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Antimicrobial Resistance,Virulence Profile,and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China,2013-2014 被引量:3
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作者 YAN Shao Fei WANG Wei +4 位作者 BAI Li HU Yu Jie DONG Yin Ping XU Jin LI Feng Qin 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2016年第6期448-452,共5页
We aimed to investigate the potential pathogenic profile and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat food in China.Antimicrobial resistance was determined by broth microdilution foll... We aimed to investigate the potential pathogenic profile and antibiotic resistance of Listeria monocytogenes isolated from ready-to-eat food in China.Antimicrobial resistance was determined by broth microdilution following the Clinical and Laboratory Standards Institute protocol Molecular serotyping,virulence,and resistance genes were identified using PCR.Multi-locus 展开更多
关键词 MLST gene Antimicrobial resistance Virulence profile and Molecular Characterization of Listeria monocytogenes Isolated from Ready-to-eat Food in China 2013-2014 SMZ CLSI
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Phenotipic Prevalence of Antibiotic Resistance in Gabon
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作者 Annicet-Clotaire Dikoumba Richard Onanga +8 位作者 Pierre Philippe Mbehang Nguema Laurette G. Mangouka Berthe Amélie Iroungou Fabrice Kassa Kassa Berthold Bivigou Mboumba Elvire Mbongo Kama Jean-Fabrice Yala Edgard-Brice Ngoungou Sylvain Godreuil 《Open Journal of Medical Microbiology》 2021年第2期100-118,共19页
<b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> The increasing phenome... <b><span style="font-family:Verdana;">Background:</span></b><span style="font-family:""><span style="font-family:Verdana;"> The increasing phenomenon of bacterial resistance to antibiotics is a real public health problem. The main causes are poor management of hygiene and water quality, but also the use of antibiotics without precaution. The objective of this study was to isolate and determine the antibiotic resistance profile of the different bacteria found in the main hospitals and bacteriology laboratories in Gabon. </span><b><span style="font-family:Verdana;">Methods:</span></b><span style="font-family:Verdana;"> 6034 samples were taken from hospitals in seven main cities of Gabon, and analyzed according to the usual techniques. The pathogenic strains were identified by Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometry. Antimicrobial susceptibility testing was performed by the agar disc diffusion method, according to the Antibiogram Committee of the French Society for Microbiology guidelines. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">974 pathogenic bacterial strains were found, including 890/974 (91</span><span style="font-family:Verdana;">.4%) Gram-negative bacilli. The systematic antimicrobial suscepti</span><span style="font-family:Verdana;">bility testings identified 160/974 (16.4%) multi-resistant strains. </span><i><span style="font-family:Verdana;">Escherichia coli</span></i><span style="font-family:Verdana;"> was t</span><span style="font-family:Verdana;">he most represented species. 12.5%</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">25% of </span><i><span style="font-family:Verdana;">Escherichia coli</span></i><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Klebsiel</span></i></span><i><span style="font-family:Verdana;">la pneumoniae</span></i><span style="font-family:""><span style="font-family:Verdana;">, </span><i><span style="font-family:Verdana;">Enterobacter cloacae</span></i><span style="font-family:Verdana;">, and </span><i><span style="font-family:Verdana;">Citrobacter sedlakii</span></i><span style="font-family:Verdana;"> strains were resistant to amoxicillin + clavulanic acid, third and fourth generation cephalosporins. Aminoglycoside resistance rates of 8.5%</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">19% were also noted. 4.5% to 25% of the bacteria found were resistant to quinolones and cotrimoxazole. Resistance rates to carbapenems ranged from 1% to 10.5%. 16% of </span><i><span style="font-family:Verdana;">Staphylococcus aureus</span></i><span style="font-family:Verdana;"> were methicillin-resistant (MRSA). Rates of extended spectr</span><span style="font-family:Verdana;">um beta-lactamase-producing enterobacteriaceae (ESBL-PE) ran</span><span style="font-family:Verdana;">ged from 2.5% to 25%. </span><b><span style="font-family:Verdana;">Conclusion:</span></b><span style="font-family:Verdana;"> This study showed an increasing evolution of bacteri</span><span style="font-family:Verdana;">al resistance to antibiotics that </span></span><span style="font-family:Verdana;">are</span><span style="font-family:Verdana;"> spreading throughout Gabon. Th</span><span style="font-family:Verdana;">is constitutes a threat to the health of Gabonese population. 展开更多
关键词 GABON Antibiotic resistance profiles Methicillin-resistant S. aureus (MRSA) Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae (ESBL-PE) CARBAPENEMS
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Urinary Tract Infection among Adults Seeking Medicare at Kiambu Level 5 Hospital, Kenya: Prevalence, Diversity, Antimicrobial Susceptibility Profiles and Possible Risk Factors
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作者 Fredrick Wanja Caroline Ngugi +2 位作者 Eric Omwenga John Maina John Kiiru 《Advances in Microbiology》 2021年第8期360-383,共24页
Urinary tract infections are among the most prevalent extra-intestinal infections, with high prevalence globally. This cross-sectional study established prevalence of bacterial aetiology causing urinary tract infectio... Urinary tract infections are among the most prevalent extra-intestinal infections, with high prevalence globally. This cross-sectional study established prevalence of bacterial aetiology causing urinary tract infection (UTI) and their antimicrobial susceptibility profiles. A questionnaire was used to capture socio-demographic data and possible UTI risk factors among the 206 consented adults seeking medicare at Kiambu Level 5 Hospital. The collected midstream urine samples were subjected to dipstick analysis, microscopy and culture for UTI diagnosis. <b>Results:</b> The overall prevalence rate of UTIs was 27.6%, with women’s prevalence rate being significantly higher at 80.7% compared to men 19.2%. Pregnant women had UTI prevalence at 34% which was higher than other sets of participants. Women who did not frequently change their underpants daily had a higher UTI cases at 34.8%. <i>Escherichia coli</i>, <i>Staphylococcus aureus</i> and <i>Klebsiella pneumoniae</i> were the most prevalent bacterial pathogens at 38.5%, 21% and 19.3%, respectively. Antimicrobial sensitivity analysis revealed high resistances towards Sulfamethoxazole and Ampicillin at range between 50% - 85%, suggesting that these drugs are no longer effective for UTI empirical treatment. The resistance patterns towards Cefotaxime, Cefepime and Ciprofloxacin were below 40%. However, more resistance patterns at a range between 14% - 40% revealed towards Amoxicillin-clavulanic and Nitrofurantoin imply that these are drugs remain potent but there is the need to revise the current UTI management guidelines. In addition, to elude treatment failure, innovation of prophylactic measures is key to halt UTI contraction and offer support to pharmaceutical industries that have fewer new antibiotics in the pipeline. 展开更多
关键词 Urinary Tract Infection (UTI) Risk Factors Antimicrobial resistance and Antimicrobial Susceptibility profile
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