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Sex-specific facilitation and reproduction of the gynodioecious cushion plant Arenaria polytrichoides on the Himalaya-Hengduan mountains,SW China 被引量:1
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作者 Xufang Chen Yazhou Zhang +3 位作者 Lishen Qian Renyu Zhou Hang Sun Jianguo Chen 《Plant Diversity》 SCIE CAS CSCD 2024年第2期247-255,共9页
When benefiting other beneficiaries,cushion plants may reciprocally receive feedback effects.The feedback effects on different sex morphs,however,remains unclear.In this study,taking the gynodioecious Arenaria polytri... When benefiting other beneficiaries,cushion plants may reciprocally receive feedback effects.The feedback effects on different sex morphs,however,remains unclear.In this study,taking the gynodioecious Arenaria polytrichiodes as a model species,we aimed to assess the sex-specific facilitation intensity of cushion plant by measuring the beneficiary cover ratio,and to assess the potential costs in cushion reproductive functions by measuring the flower and fruit cover ratios.The total beneficiary cover ratio was similar between females and hermaphrodites.Females produced much less flowers but more fruits than hermaphrodites.These results suggested that females and hermaphrodites possess similar facilitation intensity,and female cushion A.polytrichoides may allocate more resources saved from pollen production to seed production,while hermaphrodites possibly allocate more resources to pollen production hence reducing seed production.The surface areas covered by beneficiaries produced less flowers and fruits than areas without beneficiaries.In addition,strong negative correlations between beneficiary cover and flower cover were detected for both females and hermaphrodites,but the correlation strength were similar for these two sex morphs.However,the correlation between beneficiary cover and fruit cover was only significantly negative for females,suggesting that beneficiary plants negatively affect fruit reproduction of females while have neutral effects on hermaphrodites.All the results suggest that to facilitate other beneficiaries can induce reproductive costs on cushion A.polytrichoides,with females possibly suffering greater cost than hermaphrodites.Such differentiation in reproductive costs between sex morphs,in long-term perspective,may imply sex imbalance in population dynamics. 展开更多
关键词 Facilitation intensity Feedback effect Sex-specific facilitation Nurse plant Population dynamics Reproductive function
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Asexual Reproduction of Aurelia coerulea Polyps in situ on Settling Plates in Jiaozhou Bay,China
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作者 MI Tiezhu ZHU Jianbin +2 位作者 ZHEN Yu WANG Guoshan WANG Jianyan 《Journal of Ocean University of China》 SCIE CAS CSCD 2024年第5期1371-1380,共10页
Jellyfish blooms are increasingly prevalent coastal phenomena,with Aurelia coerulea being a significant contributor.The complex life history of jellyfish,featuring a sessile polyp stage,is a key factor in bloom format... Jellyfish blooms are increasingly prevalent coastal phenomena,with Aurelia coerulea being a significant contributor.The complex life history of jellyfish,featuring a sessile polyp stage,is a key factor in bloom formation,yet it is challenging to study in the field.This study aimed to describe the asexual reproduction of A.coerulea polyps in Jiaozhou Bay,China,and to assess their ability to form sedentary colonies and their relationship with environmental factors.Using settling plates,we observed polyps in situ and documented three asexual reproduction modes:budding,strobilation,and podocyst formation.Environmental parameters including temperature,salinity,dissolved oxygen(DO),chlorophyll a(Chl a),and the biomass