Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeut...Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.展开更多
Pasteurellosis is the most prevalent, extremely contagious bacterial disease among domestic rabbits and is considered the leading cause of deaths in rabbits, resulting in enormous economic losses to the rabbit industr...Pasteurellosis is the most prevalent, extremely contagious bacterial disease among domestic rabbits and is considered the leading cause of deaths in rabbits, resulting in enormous economic losses to the rabbit industry. Screening for bacterial agents causing mortalities in rabbits revealed the presence of Enterobacteriacae species in approximately 42% of studied cases, with E. coli the most commonly isolated organism. The present study was designed to evaluate the immune response of rabbits vaccinated with a locally prepared, combined inactivated vaccine of Pasteurella multocida and E. coli, adjuvanated with Montanide ISA70. A total of 370 rabbits, aged 2 - 3 weeks, were divided into four groups: (G1) vaccinated with a polyvalent P. multocida vaccine, (G2) vaccinated with a polyvalent E. coli vaccine, (G3) vaccinated with a combined inactivated Montanide ISA70 vaccine of P. multocida and E. coli, and (G4) kept as a non-vaccinated control group. All rabbits received two doses of 0.5 ml of the prepared vaccines, administered one month apart, and were then challenged with virulent strains of P. multocida and E. coli three weeks after the second vaccination. The prepared vaccines were evaluated by determining humoral immunity using indirect haemagglutination (IHA) test and ELISA. The potency of the vaccines was assessed through challenge and determination of LD50. Experimental findings on the prepared polyvalent combined inactivated P. multocida and E. coli vaccine indicated that it is a potent vaccine, producing the highest antibody titers and a 90% protection rate against challenges with virulent strains of P. multocida type A, D2, and E. coli types O157, O151 and O125. Thus, this vaccine is promising in addressing both P. multocida and E. coli problems in rabbits, farms, providing significant protection, and we recommend its commercial production to help rabbit producers control these two major bacterial infections.展开更多
The paper is to determine the types of approaches preferred by medical and pharmacy directors of US payer organizations to enhance the Pharmacy ＆ Therapeutic decision-making process and how medications accepted onto ...The paper is to determine the types of approaches preferred by medical and pharmacy directors of US payer organizations to enhance the Pharmacy ＆ Therapeutic decision-making process and how medications accepted onto the formulary should be covered. An online interactive survey of US medical and pharmacy directors was conducted in 2012. In addition to a 10-point Likert scale （10 = agree completely, 1 = disagree completely）, qualitative responses and interpretive analysis were used to explore beliefs about certain statements. The results showed that the 30 respondents （20 medical directors and 10 pharmacy directors） rated current progress in obtaining usable CER （comparative-effectiveness research） at only an average of 4.17 on the 10-point scale. They hoped to regularly utilize CER information in formulary decision making by 2015 （average rating, 6.03）. The rating of evidence-based medicine use in coverage decision making today was somewhat higher, at an average of 7.08 （medical directors, 7.38; pharmacy directors, 6.40）. The survey participants believe that emerging CER results will greatly affect the following areas： optimization/improvement of clinical guidelines, medical/pharmacy benefit management, assessments of the value and appropriateness of interventions, and pharmaceutical research and development. Therefore, payers expect CER to play an increasing role in helping them determine the value of new therapies.展开更多
In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and t...In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.展开更多
Theory and practice, researchers and practitioners are usually isolated in traditional education research, so much so that the research results can not solve the problems that teachers encounter in their teaching prac...Theory and practice, researchers and practitioners are usually isolated in traditional education research, so much so that the research results can not solve the problems that teachers encounter in their teaching practice. As a new mode, action research provides a bridge linking theory and teaching practice as well as a way to promote teacher development.展开更多
Video training platforms are now being implemented on a large scale in organizations. In this paper, I look at a video training platform including open educational resources available for many employees with varying p...Video training platforms are now being implemented on a large scale in organizations. In this paper, I look at a video training platform including open educational resources available for many employees with varying patterns and motivations for use. This has provided me with a research challenge to find methods that help other practitioners in the field understand and explain such initiatives. I describe ways to model the research and identify where pressures and contradictions can be found, drawing on a reflective view of my own practice in performing the research. Open educational resources are defined as technology-enabled educational resources that are openly available for consultation, use and adaptation by users for non-commercial purposes [1]. The bank subject to this case study has been the first organisation in Turkey that provided open educational resources for all its employees. The video platform (called “For @ Tube”) provides users with over 100 video lectures drawn from reputable universities around the world including Yale and Harvard. Other learning tools such as discussion forums, blogs and traditional e-learning courses have been made available to the users on the e-learning platform called “For @” since 2006. In this paper, I aim to introduce the new video training platform (“For @ Tube”) and outline some of the main research issues surrounding such an initiative. I seek to explore theoretical and practical approaches that can provide suitable tools for analysis. Activity theory is seen as a suitable approach for macroanalysis and its use is illustrated in terms of the complexity of large scale research. Activity theory, besides informing research perspectives, can be turned in upon the research process itself, allowing us to consider the challenges and context of the research. By using activity theory in this way and illustrating from a range of practical approaches, I demonstrate and illustrate a useful research approach.展开更多
