期刊文献+
共找到1,651篇文章
< 1 2 83 >
每页显示 20 50 100
Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography 被引量:4
1
作者 Lei-Wen Xiang Jing Li +1 位作者 Jin-Ming Lin Hai-Fang Li 《Journal of Pharmaceutical Analysis》 SCIE CAS 2014年第2期153-158,共6页
Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in... Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaH2PO4 buffer solution at pH 5.26 (5:95). Good linearity between peak areas and concentrations of standards was obtained for the biomarkers with correlation coefficients in the range of 0.9957-0.9993. The proposed analytical method has satisfactory repeatability (the recovery of data in a range of creatinine, uric acid, hypoxanthine and xanthine was 93.49-97.90%, 95.38-96.45%, 112.46-115.78%and 90.82-97.13%with standard deviation of o5%, respectively) and the limits of detection (LODs, S/N Z 3) for creatinine, uric acid, hypoxanthine, and xanthine were 0.010, 0.025, 0.050 and 0.025 mg/L, respectively. The established method was proved to be simple, accurate, sensitive and reliable for the quantitation of gouty arthritis' biomarkers in human urine samples. The ratio of creatinine to uric acid was found to be a possible factor for assessment of gouty arthritis. 展开更多
关键词 Gouty arthritis CREATININE Uric acid HYPOXANTHINE XANTHINE high-performance liquid chromatography
下载PDF
Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor 被引量:2
2
作者 杨云梅 曹红翠 +1 位作者 徐哲荣 陈晓明 《Journal of Zhejiang University Science》 CSCD 2004年第6期738-742,共5页
Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart di... Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results:rHPLC is more convenient, sensitive,specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research. 展开更多
关键词 Platelet activating factor reversed phase high-performance liquid chromatographic technique Coronary heart disease Cerebral infarction
下载PDF
INTERSECTION POINT RULE OF THE RETENTION VALUE AND NORMAL BOILING POINT OF THE HOMOLOGUES IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY 被引量:1
3
作者 Fu An WANG Chang Sheng YANG +1 位作者 Wen Chang WANG Yuan Li JIANG 《Chinese Chemical Letters》 SCIE CAS CSCD 1991年第8期637-640,共4页
In this paper, a linear relationship between the logarithm of capacity factor k and normal boiling point to of the homologues has been derived, based on the basic retention equation of liquid chromatography according ... In this paper, a linear relationship between the logarithm of capacity factor k and normal boiling point to of the homologues has been derived, based on the basic retention equation of liquid chromatography according to statistical thermodyoamics proposed by professor Ln Peizhang and others, This equation has been verified by a large number of experimental data, all the strsight lines of lnk- of bumologues for different mobile phass coaiposltion cross each other at the same point, So the intereection point equation van proposed, wbich was used to prodict the retention valu, the result was satisfactory. 展开更多
关键词 BURR INTERSECTION POINT RULE OF THE RETENTION VALUE AND NORMAL BOILING POINT OF THE HOMOLOGUES IN reverseD-PHASE high-performance liquid chromatography
下载PDF
INTERSECTION POINT RULE OF THE RETENTION VALUE AND MOBILE PHASE COMPOSITION OF THE HOMOLOGUES IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY 被引量:1
4
作者 Fu An WANG Chang Sheng YANG +2 位作者 Yuan Li JIANG Deng Gao JIANG Da Zhuang LIU 《Chinese Chemical Letters》 SCIE CAS CSCD 1992年第11期907-910,共4页
Based on the intersection point rule of the retention value and normal boiling point of homologues in reversed-phase high-performance liquid chromatography(RPLC), the intersection point rule of the retention value of ... Based on the intersection point rule of the retention value and normal boiling point of homologues in reversed-phase high-performance liquid chromatography(RPLC), the intersection point rule of the retention value of homologues and mobile phase composition has been derived, and was testified by a lot of experimental data from the literature. With this newly proposed equation, we can use the retention value of the compound in one mobile phase composition to predict its retention value in any other mobile phase composition. For fourteen groups of homologues in five mobile phase compositions on five Kinds of columns, the overall average absolute error of 721 data sets is 2.8%. 展开更多