of phytoplankton and zooplankton were monitored.Budding was the predominant reproductive method,featuring stolon budding in autumn and direct budding during the winter and spring seasons.Strobilation took place in the colder months,releasing ephyrae between April and May.Polyp abundance was positively correlated with seawater temperature and negatively correlated with DO concentration.Polyps at 1.0 m depth showed higher survivorship and proliferation over 8 months,whereas those at 2.0 m depth experienced a steady decline and died within 4 months.The study enhances our understanding of the life cycle and asexual reproduction strategies of A.coerulea polyps in the field,with implications for predicting and managing jellyfish blooms.The findings underscore the importance of temperature and DO in polyp survival and suggest that initial polyp population proliferation is crucial for long-term survival. 展开更多
关键词 jellyfish bloom POLYPS BUDDING strobilation asexual reproduction
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The life-history trait trade-offs mediated by reproduction and immunity in the brown planthopper,Nilaparvata lugens Stål
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作者 Dan Sun Hongfeng Wang +4 位作者 Jiahui Zeng Qiuchen Xu Mingyun Wang Xiaoping Yu Xuping Shentu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第6期2018-2032,共15页
Reproduction and immune defense are costly functions,and they are expected to tradeoff with each other to drive evolution.The brown planthopper(BPH),Nilaparvata lugens Stål(Hemiptera,Delphacidae),is a global supe... Reproduction and immune defense are costly functions,and they are expected to tradeoff with each other to drive evolution.The brown planthopper(BPH),Nilaparvata lugens Stål(Hemiptera,Delphacidae),is a global superpest that mostly damages rice crops.Yeast-like symbionts(YLS)exist in the abdominal fat body tissue and are tightly associated with the development,growth,and reproduction of BPH.Our previous research demonstrated that mating behavior promotes the release of YLS from the fat body into the hemolymph in the BPH,thereby triggering an immune response.Additionally,the fitness costs related to life-history traits of BPH(such as survival rate)have a strong dependence on the relative abundance of YLS.However,the possible relationship between reproduction and the immune response in BPH has not been identified.In this study,an omics-based approach was used to analyze the transcriptome of fat body tissues in mated and unmated BPH at 72 h post-eclosion,from which two antimicrobial peptide genes,NlDefensin A(NlDfA)and NlDefensin B(NlDfB),were selected since they were highly expressed in mated BPH.Subsequently,the full-length cDNA sequences of the NlDfA and NlDfB genes were cloned and analyzed.qPCR results showed up-regulation of the NlDfA and NlDfB genes in mated BPH when compared to unmated BPH.Spatial-temporal expression analysis indicated that the NlDfA and NlDfB genes were expressed in all tissues and developmental stages,and they were most highly expressed in the fat body at 24 h post-eclosion.Moreover,the symbionts in BPH were significantly inhibited by the in vitro expression of the NlDfA and NlDfB proteins.Furthermore,RNA interference(RNAi)-mediated suppression of NlDfA and NlDfB dramatically increased the relative abundance of YLS in the fat body,while YLS in the hemolymph decreased significantly.These BPHs also displayed some fitness disadvantages in survival,fecundity,hatchability,and possibly the vertical transmission of YLS from hemolymph to egg.Our results indicated that mating could heighten the immunity of BPH by upregulating the expression of the NlDfA and NlDfB genes,which protect the host from pathogen challenges during reproduction.However,the reduced content of YLS may act as a fitness disadvantage in dictating the life-history traits of BPH.This work has significant theoretical and practical implications for the precise green control technology that involves crucial gene targeting,as well as for the“endosymbionts for pest control”strategy in insects. 展开更多
