Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two...Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay （IFA）. Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces （the exception being the Miyun area in Beijing）. The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces.展开更多
This study aims to analyze how park households perceive the economic,sociocultural,and environmental impacts of ecotourism in Simien Mountain National Park(SMNP),Ethiopia.We combined qualitative and quantitative techn...This study aims to analyze how park households perceive the economic,sociocultural,and environmental impacts of ecotourism in Simien Mountain National Park(SMNP),Ethiopia.We combined qualitative and quantitative techniques for data collection.A sequentially embedded mixed design that gives priority to quantitative data was used.We also employed a multistage sampling technique to select respondents from three districts or woredas,namely Debark,Janamora,and Beyeda woredas.Finally,397 participants were involved in the study from a random selection of three villages from each district or woreda.Data were gathered using a survey questionnaire,interviews,and focus group discussions.Data were analyzed using both descriptive and inferential statistical methods,including means,standard deviations,analysis of variance(ANOVA),and logistic regression models.The findings from a logistic regression analysis revealed that ecotourism participation had a significant relationship with gender(B=1.850,P=0.023),level of education(B=0.238,P=0.032),geographical location(B=0.420,P=0.041),and occupation(B=0.920,P=0.019).This investigation suggested that males and individuals with a higher educational background are more likely to possess optimistic perspectives concerning the impacts of ecotourism.Furthermore,individuals residing in districts relatively near ecotourism destinations,those engaged in tourism-related work,and younger participants were recognized as having optimistic viewpoints regarding the impacts of ecotourism.In conclusion,the study highlighted the need for collaborative efforts among stakeholders to maximize ecotourism’s benefits and minimize negative effects in SMNP.To achieve this,it is recommended that the Ethiopian Wildlife Conservation Agency,the Ministry of Tourism,and local government should work together to develop sustainable tourism plans that enhance local livelihoods and park conditions.Efforts should focus on increasing the participation of females and individuals with lower education levels through training and resource provision.展开更多
Abstract Objective To conduct a screening survey of glycophorin (GP) variants and observe the content changes of sialic acid (SA) and total sulfhydryl (SH) groups on the erythrocyte membranes among residents in a...Abstract Objective To conduct a screening survey of glycophorin (GP) variants and observe the content changes of sialic acid (SA) and total sulfhydryl (SH) groups on the erythrocyte membranes among residents in a tertian malaria hyperendemic area of Guizhou Province. Methods GP variants were detected in the erythrocyte hemolysates of 173 local residents at two villages of Libo County by SDS PAGE on 10% to 15% gradients gel and Western immunoblotting. Their SA and total SH group contents were estimated in erythrocyte membranes by spectrophotometric methods. 114 healthy subjects in Changsha and 49 individuals at a neighbouring village of the above area showing low morbidity of malaria served as normal and endemic controls respectively. Results Three distinct types of GP variants were found among 19 propositi in this hyperendemic area. The incidence of GP variants was 7.9% (8/101) at Yaolu Village whose population was mainly composed of Yao ethnic group;while that of Buyi ethnic group at Maolan Village was higher (15.3%; 11/72).The erythrocyte membrane contents of SA in residents at both villages exhibited a very significant tendency of decline (P<0.01), whereas those of total SH groups increased prominently in residents of Yaolu Village only (P<0.05). Conclusions The frequency of GP variants in this hyperendemic area does not depend upon the severity of malarial prevalence. The evident reduction of SA contents in the residents may be related to the breaking down of the SA residues on membrane GPs by the invasion of Plasmodium vivax.展开更多
Star River(or Star Bay),an elite works of Hong Yu Group,is located in Sijixinghe Road,Chaoyang District,Beijing.It is a distinguished residence area,occupying land of 300 thousand sq.meters.The north side is a forest ...Star River(or Star Bay),an elite works of Hong Yu Group,is located in Sijixinghe Road,Chaoyang District,Beijing.It is a distinguished residence area,occupying land of 300 thousand sq.meters.The north side is a forest garden with 1600 or more mu,the eastern side is a green belt with more than 2000 mu.展开更多
基金supported by the 12th Five-Year Major National Science and Technology Projects of China (No. 2012ZX10004219-007) and ( No. 2013ZX10004001)
文摘Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay （IFA）. Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces （the exception being the Miyun area in Beijing）. The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces.
文摘This study aims to analyze how park households perceive the economic,sociocultural,and environmental impacts of ecotourism in Simien Mountain National Park(SMNP),Ethiopia.We combined qualitative and quantitative techniques for data collection.A sequentially embedded mixed design that gives priority to quantitative data was used.We also employed a multistage sampling technique to select respondents from three districts or woredas,namely Debark,Janamora,and Beyeda woredas.Finally,397 participants were involved in the study from a random selection of three villages from each district or woreda.Data were gathered using a survey questionnaire,interviews,and focus group discussions.Data were analyzed using both descriptive and inferential statistical methods,including means,standard deviations,analysis of variance(ANOVA),and logistic regression models.The findings from a logistic regression analysis revealed that ecotourism participation had a significant relationship with gender(B=1.850,P=0.023),level of education(B=0.238,P=0.032),geographical location(B=0.420,P=0.041),and occupation(B=0.920,P=0.019).This investigation suggested that males and individuals with a higher educational background are more likely to possess optimistic perspectives concerning the impacts of ecotourism.Furthermore,individuals residing in districts relatively near ecotourism destinations,those engaged in tourism-related work,and younger participants were recognized as having optimistic viewpoints regarding the impacts of ecotourism.In conclusion,the study highlighted the need for collaborative efforts among stakeholders to maximize ecotourism’s benefits and minimize negative effects in SMNP.To achieve this,it is recommended that the Ethiopian Wildlife Conservation Agency,the Ministry of Tourism,and local government should work together to develop sustainable tourism plans that enhance local livelihoods and park conditions.Efforts should focus on increasing the participation of females and individuals with lower education levels through training and resource provision.
文摘Abstract Objective To conduct a screening survey of glycophorin (GP) variants and observe the content changes of sialic acid (SA) and total sulfhydryl (SH) groups on the erythrocyte membranes among residents in a tertian malaria hyperendemic area of Guizhou Province. Methods GP variants were detected in the erythrocyte hemolysates of 173 local residents at two villages of Libo County by SDS PAGE on 10% to 15% gradients gel and Western immunoblotting. Their SA and total SH group contents were estimated in erythrocyte membranes by spectrophotometric methods. 114 healthy subjects in Changsha and 49 individuals at a neighbouring village of the above area showing low morbidity of malaria served as normal and endemic controls respectively. Results Three distinct types of GP variants were found among 19 propositi in this hyperendemic area. The incidence of GP variants was 7.9% (8/101) at Yaolu Village whose population was mainly composed of Yao ethnic group;while that of Buyi ethnic group at Maolan Village was higher (15.3%; 11/72).The erythrocyte membrane contents of SA in residents at both villages exhibited a very significant tendency of decline (P<0.01), whereas those of total SH groups increased prominently in residents of Yaolu Village only (P<0.05). Conclusions The frequency of GP variants in this hyperendemic area does not depend upon the severity of malarial prevalence. The evident reduction of SA contents in the residents may be related to the breaking down of the SA residues on membrane GPs by the invasion of Plasmodium vivax.
文摘Star River(or Star Bay),an elite works of Hong Yu Group,is located in Sijixinghe Road,Chaoyang District,Beijing.It is a distinguished residence area,occupying land of 300 thousand sq.meters.The north side is a forest garden with 1600 or more mu,the eastern side is a green belt with more than 2000 mu.
