A laser-induced resonance light scattering （RLS） imaging method to determine heparin is described based on the high light scattering emission power of the aggregation species of heparin with α, β, γ, δ-tetra（4-...A laser-induced resonance light scattering （RLS） imaging method to determine heparin is described based on the high light scattering emission power of the aggregation species of heparin with α, β, γ, δ-tetra（4-trimethylaminoniumphenyl）prophyrin （TAPP） in solution, By imaging the light scattering signals of the aggregation species, we proposed the method to determine the heparin with a detection range of 0.02 - 0.6μg/mL and the detection limit （30） of 1.3 ng/mL.展开更多
A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an...A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an aqueous medium with pH value in the range of 3.29—3.78, electrostatic attraction occurs between the negatively charged Evans Blue(EB) and positively charged proteins, forming hydrophobic ion associates and resulting in EB-protein adsorption on H_2O/CCl_4 interface. The presence of cetyltrimethylammonium bromide prompts this adsorption, resulting in strongly enhanced TIR-RLS signals. The intensity of the enhanced TIR-RLS at 360—370 nm was found to be proportional to the concentration of proteins. For bovine serum albumin and human serum albumin, the linear range of detection is 0.07—1.2 μg/mL and the limits of detection are 6.68 and 6.30 ng/mL(3σ), respectively, while for lysozyme, the linear range of detection is 0.06—1.0 μg/mL and the limit of detection is 6.0 ng/mL(3σ). The content of the total albumin in a human urine sample could be directly determined by using the standard addition method with a percent recovery of 97.6%—104.1%, and the RSD ranging from 1.9% to 4.2%.展开更多
The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, hu...The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, human serum albumin, lysozyme, chymotrypsin, resulting in enhanced resonance light scattering(RLS) with the maximal peak at 360 0 nm. With the enhanced RLS, proteins in the range 0-5 0(μg/mL can be determined with the determination limits between 10 3 and 32 2 ng/mL depending on different protein. The effects of foreign substances were tested. The method has been applied to the determination of protein in the synthetic samples and human urine with satisfaction results.展开更多
In the solution containing ammonia,silver nitrate was reduced by tartaric acid,and nano-silver colloids were generated.The products were characterized by transmission electron microscope(TEM) and X-ray diffraction(XRD...In the solution containing ammonia,silver nitrate was reduced by tartaric acid,and nano-silver colloids were generated.The products were characterized by transmission electron microscope(TEM) and X-ray diffraction(XRD).The results show that the average size of silver colloid particles is 25-30 nm and the shape is near-spherical.Researches on the character of resonance light scattering(RLS) of nano-silver colloid indicates that the strongest RLS peak of nano-silver colloid appeares at 447 nm.When BSA adding into the nano-silver colloid solution,the RLS peaks intensity was quenched.Based on this,a novel and simple method of determination of BSA was developed.Under optimum conditions,the linear response is over the range of 2.5pg/mL～2.5×106 pg/mL.展开更多
The aggregation states in methanol-water system and ethanol-water system were studied by resonance light scattering.It was showed that the formation of aggregates results enhanced or reduced resonance light scattering...The aggregation states in methanol-water system and ethanol-water system were studied by resonance light scattering.It was showed that the formation of aggregates results enhanced or reduced resonance light scattering signals characterized by the RLS peaks at 269nm of methanol-water system and at 301nm of ethanol-water system.It was found that their intensity changes were linear within some concentration limits.展开更多
基金supports from the National Natural Science Foundation of China(No.20425517,No.20275032)the Program for New Century Excellent Talents in University(NCET-04-0852)+1 种基金Chun Hui Program(No:[2004]7-24)directed under the Ministry of Education of PRCthe Municipal Science and Technology Committee of Chongqing.
文摘A laser-induced resonance light scattering （RLS） imaging method to determine heparin is described based on the high light scattering emission power of the aggregation species of heparin with α, β, γ, δ-tetra（4-trimethylaminoniumphenyl）prophyrin （TAPP） in solution, By imaging the light scattering signals of the aggregation species, we proposed the method to determine the heparin with a detection range of 0.02 - 0.6μg/mL and the detection limit （30） of 1.3 ng/mL.
文摘A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an aqueous medium with pH value in the range of 3.29—3.78, electrostatic attraction occurs between the negatively charged Evans Blue(EB) and positively charged proteins, forming hydrophobic ion associates and resulting in EB-protein adsorption on H_2O/CCl_4 interface. The presence of cetyltrimethylammonium bromide prompts this adsorption, resulting in strongly enhanced TIR-RLS signals. The intensity of the enhanced TIR-RLS at 360—370 nm was found to be proportional to the concentration of proteins. For bovine serum albumin and human serum albumin, the linear range of detection is 0.07—1.2 μg/mL and the limits of detection are 6.68 and 6.30 ng/mL(3σ), respectively, while for lysozyme, the linear range of detection is 0.06—1.0 μg/mL and the limit of detection is 6.0 ng/mL(3σ). The content of the total albumin in a human urine sample could be directly determined by using the standard addition method with a percent recovery of 97.6%—104.1%, and the RSD ranging from 1.9% to 4.2%.
文摘The interactions of pyrogallol red (PR) with proteins and a derivative method for the determination of proteins were proposed. In the pH range 2 56-4 10, PR interacts with proteins, including bovine serum albumin, human serum albumin, lysozyme, chymotrypsin, resulting in enhanced resonance light scattering(RLS) with the maximal peak at 360 0 nm. With the enhanced RLS, proteins in the range 0-5 0(μg/mL can be determined with the determination limits between 10 3 and 32 2 ng/mL depending on different protein. The effects of foreign substances were tested. The method has been applied to the determination of protein in the synthetic samples and human urine with satisfaction results.
文摘In the solution containing ammonia,silver nitrate was reduced by tartaric acid,and nano-silver colloids were generated.The products were characterized by transmission electron microscope(TEM) and X-ray diffraction(XRD).The results show that the average size of silver colloid particles is 25-30 nm and the shape is near-spherical.Researches on the character of resonance light scattering(RLS) of nano-silver colloid indicates that the strongest RLS peak of nano-silver colloid appeares at 447 nm.When BSA adding into the nano-silver colloid solution,the RLS peaks intensity was quenched.Based on this,a novel and simple method of determination of BSA was developed.Under optimum conditions,the linear response is over the range of 2.5pg/mL～2.5×106 pg/mL.
文摘The aggregation states in methanol-water system and ethanol-water system were studied by resonance light scattering.It was showed that the formation of aggregates results enhanced or reduced resonance light scattering signals characterized by the RLS peaks at 269nm of methanol-water system and at 301nm of ethanol-water system.It was found that their intensity changes were linear within some concentration limits.