关键词 Nilaparvata lugens reproduction immune defense trade-off
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Tissue distribution of cadmium and its effect on reproduction in Spodoptera exigua
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作者 Honghua Su Menglu Wu +3 位作者 Yong Yang Yan Deng Yizhong Yang Qingming Sun 《Journal of Integrative Agriculture》 SCIE CSCD 2024年第1期195-204,共10页
Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua... Vegetable fields are often contaminated by heavy metals,and Spodoptera exigua is a major vegetable pest which is stressed by heavy metals mainly by feeding.In this study,cadmium accumulation in the tissues of S.exigua exposed to cadmium and its effects on the growth and development of the parents and the offspring were investigated.Under the stress of different concentrations of cadmium(0.2,3.2,and 51.2 mg kg^(-1)),the cadmium content in each tissue of S.exigua increased in a dose-dependent manner.At the larval stage,the highest cadmium accumulation was found in midgut in all three cadmium treatments,but at the adult stage,the highest cadmium content was found in fat body.In addition,the cadmium content in ovaries was much higher than in testes.When F1S.exigua was stressed by cadmium and the F_(2)generation was not fed a cadmium-containing diet,the larval survival,pupation rate,emergence rate and fecundity of the F_(2)generation were significantly reduced in the 51.2 mg kg^(-1)treatment compared to the corresponding F1generation.Even in the F_(2)generation of the 3.2 mg kg^(-1)treatment,the fecundity was significantly lower than in the parental generation.The fecundity of the only-female stressed treatment was significantly lower than that of the only-male stressed treatment at the 3.2 and 51.2 mg kg^(-1)cadmium exposure levels.When only mothers were stressed at the larval stage,the fecundity of the F_(2)generation was significantly lower than that of the F1generation in the 51.2 mg kg^(-1)treatment,and it was also significantly lower than in the 3.2 and 0.2 mg kg^(-1)treatments.The results of our study can provide useful information for forecasting the population increase trends under different heavy metal stress conditions and for the reliable environmental risk assessment of heavy metal pollution. 展开更多
关键词 heavy metal pollution CADMIUM Spodoptera exigua tissue distribution reproduction
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EcR/USP-1-mediated ecdysteroid signaling regulates wolf spider(Pardosa pseudoannulata) development and reproduction
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作者 Zhi-Ming Yang Yang-Yang Yan +2 位作者 Yong Wu Na Yu Ze-Wen Liu 《Zoological Research》 SCIE CAS CSCD 2023年第1期43-52,共10页
Lycosidae females demonstrate meticulous maternal care of offspring by carrying egg sacs and juvenile spiderlings during the reproductive stage. Nuclear receptors(NRs), especially the ecdysone receptor(EcR) and ultras... Lycosidae females demonstrate meticulous maternal care of offspring by carrying egg sacs and juvenile spiderlings during the reproductive stage. Nuclear receptors(NRs), especially the ecdysone receptor(EcR) and ultraspiracle(USP), have attracted considerable attention in the regulation of arthropod development and reproduction due to their pivotal roles in ecdysteroid signaling cascades. In the present study, 23 NRs, including one EcR and two USPs, were identified in the genome of the predatory wolf spider Pardosa pseudoannulata. RNA interference(RNAi) targeting EcR and USP-1inhibited spiderling development and