Automation advancements prompts the extensive integration of collaborative robot(cobot)across a range of industries.Compared to the commonly used design approach of increasing the payload-to-weight ratio of cobot to e...Automation advancements prompts the extensive integration of collaborative robot(cobot)across a range of industries.Compared to the commonly used design approach of increasing the payload-to-weight ratio of cobot to enhance load capacity,equal attention should be paid to the dynamic response characteristics of cobot during the design process to make the cobot more flexible.In this paper,a new method for designing the drive train parameters of cobot is proposed.Firstly,based on the analysis of factors influencing the load capacity and dynamic response characteristics,design criteria for both aspects are established for cobot with all optimization design criteria normalized within the design domain.Secondly,with the cobot in the horizontal pose,the motor design scheme is discretized and it takes the joint motor diameter and gearbox speed ratio as optimization design variables.Finally,all the discrete values of the optimization objectives are obtained through the enumeration method and the Pareto front is used to select the optimal solution through multi-objective optimization.Base on the cobot design method proposed in this paper,a six-axis cobot is designed and compared with the commercial cobot.The result shows that the load capacity of the designed cobot in this paper reaches 8.4 kg,surpassing the 5 kg load capacity commercial cobot which is used as a benchmark.The minimum resonance frequency of the joints is 42.70 Hz.展开更多
This paper presents an expert-based fuzzy analytic hierarchy process( AHP) model for evaluating emergency response capacity of Chemical Industrial Park( ERCCIP) by jointly using an improved fuzzy preference programmin...This paper presents an expert-based fuzzy analytic hierarchy process( AHP) model for evaluating emergency response capacity of Chemical Industrial Park( ERCCIP) by jointly using an improved fuzzy preference programming( FPP) and 2-tuple fuzzy linguistic approach. An evaluation index system for ERCCIP is proposed. The weight of sub-criteria and criteria of the evaluation index system for ERCCIP are determined using the improved FPP. And the ratings of sub-criteria are assessed in linguistic values according to the experts' subjective opinions. Finally,the aggregated ratings of criteria and the overall ERCCIP are calculated.展开更多
Objective: To study the effect of vitamin C combined with vitamin E on antioxidant capacity and inflammatory response in patients with hemodialysis. Methods: A total of 116 patients with uremia who received hemodialys...Objective: To study the effect of vitamin C combined with vitamin E on antioxidant capacity and inflammatory response in patients with hemodialysis. Methods: A total of 116 patients with uremia who received hemodialysis in the hospital between December 2014 and January 2017 were collected and divided into control group and observation group by random number table, each with 58 cases. Control group received vitamin C intervention, and observation group received vitamin C combined with vitamin E intervention. The differences in serum levels of antioxidant indexes, pro-inflammatory mediators and anti-inflammatory mediators were compared between the two groups before intervention and 6 weeks after intervention. Results: Before intervention, serum levels of antioxidant indexes, pro-inflammatory mediators and anti-inflammatory mediators were not significantly different between the two groups. After intervention, serum SOD, GSH-PX and T-AOC levels of both groups of patients were higher than those before treatment while IL-1, IL-2, IL-6, IL-8, IL-4, IL-10 and IL-13 levels were lower than those before treatment, and serum SOD, GSH-PX and T-AOC levels of observation group were higher than those of control group while IL-1, IL-2, IL-6, IL-8, IL-4, IL-10 and IL-13 levels were lower than those of control group. Conclusion: Vitamin C combined with vitamin E intervention can effectively enhance the antioxidant capacity and suppress the systemic inflammatory state in patients with hemodialysis.展开更多
The majority of rice(Oryza sativa L.) produced in the southern USA is drill-seeded and grown under upland-like conditions because permanent flooding is established after the four-leaf stage. Therefore, rice during the...The majority of rice(Oryza sativa L.) produced in the southern USA is drill-seeded and grown under upland-like conditions because permanent flooding is established after the four-leaf stage. Therefore, rice during the seedling growth stage will be subjected to variable soil moisture content. A greenhouse experiment was conducted to evaluate the performance of 15 rice cultivars commonly grown in Mississippi of USA under early-season soil moisture stress. Twenty morpho-physiological parameters of rice seedlings subjected to three different levels(100%, 66% and 33% field capacity) of soil moisture, from 10 to 30 d after sowing, were measured. Significant moisture stress × treatment interaction(P < 0.001) was observed for most of the