关键词 INTERSECTION POINT RULE OF THE RETENTION VALUE AND MOBILE PHASE COMPOSITION OF THE HOMOLOGUES IN reverseD-PHASE high-performance liquid chromatography
下载PDF
Quantitative analysis by reversed-phase high-performance liquid chromatography and retinal neuroprotection after topical administration of moxonidine
5
作者 Qian Zhang Mei-Fang Chu +5 位作者 Yan-Hong Li Chun-Hua Li Run-Jia Lei Si-Cen Wang Bao-Jun Xiao Jian-Gang Yang 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2020年第3期390-398,共9页
AIM:To determine moxonidine in aqueous humor and iris-ciliary body by reversed-phase high performance liquid chromatography(RP-HPLC),and to evaluate the retinal neuroprotective effect after topical administration with... AIM:To determine moxonidine in aqueous humor and iris-ciliary body by reversed-phase high performance liquid chromatography(RP-HPLC),and to evaluate the retinal neuroprotective effect after topical administration with moxonidine in a high intraocular pressure(IOP)model.METHODS:The eyes of albino rabbits were administered topically and ipsilaterally with 0.2%moxonidine.A RPHPLC method was employed for the identification and quantification of moxonidine between 2 and 480 min,which presented in the aqueous humor and iris-ciliary body.Flash electroretinography(F-ERG)amplitude and superoxide dismutase(SOD)level were measured between day 1 and day 15 after topical administration with moxonidine in a rabbit model of high IOP.Histological and ultrastructural observation underwent to analyze the changes of retinal morphology,the inner retinal layers(IRL)thickness,and retinal ganglion cell(RGC)counting.RESULTS:Moxonidine was detectable between 2 and 480 min after administration,and the peak concentration developed both in the two tissues at 30 min,0.51μg/m Lin aqueous humor and 1.03μg/g in iris-ciliary body.In comparison to control,F-ERG b-wave amplitude in moxonidine eyes were significantly differences between day 3 and day 15(P<0.01)in the high IOP model;SOD levels were significantly higher at all time-points(P<0.01)with a maximum level of 20.29 U/mgprot at day 15;and RGCs were significantly higher(P<0.05).CONCLUSION:Moxonidine is a viable neuroprotective agent with application to high IOP model.All layers of retina,including RGC layer,retinal nerve fiber layer and INL,are more preserved after moxonidine administration.SOD plays a neuroprotective role in ocular hypertension-mediated RGC death. 展开更多
关键词 reverseD-PHASE high-performance liquid chromatography MOXONIDINE RETINAL GANGLION cell NEUROPROTECTION superoxide DISMUTASE
下载PDF
A Sensitive Reversed-Phase High-Performance Liquid Chromatography Method for the Quantitative Determination of Milk Xanthine Oxidase Activity 被引量:1
6
作者 Zhongqin Li Ruizhang Guan Hongwei Liu 《Open Journal of Medicinal Chemistry》 2013年第1期26-30,共5页
A new reversed-phase high performance liquid chromatography method was developed to quantitate the activity of xanthine oxidase involved in milk fat globule membrane with xanthine as the substrate and the separation o... A new reversed-phase high performance liquid chromatography method was developed to quantitate the activity of xanthine oxidase involved in milk fat globule membrane with xanthine as the substrate and the separation of product (uric acid). The increment of uric acid in the reaction system was used to calculate the total activity of XO. The optimized assay conditions, linearity of detection, recovery of uric acid and chromatogram were developed in text, indicating this method is simple, rapid and efficient. It is an alternative potential method for the determination of the activity of XO in milk. 展开更多
