ISSR molecular marker technology was adopted to conduct comparison analysis on genetic diversity level and population genetic structure of Beauveria bassiana population in natural secondary forest （Langyashan nationa...ISSR molecular marker technology was adopted to conduct comparison analysis on genetic diversity level and population genetic structure of Beauveria bassiana population in natural secondary forest （Langyashan national forest park） in Chuzhou City of Anhui Province and artificial pure pine forest （Magushan forest farm） in Xuancheng City of Anhui Province.Seven primers were selected to conduct PCR amplification on total 222 strains of B.bassiana in two populations,a total of 58 unique amplified loci were obtained through amplification,the number of polymorphic loci was 56,the percentage of polymorphic loci was 96.55%,Nei＇s genetic diversity was 0.299 3,Shannon information index was 0.459 3,genetic differentiation coefficient among populations （Gst） was 0.128 3,gene flow Nm=3.398 4;the gene flow between the two populations was small,genetic differentiation was relatively large,being 12.83%,this may be caused by human selective pressures and barrier of gene flow;the genetic variation level of B.bassiana populations in Langyashan was relatively high（PPL=96.55%,H=0.278 1,I= 0.429 9）;the genetic variation level of B.bassiana populations in Magushan was relatively low（PPL=93.10%,H=0.255 2,I= 0.382 5）.The genetic diversity of B.bassiana from the primary forest in Dabieshan was studied（PPL=81.00%,H=0.318 7,I= 0.478 2）,indicating that the genetic diversity of B.bassiana populations in Dabieshan with complex ecological environment was the highest,followed by the populations in natural secondary forest,and the genetic diversity in artificial pure pine forest was the lowest.Nei＇s genetic distance was adopted to construct the genetic relationship dendrogram of B.bassiana individuals collected from Langyashan and Magushan,from the cluster analysis of UPGMA,the strains from the same collection places clustered together.展开更多
Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many use...Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many uses, easiness of tissue culture and small gene group that make them well suited as a model system for the application of genetic engineering in forest trees. In the last decade, much progress has been made in genetic improvement of poplar species in China. Modern biotechnology is an important tool for genetic improvement in forest trees, and its applications to genetic improvement in poplars, which covers genetic transformation, gene expression, construction of genetic linkage map, QTLs (quantitative trait loci) identification and molecular assisted selection are reviewed in this paper. At the same time, the existing problems and outlook about the application of modern biotechnology to genetic improvement in forest trees are also discussed.展开更多
A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populati...A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populations were constructed by crossing with indica cultivars TN1 and Zhefu 802,respectively.Genetic analysis demonstrated that the phenotype was controlled by a single recessive nuclear gene.The closely linked simple sequence repeat(SSR) marker RM1155 was obtained from bulked segregant analysis.Subsequently,sequence tagged site(STS) markers were developed using the published rice genome sequence.Finally,RTL1 was located between an STS marker T1591 and an SSR marker RM1359,at the distances of 0.48 cM and 0.96 cM,respectively.These results will facilitate the cloning of the target gene in further studies.展开更多
The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resourc...The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resource of poplars in China is replete. Over the past 10 years, the application of molecular biological techniques to genetic improvements in poplar species has been widely studied in China. Recent advances in molecular genetic improvements of poplar, including cDNA library construction, gene cloning and identification, genetic engineering, gene expression, genetic linkage map construction, mapping of quantitative trait loci （QTL） and molecular-assisted selection, are reviewed in the present paper. In addition, the application of modern biotechnology to molecular improvements in the genetic traits of the poplar and some unsolved problems are discussed.展开更多