parameters. Further, the total drought response index(TDRI) was developed to score the cultivars for drought tolerance with the variation from 26.88 to 36.21. Accordingly, the cultivars were classified into different groups of tolerance. The cultivars CL152 and CL142-AR were classified as the least and the most tolerant to drought based on TDRI and standard deviation, respectively. Even though both total root(R^2 = 0.98) or shoot(R^2 = 0.76) drought responses indices were positively correlated with TDRI, root traits were important in deriving the indices. Therefore, TDRI could be used to select cultivars for drought tolerance in a given environment and develop rice varieties with early-season drought tolerance. However, further research is needed to identify and characterize tolerance at other stages to assist breeding programs in rice.展开更多
This paper presents a reliability analysis of the pseudo-static seismic bearing capacity of a strip foundation using the limit equilibrium theory. The first-order reliability method(FORM) is employed to calculate the ...This paper presents a reliability analysis of the pseudo-static seismic bearing capacity of a strip foundation using the limit equilibrium theory. The first-order reliability method(FORM) is employed to calculate the reliability index. The response surface methodology(RSM) is used to assess the Hasofer e Lind reliability index and then it is optimized using a genetic algorithm(GA). The random variables used are the soil shear strength parameters and the seismic coefficients(khand kv). Two assumptions(normal and non-normal distribution) are used for the random variables. The assumption of uncorrelated variables was found to be conservative in comparison to that of negatively correlated soil shear strength parameters. The assumption of non-normal distribution for the random variables can induce a negative effect on the reliability index of the practical range of the seismic bearing capacity.展开更多
The integrated energy system(IES)is an important energy supply method for mitigating the energy crisis.A station-and-network–coordinated planning method for the IES,which considers the integrated demand responses(IDR...The integrated energy system(IES)is an important energy supply method for mitigating the energy crisis.A station-and-network–coordinated planning method for the IES,which considers the integrated demand responses(IDRs)of flexible loads,electric vehicles,and energy storage is proposed in this work.First,based on load substitution at the user side,an energy-station model considering the IDR is established.Then,based on the characteristics of the energy network,a collaborative planning model is established for the energy station and energy network of the IES,considering the comprehensive system investment,operation and maintenance,and clean energy shortage penalty costs,to minimize the total cost.This can help optimize the locations of the power lines and natural gas pipelines and the capacities of the equipment in an energy station.Finally,simulations are performed to demonstrate that the proposed planning method can help delay or reduce the construction of new lines and energy-station equipment,thereby reducing the investment required and improving the planning economics of the IES.展开更多
Different techniques have been proposed to increase the bearing capacity of open-ended piles.Welding helices to the shaft and tapering the pile shaft could be used simultaneously to enhance the static and dynamic beha...Different techniques have been proposed to increase the bearing capacity of open-ended piles.Welding helices to the shaft and tapering the pile shaft could be used simultaneously to enhance the static and dynamic behaviors of these piles.This paper subjects the bearing capacity,stiffness,frictional behavior,and material efficiency of the tapered helical piles to scrutiny.Tapered helical piles are introduced herein as an alternative option to improve the material efficiency of hollow piles.Based on the Taguchi method,a series of experiments was designed and conducted.The axial responses of tapered helical piles are also investigated using finite element analyses.The results derived from loadedisplacement curves and strain gages are used to characterize the axial compression responses of tapered helical piles.The effects of tapered angle,helices diameter and helices distance are examined using dimensionless parameters,and the degree of contribution of these factors is calculated on each of the enumerated variables individually.Experimental results show that the shaft friction resistance of tapered helical piles increases continuously with the pile head settlement.Furthermore,the effect of tapered wall on the shaft friction resistance is more tangible at low stress levels.The results showed that the relative material efficiency factor of the optimum pile could be 2.5 times that of unoptimized pile with a similar quantity of material.展开更多