关键词 XANTHINE OXIDASE (XO) Enzyme Activity Assay reverseD-PHASE High Performance liquid chromatography (RP-HPLC)
下载PDF
Development of a reversed dispersive based graphene functionalized with multiwalled carbon nanotubes for detection ofβ2-agonists in pork by high-performance liquid chromatography
7
作者 Ying-Wen Qian Xia Hong +3 位作者 Cai-Xia Yuan Xin-Kui Zhou Hai-Ning He Dong-Shuai Wang 《Food Science and Human Wellness》 SCIE 2018年第2期163-169,共7页
A reliable and inexpensive pretreatment procedure in the determination ofβ2-agonists in pork was developped.The procedure used a nanocomposite of multiwalled carbon nanotubes(CNTs)functionalized with graphene(rGO)as ... A reliable and inexpensive pretreatment procedure in the determination ofβ2-agonists in pork was developped.The procedure used a nanocomposite of multiwalled carbon nanotubes(CNTs)functionalized with graphene(rGO)as the reversed dispersive sorbent.It was analyzed after purification by highperformance liquid chromatography,the extraction time and the properties of the nanocomposite were optimized.Under optimized conditions,present method has linear response over concentration range of 0.5–50 ng/mL in pork samples with a satisfactory detection limit close to 0.1 ng/mL.The precisions of the current method(coefficient of variation)are lower than 5%,while recoveries are more than 98.3%.The nanocomposite exhibited high adsorptivity,long-term storage stability,satisfactory anti-interfering activity and high selectivity toward2-agonists compared with those of rGO and CNTs. 展开更多
关键词 high-performance liquid chromatography GRAPHENE Multiwalled carbon nanotube RACTOPAMINE SALBUTAMOL
下载PDF
High-Performance Liquid Chromatographical Analysis of Ginsenosides in Panax ginseng,P.quinquef(?)lium and P.notoginseng 被引量:1
8
作者 郎文生 楼之岑 毕培曦 《Journal of Chinese Pharmaceutical Sciences》 CAS 1993年第2期133-143,共11页
The compositions and contents of ginsenbsides in Panax ginseng,P.quinquefolium and P.notoginseng were determined and compared by reversed-phase High-Performance Liquid Chro- matography(HPLC).The method was performed o... The compositions and contents of ginsenbsides in Panax ginseng,P.quinquefolium and P.notoginseng were determined and compared by reversed-phase High-Performance Liquid Chro- matography(HPLC).The method was performed on an Alltech Adsorbosphere HS C_(18) column,using 5×10^(-3)M NaH_2PO_4-H_3PO_4 buffer solution(pH 3.0)and acetonitrile-water(50:50)as gradient eluents. The baseline separation of ginsenosides Rb_1,Rb_2,Rb_1,Rc,Rd,Rf,Ro,and Re+Rg_1 was obtained in one analytical run.The ginsenosides are directly detected at 203 nm.The detection limit is 40μg at a signal to noise ratio of 3:1.The improved sample preparation and clean-up prior to injection with SEP-PAK C_(18)cartridge strongly reduced the front peaks caused by the impurities in the methanolic extracts of samples to afford a smooth baseline and clear background.The HPLC patterns of methanolic extracts mainly including the ginsenosides were found capable of serving as chemical fingerprints to differentiate the three species from each other.It was also found that there are no significant diffe- rences of the HPLC patterns between the wild Panax ginseng and the cultivated,the white and the red ginsengs,Chinese and Korean red ginsengs,and the tap roots of Panax ginseng collected in four consecutive months,only certain differences in contents of ginsenosides do exist.The contents of the nine major ginsenosides present in the rhizome,tap root and rootlet as well as the leaf of Panax quinquefolium were also determined and compared. 展开更多
关键词 Panax ginseng Panax quinquefolium Panax notoginseng GINSENOSIDES high-performance liquid chromatography(HPLC)
下载PDF
Simultaneous determination of five nucleosides and nucleobases in Panax notoginseng using high-performance liquid chromatography 被引量:1
