Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors

To explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units （OTUs）, generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant ＂biodiversity units＂. OTU composition among the sites was heterogeneous; 46.7% of the total bacterial OTUs （45） and 64.1% of the eukaryotic OTUs （39） were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages （UPGMA） clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis （CCA） was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3^--N, dissolved oxygen （DO）, and SiO3^2--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO4^3--P, and SiO3^2--Si.

Impact of Different Contour Hedgerows on Runoff,Nutrient and Soil on Sloping Farmland in Danjiangkou Reservoir Region of China

Soil and water loss has been a major environmental problem in the Danjiangkou Reservoir Region.A study of 14°sloping farmland was performed on impact of different contour hedgerows on runoff,losses of soil and nutrients during 2008 and 2011,with five treatments and three replications.The winter wheat and summer maize were used as the test crops.Treatments consisted of four hedgerows:Amorpha(Amorpha fruticosa L.),Honeysuckle(Lonicera japonica Thunb.),Day-lily flower(Hemerocallis citrina Baroni.),and Sabaigrass(Eulaliopsis binata),and a control without hedgerow.Result showed that the runoff under the control treatment was much higher than that of hedgerows.Amorpha could reduce the runoff by 35.2%compared with the control.Soil losses in four hedgerows showed significant reduction in four years(e.g.,Amorpha:78.3%;Honeysuckle:77.1%).Nutrient losses in winter were much higher than that in summer,especially total nitrogen,total phosphorus and total potassium,even though there was an abundant precipitation in summer.Hedgerows greatly affected the soil and nutrient losses on slopping farmland compared with the control treatment,especially Amorpha treatment.The present study found that the Amorpha could be used as the hedgerow species for reducing soil and water loss in the Danjiangkou Reservoir Region.

Ecological Approaches to Urban Migration of the Three Gorges Reservoir Region

With the approach of the second water storage of the Three Gorges Project, urban migration has become a great concern. However, being influenced by economical, social or other factors, ecological problems have apparently become pressing. All these problems are exerting great impact upon the speed and quality of new city’s or town’s construction. By means of ecology, urban ecology, landscape ecology, human settlement science as well as the principle of sustainable development, this paper analyzes the ecological problems in urban migration, and the corresponding approaches in order to promote ecological environment construction of new cities and give an impetus to sustainable development of the whole human settlements in the Three Gorges Reservoir Region.

A Study of Soil Nutrients in the Terrace Field Changed from Mountain Slope in Three Gorges Reservoir Region

In order to accurately grasp the soil fertility in Three Gorges Reservoir Region after changing mountain slope into terrace field,we take Wushan County for example,and use the spatio-temporal substitution method to analyze the soil nutrient status and trend about the terrace field changed from mountain slope under different length of implementation.The results show that the soil fertility is generally low in the study area,and according to the soil nutrient grading standards in the second national land survey,the average content of total nitrogen,available potassium,organic matter and available phosphorus reaches Standard III,Standard III,Standard IV and Standard V,respectively,and the spatial heterogeneity of soil nutrients is small.Compared to the sloping land,the terrace field changed from mountain slope has increasing soil nutrients on the whole with the length of farming.The mean of various indicators about soil nutrients in the terrace field changed from mountain slope for 2 years is lower than in the sloping land,and there is the biggest difference in soil fertility,while the mean of various indicators about soil nutrients in the terrace field changed from mountain slope for 14 years shows a linear upward trend,and the difference in soil fertility decreases.