An F<sub>2</sub> population developed from the Xa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene, Xa-4. Some restriction fragment length polym...An F<sub>2</sub> population developed from the Xa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene, Xa-4. Some restriction fragment length polymorphism （RFLP） markers on the high-density map constructed by Harushima et al. and the amplified DMA fragments homologous to the conserved domains of plant disease resistance （R） genes were used to construct the genetic linkage map around the gene Xa-4 by scoring susceptible individuals in the population. Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction （PCR） marker M55. The R gene homologous fragment marker RS13 was found co-segregating with Xa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker RS13 can be applied to molecular marker-assisted selection of Xa-4 in rice breeding programs.展开更多
Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1,had been identified in an indica ...Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1,had been identified in an indica rice variety, Aitaiyin 2. In this study the gene was genetically mapped by using an F2 population, which consisted of 474 individuals developed from a cross between Aitaiyin 2 and B30. The sd-t(t) gene was located between the RFLP markers R514 and R1408B with a distance of 1.1 cM to R514, and 4.5 cM to R1408B on chromosome 4. A physical contig covering the sd-t(t) mapping region was further constructed by screening a BAC library with R514 and R1408B as probes, and the physical distance between R514 and R1408B was estimated at approximately 147 kb. This result will facilitate map-based cloning of the sd-t(t) gene.展开更多
西瓜炭疽病(Anthracnose)是由瓜类炭疽病菌(Colletotrichumorbiculare)引起的真菌性病害。本研究以抗病自交系PI189225和感病自交系Black Diamond杂交并自交获得F1、F2、F3为材料,采用炭疽病菌生理小种1接种,对西瓜抗炭疽病生理小种1进...西瓜炭疽病(Anthracnose)是由瓜类炭疽病菌(Colletotrichumorbiculare)引起的真菌性病害。本研究以抗病自交系PI189225和感病自交系Black Diamond杂交并自交获得F1、F2、F3为材料,采用炭疽病菌生理小种1接种,对西瓜抗炭疽病生理小种1进行遗传规律分析和基因定位研究。研究结果表明,西瓜炭疽病抗性基因由显性单基因控制,抗病对感病为显性,将此基因命名为Rco-1。用分离群体分组分析法(BSA)和AFLP分子标记技术对PI189225中的抗炭疽病基因进行分子标记鉴定,并利用MAPMAKER/Exp version 3.0软件进行了标记与目的基因间的遗传距离计算,发现E4/M19、E1/M8、E29/M5与抗炭疽病基因Rco-1连锁,遗传距离分别为34.8、23.4、6.9cM。为采用分子标记辅助选育抗炭疽病西瓜新品种奠定了基础。展开更多
基金Supported by Youth Science Fund in Anhui Agricultural UniversityNational Natural Science Foundation (30972368)Excellent Youth Science Foundation in Anhui Province (08040106902)~~
文摘ISSR molecular marker technology was adopted to conduct comparison analysis on genetic diversity level and population genetic structure of Beauveria bassiana population in natural secondary forest （Langyashan national forest park） in Chuzhou City of Anhui Province and artificial pure pine forest （Magushan forest farm） in Xuancheng City of Anhui Province.Seven primers were selected to conduct PCR amplification on total 222 strains of B.bassiana in two populations,a total of 58 unique amplified loci were obtained through amplification,the number of polymorphic loci was 56,the percentage of polymorphic loci was 96.55%,Nei＇s genetic diversity was 0.299 3,Shannon information index was 0.459 3,genetic differentiation coefficient among populations （Gst） was 0.128 3,gene flow Nm=3.398 4;the gene flow between the two populations was small,genetic differentiation was relatively large,being 12.83%,this may be caused by human selective pressures and barrier of gene flow;the genetic variation level of B.bassiana populations in Langyashan was relatively high（PPL=96.55%,H=0.278 1,I= 0.429 9）;the genetic variation level of B.bassiana populations in Magushan was relatively low（PPL=93.10%,H=0.255 2,I= 0.382 5）.The genetic diversity of B.bassiana from the primary forest in Dabieshan was studied（PPL=81.00%,H=0.318 7,I= 0.478 2）,indicating that the genetic diversity of B.bassiana populations in Dabieshan with complex ecological environment was the highest,followed by the populations in natural secondary forest,and the genetic diversity in artificial pure pine forest was the lowest.Nei＇s genetic distance was adopted to construct the genetic relationship dendrogram of B.bassiana individuals collected from Langyashan and Magushan,from the cluster analysis of UPGMA,the strains from the same collection places clustered together.
文摘Poplars are among the most important deciduous tree species in China. China is replete with natural resources of poplars. Poplars have a number of good characteristics, including fast growth rate, high yield, many uses, easiness of tissue culture and small gene group that make them well suited as a model system for the application of genetic engineering in forest trees. In the last decade, much progress has been made in genetic improvement of poplar species in China. Modern biotechnology is an important tool for genetic improvement in forest trees, and its applications to genetic improvement in poplars, which covers genetic transformation, gene expression, construction of genetic linkage map, QTLs (quantitative trait loci) identification and molecular assisted selection are reviewed in this paper. At the same time, the existing problems and outlook about the application of modern biotechnology to genetic improvement in forest trees are also discussed.
