Ecological effects of crude oil residues on the functional diversity of soil microorganisms in three weed rhizospheres

Ecological effects of crude oil residues on weed rhizospheres are still vague. The quantitative and diversity changes and metabolic responses of soil-bacterial communities in common dandelion （Taraxacum officinale）, jerusalem artichoke （Silphiurn perfoliatum L.） and evening primrose （A colypha australis L.） rhizospheric soils were thus examined using the method of carbon source utilization. The results indicated that there were various toxic effects of crude oil residues on the growth and reproduction of soil bacteria, but the weed rhizospheres could mitigate the toxic effects. Total heterotrophic counting colony-forming units （CFUs） in the rhizospheric soils were significantly higher than those in the non-rhizospheric soils. The culturable soil-bacterial CFUs in the jerusalem artichoke （S. perfoliatum） rhizosphere polluted with 0.50 kg/pot of crude oil residues were almost twice as much as those with 0.25 kg/pot and without the addition of crude oil residues. The addition of crude oil residues increased the difference in substrate evenness, substrate richness, and substrate diversity between non-rhizospheric and rhizospheric soils of T. officinale and A. australis, but there was no significant （p〉0.05） difference in the Shannon＇s diversity index between non-rhizospheric and rhizospheric soils of S. perfoliatum. The rhizospheric response of weed species to crude oil residues suggested that S. perfoliatum may be a potential weed species for the effective plant-microorganism bioremediation of contaminated soils by crude oil residues.

Utilization mechanism of foam flooding and distribution situation of residual oil in fractured-vuggy carbonate reservoirs

The development of fractured-vuggy carbonate reservoirs is extremely difficult because of the complex fractured-vuggy structure and strong heterogeneity.Foam flooding is a potential enhanced oil recovery(EOR)technology in fractured-vuggy carbonate reservoirs.Based on the similarity criterion,three types of 2D visual physical models of the fractured-vuggy structure were made by laser ablation technique,and a 3D visual physical model of the fractured-vuggy reservoir was made by 3D printing technology.Then the physical analog experiments of foam flooding were carried out in these models.The experimental results show that foam can effectively improve the mobility ratio,control the flow velocity of the fluid in different directions,and sweep complex fracture networks.The effect of foam flooding in fractures can be improved by increasing foam strength and enhancing foam stability.The effect of foam flooding in vugs can be improved by reducing the density of the foam and the interfacial tension between foam and oil.Three types of microscopic residual oil and three types of macroscopic residual oil can be displaced by foam flooding.This study verifies the EOR of foam flooding in the fractured-vuggy reservoir and provides theoretical support for the application of foam flooding in fractured-vuggy reservoirs.

Effect of petroleum chemical fraction and residual oil content in saline lacustrine organic-rich shale: A case study from the Paleogene Dongpu Depression of North China

Halite and gypsum minerals in saline shale make the retention mechanism and chemical fractionation of residual oil unique. The Dongpu Depression in North China is a typically saline lacustrine basin with developing halite and gypsum. The effect of gypsum minerals on residual oil content and chemical fractionation remains unclear. In this study, shale samples with different gypsum contents were used in organic geochemical experiments, showing that the high total organic matter (TOC) content and type II kerogen leads to a high residual oil content, as shown by high values of volatile hydrocarbon (S1) and extractable organic matter (EOM). XRD and FE-SEM result indicate that the existence of gypsum in saline shale contributes to an enhanced pore space and a higher residual oil content in comparison to non-gypsum shale. Additionally, the increase in the gypsum mineral content leads to an increase in the saturated hydrocarbon percentage and a decrease in polar components percentage (resins and asphaltene). Furthermore, thermal simulation experiments on low-mature saline shale show that the percentage of saturated hydrocarbons in the residual oil is high and remains stable and that the storage space is mainly mesoporous (> 20 nm) in the oil expulsion stage. However, the saturated hydrocarbons percentage decreases rapidly, and oil exists in mesopores (> 20 nm and < 5 nm) in the gas expulsion stage. In general, gypsum is conducive to the development of pore space, the adsorption of hydrocarbons and the occurrence of saturated hydrocarbon, leading to large quantities of residual oil. The data in this paper should prove to be reliable for shale oil exploration in saline lacustrine basins.

