筛选条带清晰、重复性好的引物用于樟芝单核体的交配型鉴定。结果表明,通过原生质体单核化技术获得31个樟芝单核体,经ITS序列分析确定获得的单核体为樟芝。以单核体S2、S10、S14、S27的DNA为模板,对14条ISSR引物进行初步筛选,得到4个条带清晰、重复性好的引物P7、P9、P21、P25用于交配型鉴定。单核体S9和S25为同一交配型,两者与S2为不同交配型,通过镜检进一步验证S9、S25可以与S2形成具有锁状联合的双核菌株。该方法可明显缩短樟芝单核体交配型的鉴定时间。

应用拮抗和ISSR标记鉴定64个金针菇菌株的亲缘关系

以16个野生金针菇(Flammulina velutipes)菌株和48个栽培金针菇菌株为材料,评价不同菌株间体细胞不亲和性,并利用ISSR分子标记检测,从细胞学水平和分子水平对金针菇种质资源进行鉴定和遗传多样性分析,明确其亲缘关系。结果表明,64个金针菇菌株基于拮抗试验划分为7个类群,基于ISSR聚类分析划分为5个类群;栽培金针菇菌株之间亲缘关系普遍较近,野生金针菇菌株与栽培金针菇菌株大多具有较远的亲缘性;32号栽培菌株和49号野生菌株可以优先选作金针菇杂交育种亲本。

基于ISSR和TRAP分子标记的东北地区栽培黑木耳遗传多样性研究

为探究东北地区黑木耳栽培菌株的遗传多样性及亲缘关系,采用ISSR和TRAP分子标记对50株栽培黑木耳菌株进行遗传多样性分析。利用22条高多态性、高分辨率的ISSR和TRAP分子标记引物,对栽培黑木耳进行了DNA扩增。结果表明,黑木耳ISSR和TRAP标记谱带多态性较高,分别占89.58%和84.71%,遗传相似系数为0.53~1.00;在ISSR标记的聚类图中相似系数大于0.96的菌株占44.0%,TRAP标记的聚类图中相似系数大于0.96的菌株占54.0%;STRUCTURE聚类结果显示,50株菌株可分为四大类;遗传多样性分析结果表明,东北地区黑木耳栽培菌株的遗传多样性主要来源于群体间,黑木耳栽培菌株的遗传多样性欠丰富,生产菌种资源的同质化较为突出。

基于ISSR标记的航天香蕉新材料的遗传特异性

随着航天事业的发展,将传统的育种技术与航天诱变技术相结合实现育种目标的方法已经得到育种专家的广泛认可。福建香蕉产业受到病害、寒害和风害的威胁,以及外来或进口香蕉的冲击,种植规模正面临着萎缩的处境,使香蕉新品种的选育成为香蕉产业研究亟需解决的重要课题。本研究前期以巴西蕉为母本,在航天空间诱变的基础上,经过连续栽培、筛选和观察,选育出生物学和形态学特征差异明显的2份材料。为鉴定自主选育的以上2份航天香蕉材料的遗传特异性,本研究采用ISSR分子标记技术,从30条引物中筛选出2条多态性好的引物,对2份航天香蕉种质和18份现有香蕉种质进行分析。结果表明:筛选的2条引物共扩增出17条清晰的DNA条带,其中多态性条带13条,占总条带数的76.47%,在DNA分子水平上显示出较高的多态位点和丰富的遗传多样性。凝胶电泳结果显示,结合ISSR引物UBC881和UBC847,2份航天香蕉材料均存在特异性扩增条带,可区别于其他18份香蕉种质。聚类分析结果显示,20份香蕉种质材料间的遗传相似性系数介于0.545~1.000之间,平均值为0.905;在遗传相似性系数为0.960时,所选育的2份航天香蕉材料之间,2份航天香蕉材料与母本之间,以及2份航天香蕉材料与其他17份香蕉种质之间均有明显的遗传差异。本研究结果为航天香蕉新品种的选育和鉴定提供依据。

