Background: Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children under five years of age in developing countries with viruses contributing significantly to this problem. The rece...Background: Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children under five years of age in developing countries with viruses contributing significantly to this problem. The recently identified parvovirus, Human Bocavirus (HBoV), has also been associated with ARI. Objective: To determine the frequency of HBoV in patients with ARI. Materials and Methods: Samples from 125 consenting patients with influenza like illness signs and symptoms were collected. DNA was extracted from these samples using the QIAamp DNA blood mini kit (Qiagen, Germany). Conventional PCR was carried out and the amplicons were examined in 2% agarose gels stained with ethidium bromide. This was followed by sequencing of the HBoV positive samples. Results: Twenty one (16.8%) patients were found to have HBoV infection. Males (n = 61.9%) were mainly infected with HBoV. Local HBoV strains had 98.9% - 100% similarities and were found to cluster together with other strains obtained elsewhere. Conclusion: These findings suggest that HBoV plays a role in respiratory tract infections in children in Kenya just like it has been found elsewhere. It also sheds light on multiple infections associated with HBoV infections in Kenya.展开更多
Objective To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease Methods PCR assay was used to detect Chlamydia pneumonia specific Pst Ⅰ 474 fragment DNA in swabs from...Objective To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease Methods PCR assay was used to detect Chlamydia pneumonia specific Pst Ⅰ 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis C pneumonia specific antibodies in sera were also assayed with microimmuno fluoresence (MIF) Results About 28% (49/175) of the patients were PCR positive and 25 7%(45/175) were MIF antibodies positive The accordance rate of the two methods was 91 8% Conclusion It is suggested that the C pneumonia infection was common in this group of patients and the C pneumonia Pst Ⅰ474 specific PCR was sensitive and specific for detecting C pneumonia in pharyngolaryngitis or rhinitis and sinusitis展开更多
目的:全面了解病毒和不典型病原体在儿童下呼吸道感染(LRTI)病原中的分布,探讨多重PCR技术检测呼吸道病原的临床应用价值。方法:收集2014年1月至2014年12月温州医科大学附属第二医院育英儿童医院992例因LRTI住院的5岁以下患儿的鼻咽分泌...目的:全面了解病毒和不典型病原体在儿童下呼吸道感染(LRTI)病原中的分布,探讨多重PCR技术检测呼吸道病原的临床应用价值。方法:收集2014年1月至2014年12月温州医科大学附属第二医院育英儿童医院992例因LRTI住院的5岁以下患儿的鼻咽分泌物,用直接免疫荧光法检测呼吸道合胞病毒、流感病毒A、流感病毒B、副流感病毒、腺病毒和衣原体抗原,并提取核酸,采用基于先进片段分析的多重PCR技术测定呼吸道合胞病毒、流感病毒A、流感病毒B、副流感病毒、腺病毒、鼻病毒、博卡病毒、偏肺病毒、冠状病毒和肺炎支原体、衣原体基因。结果:本组患者男662例,女330例,<1岁组587例,1~3岁组287例,3~5岁组118例。多重PCR技术检测结果发现,851例患儿的鼻咽分泌物标本至少存在一种病原阳性(占85.8%),224份标本(占22.6%)检测到两种或两种以上病原。呼吸道合胞病毒和鼻病毒占所有检测病原的前两位。不同年龄组的病原阳性率分别为84.8%、89.2%和82.2%,组间差异无统计学意义(χ~2=4.416,P=0.110)。但<1岁组和1~3岁组的混合感染率均明显高于3~5岁组(χ~2=3.963、9.871,P=0.047、0.002)。鼻病毒可见于各年龄段LRTI患儿,而呼吸道合胞病毒则主要见于婴幼儿。和直接免疫荧光法比较,相同病原检出的一致性较好(Kappa=0.615,P<0.01),但PCR技术病毒的检出率更高(58.37% vs. 41.53%,χ~2=56.23,P<0.001)。结论:呼吸道合胞病毒和鼻病毒占本地区5岁以下儿童LRTI主要病毒和不典型病原的前两位,博卡病毒、冠状病毒和偏肺病毒亦可见,肺炎支原体少见;和直接免疫荧光法比较,多重PCR技术有助于全面了解儿童LRTI的病原。展开更多