Numerical analysis of water-alternating-CO_(2) flooding for CO_(2)-EOR and storage projects in residual oil zones

Residual oil zones(ROZs)have high residual oil saturation,which can be produced using CO_(2) miscible flooding.At the same time,these zones are good candidates for CO_(2) sequestration.To evaluate the coupled CO_(2)-EOR and storage perfor-mance in ROZs for Water-Alternating-CO_(2)(WAG)flooding,a multi-compositional CO_(2) miscible model with molecular diffusion was developed.The effects of formation parameters(porosity,permeability,temperature),operation parameters(bottom hole pressure,WAG ratio,pore volume of injected water),and diffusion coeffcient on the coupled CO_(2)-EOR and storage were investigated.Five points from the CO_(2) sequestration curve and the oil recovery factor curve were selected to help better analyze coupled CO_(2)-EOR and storage.The results demonstrate that enhanced performance is observed when formation permeability is higher and a larger volume of water is injected.On the other hand,the performance diminishes with increasing porosity,molecular diffusion of gas,and the WAG ratio.When the temperature is around 100℃,coupled CO_(2)-EOR and storage performance is the worst.To achieve optimal miscible flooding,it is recommended to maintain the bottom hole pressure(BHP)of the injection well above 1.2 minimum miscibility pressure(MMP),while ensuring that the BHP of the production well remains sufficiently high.Furthermore,the tapered WAG flooding strategy proves to be profitable for enhanced oil recovery,as compared to a WAG ratio of 0.5:1,although it may not be as effective for CO_(2) sequestration.

Reservoir heterogeneity controls of CO_(2)-EOR and storage potentials in residual oil zones:Insights from numerical simulations

Residual oil zones(ROZs)have large potential for CO_(2)enhanced oil recovery(EOR)and geologic storage.During CO_(2)injection,the migration of CO_(2)in ROZs controls the performance of both EOR and storage.However,it has not been clearly visualized and understood that how geological heterogeneity factors control the transport of CO_(2)in ROZs.In this study,the oil recovery performance and geologic storage potential during continuous CO_(2)injection in a representative ROZ are studied based on geostatistical modelling and high-fidelity three-phase flow simulation.We examined the influence of autocorrelation length of permeability,global heterogeneity(DykstraeParsons coefficient),and permeability anisotropy on cumulative oil recovery and CO_(2)retention fraction.Simulation results indicate that,as the permeability autocorrelation length increases,the cumulative oil recovery and CO_(2)storage efficiency decrease.This results from the accelerated migration of CO_(2)along high permeability zones(i.e.,gas channeling).The increase in global heterogeneity and permeability anisotropies can lead to low oil recovery and poor CO_(2)sequestration performance,depending on the degree of CO_(2)channeling.The net utilization ratio of CO_(2)(CO_(2)retained/oil produced)unfavorably increases with both autocorrelation length and Dykstra eParsons coefficient,but decreases with the increase in kv/kh.Such a decrease is attributed to enlarged swept volume induced by gravity override.The study provides important implications for fieldscale CO_(2)EOR and storage applications in ROZs.

Stabilization of sand dunes with oil residue: Application to civil engineering construction and environmental implications

The present work ascertains the feasibility of oil residue treatment for stabilizing wind-blown sand dunes. Various combinations of natural collapsible saline from the Jandaq desert of Iran and oil residue from distillation towers of Iranian refineries were tested in laboratory experiments. Stabilized sands were evaluated in terms of geotechnical properties, permeability, and oil retention characteristics(i.e. bonding mechanisms, leaching and migrating behaviour of oil residue from the stabilized sands). Since the presence of oil residue in soils can pose an environmental threat, the optimum retention capacity of the stabilized sands is of critical concern. Relative to sand that was not augmented with oil residue, specimens made of 7% oil residues had the highest compressive strength, significantly higher cohesion and load bearing capacity, and considerably lower permeability. The effect of distilled water, saline water and municipal sewage on prepared specimens were also evaluated.

