AIM: To investigate the effects of resistin-like molecule β (RELMβ) over-expression on the invasion, metastasis and angiogenesis of gastric cancer cells. METHODS: Human RELMβ encoding expression vec tor was constru...AIM: To investigate the effects of resistin-like molecule β (RELMβ) over-expression on the invasion, metastasis and angiogenesis of gastric cancer cells. METHODS: Human RELMβ encoding expression vec tor was constructed and transfected into the RELMβ lowly-expressed gastric cancer cell lines SGC7901 and MKN-45. Gene expression was measured by Western blotting, reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. Cell proliferation was measured by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry, colony formation and 5-ethynyl-20-deoxyuridine incorporation assays. The in vitro migration, invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenic capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS: Transfection of RELMβ vector into SGC-7901 and MKN-45 cells resulted in over-expression of RELMβ, which did not infl uence the cellular proliferation. However, over-expression of RELMβ suppressed the in vitro adhesion, invasion and metastasis of cancer cells, accompanied by decreased expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, transfection of RELMβ attenuated the expression of vascular endothelial growth factor and in vitro angiogenic capabilities of cancer cells. CONCLUSION: Over-expression of RELMβ abolishes the invasion, metastasis and angiogenesis of gastric cancer cells in vitro, suggesting its potentials as a novel therapeutic target for gastric cancer.展开更多
Helicobacter pylori(H.pylori)was reported to be associated with gastric carcinogenesis.Resistin-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in ga...Helicobacter pylori(H.pylori)was reported to be associated with gastric carcinogenesis.Resistin-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in gastric cancer and correlated with its clinicopathological features.This study aimed to examine the association between H.pylori and RELMp expression in gastric carcinoma and precursor lesions.H.pylori infection and RELMβexpression were immunohistochemically evaluated in gastric biopsies from 230 patients.The biopsies consisted of normal gastric mucosa(w=20),mucosa with chronic gastritis(n=41),intestinal metaplasia(n=42),dysplasia(w=31),intestinal-type adenocarcinoma(n=56),and diffuse-type adenocarcinoma(n=40).RELMβ expression was measured in gastric biopsies after H.pylori eradication therapy in a subgroup of 32 patients.Cultured gastric cancer cell line SGC-7901 was infected with H.pylori strains,and RELMp expression was detected by reverse transcription PCR,real-time PCR and Western blotting.Higher RELMP immunoreactivity was observed in H.pyloripositive intestinal metaplasia(P=0.003),dysplasia(P=0.032),intestinal-type(P=0.037)and diffuse-type adenocarcinomas(P=0.001)than in H.pylori-negative specimens.Expression rates of RELMβ in dysplasia(CO.005),intestinal-type adenocarcinoma(P<0.001),and diffuse-type adenocarcinoma(P=0.001)were significantly correlated with the grade of H.pylori density.In addition,H.pylori eradication reduced the RELMβintensity in intestinal metaplasia(P=0.001).Infection of gastric cancer SGC-7901 cells with cag pathogenicity island(PAI)-positive H.pylori TN2,but not with its PAI totally deleted mutant(TN2-APAI)for 4-8 h,resulted in enhanced protein and transcript levels of RELMβ(P<0.05).In summary,our study suggested that H.pylori infection facilitated the expression of RELMβ in gastric garcinoma and precursor lesions.展开更多
