Background:Based on modern pharmacological studies,Terminalia chebula Retz.exhibits antimicrobial activity against a variety of bacteria.Previously,we found Terminalia chebula Retz.exhibited excellent antibacterial ac...Background:Based on modern pharmacological studies,Terminalia chebula Retz.exhibits antimicrobial activity against a variety of bacteria.Previously,we found Terminalia chebula Retz.exhibited excellent antibacterial activity against Malassezia restricta.Methods:We determined the minimal inhibitory concentration(MIC)and minimum bactericidal concentration of Terminalia chebula Retz.extraction with water(TRW)against Staphylococcus epidermidis(including Staphylococcus epidermidis 1-15)using the microdilution method.Staphylococcus epidermidis 1(SE11),which was the most sensitive to TRW,was selected as the test bacterium for subsequent experiments.The time-kill curve of TRW on SE11 was generated using the viable count method.Further,an in vitro biofilm model of SE11 was constructed using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide test,semi-quantitative crystal violet experiment,and scanning electron microscopy.The effects of TRW on the total amount of biofilm formation,the number of viable biofilm bacteria,and biofilm microstructure of SE11 were also determined using a semi-quantitative crystal violet experiment,viable count method,and scanning electron microscopy.Finally,the electrical conductivity and soluble protein content of the SE11 suspensions were determined.Results:The average MIC of TRW against SE11 was 0.75±1.09 mg/mL.TRW(1/2MIC and 2MIC)and zinc pyrithione(1/2MIC and 2MIC)had no significant effect on total biofilm inhibition in the adhesion stage(P>0.05)and the aggregation stage(P>0.05).Further,TRW(1/2MIC and MIC)and zinc pyrithione(1/2MIC and MIC)had no significant effect on viable biofilm bacteria in the adhesion stage(P>0.05)and aggregation stage(P>0.05).TRW destroyed the integrity of the SE11 cell membrane,resulting in leakage of intracellular substances.Conclusion:TRW inhibits SE11 biofilm formation and is similar to zinc pyrithione in the adhesion and aggregation stages,which provides a theoretical basis for its application in the field of antimicrobial additives.展开更多
对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均...对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均显示诃子粗提物具有很强的抗氧化活性。生物活性追踪结果发现,诃子抗氧化活性物质主要由存在于乙酸乙酯相的弱极性化合物组成,该相质量浓度为25、50μg/mL样品的总还原力OD700值分别为0.197±0.002和0.380±0.006,FRAP法抗氧化能力OD593分别为0.272±0.023和0.631±0.002,DPPH自由基清除率分别为(86.838±0.600)%和(90.318±0.917)%。相关关系表明,诃子抗氧化活性的主要物质基础为总黄酮和总多酚。展开更多
文摘Background:Based on modern pharmacological studies,Terminalia chebula Retz.exhibits antimicrobial activity against a variety of bacteria.Previously,we found Terminalia chebula Retz.exhibited excellent antibacterial activity against Malassezia restricta.Methods:We determined the minimal inhibitory concentration(MIC)and minimum bactericidal concentration of Terminalia chebula Retz.extraction with water(TRW)against Staphylococcus epidermidis(including Staphylococcus epidermidis 1-15)using the microdilution method.Staphylococcus epidermidis 1(SE11),which was the most sensitive to TRW,was selected as the test bacterium for subsequent experiments.The time-kill curve of TRW on SE11 was generated using the viable count method.Further,an in vitro biofilm model of SE11 was constructed using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide test,semi-quantitative crystal violet experiment,and scanning electron microscopy.The effects of TRW on the total amount of biofilm formation,the number of viable biofilm bacteria,and biofilm microstructure of SE11 were also determined using a semi-quantitative crystal violet experiment,viable count method,and scanning electron microscopy.Finally,the electrical conductivity and soluble protein content of the SE11 suspensions were determined.Results:The average MIC of TRW against SE11 was 0.75±1.09 mg/mL.TRW(1/2MIC and 2MIC)and zinc pyrithione(1/2MIC and 2MIC)had no significant effect on total biofilm inhibition in the adhesion stage(P>0.05)and the aggregation stage(P>0.05).Further,TRW(1/2MIC and MIC)and zinc pyrithione(1/2MIC and MIC)had no significant effect on viable biofilm bacteria in the adhesion stage(P>0.05)and aggregation stage(P>0.05).TRW destroyed the integrity of the SE11 cell membrane,resulting in leakage of intracellular substances.Conclusion:TRW inhibits SE11 biofilm formation and is similar to zinc pyrithione in the adhesion and aggregation stages,which provides a theoretical basis for its application in the field of antimicrobial additives.
文摘对诃子抗氧化活性物质提取工艺、抗氧化活性及其初步物质基础进行了研究。结果表明,最佳提取工艺为:乙醇体积分数50%、液料比20、提取温度60℃、提取次数4次、提取时间7 m in;总还原力、FRAP法抗氧化能力和DPPH自由基清除能力的结果均显示诃子粗提物具有很强的抗氧化活性。生物活性追踪结果发现,诃子抗氧化活性物质主要由存在于乙酸乙酯相的弱极性化合物组成,该相质量浓度为25、50μg/mL样品的总还原力OD700值分别为0.197±0.002和0.380±0.006,FRAP法抗氧化能力OD593分别为0.272±0.023和0.631±0.002,DPPH自由基清除率分别为(86.838±0.600)%和(90.318±0.917)%。相关关系表明,诃子抗氧化活性的主要物质基础为总黄酮和总多酚。