Using ^(137)Cs Tracing Methods to Estimate Soil Redistribution Rates and to Construct a Sediment Budget for a Small Agricultural Catchment in the Three Gorges Reservoir Region,China

Soil erosion and associated off-site sedimentation are threatening the sustainable use of the Three Gorges Dam.To initiate management intervention to reduce sediment yields,there is an increasing need for reliable information on soil erosion in the Three Gorges Reservoir Region(TGRR).The purpose of this study is to use 137 Cs tracing methods to construct a sediment budget for a small agricultural catchment in the TGRR.Cores were taken from a pond and from paddy fields,for 137 Cs measurements.The results show that the average sedimentation rate in the pond since 1963 is 1.50 g cm-2 yr-1 and the corresponding amount of sediment deposited is 1,553 t.The surface erosion rate for the sloping cultivated lands and the sedimentation rate in the paddy fields were estimated to be 3,770 t km-2 yr-1 and 2,600 t km-2 yr-1,respectively.Based on the estimated erosion and deposition rates,and the area of each unit,the post 1970 sediment budget for the catchment has been constructed.A sediment delivery ratio of 0.5 has been estimated for the past 42 years.The data indicate that the sloping cultivated lands are the primary sediment source areas,and that the paddy fields are deposition zones.The typical land use pattern(with the upper parts characterized by sloping cultivated land and the lower parts by paddy fields) plays an important role in reducing sediment yield from agricultural catchments in the TGRR.A 137 Cs profile for the sediment deposited in a pond is shown to provide an effective means of estimating the land surface erosion rate in the upstream catchment.

Evapotranspiration and humidity variations in response to land cover conversions in the Three Gorges Reservoir Region

A new land cover classification system was established for the Three Gorges Reservoir Region(TGRR) after considering the continuity of inundation and the natural characteristics of land cover. The potential evapotranspiration(PET) was predicted using a modified Penman-Monteith(P-M) model. The region's ratio of precipitation to evapotranspiration was calculated as the humidity index(HI). The data obtained was used to analyze climatic responses to land cover conversions from the perspectives of evapotranspiration and humidity variations. The results show that, from 1997 to 2009, the average annual PET increased in the early years and decreased later. In terms of overall spatial distribution, a significant reciprocal relationship appeared between annual PET and annual HI. In 1997,the annual PET was higher in the lower reaches than in the upper reaches of the TGRR, but the areas with high PET shifted substantially westward by 2003. The annual PET continued to increase in 2006, but the areas with high PET shrank by 2009. In contrast, the annual HI showed varying degrees of localized spatial variability. Over the three periods, the dominantforms of land cover conversions occurred from evergreen cover to seasonal green cover, from seasonal green cover to evergreen cover, and from seasonal green cover to seasonally inundated areas, respectively. These accounted for 48.0%, 38.4%, and 23.8% of the total areas of converted land covers in the three periods, respectively. During the period between 1997 and 2003, the main forms of land cover conversions resulted in both positive and negative growths in the average annual PET, while all of them pushed down the average annual HI. From 2003 to 2006, the reservoir region experienced neither a decrease in the annual PET nor an increase in the annual HI. The period between 2006 and 2009 saw a consistent downward trend in the annual PET and a consistent upward trend in the annual HI.

Dry cropland changes in China's Three Gorges Reservoir Region during the period 1990 to 2015

Monitoring and analyzing changes in the extent of cultivated land may inform strategic decisions on issues of environmental and food security.The dry cropland area of 12000 km^2in the Three Gorges Reservoir Region(TGRR)of China is essential for feeding the local population of^20 million,but is highly prone to soil erosion,leading to the delivery of excessive amounts of sediment and associated pollutants to the Three Gorges Reservoir(TGR),and causing serious eco-environmental consequences.Against this background,this paper used Landsat images and a digital elevation model to analyze the altitudinal distribution of,and dynamic changes in,the area of dry cropland during the period 1990 to 2015.The results suggest that dry cropland was mainly distributed in the elevation range of 200-600 m.The dry cropland area decreased from 12525.37 km^2to 11796.27 km^2during the 25-year study period,including a particularly significant decrease in the rate of decrease from 6.93 km^2/yr to 43.99 km^2/yr after 2000.The largest decline in the dry cropland area occurred in the elevation range of 600-900 m.The transformations between dry cropland and forest revealed the impact of the TGR operation on the extent of dry cropland.A total of 528.79 km^2of dry cropland with slopes>25°were converted to forest after 2000,whereas a total of 642 km^2of forest was converted to dry cropland during the study period,and these conversions mainly occurred between the elevation of 200–900 m.These spatiotemporal changes in the dry cropland area are likely to raise new issues concerning food security in the TGRR.