The current design philosophy for submarine hulls,in the preliminary design stage,generally considers as governing limit states material yielding along with various buckling modes.It is common belief that,beyond the d...The current design philosophy for submarine hulls,in the preliminary design stage,generally considers as governing limit states material yielding along with various buckling modes.It is common belief that,beyond the design pressure,material yielding of the shell plating should occur first,eventually followed by local buckling,while global buckling currently retains the highest safety factor.On the other hand,in the aeronautical field,in some cases structural components are designed in such a way that local instability may occur within the design loads,being the phenomena inside the material elastic range and not leading to a significant drop in term of stiffness.This paper is aimed at investigating the structural response beyond a set of selected limit states,using nonlinear FE method adopting different initial imperfection models,to provide the designers with new information useful for calibrating safety factors.It was found that both local and global buckling can be considered as ultimate limit states,with a significant sensitivity towards initial imperfection,while material yielding and tripping buckling of frames show a residual structural capacity.In conclusion,it was found that the occurrence of local buckling leads to similar sudden catastrophic consequences as global buckling,with the ultimate strength capacity highly affected by the initial imperfection shape and amplitude.展开更多
This paper presents a new procedure to transform an SSI system into an equivalent SDOF system using twice equivalence. A pushover analysis procedure based on the capacity spectrum method for buildings with SSI effects...This paper presents a new procedure to transform an SSI system into an equivalent SDOF system using twice equivalence. A pushover analysis procedure based on the capacity spectrum method for buildings with SSI effects (PASSI) is then established based on the equivalent SDOF system, and the modified response spectrum and equivalent capacity spectrum are obtained. Furthermore, the approximate formulas to obtain the dynamic stiffness of foundations are suggested. Three steel buildings with different story heights (3, 9 and 20) including SSI effects are analyzed under two far-field and two near-field historical records and an artificial seismic time history using the two PASSI procedures and the nonlinear response history analysis (NLhRHA) method. The results are compared and discussed. Finally, combined with seismic design response spectrum, the nonlinear seismic response of a 9-story building with SSI effects is analyzed using the PASSI procedures, and its seismic performance is evaluated according to the Chinese 'Code for Seismic Design of Buildings. The feasibility of the proposed procedure is verified.展开更多
Immune function and antioxidant defense play an important role in protecting animals against pathogens and in controlling oxidative stress, respectively. Aldicarb is of great concern for human health due to its toxic ...Immune function and antioxidant defense play an important role in protecting animals against pathogens and in controlling oxidative stress, respectively. Aldicarb is of great concern for human health due to its toxic nature, its extensive usage and consequent pollution. The hypothesis that aldicarb exposure would suppress immune function and antioxidant capacity in Kunming mice was to be tested in the present study. Twenty-three adult male mice were randomly divided into the control (n = 11) and the aldicarb treated (n = 12) groups. Food and water were provided ad libtum for both groups, while the aldicarb treated mice drank aldicarb solution (0.097 mg/L) for 22 days. Cellular immunity assessed by phytohaemagglutinin (PHA) response did not differ between the control and the aldicarb treated groups. Similarly, white blood cells were not influenced by aldicarb treatment. Moreover, aldicarb exposure had not significant effect on body mass, all organ masses detected. However, aldicarb treatment suppressed total antioxidant capacity in liver but not in kidneys. In summary, aldicarb treatment did not affect immune function, but suppressed liver antioxidant capacity in Kunming mice.展开更多
Land subsidence has severe physical and economic implications for both areas and people. Numerous scholars have shown that land subsidence has had massive impacts at global, national and regional levels, and that the ...Land subsidence has severe physical and economic implications for both areas and people. Numerous scholars have shown that land subsidence has had massive impacts at global, national and regional levels, and that the impacts were usually responded to by the government. However, little attention has been paid to what land subsidence means to people’s daily lives and how much it costs them. To fill that gap, this article draws on empirical research carried out in three areas in Indonesia to provide a better understanding of what land subsidence means to households, and how they respond to the consequences and how much it costs them to do so. An analysis of a survey of 330 households shows that they have been suffering from various severities of impacts of land subsidence for an extended period. Whereas some of the households respond to the impacts by making small preparations or adapting to the damages, others can do nothing due to a lack of money and their continuously declining earning capacity. Thus, the affected households are effectively throwing money into a bottomless pit. We argue that these households must escape the vicious circle caused by land subsidence by increasing their income capacity or even abandoning the affected areas.展开更多