基于形态标记和ISSR分子标记的长瓣铁线莲遗传多样性分析

为快速区分鉴定市场上繁多的长瓣铁线莲(Clematis macropetala)品种,了解长瓣铁线莲间的遗传多样性水平及亲缘关系,本试验利用形态标记结合ISSR分子标记对25个长瓣铁线莲品种和1个野生种进行遗传多样性研究。结果表明:14个数量形状的变异系数在0.24%~2.32%之间;15个假质量性状的信息多样性指数H在0~2.29%之间,遗传多样性指数D在0~0.94%之间;在欧式距离为15时,可将29个表型性状划分为七类,在欧式距离为15时,可将26个长瓣铁线莲划分为五类。12条引物共扩增出144个条带,多态性条带占比为100%。利用引物UBC815、UBC824和UBC836构建26个长瓣铁线莲的指纹图谱,可快速区分鉴定26个长瓣铁线莲。UPGMA聚类结果显示,在遗传相似系数为0.68时,可将26个长瓣铁线莲划分为八大类。形态标记结合ISSR分子标记可有效鉴别长瓣铁线莲种质资源,以期为长瓣铁线莲种质资源收集保存、创制新品种等提供理论基础。

Genetic Diversity of Jute Mallow (Corchorus spp.) Accessions Based on ISSR Markers

Jute mallow is a nutritious leafy vegetable. The leaves are rich in proteins, vitamins and essential amino acids. Molecular characterization of Jute mallow with focus on improvement of leaf yield is scarcely reported. In the present study, inter sequence simple repeats (ISSR) molecular markers were employed to assess genetic diversity and relationships of 83 accessions of Jute mallow from different parts of Africa and Asia conserved at the World Vegetable Center East and Southern Africa. A total of 89 bands were amplified by 8 ISSR primers. Number of polymorphic bands per primer ranged from 2 to 6 with an average of 2.75 bands per primer. Polymorphic information content (PIC) values ranged from 0.390 to 0.760 with average of 0.53. Average Nei’s gene diversity (h) and Shannon’s information index (I) were 0.335 and 0.494 respectively. The highest pairwise genetic distance was 0.431 observed in a population from East Africa accessions. PC1 and PC2 axis explained 21.69% and 11.66% of the total variation respectively. UPGMA cluster analysis grouped the accessions into six main clusters at genetic similarity coefficient of 0.53 as standard value for classification. These results have important implications for jute mallow breeding and conservation.

鸡血藤ISSR体系优化及引物筛选

以鸡血藤的入药部位藤茎为试验材料,采用改良CTAB法进行DNA提取,利用正交试验和单因素试验对ISSR-PCR反应体系进行优化和构建。结果表明,鸡血藤最佳ISSR-PCR反应体系为DNA模板60 ng,Taq酶2.00 U,Mg2+浓度1.75 mmol/L,dNTP浓度0.25 mmol/L,引物浓度0.20 μmol/L,最佳退火温度44.6℃。从100条ISSR引物筛选出20条适合鸡血藤的ISSR引物。

Comparative analyses of mitogenomes in the social bees with insights into evolution of long inverted repeats in the Meliponini

The insect mitogenome is typically a compact circular molecule with highly conserved gene contents.Nonetheless,mitogenome structural variations have been reported in specific taxa,and gene rearrangements,usually the tRNAs,occur in different lineages.Because synapomorphies of mitogenome organizations can provide information for phylogenetic inferences,comparative analyses of mitogenomes have been given increasing attention.However,most studies use a very few species to represent the whole genus,tribe,family,or even order,overlooking potential variations at lower taxonomic levels,which might lead to some incorrect inferences.To provide new insights into mitogenome organizations and their implications for phylogenetic inference,this study conducted comparative analyses for mitogenomes of three social bee tribes(Meliponini,Bombini,and Apini)based on the phylogenetic framework with denser taxonomic sampling at the species and population levels.Comparative analyses revealed that mitogenomes of Apini and Bombini are the typical type,while those of Meliponini show diverse variations in mitogenome sizes and organizations.Large inverted repeats(IRs)cause significant gene rearrangements of protein coding genes(PCGs)and rRNAs in Indo-Malay/Australian stingless bee species.Molecular evolution analyses showed that the lineage with IRs have lower dN/dS ratios for PCGs than lineages without IRs,indicating potential effects of IRs on the evolution of mitochondrial genes.The finding of IRs and different patterns of gene rearrangements suggested that Meliponini is a hotspot in mitogenome evolution.Unlike conserved PCGs and rRNAs whose rearrangements were found only in the mentioned lineages within Meliponini,tRNA rearrangements are common across all three tribes of social bees,and are significant even at the species level,indicating that comprehensive sampling is needed to fully understand the patterns of tRNA rearrangements,and their implications for phylogenetic inference.