Soil Geochemistry Changes Induced by a Foreign Soil Reconstruction Project in Three Gorges Reservoir Region of China

Fertile topsoil was added onto the surface of barren slope land in Three Gorges Reservoir region of China in an anthropogenic process known as the foreign soil reconstruction project. The main goal of this paper was to reveal the influence of anthropogenic activities on pedogenic processes and soil classifications. Chemical weathering indices and comparative analysis were applied to discuss changes in geochemical compositions and weathering features of purplish soils(Cambisols) before and after the project. Results showed that:(1) The foreign soil reconstruction project slightly altered the major element composition of topsoil and improved the soil structure. Although the distributions of major elements in the original topsoil, original subsoil, foreign topsoil and newly constructed topsoil were all similar to that in upper continental crust, newly constructed topsoil was the most similar soil.(2) The chemical index of alteration was more sensitive than the weathering index of Parker at indicating chemical weathering status of purplish soil. The chemical weathering status of newly constructed topsoil was higher than that of the original topsoil and lower than that of foreign topsoil.(3) Anthropogenic activities may provide a promising new thought for the anthropogenic soil classification system. The scope and subgroups of Anthrosols should be extended and strengthened. Or there may be a need to combine Anthrosols and Technosols orders to create a new soil order. The results may be used for optimizing soil mellowing engineering activities and enriching the soil classification system.

Current Situation of Land Use in Three-gorges Reservoir Region in 2010

Land is rare natural resource.Production and construction of all sectors in a region must be based on land.Thus,overall research and analysis on current situation of land use can reveal scope,depth and reasonableness of land use,is helpful for analyzing internal mechanism land use change,and can reflect production scale,level and characteristics,and also can provide basis for optimization and adjustment of land use structure.Based on RS and GIS technologies,with the aid of TM image data of the Three-gorges Reservoir Region in 2010,the data of current situation of land use in Three-gorges Reservoir Region was obtained,and current land use situation was analyzed using geographimetrics and landscape ecology methods.Results show that since natural,social and economic conditions are different,land type diversity,combination type and location index of counties in the Three-gorges Reservoir Region are varied.In the land use diversity index,Xingshan County has the most single land use type(mainly forest land); in land use degree,Yuzhong District has the highest land use degree; in the integrated index of land use,Changshou District has the lowest integrated index of land use.This study is expected to provide reference and basis for formulating policies of protecting ecological environment of the Three-gorges Reservoir Region.

Land Use Zoning in Three-Gorge Reservoir Region:A Case Study of Fengdu County in Chongqing Municipality

Taking Fengdu County in Three-Gorge Reservoir region as an example,this paper applies the dynamic clustering analysis model, and divides land resource utilization of Fengdu County into three zones: Yangtze River valley economic zone,low mountains and hills agricultural zone,and low and middle agricultural,forestry and animal husbandry zone. It also analyzes current situation and existing problems in the land utilization of every zone,and finally puts forward corresponding recommendations.