In this study, the immuno-response of recombinant hCG-β and natural hCGβ was comparatively investigated by using Freund's adjuvant. The results showed that, the properties and merits of the antibodies elicited b...In this study, the immuno-response of recombinant hCG-β and natural hCGβ was comparatively investigated by using Freund's adjuvant. The results showed that, the properties and merits of the antibodies elicited by both kinds of hCG-β were similar. The antisera had high affinity for binding with hCG (Kαγβ≈5.86×108/mol/L, Kαβ≈8.18×108/mol/L), and were found to be effective in inhibiting the binding of 125I-hCG to receptors in rat testes. Results also indicated that, similar to the antisera induced by natural hCG-β, the recombinant hCG-β induced antisera had capacity of neutralizing the biological activities of hCG. Recombinant hCG-β could be used as an immunogen for contraceptive vaccine.展开更多
基金Supported by National Key Research and Development Program of China (Grant Nos.2022YFB4703000,2019YFB1309900)。
文摘Automation advancements prompts the extensive integration of collaborative robot(cobot)across a range of industries.Compared to the commonly used design approach of increasing the payload-to-weight ratio of cobot to enhance load capacity,equal attention should be paid to the dynamic response characteristics of cobot during the design process to make the cobot more flexible.In this paper,a new method for designing the drive train parameters of cobot is proposed.Firstly,based on the analysis of factors influencing the load capacity and dynamic response characteristics,design criteria for both aspects are established for cobot with all optimization design criteria normalized within the design domain.Secondly,with the cobot in the horizontal pose,the motor design scheme is discretized and it takes the joint motor diameter and gearbox speed ratio as optimization design variables.Finally,all the discrete values of the optimization objectives are obtained through the enumeration method and the Pareto front is used to select the optimal solution through multi-objective optimization.Base on the cobot design method proposed in this paper,a six-axis cobot is designed and compared with the commercial cobot.The result shows that the load capacity of the designed cobot in this paper reaches 8.4 kg,surpassing the 5 kg load capacity commercial cobot which is used as a benchmark.The minimum resonance frequency of the joints is 42.70 Hz.
基金Sponsored by the National Natural Science Foundation of China(Grant No.41001354)Fundamental Research Funds for the Central Universities of China(Grant No.23420110083)
文摘This paper presents an expert-based fuzzy analytic hierarchy process( AHP) model for evaluating emergency response capacity of Chemical Industrial Park( ERCCIP) by jointly using an improved fuzzy preference programming( FPP) and 2-tuple fuzzy linguistic approach. An evaluation index system for ERCCIP is proposed. The weight of sub-criteria and criteria of the evaluation index system for ERCCIP are determined using the improved FPP. And the ratings of sub-criteria are assessed in linguistic values according to the experts' subjective opinions. Finally,the aggregated ratings of criteria and the overall ERCCIP are calculated.
文摘Objective: To study the effect of vitamin C combined with vitamin E on antioxidant capacity and inflammatory response in patients with hemodialysis. Methods: A total of 116 patients with uremia who received hemodialysis in the hospital between December 2014 and January 2017 were collected and divided into control group and observation group by random number table, each with 58 cases. Control group received vitamin C intervention, and observation group received vitamin C combined with vitamin E intervention. The differences in serum levels of antioxidant indexes, pro-inflammatory mediators and anti-inflammatory mediators were compared between the two groups before intervention and 6 weeks after intervention. Results: Before intervention, serum levels of antioxidant indexes, pro-inflammatory mediators and anti-inflammatory mediators were not significantly different between the two groups. After intervention, serum SOD, GSH-PX and T-AOC levels of both groups of patients were higher than those before treatment while IL-1, IL-2, IL-6, IL-8, IL-4, IL-10 and IL-13 levels were lower than those before treatment, and serum SOD, GSH-PX and T-AOC levels of observation group were higher than those of control group while IL-1, IL-2, IL-6, IL-8, IL-4, IL-10 and IL-13 levels were lower than those of control group. Conclusion: Vitamin C combined with vitamin E intervention can effectively enhance the antioxidant capacity and suppress the systemic inflammatory state in patients with hemodialysis.