Repeatability,interocular correlation and agreement of optic nerve head vessel density in healthy eyes:a sweptsource optical coherence tomographic angiography study

AIM:To assess the repeatability,interocular correlation,and agreement of quantitative swept-source optical coherence tomography angiography(OCTA)optic nerve head(ONH)parameters in healthy subjects.METHODS:Thir ty-three healthy subjects were enrolled.The ONH of both eyes were imaged four times by a swept-source-OCTA using a 3 mm×3 mm scanning protocol.Images of the radial peripapillary capillary were analyzed by a customized Matlab program,and the vessel density,fractal dimension,and vessel diameter index were measured.The repeatability of the four scans was determined by the intraclass correlation coefficient(ICC).The most well-centered optic disc from the four repeated scans was then selected for the interocular correlation and agreement analysis using the Pearson correlation coefficient,ICC and Bland-Altman plots.RESULTS:All swept-source-OCTA ONH parameters exhibited certain repeatability,with ICC>0.760 and coefficient of variation(CoV)≤7.301%.The obvious interocular correlation was observed for papillary vessel density(ICC=0.857),vessel diameter index(ICC=0.857)and fractal dimension(ICC=0.906),while circumpapillary vessel density exhibited moderate interocular correlation(ICC=0.687).Bland-Altman plots revealed an agreement range of-5.26%to 6.21%for circumpapillary vessel density.CONCLUSION:OCTA ONH parameters demonstrate good repeatability in healthy subjects.The interocular correlations of papillary vessel density,fractal dimension and vessel diameter index are high,but the correlation for circumpapillary vessel density is moderate.

Changes in macrophage infiltration and podocyte injury in lupus nephritis patients with repeated renal biopsy: Report of three cases

BACKGROUND In this study,we retrospectively analysed macrophage infiltration and podocyte injury in three patients with diffuse proliferative lupus nephritis(LN)who un-derwent repeated renal biopsy.CASE SUMMARY Clinical data of three diffuse proliferative LN patients with different pathological characteristics(case 1 was LN IV-G(A),case 2 was LN IV-G(A)+V,and case 3 was LN IV-G(A)+thrombotic microangiopathy)were reviewed.All patients underwent repeated renal biopsies 6 mo later,and renal biopsy specimens were studied.Macrophage infiltration was assessed by CD68 expression detected by immunohistochemical staining,and an immunofluorescence assay was used to detect podocin expression to assess podocyte damage.After treatment,Case 1 changed to LN III-(A),Case 2 remained as type V LN lesions,and Case 3,which changed to LN IV-S(A),had the worst prognosis.We observed reduced macro-phage infiltration after therapy.However,two of the patients with active lesions after treatment still showed macrophage infiltration in the renal interstitium.Before treatment,the three patients showed discontinuous expression of podocin.Notably,the integrity of podocin was restored after treatment in Case 1.CONCLUSION It may be possible to reverse podocyte damage and decrease the infiltrating ma-crophages in LN patients through effective treatment.

Toward understanding the role of genomic repeat elements in neurodegenerative diseases

Neurodegenerative diseases cause great medical and economic burdens for both patients and society;however, the complex molecular mechanisms thereof are not yet well understood. With the development of high-coverage sequencing technology, researchers have started to notice that genomic repeat regions, previously neglected in search of disease culprits, are active contributors to multiple neurodegenerative diseases. In this review, we describe the association between repeat element variants and multiple degenerative diseases through genome-wide association studies and targeted sequencing. We discuss the identification of disease-relevant repeat element variants, further powered by the advancement of long-read sequencing technologies and their related tools, and summarize recent findings in the molecular mechanisms of repeat element variants in brain degeneration, such as those causing transcriptional silencing or RNA-mediated gain of toxic function. Furthermore, we describe how in silico predictions using innovative computational models, such as deep learning language models, could enhance and accelerate our understanding of the functional impact of repeat element variants. Finally, we discuss future directions to advance current findings for a better understanding of neurodegenerative diseases and the clinical applications of genomic repeat elements.

GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.