CO_(2) gas stripped off membranous residual oil from pore surfaces: Effects of temperature, pressure and wettability

The threshold values of CO_(2) gas stripped off membranous residual oil from the pore walls are not clear under different temperatures, pressures and wettability conditions. The extent to which temperature, pressure and wettability influence CO_(2) flooding for enhancing the recovery of residual oil in membranous formations also remains uncertain. Therefore, further quantitative characterization is entailed. In this study, the molecular dynamics method was employed to explore CO_(2) flooding under different temperatures, pressures and wettability conditions, aiming to enhance the production of membranous residual oil. The results reveal that the interaction energy between CO_(2), decane molecules and pore walls exhibits a decrease with increasing temperature and an increase with increasing pressure, respectively, in distinct wettability scenarios. When the temperature was at or below 363 K and the pressure was not lower than 40 MPa, CO_(2) gas could detach the membranous residual oil from the pore walls in the water-wet systems. When the temperature was equal to 363 K and the pressure remained under 40 MPa, or the temperature surpassed 363 K, CO_(2) gas failed to detach the membranous residual oil from the pore walls in the water-wet systems. For the mixed-wet and oil-wet systems, CO_(2) molecules could not detach the membranous residual oil from the pore walls. The hierarchy of influence regarding temperature, pressure and wettability on the competitive adsorption capacity of CO_(2) and decane molecules on the pore walls emerged as follows: wettability > temperature > pressure. The findings of this study offer valuable insights into the application of CO_(2) gas flooding for the exploitation of membranous residual oil on pore walls.

Residual oil evolution based on displacement characteristic curve

The purpose of this study was to determine the displacement and dynamic distribution characteristics of the remaining oil in the two development stages of water flooding and subsequent alkaline surfactant polymer(ASP) flooding. The well pattern types in the water and ASP flooding stages are a longdistance determinant well pattern and short-distance five-point well pattern, respectively. The type A displacement characteristic curve can be obtained using the production data, and the slope of the straight-line section of the curve can reflect the displacement strength of the oil displacement agent. A numerical simulation was carried out based on the geological model. The results revealed that the injected water advances steadily with a large-distance determinant water-flooding well pattern. The single-well water production rate increases monotonically during water flooding. There is a significant positive correlation between the cumulative water-oil ratio and the formation parameter. Differential seepage between the oil and water phases is the main factor causing residual oil formation after water flooding, while the residual oil is still relatively concentrated. The effect of the chemical oildisplacement agent on improving the oil-water two-phase seepage flow has distinct stages during ASP flooding. The remaining oil production is extremely sporadic after ASP flooding.

Study on the Effect of Catalyst Properties on Residue Hydroconversion

The effect of catalyst properties on residue oil hydroconversion was studied at moderate operating conditions(at a temperature of 400 ℃, an initial hydrogen pressure of 10 MPa, and a reaction time of 4 h) in a batch mode slurry phase with different catalyst samples. The results showed that the catalyst acidity had a good effect on residue conversion and MCR(micro carbon residue) conversion but brought about higher coke yield. Residue conversion was thermally induced but the catalyst acidity changed its conversion route. A catalyst with higher metal loading, higher hydrogenation activity and appropriate pore size had higher sulfur and metal removal rate, higher MCR conversion and also a lower coke formation. The activity of spent commercial catalyst AS1 and DS1 was slightly lower than the corresponding fresh ones but was still high enough for residue oil hydroconversion. It assumes that the role of the catalyst is to activate hydrogen species toward reaction with an aromatic carbon radical to yield a cyclohexadienyl type intermediate which will turn into liquid and also to absorb the mesophase which can easily aggregate to form coke.