Unlike consumers in the mall or supermarkets, online consumers are “intangible” and their purchasing behaviors are affected by multiple factors, including product pricing, promotion and discounts, quality of product...Unlike consumers in the mall or supermarkets, online consumers are “intangible” and their purchasing behaviors are affected by multiple factors, including product pricing, promotion and discounts, quality of products and brands, and the platforms where they search for the product. In this research, I study the relationship between product sales and consumer characteristics, the relationship between product sales and product qualities, demand curve analysis, and the search friction effect for different platforms. I utilized data from a randomized field experiment involving more than 400 thousand customers and 30 thousand products on JD.com, one of the world’s largest online retailing platforms. There are two focuses of the research: 1) how different consumer characteristics affect sales;2) how to set price and possible search friction for different channels. I find that JD plus membership, education level and age have no significant relationship with product sales, and higher user level leads to higher sales. Sales are highly skewed, with very high numbers of products sold making up only a small percentage of the total. Consumers living in more industrialized cities have more purchasing power. Women and singles lead to higher spending. Also, the better the product performs, the more it sells. Moderate pricing can increase product sales. Based on the research results of search volume in different channels, it is suggested that it is better to focus on app sales. By knowing the results, producers can adjust target consumers for different products and do target advertisements in order to maximize the sales. Also, an appropriate price for a product is also crucial to a seller. By the way, knowing the search friction of different channels can help producers to rearrange platform layout so that search friction can be reduced and more potential deals may be made.展开更多
Book, the crystallization of human wisdom and a symbol of civilization, has become an essential part of people's daily life. In the meantime, bookbinding also has significant meaning to Chines culture. The history...Book, the crystallization of human wisdom and a symbol of civilization, has become an essential part of people's daily life. In the meantime, bookbinding also has significant meaning to Chines culture. The history of bookbinding is much longer compared with that of books. It embodies Chinese Civilization and shows technology level of each dynasty in ancient China.However, due to the high-speed development of scientific economy, a brand-new way, e-read, has become the most popular reading style. As convenient as e-read, it still has drawbacks and immature characters to completely replace paper books. Yet the it has brought enormous challenge to traditional p-book. Even though e-book might cause computers' over usage and overreliance, how to overcome p-book's shortcomings, how to strengthen p-book's actual"feelings"to readers, as well as how to comprise the merits of p-book and e-book in the future, are the main content of this paper.展开更多
A family of high_order accuracy explicit difference schemes for solving 2_dimension parabolic P.D.E. are constructed. Th e stability condition is r=Δt/Δx 2=Δt/Δy 2【1/2 and the truncation err or is O(Δt 3+Δx...A family of high_order accuracy explicit difference schemes for solving 2_dimension parabolic P.D.E. are constructed. Th e stability condition is r=Δt/Δx 2=Δt/Δy 2【1/2 and the truncation err or is O(Δt 3+Δx 4).展开更多
Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,b...Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,but with longer rostrum,15-27 dorsal teeth,3-6 ventral teeth,and straighter inner margin of endopods in male 1st pleopods. C clavipes sp nov.is similar to C angustipes ,but with narrower distal and fewer spines on inner margin of endopds in male 1st pleopods,and shorter and thinner appendix masculina in male 2nd pleopods with only 1 spine on inner lateral margin and 4 spines in apex.The holotypes are deposited in Shanghai Fisheries University.Some representative paratypes are deposited in Foshan Science and Technology College.展开更多
Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was s...Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was studied in 253 cases and 67 cases respectively, the latter were pathological negative margin chosen from the former. Immunohistochemisty was used to detect the expression of eIF4E and p53 proteins. Results: The rate of pathological, p53 and eIF4E positive margins was 20.2%, 19.4% and 32.8% respectively. The recurrent rate of those with positive margins was higher than that of negative margins, which including pathological margin (70.6% vs 35.1%, P =0.0000), p53 margin (69.2% vs 33.3%, P =0.018) and eIF4E margin (63.6% vs 28.9%, P =0.018); The survival rate of those with negative margins was higher than those with positive margins, including pathological margin (the 5-year cumulative survival rate was 37.52% and 64.37% respectively, P =0.0023), p53 margin (the 5-year cumulative survival rate was 24.62% and 75.69% respectively, P =0.0012) and eIF4E margin (the 5-year cumulative survival rate was 43.31% and 77.52% respectively, P =0.0006). Conclusion: The prognosis of those with both pathological and molecular positive margins was worse than that of the negative margins; Both the eIF4E and p53 were useful markers to pick out the poor prognostic patients from those with pathological negative margin, and the former seemed to be more potential.展开更多
Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant ...Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.展开更多
The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and th...The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.展开更多
文摘Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.