文摘Background: Acute respiratory infection (ARI) is a leading cause of morbidity and mortality in children under five years of age in developing countries with viruses contributing significantly to this problem. The recently identified parvovirus, Human Bocavirus (HBoV), has also been associated with ARI. Objective: To determine the frequency of HBoV in patients with ARI. Materials and Methods: Samples from 125 consenting patients with influenza like illness signs and symptoms were collected. DNA was extracted from these samples using the QIAamp DNA blood mini kit (Qiagen, Germany). Conventional PCR was carried out and the amplicons were examined in 2% agarose gels stained with ethidium bromide. This was followed by sequencing of the HBoV positive samples. Results: Twenty one (16.8%) patients were found to have HBoV infection. Males (n = 61.9%) were mainly infected with HBoV. Local HBoV strains had 98.9% - 100% similarities and were found to cluster together with other strains obtained elsewhere. Conclusion: These findings suggest that HBoV plays a role in respiratory tract infections in children in Kenya just like it has been found elsewhere. It also sheds light on multiple infections associated with HBoV infections in Kenya.
文摘Objective To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease Methods PCR assay was used to detect Chlamydia pneumonia specific Pst Ⅰ 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis C pneumonia specific antibodies in sera were also assayed with microimmuno fluoresence (MIF) Results About 28% (49/175) of the patients were PCR positive and 25 7%(45/175) were MIF antibodies positive The accordance rate of the two methods was 91 8% Conclusion It is suggested that the C pneumonia infection was common in this group of patients and the C pneumonia Pst Ⅰ474 specific PCR was sensitive and specific for detecting C pneumonia in pharyngolaryngitis or rhinitis and sinusitis
文摘目的:全面了解病毒和不典型病原体在儿童下呼吸道感染(LRTI)病原中的分布,探讨多重PCR技术检测呼吸道病原的临床应用价值。方法:收集2014年1月至2014年12月温州医科大学附属第二医院育英儿童医院992例因LRTI住院的5岁以下患儿的鼻咽分泌物,用直接免疫荧光法检测呼吸道合胞病毒、流感病毒A、流感病毒B、副流感病毒、腺病毒和衣原体抗原,并提取核酸,采用基于先进片段分析的多重PCR技术测定呼吸道合胞病毒、流感病毒A、流感病毒B、副流感病毒、腺病毒、鼻病毒、博卡病毒、偏肺病毒、冠状病毒和肺炎支原体、衣原体基因。结果:本组患者男662例,女330例,<1岁组587例,1~3岁组287例,3~5岁组118例。多重PCR技术检测结果发现,851例患儿的鼻咽分泌物标本至少存在一种病原阳性(占85.8%),224份标本(占22.6%)检测到两种或两种以上病原。呼吸道合胞病毒和鼻病毒占所有检测病原的前两位。不同年龄组的病原阳性率分别为84.8%、89.2%和82.2%,组间差异无统计学意义(χ~2=4.416,P=0.110)。但<1岁组和1~3岁组的混合感染率均明显高于3~5岁组(χ~2=3.963、9.871,P=0.047、0.002)。鼻病毒可见于各年龄段LRTI患儿,而呼吸道合胞病毒则主要见于婴幼儿。和直接免疫荧光法比较,相同病原检出的一致性较好(Kappa=0.615,P<0.01),但PCR技术病毒的检出率更高(58.37% vs. 41.53%,χ~2=56.23,P<0.001)。结论:呼吸道合胞病毒和鼻病毒占本地区5岁以下儿童LRTI主要病毒和不典型病原的前两位,博卡病毒、冠状病毒和偏肺病毒亦可见,肺炎支原体少见;和直接免疫荧光法比较,多重PCR技术有助于全面了解儿童LRTI的病原。