Quantitative research of diagenesis:its effect on pore evolution of the Fuyu oil reservoir in the north Qijia region

From observing cores of 18 wells,identifying 175 ordinary thin sections and 61 thin casting sections,energy spectrum analyses of 37 samples,homogenization temperature measurement of fluid inclusions of 11 samples,we determine the types of diagenesis and pores of the Fuyu oil reservoir in the north Qijia region.We classified the pores and measured their plane porosity using CIA 2000,the software of rock image analysis,calculated the effect of different kinds of diagenesis on porosity,studied the controlling actions of diagenesis to pore evolution quantitatively,combined with burial history,thermal history and their diagenetic environments.Our results show that mechanical compaction and carbonate cementation are the major destructive diagenesed,developed during early diagenesis stages.The reduction in porosity by mechanical compaction and carbonate cementation are about 25% and 8%,while the destructive intensity of siliceous cementation and clay mineral cementation is relatively much smaller,i.e.,the reduction of porosity is about 2% and 0.2% Dissolution is constructive diagenesis,the increment of porosity is about 6%.There are four diagenesis evolution stages,during which the porosity reduced from 30%～38% to 2%～20%.Mechanical compaction and early cementation are the main diageneses in the early diagenesis stages,when porosity was reduced to 2%～10%.Dissolution is the main diagenesis of an A I substage of the middle diagenesis stage,when porosity increased 1%～8%.The dissolution of the A Ⅱ substage of the middle diagenesis stage affected by late cementation,raised porosity 1%～5%.The porosity varied slightly during the middle stage B.

Geological characteristics and accumulation mechanisms of the "continuous" tight gas reservoirs of the Xu2 Member in the middle-south transition region,Sichuan Basin,China

＂Continuous＂ tight gas reservoirs are those reservoirs which develop in widespread tight sandstones with a continuous distribution of natural gas. In this paper, we summarize the geological features of the source rocks and ＂＇continuous＂ tight gas reservoirs in the Xujiahe Formation of the middle- south transition region, Sichuan Basin. The source rocks of the Xul Member and reservoir rocks of the Xu2 Member are thick （Xul Member： 40 m, Xu2 Member： 120 m） and are distributed continuously in this study area. The results of drilled wells show that the widespread sandstone reservoirs of the Xu2 Member are charged with natural gas. Therefore, the natural gas reservoirs of the Xu2 Member in the middle-south transition region are ＂continuous＂ tight gas reservoirs. The accumulation of ＂continuous＂ tight gas reservoirs is controlled by an adequate driving force of the pressure differences between source rocks and reservoirs, which is demonstrated by a ＂one-dimensional＂ physical simulation experiment. In this simulation, the natural gas of＂continuous＂ tight gas reservoirs moves tbrward with no preferential petroleum migration pathways （PPMP）, and the natural gas saturation of＂continuous＂ tight gas reservoirs is higher than that of conventional reservoirs.

Complex sources of air-soil-water pollution processes in the Miyun reservoir region

The comprehensive impact of atmospheric dry deposition and wet deposition and the pollution sources of farmlands, mining areas, and towns along the Baihe River on the water quality of Miyun reservoir is investigated from the angle of the complex sources of air-soil-water pollution processes, in the context of the 1990-2001 precipitation chemical data at Shangdianzi station--a WMO regional background air pollution monitoring station 15 km far from the Miyun reservoir, in conjunction with the atmospheric dry deposition and wet deposition data of the 2002-2003 Beijing City Air Pollution Observation Field Experiment (BECAPEX). Analysis results suggest that the major ions in precipitation in the Miyun reservoir region in this period were SO, NO, NH and Ca2+; wet acid deposition quantity of Miyun reservoir in the summer half year (April to September) was greater than the quantity in the winter half year (October to March), and the annual wet acid deposition in the reservoir exhibited a rising trend with the mean 1038.45 t, the maximum 1766.31 t occurred in 1996, and the minimum 604.02 t in 1994; the long-term averaged pH of atmospheric precipitation in the Miyun reservoir region was 5.20, i.e. weakly acidic, and the interannual variation of pH values displayed a falling trend. pH values of water body at various depths in the Miyun reservoir were all greater than 7.0, but they exhibited vertical and horizontal nonhomogeneity, and at the same region pH decreased vertically with depth; the 2002 and 2003 annual dustfalls in the Miyun reservoir were 13513.08 t and 3577.64 t, respectively, and the spring dustfall was the number one in a year, accounting for the 61.91% and 44.56% of the annual totals of 2002 and 2003, respectively. Because the atmospheric dry deposition and wet depositions contain multiple types heavy metal elements and harmful elements, they to some extent exacerbated the eutrophication, acidification and potential heavy metal pollution of the reservoir water. The above comprehensive analysis results reveal the complex source characters of the air-soil-water pollution process and the multi-sphere interaction effect. Besides, summer (rainy season) is a season when local soil pollutants enter the water system of reservoir after being washed out by torrential rain or heavy precipitation, which starts the air-soil- water chaining pollution processes, and results in the water pollution of rivers and reservoirs. It is found from the statistical analysis in this paper that the water pollution of Miyun reservoir was correlated with the rain wash-out and confluent flow in the peripheral and upstream local region of the reservoir, and the pollutant concentration of the reservoir water was significantly correlated with the upstream local region precipitation. Those correlation characters reveal the effect of the air-soil-water multi-spheric interaction of reservoir water pollution process. This paper presents the point of view of the complex source analysis of reservoir water pollution and a technical approach for tracing the spatial distribution of the upstream pollution source of the water systems of reservoir.