目的:研究抵抗素样分子β(resistin like molecule beta,RELMβ)在慢性阻塞性肺疾病(COPD)患者肺组织中的分布及表达。方法:采集暨南大学附属第一医院因肺癌入院行肺叶切除术的19例肺组织标本,分为COPD组(10例)和对照组(9例)。采用HE染...目的:研究抵抗素样分子β(resistin like molecule beta,RELMβ)在慢性阻塞性肺疾病(COPD)患者肺组织中的分布及表达。方法:采集暨南大学附属第一医院因肺癌入院行肺叶切除术的19例肺组织标本,分为COPD组(10例)和对照组(9例)。采用HE染色、免疫组织化学染色及Western blot方法,检测RELMβ蛋白的表达情况。结果:COPD组患者肺组织中RELMβ的表达(2.657±0.387)明显高于肺功能正常组(0.386±0.072)(P<0.05),且与肺功能主要指标(FEV1%预计值、FEV1/FVC%)呈负相关;与吸烟指数呈正相关(P值均<0.05)。结论:RELMβ可能参与慢性阻塞性肺疾病的发病过程。展开更多
The Aspergillus niger XynB gene and core promoter region of porcine RELMβgene were cloned into pcDNA3.1(-),and an intestine-specific expression vector pcDNA3.1-RELMβ-XynB-Myc-GFP carrying green fluorescence and Myc ...The Aspergillus niger XynB gene and core promoter region of porcine RELMβgene were cloned into pcDNA3.1(-),and an intestine-specific expression vector pcDNA3.1-RELMβ-XynB-Myc-GFP carrying green fluorescence and Myc double tags was constructed.The vector was transfected into human colon cancer cells(HT29)and human liver cancer cells(Bel7402)using liposomes.Fluorescence microscopy revealed that the vector could specifically express green fluorescent protein(GFP)in HT29 cells.RT-PCR and Western Blot were performed on the HT29 cells transfected with the expression vector,and the results showed that the XynB gene was normally transcribed in HT29 cells,and the target protein expression was detected in the cells.展开更多
抵抗素样分子β(resistin-like molecule beta,RELMβ)是近年来发现的参与多种慢性疾病的炎症因子,同时又被称为FIZZ2(found in inflammatory zone 2)。由于RELMβ在疾病过程中起不可忽视的作用,国内外对该蛋白的研究越来越多。目前该...抵抗素样分子β(resistin-like molecule beta,RELMβ)是近年来发现的参与多种慢性疾病的炎症因子,同时又被称为FIZZ2(found in inflammatory zone 2)。由于RELMβ在疾病过程中起不可忽视的作用,国内外对该蛋白的研究越来越多。目前该蛋白在血管生长、平滑肌增殖、气道炎症等方面的作用已有相关研究。本文就RELMβ的结构、调控、生物学功能,重点对RELMβ在肺部疾病和消化道疾病中的作用作一综述。展开更多
基金Supported by The National Natural Science Foundation of China, No. 30200284, No. 30600278, No. 30772359, No. 81071997 and No. 81072073Program for New Century Excellent Talents from Universities, No. NCET-06-0641+1 种基金Scientific Research Fund for the Returned Overseas Chinese Scholars, No. 2008-889Fundamental Research Funds for the Central Universities, No. 2010JC025
文摘AIM: To investigate the effects of resistin-like molecule β (RELMβ) over-expression on the invasion, metastasis and angiogenesis of gastric cancer cells. METHODS: Human RELMβ encoding expression vec tor was constructed and transfected into the RELMβ lowly-expressed gastric cancer cell lines SGC7901 and MKN-45. Gene expression was measured by Western blotting, reverse transcription polymerase chain reaction (PCR) and real-time quantitative PCR. Cell proliferation was measured by 2-(4,5-dimethyltriazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetry, colony formation and 5-ethynyl-20-deoxyuridine incorporation assays. The in vitro migration, invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenic capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS: Transfection of RELMβ vector into SGC-7901 and MKN-45 cells resulted in over-expression of RELMβ, which did not infl uence the cellular proliferation. However, over-expression of RELMβ suppressed the in vitro adhesion, invasion and metastasis of cancer cells, accompanied by decreased expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Moreover, transfection of RELMβ attenuated the expression of vascular endothelial growth factor and in vitro angiogenic capabilities of cancer cells. CONCLUSION: Over-expression of RELMβ abolishes the invasion, metastasis and angiogenesis of gastric cancer cells in vitro, suggesting its potentials as a novel therapeutic target for gastric cancer.
基金the National Natural Science Foundation of China(Nos.81071997,81072073).