resulted in nonviable eggs in the egg sacs. EcR and USP-1responded to changes in ecdysteroid levels, and interference in ecdysteroid biosynthesis led to similar phenotypes as ds EcR and ds USP-1 treatments.These findings suggest that EcR/USP-1-mediated ecdysteroid signaling regulates P. pseudoannulata development and reproduction. The P.pseudoannulata females with suppressed ecdysteroid signaling proactively consumed their non-viable egg sacs, resulting in a 7.19 d shorter first reproductive cycle than the controls. Termination of the failed reproductive cycle enabled the spiders to produce a new egg sac more rapidly. This reproductive strategy may partially rescue the reduction in population growth due to non-viable eggs and compensate for the physiological expenditure of wasted maternal care, which would be beneficial for the conservation of P.pseudoannulata populations and their natural control of insect pests. 展开更多
关键词 Pardosa pseudoannulata Ecdysone receptor ULTRASPIRACLE DEVELOPMENT reproduction
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Russian Collaborative Development of Reproduction Technologies for the Sustainable Management of Amphibian Biodiversity
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作者 Victor K.UTESHEV Edith N.GAKHOVA +5 位作者 Ludmila I.KRAMAROVA Natalia V.SHISHOVA Svetlana A.KAUROVA Elena A.KIDOVA Artem A.KIDOV Robert K.BROWNE 《Asian Herpetological Research》 SCIE CSCD 2023年第1期103-115,共13页
Reproduction technologies(RTs)can provide for the reliable reproduction of amphibians,as well as perpetuation of species genetic variation with the use of biobanks.In 1982,in anticipation of the biodiversity conservat... Reproduction technologies(RTs)can provide for the reliable reproduction of amphibians,as well as perpetuation of species genetic variation with the use of biobanks.In 1982,in anticipation of the biodiversity conservation crisis,major Russian institutions collaborated in a dynamic program to develop and implement RTs for the sustainable management of amphibian biodiversity.An initial primary focus was the captive breeding of threatened Russian endemic anuran and caudate species,using RTs that varied from environmental manipulation to the use of exogenous gonadotropic hormones to stimulate reproduction.These species were mostly from Palearctic or cool mountain regions,but also included a wide range of species from warm regions.Other early achievements included the successful cryopreservation of anuran spermatozoa and anuran diploid pluripotent cell nuclei,in order to store both the matrilineal and patrilineal genomes in biobanks,with their subsequent development to the blastula stage after implantation into enucleated oocytes.After the turn of the 21st Century,in support of the priorities of the Amphibian Conservation Action Plan(2007),we developed RTs for the refrigerated storage of testicular or urinary spermatozoa for days to weeks at 4℃,the cryopreservation of urinary spermatozoa using anovel cryoprotectant,the in vitro fertilisation of hormonally induced oocytes either fresh or after refrigerated ex situ or in situ storage,and the artificial insemination of salamanders with fresh spermatozoa.In this article,we describe previously unpublished techniques and techniques from obscure Russian sources. 展开更多
关键词 artificial fertilisation assisted reproductive technologies(ART) CRYOPRESERVATION gonadotropic hormones OOCYTES SPERMATOZOA storage urinary spermatozoa
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Performance in Survival,Development and Reproduction of Aphis glycines Matsumura Virginoparae at High Temperatures
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作者 Liu Jian Liu Zhe +3 位作者 Wu Ci-rui Liu Dai-lin Huo Dong-bo Sun Wen-peng 《Journal of Northeast Agricultural University(English Edition)》 CAS 2023年第1期20-27,共8页