文摘Pasteurellosis is the most prevalent, extremely contagious bacterial disease among domestic rabbits and is considered the leading cause of deaths in rabbits, resulting in enormous economic losses to the rabbit industry. Screening for bacterial agents causing mortalities in rabbits revealed the presence of Enterobacteriacae species in approximately 42% of studied cases, with E. coli the most commonly isolated organism. The present study was designed to evaluate the immune response of rabbits vaccinated with a locally prepared, combined inactivated vaccine of Pasteurella multocida and E. coli, adjuvanated with Montanide ISA70. A total of 370 rabbits, aged 2 - 3 weeks, were divided into four groups: (G1) vaccinated with a polyvalent P. multocida vaccine, (G2) vaccinated with a polyvalent E. coli vaccine, (G3) vaccinated with a combined inactivated Montanide ISA70 vaccine of P. multocida and E. coli, and (G4) kept as a non-vaccinated control group. All rabbits received two doses of 0.5 ml of the prepared vaccines, administered one month apart, and were then challenged with virulent strains of P. multocida and E. coli three weeks after the second vaccination. The prepared vaccines were evaluated by determining humoral immunity using indirect haemagglutination (IHA) test and ELISA. The potency of the vaccines was assessed through challenge and determination of LD50. Experimental findings on the prepared polyvalent combined inactivated P. multocida and E. coli vaccine indicated that it is a potent vaccine, producing the highest antibody titers and a 90% protection rate against challenges with virulent strains of P. multocida type A, D2, and E. coli types O157, O151 and O125. Thus, this vaccine is promising in addressing both P. multocida and E. coli problems in rabbits, farms, providing significant protection, and we recommend its commercial production to help rabbit producers control these two major bacterial infections.
文摘The paper is to determine the types of approaches preferred by medical and pharmacy directors of US payer organizations to enhance the Pharmacy ＆ Therapeutic decision-making process and how medications accepted onto the formulary should be covered. An online interactive survey of US medical and pharmacy directors was conducted in 2012. In addition to a 10-point Likert scale （10 = agree completely, 1 = disagree completely）, qualitative responses and interpretive analysis were used to explore beliefs about certain statements. The results showed that the 30 respondents （20 medical directors and 10 pharmacy directors） rated current progress in obtaining usable CER （comparative-effectiveness research） at only an average of 4.17 on the 10-point scale. They hoped to regularly utilize CER information in formulary decision making by 2015 （average rating, 6.03）. The rating of evidence-based medicine use in coverage decision making today was somewhat higher, at an average of 7.08 （medical directors, 7.38; pharmacy directors, 6.40）. The survey participants believe that emerging CER results will greatly affect the following areas： optimization/improvement of clinical guidelines, medical/pharmacy benefit management, assessments of the value and appropriateness of interventions, and pharmaceutical research and development. Therefore, payers expect CER to play an increasing role in helping them determine the value of new therapies.
文摘In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.
文摘Theory and practice, researchers and practitioners are usually isolated in traditional education research, so much so that the research results can not solve the problems that teachers encounter in their teaching practice. As a new mode, action research provides a bridge linking theory and teaching practice as well as a way to promote teacher development.
文摘Video training platforms are now being implemented on a large scale in organizations. In this paper, I look at a video training platform including open educational resources available for many employees with varying patterns and motivations for use. This has provided me with a research challenge to find methods that help other practitioners in the field understand and explain such initiatives. I describe ways to model the research and identify where pressures and contradictions can be found, drawing on a reflective view of my own practice in performing the research. Open educational resources are defined as technology-enabled educational resources that are openly available for consultation, use and adaptation by users for non-commercial purposes [1]. The bank subject to this case study has been the first organisation in Turkey that provided open educational resources for all its employees. The video platform (called “For @ Tube”) provides users with over 100 video lectures drawn from reputable universities around the world including Yale and Harvard. Other learning tools such as discussion forums, blogs and traditional e-learning courses have been made available to the users on the e-learning platform called “For @” since 2006. In this paper, I aim to introduce the new video training platform (“For @ Tube”) and outline some of the main research issues surrounding such an initiative. I seek to explore theoretical and practical approaches that can provide suitable tools for analysis. Activity theory is seen as a suitable approach for macroanalysis and its use is illustrated in terms of the complexity of large scale research. Activity theory, besides informing research perspectives, can be turned in upon the research process itself, allowing us to consider the challenges and context of the research. By using activity theory in this way and illustrating from a range of practical approaches, I demonstrate and illustrate a useful research approach.
