As a national institution for scientific research and a component of the national innovation system, CAS should and must make key contributions to the great national rejuvenation of the country. Keeping this in mind, ...As a national institution for scientific research and a component of the national innovation system, CAS should and must make key contributions to the great national rejuvenation of the country. Keeping this in mind, CAS has developed four developmental targets for its basic research. This was revealed at a CAS conference on basic research held June 11-12 in Beijing.展开更多
Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answeri...Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answering basic questions about their functions and mechanisms.It takes advantages of physical methodologies to gain quantitative understanding of biological processes,often engaging precise physical measurements of reconstructed objects to avoid interference from unnecessary complications.In this review,we(i)briefly introduce concepts of single-molecule physical biology,(ii)describe extensively used single-molecule methodologies that have been developed to address key questions in two important objects of single-molecule physical biology,namely,nucleic acid-interacting proteins and membraneinteracting proteins,and(iii)show by a few successful examples how one may use single-molecule methods to deepen our understanding of protein machines.展开更多
One of the key goals of the FAIR guiding principles is defined by its final principle-to optimize data sets for reuse by both humans and machines.To do so,data providers need to implement and support consistent machin...One of the key goals of the FAIR guiding principles is defined by its final principle-to optimize data sets for reuse by both humans and machines.To do so,data providers need to implement and support consistent machine readable metadata to describe their data sets.This can seem like a daunting task for data providers,whether it is determining what level of detail should be provided in the provenance metadata or figuring out what common shared vocabularies should be used.Additionally,for existing data sets it is often unclear what steps should be taken to enable maximal,appropriate reuse.Data citation already plays an important role in making data findable and accessible,providing persistent and unique identifiers plus metadata on over 16 million data sets.In this paper,we discuss how data citation and its underlying infrastructures,in particular associated metadata,provide an important pathway for enabling FAIR data reuse.展开更多
文摘As a national institution for scientific research and a component of the national innovation system, CAS should and must make key contributions to the great national rejuvenation of the country. Keeping this in mind, CAS has developed four developmental targets for its basic research. This was revealed at a CAS conference on basic research held June 11-12 in Beijing.
基金supported by the National Key Research and Development Program of China(Grant No.2019YFA0709304)the National Natural Science Foundation of China(Grant Nos.12090051 and 12022409)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB37000000)the Youth Innovation Promotion Association of CAS(Grant Nos.2021009 and Y2021003)
文摘Physical biology is an interdisciplinary field that bridges biology with physical sciences and engineering.Singlemolecule physical biology focuses on dynamics of individual biomolecules and complexes,aiming to answering basic questions about their functions and mechanisms.It takes advantages of physical methodologies to gain quantitative understanding of biological processes,often engaging precise physical measurements of reconstructed objects to avoid interference from unnecessary complications.In this review,we(i)briefly introduce concepts of single-molecule physical biology,(ii)describe extensively used single-molecule methodologies that have been developed to address key questions in two important objects of single-molecule physical biology,namely,nucleic acid-interacting proteins and membraneinteracting proteins,and(iii)show by a few successful examples how one may use single-molecule methods to deepen our understanding of protein machines.
基金This work was partially supported by Horizon 2020,INFRADEV-4-2014-2015,654248,CORBEL,Coordinated Research Infrastructures Building Enduring Life-science services.
文摘One of the key goals of the FAIR guiding principles is defined by its final principle-to optimize data sets for reuse by both humans and machines.To do so,data providers need to implement and support consistent machine readable metadata to describe their data sets.This can seem like a daunting task for data providers,whether it is determining what level of detail should be provided in the provenance metadata or figuring out what common shared vocabularies should be used.Additionally,for existing data sets it is often unclear what steps should be taken to enable maximal,appropriate reuse.Data citation already plays an important role in making data findable and accessible,providing persistent and unique identifiers plus metadata on over 16 million data sets.In this paper,we discuss how data citation and its underlying infrastructures,in particular associated metadata,provide an important pathway for enabling FAIR data reuse.