Diageneses and Their Influences on Reservoir Properties of Chang 2 Oil Member in Renshan Region, Zhidan Oilfield, Ordos Basin

1 Introduction Yanchang Formation in Upper Triassic,Ordos basin contains the most abundant hydrocarbon resources in North China(Wang et al.,2014).The sandstones are the most important oil-bearing reservoirs in Yanchang

Reservoir Characterization of the Early Jurassic Butmah Formation Using Well Log Data in Selected Wells from Iraqi Kurdistan Region

The reservoir characterization of Butmah Formation (Early Jurassic) was studied in two wells: (AT-1) and (SH-1B) from Atrush and Shaikhan Oilfields respectively, in Iraqi Kurdistan Region. Well logs including;sonic, density, neutron, gamma ray, and resistivity, were employed to investigate the formation. The petrophysical properties, such as lithology, shale volume, porosity, water saturation, hydrocarbon saturation, and bulk water volume, were estimated using Interactive Petrophysics Software (IP). Water saturation, bulk volume of water, residual hydrocarbon, and movable hydrocarbon were plotted as Computer Processed Interpretation (CPI) for Butmah Formation in the studied wells. In order to check reliability of log data, lithology of the formation was detected using neutron-density cross plot and M-N cross plot. The results of log interpretation display that Butmah Formation is composed of dolomite, limestone with anhydrite and shale in the studied wells. The calculated shale volume shows low clay content. Therefore, Butmah Formation is considered as a clean formation in both wells. Porosity was estimated using porosity logs (sonic, density, and neutron) and had the average total porosity of (7% - 8%) in the two wells. Based on the determined petrophysical properties, particularly porosity and hydrocarbon saturation, it can be concluded that Butmah Formation seems potentially good in reserving oil.

宜昌地区灯影组深层丘滩相碳酸盐岩储层特征

宜昌地区灯影组顶部发育微生物碳酸盐岩沉积,由藻叠层石、藻球粒、凝块石等微生物丘建造,以及藻砂屑、滑塌角砾等颗粒云岩组成,属于典型台缘浅滩—台缘斜坡相沉积。顶部高能颗粒滩相带发育大量蜂窝状溶蚀孔洞,深层碳酸盐岩孔隙度平均9.81%,为优质中孔中渗碳酸盐岩储层,是鄂西地区油气勘探开发的重要层系。

Reservoir Characteristics and Favorable Area Prediction of Chang-6 Reservoir in Zhouguan Area, Ordos Basin