文摘Unlike consumers in the mall or supermarkets, online consumers are “intangible” and their purchasing behaviors are affected by multiple factors, including product pricing, promotion and discounts, quality of products and brands, and the platforms where they search for the product. In this research, I study the relationship between product sales and consumer characteristics, the relationship between product sales and product qualities, demand curve analysis, and the search friction effect for different platforms. I utilized data from a randomized field experiment involving more than 400 thousand customers and 30 thousand products on JD.com, one of the world’s largest online retailing platforms. There are two focuses of the research: 1) how different consumer characteristics affect sales;2) how to set price and possible search friction for different channels. I find that JD plus membership, education level and age have no significant relationship with product sales, and higher user level leads to higher sales. Sales are highly skewed, with very high numbers of products sold making up only a small percentage of the total. Consumers living in more industrialized cities have more purchasing power. Women and singles lead to higher spending. Also, the better the product performs, the more it sells. Moderate pricing can increase product sales. Based on the research results of search volume in different channels, it is suggested that it is better to focus on app sales. By knowing the results, producers can adjust target consumers for different products and do target advertisements in order to maximize the sales. Also, an appropriate price for a product is also crucial to a seller. By the way, knowing the search friction of different channels can help producers to rearrange platform layout so that search friction can be reduced and more potential deals may be made.
文摘Book, the crystallization of human wisdom and a symbol of civilization, has become an essential part of people's daily life. In the meantime, bookbinding also has significant meaning to Chines culture. The history of bookbinding is much longer compared with that of books. It embodies Chinese Civilization and shows technology level of each dynasty in ancient China.However, due to the high-speed development of scientific economy, a brand-new way, e-read, has become the most popular reading style. As convenient as e-read, it still has drawbacks and immature characters to completely replace paper books. Yet the it has brought enormous challenge to traditional p-book. Even though e-book might cause computers' over usage and overreliance, how to overcome p-book's shortcomings, how to strengthen p-book's actual"feelings"to readers, as well as how to comprise the merits of p-book and e-book in the future, are the main content of this paper.
文摘A family of high_order accuracy explicit difference schemes for solving 2_dimension parabolic P.D.E. are constructed. Th e stability condition is r=Δt/Δx 2=Δt/Δy 2【1/2 and the truncation err or is O(Δt 3+Δx 4).
文摘Two new freshwater atyid shrimps, Caridina angustipes sp nov.and C clavipes sp nov.from Hunan Province,China are described. C angustipes sp nov.is similar to C baojingensis Guo,He et Bai,1992,but with longer rostrum,15-27 dorsal teeth,3-6 ventral teeth,and straighter inner margin of endopods in male 1st pleopods. C clavipes sp nov.is similar to C angustipes ,but with narrower distal and fewer spines on inner margin of endopds in male 1st pleopods,and shorter and thinner appendix masculina in male 2nd pleopods with only 1 spine on inner lateral margin and 4 spines in apex.The holotypes are deposited in Shanghai Fisheries University.Some representative paratypes are deposited in Foshan Science and Technology College.
文摘Objective: To study the prognostic value of the pathological margin and molecular margin marked by eIF4E and P53 protein in laryngeal carcinoma. Methods: The prognostic value of pathological and molecular margin was studied in 253 cases and 67 cases respectively, the latter were pathological negative margin chosen from the former. Immunohistochemisty was used to detect the expression of eIF4E and p53 proteins. Results: The rate of pathological, p53 and eIF4E positive margins was 20.2%, 19.4% and 32.8% respectively. The recurrent rate of those with positive margins was higher than that of negative margins, which including pathological margin (70.6% vs 35.1%, P =0.0000), p53 margin (69.2% vs 33.3%, P =0.018) and eIF4E margin (63.6% vs 28.9%, P =0.018); The survival rate of those with negative margins was higher than those with positive margins, including pathological margin (the 5-year cumulative survival rate was 37.52% and 64.37% respectively, P =0.0023), p53 margin (the 5-year cumulative survival rate was 24.62% and 75.69% respectively, P =0.0012) and eIF4E margin (the 5-year cumulative survival rate was 43.31% and 77.52% respectively, P =0.0006). Conclusion: The prognosis of those with both pathological and molecular positive margins was worse than that of the negative margins; Both the eIF4E and p53 were useful markers to pick out the poor prognostic patients from those with pathological negative margin, and the former seemed to be more potential.
文摘Consistent with previous research, results showed men get more paragraphs and photography coverage. Interestingly, women's factual information is always associated with relevant men report, including P.E relevant with personal information.
文摘The regional policy in the innovation development of private P.E economy plays better role in management according to law,guidance and escort;increase the macro quality of main body of private P.E entrepreneurs and the overall staff quality,develop P.E vocational P.E to provide P.E talents for private P.E economy.