A model study of residual oil distribution jointly using crosswell and borehole-surface electric potential methods

Although high resolution can be provided by electrical logging, the measured electrical log range is narrow and is limited to near the well. Borehole-surface electric potential measurements are able to detect a wide enough range but its resolution is limited, particularly for reservoirs with complex oil and water distribution or complicated structure. In this study, we attempt to accurately locate the 3-D reservoir water and oil distribution by combining borehole-surface and crosswell electric potentials. First, the distributions of oil and water in both vertical and horizontal directions are detected by the borehole-surface and erosswell electric potential methods, respectively, and then the measured crosswell potential result is used to calibrate the measured borehole-surface electric potential data to improve vertical resolution so that the residual oil distribution is determined in a lower half-space with three dimensions. The evaluation of residual oil distribution is obtained by investigation of differences between the simulation results of the reservoir with and without water flooding. The finite difference numerical simulation results prove that the spatial residual oil distribution can be effectively determined by combining the crosswell and borehole-surface electric potentials.

Micro-mechanisms of residual oil mobilization by viscoelastic fluids

Four typical types of residual oil, residual oil trapped in dead ends, oil ganglia in pore throats, oil at pore corners and oil film adhered to pore walls, were studied. According to main pore structure characteristics and the fundamental morphological features of residual oil, four displacement models for residual oil were proposed, in which pore-scale flow behavior of viscoelastic fluid was analyzed by a numerical method and micro-mechanisms for mobilization of residual oil were discussed. Calculated results indicate that the viscoelastic effect enhances micro displacement efficiency and increases swept volume. For residual oil trapped in dead ends, the flow field of viscoelastic fluid is developed in dead ends more deeply, resulting in more contact with oil by the displacing fluid, and consequently increasing swept volume. In addition, intense viscoelastic vortex has great stress, under which residual oil becomes small oil ganglia, and finally be carried into main channels. For residual oil at pore throats, its displacement mechanisms are similar to the oil trapped in dead ends. Vortices are developed in the depths of the throats and oil ganglia become smaller. Besides, viscoelastic fluid causes higher pressure drop on oil ganglia, as a driving force, which can overcome capillary force, consequently, flow direction can be changed and the displacing fluid enter smaller throats. For oil at pore corners, viscoelastic fluid can enhance displacement efficiency as a result of greater velocity and stress near the corners. For residual oil adhered to pore wall, viscoelastic fluid can provide a greater displacing force on the interface between viscoelastic fluid and oil, thus, making it easier to exceed the minimum interfacial tension for mobilizing the oil film.

Degradation Kinetics of Petroleum Contaminants in Soil-Water Systems

On the basis of site investigation and sample collection of petroleum contaminants in the soil-water-crop system in the Shenyang-Fushun sewage irrigation area, the physical-chemical-biological compositions of the unsaturated zone is analyzed systematically in this paper. At the same time, the degradation kinetics of residual and aqueous oils is determined through biodegradation tests. The studies show that dominant microorganisms have been formed in the soils after long-term sewage irrigation. The microorganisms mainly include bacteria, and a few of fungus and actinomycetes. After a 110-days' biodegradation test, the degradation rate of residual oil is 9.74%-10.63%, while the degradation rate of aqueous oil reaches 62.43%. This indicates that the degradation rate of low-carbon aqueous oil is higher than that of high-carbon residual oil. In addition, although microbial degradation of petroleum contaminants in soils is suitable to the first-order kinetics equation, the half-lives of aqueous oil, No. 20 heavy diesel and residual oil in the surface soils (L2-1, S1-1 and X1-1) are 1732 h, 3465 h and 17325 h, respectively.

Factors influencing oil recovery by surfactant-polymer flooding in conglomerate reservoirs and its quantitative calculation method