蒜头果ISSR-PCR反应体系建立及验证

【目的】建立稳定的蒜头果ISSR-PCR反应体系,筛选出适用于蒜头果的多态性引物,为后续蒜头果遗传多样性研究及种质资源保护提供参考依据。【方法】采用单因素试验与L25(54)正交试验相结合的方法,对影响ISSR-PCR反应体系扩增效果的4个主要因素(模板DNA用量、引物浓度、d NTPs浓度、Taq DNA聚合酶)及反应循环数进行优化,确定最佳反应体系和条件。在此基础上筛选出适用于蒜头果的多态性引物,探究其最佳退火温度,并采集云南省境内3个野生居群10个蒜头果样品对优化的反应体系进行验证。【结果】蒜头果ISSR-PCR最佳反应体系(20.0μL):模板DNA 30 ng,ISSR引物0.3μmol/L,d NTPs 0.250 mmol/L,Taq DNA聚合酶1.00 U,dd H2O补足至20.0μL。ISSR最佳反应程序为30个循环。利用优化后的反应体系筛选出了8条引物(UBC825、UBC826、UBC827、UBC836、UBC844、UBC861、UBC834和UBC851)的最佳退火温度,分别为50.2、56.5、50.2、56.5、50.2、50.2、50.2和54.0℃,部分引物(如UBC827、UBC836、UBC861等)实测最佳退火温度与软件预测退火温度存在明显差异。采用优化后的ISSR-PCR反应体系及引物对10个蒜头果样品进行ISSR-PCR分子标记检测,结果显示建立的ISSR-PCR反应体系稳定可靠,不同采样点的蒜头果在遗传上相对保守,且10个蒜头果样品的遗传分化系数(Gst)为0.74,说明其74%的遗传多样性存在于居群间,且基因流(N_(m))为0.18,远小于1.00,说明发生遗传漂变的概率大,易发生遗传多样性降低及居群分化。【结论】通过优化蒜头果ISSR-PCR反应体系,建立可用于蒜头果ISSR-PCR分子标记扩增的稳定反应体系,可用于蒜头果种质资源保护与利用研究工作。

基于ISSR标记分析连云港地区野生灵芝种质资源遗传多样性及亲缘关系

为分析连云港地区野生灵芝种质资源之间的亲缘关系和多样性水平,以连云港地区15株野生灵芝为研究对象,采用ISSR分子标记进行遗传多样性及亲缘关系分析。15株野生灵芝中扩增出多态性条带为40条,片段大小在250~2000 bp之间,平均等位基因位点1.8个,平均有效等位基因数1.2454,平均Nei's遗传多样性0.1742,平均Shannon信息指数0.2925,有效等位基因数(Ne)的变化范围为1.0000~1.6423,Nei's遗传多样性(He)的变化范围为0.1031~0.3911,Shannon信息指数(I)的变化范围为0.2235~0.5799,根据ISSR分子标记的遗传聚类图,15株灵芝相似系数范围在0.415~0.866之间,相似系数为0.63时,可以将15株野生灵芝分为四组。研究结果为连云港地区灵芝种质资源分类、保护和品种创制提供了参考依据。

DNA Tandem Repeats as Iterable Objects to Count Cell Divisions: A Computational Model

Cell lineages of nematodes are completely known: the adult male of Caenorhabditis elegans contains 1031 somatic cells, the hermaphrodite 959, not one more, not one less;cell divisions are strictly deterministic (as in the great majority of invertebrates) but so far nothing is known about the mechanism used by cells to count precise numbers of divisions. In vertebrates, each species has its invariable deterministic numbers of somites, vertebrae, fingers, and teeth: counting the number of iterations is a widespread process in living beings;nonetheless, it remains an unanswered question and a great challenge in cell biology. This paper introduces a computational model to investigate the possible role of satellite DNA in counting cell divisions, showing how cells may operate under Boolean logic algebra. Satellite DNA, made up of repeated monomers and subject to high epigenetic methylation rates, is very similar to iterable sequences used in programming: just like in the “iteration protocol” of algorithms, the epigenetic machinery may run over linear tandem repeats (that hold cell-fate data), read and orderly mark one monomer per cell-cycle (cytosine methylation), keep track and transmit marks to descendant cells, sending information to cell-cycle regulators.

基于ISSR分子标记的44种山药种质资源遗传多样性分析

为探讨不同地区山药种质资源类型及遗传多样性亲缘关系,为山药品种选育、种质资源的保护利用提供参考。采用ISSR标记技术对44份山药种质资源进行遗传多样性及聚类分析。结果表明:从100条随机引物中筛选出8条随机引物,8条引物扩增获得的总谱带数为87条,多态性谱带数为87条,多态性谱带比例为100%。聚类分析将44份种质资源分成4个类群,从结果看ISSR标记技术聚类分析与植物分类学进行的基原分类结果基本一致,44份山药种质资源的遗传距离在0.0313~0.7216,以遗传距离为0.5618进行划分,可以将44份山药种质资源划分为5类,其中薯蓣与褐苞薯蓣亲缘关系最近,与山薯较远,与参薯距离最远。