The soybean aphid,Aphis glycines Matsumura,is an important pest of soybean,which is native to Asia.In this study,A.glycines fed on soybean(AgFS)and A.glycines fed on wild soybean(AgFW)were reared at 25℃,27℃,29℃,31... The soybean aphid,Aphis glycines Matsumura,is an important pest of soybean,which is native to Asia.In this study,A.glycines fed on soybean(AgFS)and A.glycines fed on wild soybean(AgFW)were reared at 25℃,27℃,29℃,31℃,33℃and 35℃,respectively,and some of the life parameters were determined.At temperature ranging from 25℃to 31℃,nymphs of AgFS and AgFW all developed into adults successfully.Only a few nymphs of AgFS and AgFW developed into adults at 33℃and no nymphs could develop into adults at 35℃.Lifespan,fecundity and body size of AgFS and AgFW adults all decreased gradually with temperatures increasing from 25℃to 33℃.At 25℃,the intrinsic rate of increase of AgFS was as big as that at 27℃,which was smaller than that at 29℃,but was bigger than that at 31℃.Intrinsic rate of increase of AgFW decreased gradually with temperatures increasing from 25℃to 31℃.Nymph stage duration of AgFW was longer than or as long as that of AgFS;adult lifespan of AgFW was shorter than or as long as that of AgFS.Adult fecundity,intrinsic rate of increase and adult body size of AgFW were all smaller than or as big as those of AgFS.It showed that AgFS and AgFW both survived and developed well at temperature ranging from 25℃to 31℃,and AgFW was more adaptive to low temperatures.These results were important to study the adaptability of A.glycines to high temperatures and for predicting its dynamics in the temperature keeping rising region. 展开更多
关键词 Aphis glycines Matsumura DEVELOPMENT reproduction high temperature
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Scale Breeding and Reproduction Technique of Ficus tikoua Bur.Container Seedlings
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作者 Tinghong TAN Fan GAO +5 位作者 Yang XIAO Mingxing DAI Shunping BAI Shimei LIAO Chaojun FENG Chunfang WU 《Plant Diseases and Pests》 CAS 2023年第5期16-18,23,共4页
Based on the principle of asexual reproduction,a kind of scale breeding and reproduction technique of Ficus tikoua Bur.container seedlings was explored by using the characteristics of strong adaptability to the enviro... Based on the principle of asexual reproduction,a kind of scale breeding and reproduction technique of Ficus tikoua Bur.container seedlings was explored by using the characteristics of strong adaptability to the environment and fast growth and reproduction.Using non-woven bag as a breeding container for seedlings,the scale breeding and reproduction technique of F.tikoua container seedlings was summarized through the important links of seedling bed construction,seedling collection,soil configuration,container selection,cutting cultivation,field management,and disease and pest control.This technique can achieve differential,massive and sustainable efficient breeding and reproduction of F.tikoua seedlings in a short time. 展开更多
关键词 Ficus tikoua Bur Pharmaceutical/food resource Asexual reproduction Container seedling Scale breeding and reproduction
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Anti-Müllerian Hormone and Its Utility in Cattle Reproduction
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作者 Garza Arredondo Aimé Jazmín Moreno Degollado Gustavo +4 位作者 Adolfo-Soto Dominguez Pérez Hernández Raymundo Alejandro Rosales S. Cedillo Zamora-Ávila Diana Castillo-Velázquez Uziel 《Open Journal of Veterinary Medicine》 CAS 2023年第1期1-11,共11页