This study aims to clarify the factors influencing oil recovery of surfactant-polymer(SP)flooding and to establish a quantitative calculation model of oil recovery during different displacement stages from water flooding to SP flooding.The conglomerate reservoir of the Badaowan Formation in the seventh block of the Karamay Oilfield is selected as the research object to reveal the start-up mechanism of residual oil and determine the controlling factors of oil recovery through SP flooding experiments of natural cores and microetching models.The experimental results are used to identify four types of residual oil after water flooding in this conglomerate reservoir with a complex pore structure:oil droplets retained in pore throats by capillary forces,oil cluster trapped at the junction of pores and throats,oil film on the rock surface,isolated oil in dead-ends of flow channel.For the four types of residual oil identified,the SP solution can enhance oil recovery by enlarging the sweep volume and improving the oil displacement efficiency.First,the viscosity-increasing effect of the polymer can effectively reduce the permeability of the displacement liquid phase,change the oil-water mobility ratio,and increase the water absorption.Furthermore,the stronger the shear drag force of the SP solution,the more the crude oil in a porous medium is displaced.Second,the surfactant can change the rock wettability and reduce the absorption capacity of residual oil by lowering interfacial tension.At the same time,the emulsification further increases the viscosity of the SP solution,and the residual oil is recovered effectively under the combined effect of the above two factors.For the four start-up mechanisms of residual oil identified after water flooding,enlarging the sweep volume and improving the oil displacement efficiency are interdependent,but their contribution to enhanced oil recovery are different.The SP flooding system primarily enlarges the sweep volume by increasing viscosity of solution to start two kinds of residual oil such as oil droplet retained in pore throats and isolated oil in dead-ends of flow channel,and primarily improves the oil displacement efficiency by lowing interfacial tension of oil phase to start two kinds of residual oil such as oil cluster trapped at the junction of pores and oil film on the rock surface.On this basis,the experimental results of the oil displacement from seven natural cores show that the pore structure of the reservoir is the main factor influencing water flooding recovery,while the physical properties and original oil saturation have relatively little influence.The main factor influencing SP flooding recovery is the physical and chemical properties of the solution itself,which primarily control the interfacial tension and solution viscosity in the reservoir.The residual oil saturation after water flooding is the material basis of SP flooding,and it is the second-most dominant factor controlling oil recovery.Combined with the analysis results of the influencing factors and reservoir parameters,the water flooding recovery index and SP flooding recovery index are defined to further establish quantitative calculation models of oil recovery under different displacement modes.The average relative errors of the two models are 4.4%and 2.5%,respectively;thus,they can accurately predict the oil recovery of different displacement stages and the ultimate reservoir oil recovery.

Experimental study on the oil production characteristics during the waterflooding of different types of reservoirs in Ordos Basin, NW China

Waterflooding experiments were conducted in micro-models(microscopic scale)and on plunger cores from low permeability,extra-low permeability and ultra-low permeability reservoirs in the Ordos Basin under different displacement pressures using the NMR techniques to find out pore-scale oil occurrence state,oil production characteristics and residual oil distribution during the process of waterflooding and analyze the effect of pore structure and displacement pressure on waterflooding efficiency.Under bound water condition,crude oil mainly occurs in medium and large pores in the low-permeability sample,while small pores and medium pores are the main distribution space of crude oil in extra-low permeability and ultra-low permeability samples.During the waterflooding,crude oil in the medium and large pores of the three types of samples are preferentially produced.With the decrease of permeability of the samples,the waterflooding front sequentially shows uniform displacement,network displacement and finger displacement,and correspondingly the oil recovery factors decrease successively.After waterflooding,the residual oil in low-permeability samples is mainly distributed in medium pores,and appears in membranous and angular dispersed phase;but that in the extra-low and ultra-low permeability samples is mainly distributed in small pores,and appears in continuous phase formed by a bypass flow and dispersed phase.The low-permeability samples have higher and stable oil displacement efficiency,while the oil displacement efficiency of the extra-low permeability and ultra-low permeability samples is lower,but increases to a certain extent with the increase of displacement pressure.

Effects of Fe^(2+), Co^(2+) and Ni^(2+) Ions on Biological Methane Production from Residual Heavy Oil

On the basis of single factor tests, the effect of trace elements—Fe2+, Co2+ and Ni2+ ions—on biological methane production from heavy oil was investigated by the response surface method. A three-level Box-Behnken design was employed to study the relationship between the independent variables and the dependent variable by applying initial Fe2+, Co2+ and Ni2+ concentration as the independent variables(factors) and using the methane production after 270 days of cultivation as the dependent variable(response). A prediction model of quadramatic polynomial regression equation was obtained. The results showed that the methane production could be as high as 240.69 μmol after optimization compared with 235.74 μmol obtained under un-optimized condition. Furthermore, the microbial communities before and after biodegradation were analyzed by PCR-DGGE method. The dominant bands were recovered and sequenced. Three strains were obtained; the strain T1 has 97% similarity with Bacillus thermoamylovorans, the strain H3 has 97% similarity with Bacillus thermoamylovorans and the strain H4 has 99% similarity with Bacillus vietnamensis.