文摘Helicobacter pylori(H.pylori)was reported to be associated with gastric carcinogenesis.Resistin-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in gastric cancer and correlated with its clinicopathological features.This study aimed to examine the association between H.pylori and RELMp expression in gastric carcinoma and precursor lesions.H.pylori infection and RELMβexpression were immunohistochemically evaluated in gastric biopsies from 230 patients.The biopsies consisted of normal gastric mucosa(w=20),mucosa with chronic gastritis(n=41),intestinal metaplasia(n=42),dysplasia(w=31),intestinal-type adenocarcinoma(n=56),and diffuse-type adenocarcinoma(n=40).RELMβ expression was measured in gastric biopsies after H.pylori eradication therapy in a subgroup of 32 patients.Cultured gastric cancer cell line SGC-7901 was infected with H.pylori strains,and RELMp expression was detected by reverse transcription PCR,real-time PCR and Western blotting.Higher RELMP immunoreactivity was observed in H.pyloripositive intestinal metaplasia(P=0.003),dysplasia(P=0.032),intestinal-type(P=0.037)and diffuse-type adenocarcinomas(P=0.001)than in H.pylori-negative specimens.Expression rates of RELMβ in dysplasia(CO.005),intestinal-type adenocarcinoma(P<0.001),and diffuse-type adenocarcinoma(P=0.001)were significantly correlated with the grade of H.pylori density.In addition,H.pylori eradication reduced the RELMβintensity in intestinal metaplasia(P=0.001).Infection of gastric cancer SGC-7901 cells with cag pathogenicity island(PAI)-positive H.pylori TN2,but not with its PAI totally deleted mutant(TN2-APAI)for 4-8 h,resulted in enhanced protein and transcript levels of RELMβ(P<0.05).In summary,our study suggested that H.pylori infection facilitated the expression of RELMβ in gastric garcinoma and precursor lesions.
文摘目的:研究抵抗素样分子β(resistin like molecule beta,RELMβ)在慢性阻塞性肺疾病(COPD)患者肺组织中的分布及表达。方法:采集暨南大学附属第一医院因肺癌入院行肺叶切除术的19例肺组织标本,分为COPD组(10例)和对照组(9例)。采用HE染色、免疫组织化学染色及Western blot方法,检测RELMβ蛋白的表达情况。结果:COPD组患者肺组织中RELMβ的表达(2.657±0.387)明显高于肺功能正常组(0.386±0.072)(P<0.05),且与肺功能主要指标(FEV1%预计值、FEV1/FVC%)呈负相关;与吸烟指数呈正相关(P值均<0.05)。结论:RELMβ可能参与慢性阻塞性肺疾病的发病过程。
基金Northern Jiangsu Science and Technology Special Project(XZ-SZ201921)the Technology Program of Gaoyou City(GY201914)。
文摘The Aspergillus niger XynB gene and core promoter region of porcine RELMβgene were cloned into pcDNA3.1(-),and an intestine-specific expression vector pcDNA3.1-RELMβ-XynB-Myc-GFP carrying green fluorescence and Myc double tags was constructed.The vector was transfected into human colon cancer cells(HT29)and human liver cancer cells(Bel7402)using liposomes.Fluorescence microscopy revealed that the vector could specifically express green fluorescent protein(GFP)in HT29 cells.RT-PCR and Western Blot were performed on the HT29 cells transfected with the expression vector,and the results showed that the XynB gene was normally transcribed in HT29 cells,and the target protein expression was detected in the cells.
文摘抵抗素样分子β(resistin-like molecule beta,RELMβ)是近年来发现的参与多种慢性疾病的炎症因子,同时又被称为FIZZ2(found in inflammatory zone 2)。由于RELMβ在疾病过程中起不可忽视的作用,国内外对该蛋白的研究越来越多。目前该蛋白在血管生长、平滑肌增殖、气道炎症等方面的作用已有相关研究。本文就RELMβ的结构、调控、生物学功能,重点对RELMβ在肺部疾病和消化道疾病中的作用作一综述。
