Background:Resina Draconis is a traditional Chinese medicine mainly used to treat pain.However,the pharmacological mechanisms and chemical composition of Resina Draconis are not clear yet.Methods:In this study,based o...Background:Resina Draconis is a traditional Chinese medicine mainly used to treat pain.However,the pharmacological mechanisms and chemical composition of Resina Draconis are not clear yet.Methods:In this study,based on the 21 main active components of Resina Draconis previously analyzed by our group,the potential action targets of the active components were predicted and screened out by using the databases such as Swiss Target Prediction and Pharmapper.The genes corresponding to the related targets were retrieved by UniProt and GeneCards,and then the"component-target"network model was established using Cytoscape 3.9.1 software.The protein-protein interaction network was constructed by using STRING database for analysis.The STRING database was used for enrichment analysis of gene ontology and Kyoto Encyclopedia of Genes and Genome pathways to explore the underlying action mechanisms.Result:A total of 21 main analgesic active components of Resina Draconis and 77 intersecting targets of Resina Draconis and pain were screened out.PPI network analysis indicated that such targets as albumin(ALB),tumor necrosis factor(TNF),RAC-alpha serine/threonine-protein kinase 1(AKT1)and epidermal growth factor receptor(EGFR)might be the core targets of analgesia.Through gene ontology enrichment analysis,a total of 169 gene ontology entries were obtained(P<0.01),including 111 biological processes,31 molecular functions and 27 cellular components.Through enrichment analysis of KEGG pathways,a total of 112(P<0.01)signaling pathways were screened.Conclusion:Dracaenogenins A,Resveratrol and 7,4′-dihydroxyflavone in Resina Draconis may be the main material basis for analgesia,which can interact with multiple targets such as AlB,AKT1,TNF,and EGFR,and exerts analgesic effect through signaling pathways such as mitogen-activated protein kinase(MAPK)signaling pathway,PI3K-Akt signaling pathway,and Rap1 signaling pathway.展开更多
“龙血竭”是龙血树属(Dracaena)植物树干产生的红色树脂,被称为“云南红药”。国药批准的国产“龙血竭”基原植物为天门冬科的柬埔寨龙血树(D.cambodianaPierre ex Gagnep.)和剑叶龙血树(D.cochinchinensis(Lour.)S.C.Chen)。由于文献...“龙血竭”是龙血树属(Dracaena)植物树干产生的红色树脂,被称为“云南红药”。国药批准的国产“龙血竭”基原植物为天门冬科的柬埔寨龙血树(D.cambodianaPierre ex Gagnep.)和剑叶龙血树(D.cochinchinensis(Lour.)S.C.Chen)。由于文献对两种龙血树的界定不清,在其名称上存在混淆使用。本研究对两种龙血树及其混淆种进行原始文献和模式标本考证,并利用龙血树属19种33个质体基因组数据开展系统发育重建。结果显示,19种龙血树可分成3个分支,其中,产龙血竭的物种位于分支Ⅰ和分支Ⅲ,柬埔寨龙血树样品分成两个独立的分支,即海南分支和云南分支。基于原始文献和模式标本考证,确认剑叶龙血树的形态与长花龙血树(D.angustifolia(Medik.)Roxb.)基本一致,可能是长花龙血树的一个新异名。本文结合龙血树质体基因组的系统发育和形态证据,明确了柬埔寨龙血树是蔡希陶等最早发现的国产龙血竭的基原植物,并确认岩棕(D.saposchnikowii Regel)和广西龙血树(未知种2)也是国产龙血竭的资源植物。展开更多
基金supported by National Natural Science Foundation of China(No.82074137)Guangdong Basic and Applied Basic Research Foundation(No.2020A1515011515)Guangdong Undergraduate Innovation and Entrepreneurship Training Program(No.S202210573050).
文摘Background:Resina Draconis is a traditional Chinese medicine mainly used to treat pain.However,the pharmacological mechanisms and chemical composition of Resina Draconis are not clear yet.Methods:In this study,based on the 21 main active components of Resina Draconis previously analyzed by our group,the potential action targets of the active components were predicted and screened out by using the databases such as Swiss Target Prediction and Pharmapper.The genes corresponding to the related targets were retrieved by UniProt and GeneCards,and then the"component-target"network model was established using Cytoscape 3.9.1 software.The protein-protein interaction network was constructed by using STRING database for analysis.The STRING database was used for enrichment analysis of gene ontology and Kyoto Encyclopedia of Genes and Genome pathways to explore the underlying action mechanisms.Result:A total of 21 main analgesic active components of Resina Draconis and 77 intersecting targets of Resina Draconis and pain were screened out.PPI network analysis indicated that such targets as albumin(ALB),tumor necrosis factor(TNF),RAC-alpha serine/threonine-protein kinase 1(AKT1)and epidermal growth factor receptor(EGFR)might be the core targets of analgesia.Through gene ontology enrichment analysis,a total of 169 gene ontology entries were obtained(P<0.01),including 111 biological processes,31 molecular functions and 27 cellular components.Through enrichment analysis of KEGG pathways,a total of 112(P<0.01)signaling pathways were screened.Conclusion:Dracaenogenins A,Resveratrol and 7,4′-dihydroxyflavone in Resina Draconis may be the main material basis for analgesia,which can interact with multiple targets such as AlB,AKT1,TNF,and EGFR,and exerts analgesic effect through signaling pathways such as mitogen-activated protein kinase(MAPK)signaling pathway,PI3K-Akt signaling pathway,and Rap1 signaling pathway.
文摘“龙血竭”是龙血树属(Dracaena)植物树干产生的红色树脂,被称为“云南红药”。国药批准的国产“龙血竭”基原植物为天门冬科的柬埔寨龙血树(D.cambodianaPierre ex Gagnep.)和剑叶龙血树(D.cochinchinensis(Lour.)S.C.Chen)。由于文献对两种龙血树的界定不清,在其名称上存在混淆使用。本研究对两种龙血树及其混淆种进行原始文献和模式标本考证,并利用龙血树属19种33个质体基因组数据开展系统发育重建。结果显示,19种龙血树可分成3个分支,其中,产龙血竭的物种位于分支Ⅰ和分支Ⅲ,柬埔寨龙血树样品分成两个独立的分支,即海南分支和云南分支。基于原始文献和模式标本考证,确认剑叶龙血树的形态与长花龙血树(D.angustifolia(Medik.)Roxb.)基本一致,可能是长花龙血树的一个新异名。本文结合龙血树质体基因组的系统发育和形态证据,明确了柬埔寨龙血树是蔡希陶等最早发现的国产龙血竭的基原植物,并确认岩棕(D.saposchnikowii Regel)和广西龙血树(未知种2)也是国产龙血竭的资源植物。