Theoretical analysis of microscopic oil displacement mechanism by viscoelastic polymer solution

The microscopic oil displacement mechanism in viscoelastic polymer flooding is theoretically analyzed with mechanical method.The effects of viscoelasticity of polymer solution on such three kinds of residual oil as in pore throat,in sudden expansion pore path,and in dead end are analyzed.Results show that the critical radius of mobile residual oil for viscoelastic polymer solution is larger than that for viscous polymer solution,which makes the oil that is immobile in viscous polymer flooding displaced under the condition of viscoelastic polymer solution.The viscous polymer solution hardly displaces the oil in dead ends.However,when the effect of viscoelasticity is considered,the residual oil in sudden expansion pore paths and dead ends can be partly displaced. A dimensionless parameter is suggested to denote the relative dominance of gravity and capillary pressure.The larger the dimensionless parameter,the more accurate the increment expressions.

Residue Upgrading in Slurry Phase over Ultra-fine NiMo/γ-Al_2O_3 Catalyst

In this article, residual oil hydroconversion was studied in slurry phase in the presence of fine solid Ni Mo/γ-Al2O3 catalyst and the effects of operating conditions were carefully studied. The results showed that residue conversion was only affected by the reaction temperature and reaction time. The coke yield increased with a higher reaction temperature, a bigger catalyst particle size, a longer reaction time, a lower initial hydrogen pressure and a lower catalyst concentration. Heteroatoms removal rate increased with a higher reaction temperature, a longer reaction time, a higher initial hydrogen pressure, a higher catalyst concentration, and a smaller catalyst particle size. The role of catalyst in the slurry bed technology was discussed and its function could be stated as follows: the metal was applied to activate the hydrogen atoms for removing heteroatoms and saturating aromatics, while the support of the catalyst was used to prevent the mesophase coalescence for reducing coke formation.

Study on Residual Oil HDS Process with Mechanism Model and ANN Model

Based on the Residual Oil Hydrodesulfurization Treatment Unit (S-RHT), the n-order reaction kinetic model for residual oil HDS reactions and artificial neural network (ANN) model were developed to determine the sulfur content of hydrogenated residual oil. The established ANN model covered 4 input variables, 1 output variable and 1 hidden layer with 15 neurons. The comparison between the results of two models was listed. The results showed that the predicted mean relative errors of the two models with three different sample data were less than 5% and both the two models had good predictive precision and extrapolative feature for the HDS process. The mean relative error of 5 sets of testing data of the ANN model was 1.62%—3.23%, all of which were smaller than that of the common mechanism model (3.47%— 4.13%). It showed that the ANN model was better than the mechanism model both in terms of fitting results and fitting difficulty. The models could be easily applied in practice and could also provide a reference for the further research of residual oil HDS process.

Application of Tracer Model in the Remaining Oil Distribution of 95 Block Oilfields of Central Plains

With the development and utilization of oil and gas fields, oil mining become more and more difficult. The remaining re- serves of oil in the oil distribution is difficult to confirm, in order to understand and grasp the distribution of the remaining oil in the reser-voir, calculated using tracer concentration methods used mathematical models to calculate the residual oil saturation in the reservoir, from the theoretical analysis of tracer migration process, you can more accurately determine the distribution of the remaining oil, this method has good prospects for development.

Research on Influence of Micro Structure on Residual Oil Distribution

Researching residual oil distribution not only is a difficulty in the world, but also is the pioneering research subject in different fields such as geology, physical geography and reservoir engineering. The modem geology technique, well logging technology and reservoir engineering technique develops rapidly, which provides favorable conditions for researching residual oil distribution.