Reproductive biotechnologies offer us greater possibilities to improve animal genetics. However, the success of these depends on different factors such as the proper selection of the donor female. For this reason, end... Reproductive biotechnologies offer us greater possibilities to improve animal genetics. However, the success of these depends on different factors such as the proper selection of the donor female. For this reason, endocrine markers have been used to evaluate the ovarian reserve, which allow a successful selection of donors. Recent research has shown, among other things, that concentrations greater than 0.130 ng/mL of anti-Mullerian hormone (also known as Muller-inhibiting substance, which is a member of the transforming growth factor beta superfamily of growth and differentiation factors) are related to donors of more than fifteen transferable embryos. Therefore, this review describes studies showing that the measurement of anti-Müllerian hormone concentrations, before superovulation programs, reduces the costs per embryo produced. 展开更多
关键词 AMH Anti-Müllerian Hormone Reproductive Techniques Follicular Population Granulosa Cells SUPEROVULATION
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Effects of Different Levels of Garlic Powder Incorporated in the Feed Enriched with Stylosanthes guianensis on Reproduction and Pre-Weaning Growth Performances of Cavies (Cavia porcellus)
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作者 Martin Vidal Tatang Emile Miégoué +6 位作者 Margaret Mary Momo Chongsi Wouafo David Fokom Nyah Cédric Kwayep Paulette Ntsafack Tobou France-Gina Djoumessi Mathieu Duclos Fogang Fernand Tendonkeng 《Open Journal of Animal Sciences》 2023年第4期574-589,共16页
In order to increase the productivity of the local cavies as a meat animal, a study was carried out between November 2021 and March 2022 at the Research and Experimental Farm, Animal Nutrition and Production Research ... In order to increase the productivity of the local cavies as a meat animal, a study was carried out between November 2021 and March 2022 at the Research and Experimental Farm, Animal Nutrition and Production Research Unit of the Faculty of Agronomy and Agricultural Sciences (FAAS) and the Microbiology and Anti-Microbial Research Unite of the University of Dschang on 72 cavies aged between 4 and 5 months and weighing an average of 450 ± 50 g produced on the farm, including 60 females and 12 males. The females were distributed according to a completely randomized factorial design in twelve (12) rearing boxes numbered G1 to G12, with 15 females per group distributed in 03 boxes and receiving iso-nitrogenated experimental diet (18% protein) R0 enriched with 20% of Stylosanthes guianensis (0% garlic powder), R0.25 (0.25% garlic powder), R0.50 (0.50% garlic powder) and R0.75 (0.75% garlic powder). Females were identified by numbered ear tags and mated with a sex ratio of 5 females for 1 male. Drinking water enriched with vitamin C (01 tablet of 240 mg in 1.5 liters of water) was served ad libitum and renewed daily. The males were removed after 31 days. A form was established to record the weight of each pregnant female, any abortions during gestation, the date of parturition and the date of birth of each newborn, as well as the number of live-born or stillborn pups. After parturition, the weights of female and newborn cavies were recorded weekly until the 3<sup>rd</sup> week. The number of cavies that died before weaning and the number of live weaned cavies were also recorded. Reproductive performance significantly increased (p 0.05) fertility rate and net fertility rate, litter size, birth viability rate, and pre-weaning viability rate with rations containing garlic powder, compared to the control ration, especially with the R0.50 ration. In addition, birth mortality and pre-weaning mortality were significantly lower (p 0.05) with rations containing the additive, compared to those of the control ration (R0). However, the R0.50 ration showed the lowest rates, followed by the R0.25 ration, and finally the R0.75 ration. Weight development of young cavies increased with all rations but was more marked with the R0.50 ration. Only total weight gain (TG) and average daily weight gain (ADG) in the pre-weaning phase were significantly increased (p < 0.05) with rations R0.25 and R0.50. 展开更多