Based on the analysis of a large number of core samples, logging results, logging interpretation data and dynamic data in the study area, the characteristics of Chang 6 reservoir in Zhouguan area of Baihe area are studied, and the favorable reservoir areas in the study area are predicted. The results show that the lithology of Chang 6 reservoir is mainly light gray and gray fine-grained to very fine-grained feldspar lithic sandstone. The pore types are mainly residual intergranular pores and feldspar dissolved pores, including debris dissolved pores and microfractures. The porosity and permeability values are low, which belongs to low porosity-low permeability and ultra-low permeability reservoirs. According to the reservoir distribution characteristics and related data, the Chang 6 reservoir in the study area is divided into two types, mainly Class II and Class III reservoirs. The comprehensive evaluation predicts that the spatial distribution of the favorable area of Chang 6 reservoir is not uniform, but the distribution area is large, which has broad exploration and development value, and provides the necessary conditions for the distribution study of the favorable oil-bearing zone in this area and the preparation for the next exploration and development.

Landslides and Slope Aspect in the Three Gorges Reservoir Area Based on GIS and Information Value Model

Slope aspect is one of the indispensable internal factors besides lithology, relative elevation and slope degree. In this paper authors use information value model with Geo graphical Information System （GIS） technology to study how slope aspect contributes to landslide growth from Yunyang to Wushan segment in the Three Gorges Reservoir area, and the relationship between aspect and landslide growth is quantified. Through the research on 205 landslides examples, it is found that the slope contributes most whose aspect is towards south,southeast and southwest aspect contribute moderately, and other five aspects contribute little. The research result inosculates preferably with the fact. The result of this paper can provide potent gist to the construction of Three Gorges Reservoir area in future.

梯级水库下游最不利洪水地区组成法

梯级水库运行调度显著改变了下游洪水的时程分配,而现有洪水地区组成法没有考虑对工程设计和防洪调度最不利或最恶劣的情景。本文基于灾害风险理论,根据未控区间洪量及地区组成概率,提出一种最不利洪水地区组成法。雅砻江梯级水库实例分析表明:最不利地区组成法分配给下游未控区间流域的洪量总是大于同频率组成法和最可能组成法;最不利组成法推求的雅砻江出口断面1 000年一遇设计洪峰、3 d和7 d洪量分别为16 700 m^(3)/s、41.6亿和85.1亿m^(3),均大于其他洪水地区组成法估计值。理论推导和统计试验表明最不利地区组成法可合理分配洪量并兼顾下游防洪风险,具有深入研究和应用的价值。

阿姆河盆地东北部碳酸盐岩区域性地层超压成因

由于碳酸盐岩沉积成岩作用与碎屑岩不同,碳酸盐岩地层超压具有高度非均质性,然而阿姆河盆地东北部地区碳酸盐岩地层却发育了大面积区域性超压现象。本文运用实测地层压力和钻井液密度计算压力的方法,刻画了研究区碳酸盐岩地层异常超压的空间分布特征及其与上覆膏盐岩地层压力的垂向叠置关系,运用碳酸盐岩物性、压汞分析和烃源岩生烃模拟及构造挤压增压模拟等方法,从纵向与横向对比超压与常压差异分布的角度,探讨了碳酸盐岩超压成因。研究表明:盐下碳酸盐岩发育常压与强超压,强超压区大面积连续发育,与局部构造和断裂无关。盐下致密碳酸盐岩形成封闭体系,虽然构造挤压作用发育了断层与裂缝,但是由于膏盐岩的塑性,未破坏盐下碳酸盐岩封闭体系,是超压区域性连续发育的前提;同时,强烈构造挤压对超压发育有重要贡献。由于膏盐岩的良好封闭性,使膏盐岩层与碳酸盐岩层之间优质烃源岩(TOC最高达6%)生成液态烃类向盐下碳酸盐岩排烃,也促进了超压发育;由于膏盐岩与碳酸盐岩之间发育的泥岩隔层,石膏矿物脱水作用不是碳酸盐岩超压的成因,并且,石膏成岩脱水作用对盐下碳酸盐岩开放体系作用微弱。研究成果对盐下异常超压的预测具有借鉴意义。