番石榴遗传多样性的ISSR分析及指纹图谱构建

为研究59份番石榴种质资源的遗传多样性,并绘制指纹图谱。以番石榴为试材,利用ISSR分子标记技术和UPGMA聚类分析方法,绘制番石榴DNA指纹图谱。利用筛选获得的10条ISSR核心引物对59份番石榴样品进行扩增,共得到126条条带,其中多态性条带113条,多态率为89.68%。通过UPGMA聚类分析得知,59份供试番石榴种质资源样品间遗传相似系数为0.50~0.96,平均遗传相似系数为0.73。根据遗传距离的远近,可将59份种质资源分成4组,第1组包含种质34份,第2组包含种质6份,第3组包含种质18份,编号C26‘草莓’番石榴样品单独为第4组。成功构建了59份番石榴种质的DNA指纹图谱,可用于番石榴种质资源的分类与鉴定。

不同来源葡萄种质资源果实叶酸含量及ISSR比较分析

【目的】比较不同来源葡萄果实中叶酸积累的差异,并利用分子标记技术对高叶酸种质资源进行评价和筛选。【方法】采用ELISA技术检测22份葡萄种质资源果实叶酸含量;利用ISSR分子标记对22份葡萄种质资源进行基因组多态性分析和聚类分析;分析检测出叶酸含量与聚类结果。【结果】22份葡萄种质资源果实叶酸含量的范围在1.93~46.95 ng/g,在遗传相似系数为0.621处,22份葡萄材料明显分为2大类群。第1大类叶酸含量平均为35.79 ng/g;其余为第2大类,叶酸含量平均为15.71 ng/g。在遗传相似系数为0.622处,21份葡萄材料又明显分为2大类群。第1大类叶酸含量平均为16.53 ng/g;第2大类叶酸含量平均为12.24 ng/g。欧亚种的叶酸含量较欧美杂种稍高3.9987 ng/g;从30条引物中筛选出9条扩增稳定且多态性丰富的引物用于葡萄的ISSR扩增。共扩增出78条条带,其中多态性条带70条,多态性百分率为89.7%。22份葡萄种质资源的遗传相似系数为0.4744~0.8462,平均遗传相似系数为0.6545。在遗传相似系数为0.621处,22份葡萄材料明显分为2大类群。第1大类包含1个欧亚种(红巴拉多),其余为第2大类。在遗传相似系数为0.622处,21份葡萄材料又明显分为2大类群。第1大类包含11个欧亚种(炎兴无核、灰比诺、瑞都翠霞、京蜜、黑奇无核、无核白、1124、京翠、红宝石、无核紫、达米娜),6个欧美杂种(杂1、夏黑、蜜红、红标无核、洛浦早生、宇选1号)。第2大类包含4个欧亚种(美人指、粉红亚都蜜、木纳格、里扎马特)。【结论】群体1的叶酸含量比群体2的叶酸含量高,欧亚种的叶酸含量较欧美杂种稍高。

基于ISSR分子标记的中华鳖种群遗传多样性分析

利用简单重复序列间扩增(inter simple sequence repeat, ISSR)分子标记技术对5种中华鳖(Pelodiscus sinensis)种群的遗传多样性以及亲缘关系进行分析。结果显示:采用筛选出的10条ISSR引物对来自5个种群的中华鳖个体样本的基因组DNA进行PCR扩增,共获得123条清晰的扩增位点,平均多态性条带(PPB)比例为73.417%。5个居群的等位基因数(Na)、有效等位基因数(Ne)、Nei’s基因多样性指数(H)、Shannon多样性指数(I)分别为1.748 0、1.417 7、0.245 5、0.370 4,其中乌鳖群体的遗传多样性水平高于其他中华鳖,淮河鳖群体的变异程度最小。5个居群总遗传多样性指数(Ht)=0.240 0±0.037 1,居群内遗传多样性指数(Hs)=0.174 7±0.021 6,基因分化系数(Gst)=0.271 9,基因流(Nm)=1.339 0。总遗传变异结果显示:有27.19%的变异发生在居群间,72.81%的变异发生在居群内。杂交鳖与日本品系之间遗传一致度最高(0.937 1),遗传距离最小(0.064 9);中国台湾品系和乌鳖的遗传距离最大(0.127 5)。聚类分析表明,杂交鳖、日本品系和淮河品系聚为一类群,乌鳖与其他品系差异较大,单独为一类群。该研究结果丰富了中华鳖分子标记信息,分析当前中华鳖遗传资源形势,为新品种选育改良、种质资源管理等提供参考资料和理论指导。