关键词 Cavy Garlic Powder Pre-Weaning Growth reproduction Stylosanthes guianensis
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耦合水文情势及鱼类繁殖的江垭水库生态调度需求研究 被引量:2
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作者 徐薇 魏秘 +3 位作者 曹俊 蔡露 高少波 朱迪 《水生态学杂志》 CSCD 北大核心 2024年第1期103-111,共9页
为了修复河流水文情势、维持河流生态系统健康,通过环境DNA采样、IHA水文指标变动分析和文献资料总结等方法,开展了江垭水库坝下水文情势及鱼类繁殖需求关系研究,提出了水库生态调度需求与建议。结果显示,2021年9月在溇水干支流综合调... 为了修复河流水文情势、维持河流生态系统健康,通过环境DNA采样、IHA水文指标变动分析和文献资料总结等方法,开展了江垭水库坝下水文情势及鱼类繁殖需求关系研究,提出了水库生态调度需求与建议。结果显示,2021年9月在溇水干支流综合调查获得鱼类55种,与20世纪90年代相比,流水性种类在干流江段显著减少了51.35%;按照生态调度优先等级评估原则,确定江垭水库生态调度的主要目标物种为产漂流性卵的银鲴(Xenocypris argentea)、银鮈(Squalidus argentatus)、贝氏䱗(Hemiculter bleekeri)和产粘沉性卵的鲤(Cyprinus carpio)、鲫(Carassius auratus);江垭建坝前后下游水文情势变化较大,发生高度改变且对鱼类洄游产卵及鱼卵发育有重要作用的水文指标包括年最大1日、3日平均流量指标、年出现高流量脉冲事件的次数和持续时间以及连续日流量上涨率。研究表明,以促进坝下不同产卵类型的鱼类繁殖为目标,生态调度需要一定的高流量脉冲次数和历时,还需要控制下游水位的日降幅,通过泄放合理的生态流量来维持下游鱼类的生物多样性。 展开更多
关键词 鱼类繁殖 环境DNA 水文情势 生态调度 江垭水库
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薹草属植物的生物学研究进展 被引量:1
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作者 王竞红 张靖楠 +4 位作者 覃崇源 谭卓然 罗语菲 蔺吉祥 王非 《中国草地学报》 CSCD 北大核心 2024年第4期120-131,共12页
薹草属(Carex)隶属于莎草科,是被子植物中最大的属之一。该属植物种类繁多,可见于林下、林缘、湿地、草甸等多种生境,分布较为广泛。该属内许多薹草种株型优美,抗性较强,在园林植物造景、盐碱地绿化和湿地植被修复等方面起着重要的作用... 薹草属(Carex)隶属于莎草科,是被子植物中最大的属之一。该属植物种类繁多,可见于林下、林缘、湿地、草甸等多种生境,分布较为广泛。该属内许多薹草种株型优美,抗性较强,在园林植物造景、盐碱地绿化和湿地植被修复等方面起着重要的作用,因而近年来逐渐受到人们的关注。基于此,本文从薹草属植物的系统分类、抗逆性与栽培繁殖3个方面对其分类依据、新种发现情况、不同非生物因子的影响以及不同繁殖方式和养护特点进行了总结,并提出了研究展望。以期为薹草属植物应用于园林绿化与生态修复提供理论依据,也为薹草属植物后续在抗逆机理、种子萌发和快速繁殖等方面深入研究提供思路。 展开更多
关键词 薹草属 形态特征 系统分类 抗逆性 繁殖
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低温胁迫下水稻孕穗期幼穗的转录组动态分析 被引量:1
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作者 郭震华 蔡丽君 +8 位作者 潘国君 王立楠 周雪松 杜晓东 蔡永盛 张希瑞 韩笑 周通 马文东 《种子》 北大核心 2024年第8期60-68,78,共10页
为深入了解寒地水稻孕穗期耐低温机理,明确低温应答的分子机制,本研究对孕穗期耐冷性强的水稻品种龙粳25(LG25)的幼穗进行低温胁迫转录组动态分析。通过测序,共获得515.26 Mb的Clean Reads,筛选获得6927个差异表达基因(DEGs)。其中,低... 为深入了解寒地水稻孕穗期耐低温机理,明确低温应答的分子机制,本研究对孕穗期耐冷性强的水稻品种龙粳25(LG25)的幼穗进行低温胁迫转录组动态分析。通过测序,共获得515.26 Mb的Clean Reads,筛选获得6927个差异表达基因(DEGs)。其中,低温处理2 d时,获得6784个DEGs;低温处理4 d后,DEGs显著减少,仅获得1149个。GO富集分析表明,韦恩图各部分的上调和下调基因分别显著富集到33个生物进程GO类别,其中包含基因数最多的类别为单生物代谢过程,低温应答和高温应答在LG25的韦恩图各部分富集最多。KEGG富集分析表明,韦恩图各部分DEGs共富集到32条信号通路,其中植物激素信号转导通路在韦恩图各部分显著富集最多,共包含83个DEGs,分布在脱落酸、生长素、油菜素内酯、细胞分裂素、赤霉素、乙烯、茉莉酸、水杨酸等8个激素通路中,以生长素、脱落酸和水杨酸等激素包含的DEGs较多,分别为26个、17个和15个。 展开更多
关键词 寒地水稻 孕穗期 低温 转录组 植物激素
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猪繁殖与呼吸综合征病毒入侵宿主细胞途径研究进展 被引量:2
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作者 李睿 乔松林 张改平 《中国兽医杂志》 CAS 北大核心 2024年第3期80-83,共4页
病毒入侵宿主细胞是其完成自身复制周期的首要步骤。全面解析病毒入侵宿主细胞途径可深入揭示病毒感染及其致病机制,有助于制定更为有效的抗病毒策略。猪繁殖与呼吸综合征病毒(PRRSV)是具有囊膜的单股正链RNA病毒,对全球养猪业造成巨大... 病毒入侵宿主细胞是其完成自身复制周期的首要步骤。全面解析病毒入侵宿主细胞途径可深入揭示病毒感染及其致病机制,有助于制定更为有效的抗病毒策略。猪繁殖与呼吸综合征病毒(PRRSV)是具有囊膜的单股正链RNA病毒,对全球养猪业造成巨大的经济损失。以往研究报道PRRSV主要通过网格蛋白介导的内吞途径(CME)入侵宿主细胞。近年来研究发现,除CME外,PRRSV还会采取其他途径入侵宿主细胞。本文通过总结PRRSV入侵宿主细胞途径的最新研究进展,以期加深人们对PRRSV的认识,并为防控该病毒提供新的思路。 展开更多
关键词 猪繁殖与呼吸综合征病毒(PRRSV) 入侵 内吞 巨胞饮 膜融合
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打造产教融合新空间:市域产教联合体建设的理论基础、原则与优化路径 被引量:1
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作者 李炎炎 方益权 +1 位作者 池春阳 刘海明 《职教论坛》 北大核心 2024年第5期44-50,共7页
创新搭建产教融合平台载体,形成地方政府、产业园区、高校、企业、科研院所等多元行动主体创新互动、人才共培、成果同享的共生空间,完善校政企协同“以教促产、以产助教”机制,是深化现代职业教育体系改革的重要举措,更是创新驱动发展... 创新搭建产教融合平台载体,形成地方政府、产业园区、高校、企业、科研院所等多元行动主体创新互动、人才共培、成果同享的共生空间,完善校政企协同“以教促产、以产助教”机制,是深化现代职业教育体系改革的重要举措,更是创新驱动发展战略和区域协调发展战略对职业教育提出的新要求和新方向。市域产教联合体应以产业园区为基础,以价值共创、多元共生、文化共塑为原则,形成主体共识、实现多元治理、推动联合协同,打造增强职业教育服务区域高质量发展的多主体共同成长、边界突破、场景驱动的异质行动者产教融合新空间。 展开更多
关键词 市域产教联合体 行动者 空间再生产 多元主体