基金funded by Mississippi Rice Promotion Board and United States Department of AgricultureNational Institute of Food and Agriculture(USDA-NIFA,Grant No.2013-34263-20931)sub-award to Mississippi State University(Grant No.G-7799-2)
文摘The majority of rice(Oryza sativa L.) produced in the southern USA is drill-seeded and grown under upland-like conditions because permanent flooding is established after the four-leaf stage. Therefore, rice during the seedling growth stage will be subjected to variable soil moisture content. A greenhouse experiment was conducted to evaluate the performance of 15 rice cultivars commonly grown in Mississippi of USA under early-season soil moisture stress. Twenty morpho-physiological parameters of rice seedlings subjected to three different levels(100%, 66% and 33% field capacity) of soil moisture, from 10 to 30 d after sowing, were measured. Significant moisture stress × treatment interaction(P < 0.001) was observed for most of the parameters. Further, the total drought response index(TDRI) was developed to score the cultivars for drought tolerance with the variation from 26.88 to 36.21. Accordingly, the cultivars were classified into different groups of tolerance. The cultivars CL152 and CL142-AR were classified as the least and the most tolerant to drought based on TDRI and standard deviation, respectively. Even though both total root(R^2 = 0.98) or shoot(R^2 = 0.76) drought responses indices were positively correlated with TDRI, root traits were important in deriving the indices. Therefore, TDRI could be used to select cultivars for drought tolerance in a given environment and develop rice varieties with early-season drought tolerance. However, further research is needed to identify and characterize tolerance at other stages to assist breeding programs in rice.
基金the Ministry of Higher Education and Scientific Research of Algeria for supporting this work by offering an 11-month scholarship to the first author at the 3SR laboratory of Grenoble Alpes University,France
文摘This paper presents a reliability analysis of the pseudo-static seismic bearing capacity of a strip foundation using the limit equilibrium theory. The first-order reliability method(FORM) is employed to calculate the reliability index. The response surface methodology(RSM) is used to assess the Hasofer e Lind reliability index and then it is optimized using a genetic algorithm(GA). The random variables used are the soil shear strength parameters and the seismic coefficients(khand kv). Two assumptions(normal and non-normal distribution) are used for the random variables. The assumption of uncorrelated variables was found to be conservative in comparison to that of negatively correlated soil shear strength parameters. The assumption of non-normal distribution for the random variables can induce a negative effect on the reliability index of the practical range of the seismic bearing capacity.
基金supported in part by the National Key R&D Program of China(2018YFB0905000)the Science and Technology Project of the State Grid Corporation of China(SGTJDK00DWJS1800232)
文摘The integrated energy system(IES)is an important energy supply method for mitigating the energy crisis.A station-and-network–coordinated planning method for the IES,which considers the integrated demand responses(IDRs)of flexible loads,electric vehicles,and energy storage is proposed in this work.First,based on load substitution at the user side,an energy-station model considering the IDR is established.Then,based on the characteristics of the energy network,a collaborative planning model is established for the energy station and energy network of the IES,considering the comprehensive system investment,operation and maintenance,and clean energy shortage penalty costs,to minimize the total cost.This can help optimize the locations of the power lines and natural gas pipelines and the capacities of the equipment in an energy station.Finally,simulations are performed to demonstrate that the proposed planning method can help delay or reduce the construction of new lines and energy-station equipment,thereby reducing the investment required and improving the planning economics of the IES.
文摘Different techniques have been proposed to increase the bearing capacity of open-ended piles.Welding helices to the shaft and tapering the pile shaft could be used simultaneously to enhance the static and dynamic behaviors of these piles.This paper subjects the bearing capacity,stiffness,frictional behavior,and material efficiency of the tapered helical piles to scrutiny.Tapered helical piles are introduced herein as an alternative option to improve the material efficiency of hollow piles.Based on the Taguchi method,a series of experiments was designed and conducted.The axial responses of tapered helical piles are also investigated using finite element analyses.The results derived from loadedisplacement curves and strain gages are used to characterize the axial compression responses of tapered helical piles.The effects of tapered angle,helices diameter and helices distance are examined using dimensionless parameters,and the degree of contribution of these factors is calculated on each of the enumerated variables individually.Experimental results show that the shaft friction resistance of tapered helical piles increases continuously with the pile head settlement.Furthermore,the effect of tapered wall on the shaft friction resistance is more tangible at low stress levels.The results showed that the relative material efficiency factor of the optimum pile could be 2.5 times that of unoptimized pile with a similar quantity of material.
基金The research activity on this topic is still under development in the frame of the ASAMS(Aspetti specialistici e approccio metodologico per progettazione di sottomarini di ultima generazione)project(2019-2022)which has been funded by the Italian MoD–Segredifesa,in collaboration with Fincantieri.