基金supported by the National Major Special Program of Breeding of Transgenetic Organisms New Variety(Grant Nos.2009ZX08001-022B,2009ZX08009-125B)National Natural ScienceFoundation of China(Grant No.30970171)
文摘A rumpled and twisted leaf 1(rtl1) mutant was generated from a japonica cultivar Nipponbare by ethyl methanesulfonate treatment,which was characterized as rumpled and twisted leaf at the seedling stage.The F2 populations were constructed by crossing with indica cultivars TN1 and Zhefu 802,respectively.Genetic analysis demonstrated that the phenotype was controlled by a single recessive nuclear gene.The closely linked simple sequence repeat(SSR) marker RM1155 was obtained from bulked segregant analysis.Subsequently,sequence tagged site(STS) markers were developed using the published rice genome sequence.Finally,RTL1 was located between an STS marker T1591 and an SSR marker RM1359,at the distances of 0.48 cM and 0.96 cM,respectively.These results will facilitate the cloning of the target gene in further studies.
基金Supported by the National Natural Science Foundation of China （30271093）
文摘The poplar is one of the most economically important and intensively studied tree species owing to its wide application in the timber industry and as a model material for the study of woody plants. The natural resource of poplars in China is replete. Over the past 10 years, the application of molecular biological techniques to genetic improvements in poplar species has been widely studied in China. Recent advances in molecular genetic improvements of poplar, including cDNA library construction, gene cloning and identification, genetic engineering, gene expression, genetic linkage map construction, mapping of quantitative trait loci （QTL） and molecular-assisted selection, are reviewed in the present paper. In addition, the application of modern biotechnology to molecular improvements in the genetic traits of the poplar and some unsolved problems are discussed.
文摘An F<sub>2</sub> population developed from the Xa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene, Xa-4. Some restriction fragment length polymorphism （RFLP） markers on the high-density map constructed by Harushima et al. and the amplified DMA fragments homologous to the conserved domains of plant disease resistance （R） genes were used to construct the genetic linkage map around the gene Xa-4 by scoring susceptible individuals in the population. Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction （PCR） marker M55. The R gene homologous fragment marker RS13 was found co-segregating with Xa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker RS13 can be applied to molecular marker-assisted selection of Xa-4 in rice breeding programs.
基金This work was supported by the Chinese 973 Program (Grant Nos. G1999011606 & G1999011604).
文摘Application and functional study of dwarf and semi-dwarf genes are of great importance to both crop breeding and molecular biology. A new semi-dwarf gene, sd-t(t), non-allelic to sd-1,had been identified in an indica rice variety, Aitaiyin 2. In this study the gene was genetically mapped by using an F2 population, which consisted of 474 individuals developed from a cross between Aitaiyin 2 and B30. The sd-t(t) gene was located between the RFLP markers R514 and R1408B with a distance of 1.1 cM to R514, and 4.5 cM to R1408B on chromosome 4. A physical contig covering the sd-t(t) mapping region was further constructed by screening a BAC library with R514 and R1408B as probes, and the physical distance between R514 and R1408B was estimated at approximately 147 kb. This result will facilitate map-based cloning of the sd-t(t) gene.
文摘西瓜炭疽病(Anthracnose)是由瓜类炭疽病菌(Colletotrichumorbiculare)引起的真菌性病害。本研究以抗病自交系PI189225和感病自交系Black Diamond杂交并自交获得F1、F2、F3为材料,采用炭疽病菌生理小种1接种,对西瓜抗炭疽病生理小种1进行遗传规律分析和基因定位研究。研究结果表明,西瓜炭疽病抗性基因由显性单基因控制,抗病对感病为显性,将此基因命名为Rco-1。用分离群体分组分析法(BSA)和AFLP分子标记技术对PI189225中的抗炭疽病基因进行分子标记鉴定,并利用MAPMAKER/Exp version 3.0软件进行了标记与目的基因间的遗传距离计算,发现E4/M19、E1/M8、E29/M5与抗炭疽病基因Rco-1连锁,遗传距离分别为34.8、23.4、6.9cM。为采用分子标记辅助选育抗炭疽病西瓜新品种奠定了基础。