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不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响 被引量:2
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作者 刘静 孙海霞 +5 位作者 韩书宇 高启楠 臧素敏 谢辉 刘观忠 安胜英 《饲料研究》 CAS 北大核心 2024年第7期34-38,共5页
试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组... 试验旨在探究“2+2”生产模式下不同粗蛋白质水平对白王种鸽繁殖性能和乳鸽生长性能的影响。试验选取产蛋性能相近的300日龄健康种鸽168对,随机分为4组,每组7个重复,每个重复6对种鸽,每对种鸽哺育2只乳鸽。试验Ⅰ组、Ⅱ组、Ⅲ组和Ⅳ组分别饲喂粗蛋白质水平为13.43%、15.04%、16.64%和18.25%的饲粮。试验期46 d,其中孵化期18 d (1~18 d),哺乳期28 d (19~46 d)。结果显示,试验Ⅱ组、Ⅲ组产蛋间隔显著低于试验Ⅰ组(P<0.05),试验Ⅲ组种蛋受精率显著高于试验Ⅰ组、Ⅳ组(P<0.05),试验Ⅲ组入孵蛋孵化率极显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.01)。试验Ⅲ组乳鸽28日龄体重显著高于试验Ⅰ组(P<0.05),乳鸽屠宰率极显著高于试验Ⅰ组(P<0.01),乳鸽胸肌率显著高于试验Ⅰ组、Ⅱ组、Ⅳ组(P<0.05)。试验Ⅱ组、Ⅲ组、Ⅳ组窝料重比1极显著低于试验Ⅰ组(P<0.01)。研究表明,不同粗蛋白质水平影响了种鸽的繁殖性能、乳鸽的生长性能以及窝料重比,在“2+2”生产模式下,建议白王种鸽饲粮适宜粗蛋白质水平为16.64%。 展开更多
关键词 种鸽 乳鸽 粗蛋白质水平 “2+2”生产模式 繁殖性能 生长性能
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剖宫产子宫瘢痕缺损对采用辅助生殖技术孕妇妊娠结局的影响及对策 被引量:1
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作者 王玲 赵晨含 章勤 《浙江大学学报(医学版)》 CAS CSCD 北大核心 2024年第3期313-320,共8页
近二十年中国的剖宫产率持续上升,导致继发性不孕的剖宫产瘢痕缺损(CSD)患者增加,需要通过辅助生殖技术(ART)解决再生育问题。CSD可显著降低ART活产率、临床妊娠率和胚胎植入率,这与患者子宫内膜容受性下降、瘢痕局部发生腺肌病及子宫... 近二十年中国的剖宫产率持续上升,导致继发性不孕的剖宫产瘢痕缺损(CSD)患者增加,需要通过辅助生殖技术(ART)解决再生育问题。CSD可显著降低ART活产率、临床妊娠率和胚胎植入率,这与患者子宫内膜容受性下降、瘢痕局部发生腺肌病及子宫内膜异位症、宫腔微环境紊乱、ART操作难度及妊娠并发症增加有关。除了使用促性腺激素释放激素类似物可能改善妊娠结局,宫腔镜手术、腹腔镜手术和经阴道手术是治疗CSD的有效方法,可提高妊娠率。本文通过文献回顾,分析CSD对采用ART孕妇妊娠结局的影响及相应的治疗方法,以期为CSD患者管理及改善ART妊娠结局提供新思路。 展开更多
关键词 剖宫产 剖宫产瘢痕缺损 辅助生殖技术 妊娠结局 综述
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帕金森病患者认知和运动功能与女性生殖因素及血脂的相关性分析 被引量:1
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作者 张宁 腾飞 孙嫄 《中国实用神经疾病杂志》 2024年第1期95-98,共4页
目的探讨帕金森病(PD)患者认知、运动功能与女性生育及绝经年龄、血脂等的相关性。方法选取2019-05—2022-11徐州市中心医院92例女性PD患者为研究组,另选取同一时间段门诊体检的101例健康女性为对照组,比较2组生殖因素及血脂水平。结果... 目的探讨帕金森病(PD)患者认知、运动功能与女性生育及绝经年龄、血脂等的相关性。方法选取2019-05—2022-11徐州市中心医院92例女性PD患者为研究组,另选取同一时间段门诊体检的101例健康女性为对照组,比较2组生殖因素及血脂水平。结果研究组绝经年龄[(47.8±8.2)岁比(50.2±9.8)岁]、生育年龄[(31.6±4.1)岁比(34.9±6.5)岁]及TC[(4.6±1.0)mmol/L比(5.8±1.5)mmol/L]、TG[(1.3±0.2)mmol/L比(1.8±0.6)mmol/L]、HDL-C[(1.2±0.3)mmol/L比(1.5±0.4)mmol/L])、LDL-C水平[(3.1±0.5)mmol/L比(3.8±0.9)mmol/L]均低于对照组(P<0.05),认知功能评分显著低于对照组[(24.2±5.6)分比(28.9±1.6)分,P<0.05],运动功能评分显著高于对照组[(28.9±5.6)分比(12.5±1.6)分,P<0.05]。PD患者绝经年龄、生殖年龄及TC、TG、HDL-C、LDL-C水平均与认知功能评分呈正相关(r=4.172、3.518、4.183、3.829、4.963、3.829,P<0.05),与运动功能评分呈负相关(r=—3.289、—4.172、—3.818、—4.210、—3.826、—4.162,P<0.05)。结论PD的发生与生殖因素、血脂水平存在一定的相关性,维持一定的血脂水平、保持雌激素水平稳定可能会一定程度上预防PD的发生。 展开更多
关键词 帕金森病 认知功能 运动功能 血脂 生殖因素 女性
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叙事医学联合案例教学法在生殖医学教学中的应用 被引量:2
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作者 朱素芹 孙艳 +2 位作者 周园 姜雯雯 郑备红 《卫生职业教育》 2024年第9期55-58,共4页
目的探索叙事医学联合案例教学法在生殖医学本科生教学中的应用效果。方法选取2022年3—12月福建医科大学两个班的临床医学本科生作为研究对象,在生殖医学教学中实行分组教学。实验组(n=90)采用叙事医学联合案例教学法,对照组(n=90)采... 目的探索叙事医学联合案例教学法在生殖医学本科生教学中的应用效果。方法选取2022年3—12月福建医科大学两个班的临床医学本科生作为研究对象,在生殖医学教学中实行分组教学。实验组(n=90)采用叙事医学联合案例教学法,对照组(n=90)采用传统以授课为主的教学法。通过理论知识、操作技能考核及满意度问卷调查评估两种教学方法的效果。结果实验组生殖医学理论知识、技能操作和生殖法律法规成绩均高于对照组(P<0.05),教学满意度评分也高于对照组(P<0.05)。结论叙事医学联合案例教学法可以有效提升生殖医学本科生的教学质量和教学满意度,值得推广。 展开更多
关键词 生殖医学 叙事医学 案例教学
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数字经济赋能海洋经济高质量发展 被引量:1
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作者 马文婷 邢文利 高若 《经济问题》 北大核心 2024年第6期42-50,共9页
海洋经济高质量发展是加快中国海洋强国建设的基础和关键。海洋经济高质量发展要求实现从“量的积累”迈向“质的飞跃”,更好地满足人民日益增长的美好生活需要。近年来中国海洋经济增速放缓,海洋经济领域固有矛盾严重阻滞了其高质量发... 海洋经济高质量发展是加快中国海洋强国建设的基础和关键。海洋经济高质量发展要求实现从“量的积累”迈向“质的飞跃”,更好地满足人民日益增长的美好生活需要。近年来中国海洋经济增速放缓,海洋经济领域固有矛盾严重阻滞了其高质量发展,急需运用新的方式加以整改。而自人类进入数字时代以来,数字经济已成为助推经济内容变革、速率变革和动力变革的“提速器”,中国作为世界第二大数字经济体,需要发挥数字经济功用从而打破传统时空概念对海洋经济活动的限制与束缚,缓解资源错配、时间错配、空间错配等问题,赋予海洋经济更多可能性的同时推动其高质量发展。对数字经济与海洋经济高质量发展的双向联动作用进行分析,从马克思社会再生产理论对赋能机理加以阐释,同时深入分析二者融合现状,并提出相应的政策建议,对推进海洋强国和中国式现代化建设具有重要的理论意义和实践指引价值。 展开更多
关键词 数字经济 海洋经济 高质量发展 社会再生产理论
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