文摘The current design philosophy for submarine hulls,in the preliminary design stage,generally considers as governing limit states material yielding along with various buckling modes.It is common belief that,beyond the design pressure,material yielding of the shell plating should occur first,eventually followed by local buckling,while global buckling currently retains the highest safety factor.On the other hand,in the aeronautical field,in some cases structural components are designed in such a way that local instability may occur within the design loads,being the phenomena inside the material elastic range and not leading to a significant drop in term of stiffness.This paper is aimed at investigating the structural response beyond a set of selected limit states,using nonlinear FE method adopting different initial imperfection models,to provide the designers with new information useful for calibrating safety factors.It was found that both local and global buckling can be considered as ultimate limit states,with a significant sensitivity towards initial imperfection,while material yielding and tripping buckling of frames show a residual structural capacity.In conclusion,it was found that the occurrence of local buckling leads to similar sudden catastrophic consequences as global buckling,with the ultimate strength capacity highly affected by the initial imperfection shape and amplitude.
基金National Natural Science Foundation of China Under Granted No.50538020Youth Science Foundation of Harbin City Under Grand No.2005AFXXJ015Youth Science Foundation of Heilongjiang Institute of Science and Technology
文摘This paper presents a new procedure to transform an SSI system into an equivalent SDOF system using twice equivalence. A pushover analysis procedure based on the capacity spectrum method for buildings with SSI effects (PASSI) is then established based on the equivalent SDOF system, and the modified response spectrum and equivalent capacity spectrum are obtained. Furthermore, the approximate formulas to obtain the dynamic stiffness of foundations are suggested. Three steel buildings with different story heights (3, 9 and 20) including SSI effects are analyzed under two far-field and two near-field historical records and an artificial seismic time history using the two PASSI procedures and the nonlinear response history analysis (NLhRHA) method. The results are compared and discussed. Finally, combined with seismic design response spectrum, the nonlinear seismic response of a 9-story building with SSI effects is analyzed using the PASSI procedures, and its seismic performance is evaluated according to the Chinese 'Code for Seismic Design of Buildings. The feasibility of the proposed procedure is verified.
文摘Immune function and antioxidant defense play an important role in protecting animals against pathogens and in controlling oxidative stress, respectively. Aldicarb is of great concern for human health due to its toxic nature, its extensive usage and consequent pollution. The hypothesis that aldicarb exposure would suppress immune function and antioxidant capacity in Kunming mice was to be tested in the present study. Twenty-three adult male mice were randomly divided into the control (n = 11) and the aldicarb treated (n = 12) groups. Food and water were provided ad libtum for both groups, while the aldicarb treated mice drank aldicarb solution (0.097 mg/L) for 22 days. Cellular immunity assessed by phytohaemagglutinin (PHA) response did not differ between the control and the aldicarb treated groups. Similarly, white blood cells were not influenced by aldicarb treatment. Moreover, aldicarb exposure had not significant effect on body mass, all organ masses detected. However, aldicarb treatment suppressed total antioxidant capacity in liver but not in kidneys. In summary, aldicarb treatment did not affect immune function, but suppressed liver antioxidant capacity in Kunming mice.
文摘Land subsidence has severe physical and economic implications for both areas and people. Numerous scholars have shown that land subsidence has had massive impacts at global, national and regional levels, and that the impacts were usually responded to by the government. However, little attention has been paid to what land subsidence means to people’s daily lives and how much it costs them. To fill that gap, this article draws on empirical research carried out in three areas in Indonesia to provide a better understanding of what land subsidence means to households, and how they respond to the consequences and how much it costs them to do so. An analysis of a survey of 330 households shows that they have been suffering from various severities of impacts of land subsidence for an extended period. Whereas some of the households respond to the impacts by making small preparations or adapting to the damages, others can do nothing due to a lack of money and their continuously declining earning capacity. Thus, the affected households are effectively throwing money into a bottomless pit. We argue that these households must escape the vicious circle caused by land subsidence by increasing their income capacity or even abandoning the affected areas.
文摘In this study, the immuno-response of recombinant hCG-β and natural hCGβ was comparatively investigated by using Freund's adjuvant. The results showed that, the properties and merits of the antibodies elicited by both kinds of hCG-β were similar. The antisera had high affinity for binding with hCG (Kαγβ≈5.86×108/mol/L, Kαβ≈8.18×108/mol/L), and were found to be effective in inhibiting the binding of 125I-hCG to receptors in rat testes. Results also indicated that, similar to the antisera induced by natural hCG-β, the recombinant hCG-β induced antisera had capacity of neutralizing the biological activities of hCG. Recombinant hCG-β could be used as an immunogen for contraceptive vaccine.