9
作者 王静 王一涛 李绍平 《Journal of Chinese Pharmaceutical Sciences》 CAS 2007年第2期79-83,共5页
Aim To quantitatively determine five nucleosides and nucleobases, including cytidine, uridine, guanosine, adenosine and uracil in different parts of Panax notoginseng. Methods Separation was performed on a Zorbax SB-A... Aim To quantitatively determine five nucleosides and nucleobases, including cytidine, uridine, guanosine, adenosine and uracil in different parts of Panax notoginseng. Methods Separation was performed on a Zorbax SB-Aq column using a gradient elution with mobile phase of 8 mmol^L-1 ammonium acetate aqueous solution (A) and methanol (B). The assay was carried out at a flow rate of 1 mL·min^-1 at 25 ℃ with the diode-array detection at 260 nm. Results Cytidine, uridine, guanosine, adenosine and uracil had good linearity in the ranges of 1.79 - 57.40 μg·mL^-1 (r^2 = 1.0000), 3.30 - 105.60 μg·mL^-1 (r^2 = 1.0000), 3.09 - 98.80 μg·mL^ -1(r^2 = 0.9999), 2.77 - 88.60 μg·mL^-1 (r^2 = 1.0000) and 0.38 - 12.30 μg·mL ^-1 (r^2 = 1.0000) with average recoveries of 93.9%, 96.5%, 92.7%, 93.2% and 98.8%, respectively. The content of cytidine, uridine, guanosine, adenosine and uracil in different parts of P. notogingeng were significantly different. Conclusion This is the first report on quantitative determination of nucleosides and nucleobases in P notoginseng. 展开更多
关键词 Panax notoginseng NUCLEOSIDE NUCLEOBASE high-performance liquid chromatography (HPLC)
下载PDF
Plasma free amino acid profiling of esophageal cancer using high-performance liquid chromatography spectroscopy 被引量:11
10
作者 Hong Ma Ayshamgul Hasim +3 位作者 Batur Mamtimin Bin Kong Hai-Ping Zhang Ilyar Sheyhidin 《World Journal of Gastroenterology》 SCIE CAS 2014年第26期8653-8659,共7页
AIM: To perform plasma free amino acid (PFAA) profiling of esophageal squamous cell carcinoma (ESCC) patients at different pathological stages and healthy subjects.
关键词 Metabolomics high-performance liquid chromatography Esophageal squamous cell cancer PLASMA Amino acids
下载PDF
Advancements in the preparation of high-performance liquid chromatographic organic polymer monoliths for the separation of small-molecule drugs 被引量:5
11
作者 Xiali Ding Jing Yang Yuming Dong 《Journal of Pharmaceutical Analysis》 SCIE CAS CSCD 2018年第2期75-85,共11页
The various advantages of organic polymer monoliths, including relatively simple preparation processes,abundant monomer availability, and a wide application range of pH, have attracted the attention of chromatographer... The various advantages of organic polymer monoliths, including relatively simple preparation processes,abundant monomer availability, and a wide application range of pH, have attracted the attention of chromatographers. Organic polymer monoliths prepared by traditional methods only have macropores and mesopores, and micropores of less than 50 nm are not commonly available. These typical monoliths are suitable for the separation of biological macromolecules such as proteins and nucleic acids, but their ability to separate small molecular compounds is poor. In recent years, researchers have successfully modified polymer monoliths to achieve uniform compact pore structures. In particular, microporous materials with pores of 50 nm or less that can provide a large enough surface area are the key to the separation of small molecules. In this review, preparation methods of polymer monoliths for high-performance liquid chromatography, including ultra-high cross-linking technology, post-surface modification, and the addition of nanomaterials, are discussed. Modified monolithic columns have been used successfully to separate small molecules with obvious improvements in column efficiency. 展开更多
关键词 high-performance liquid chromatography Polymer MONOLITH Preparation methods Small molecules
下载PDF
High-Performance Liquid Chromatographic Method for Determination of Germacrone in Rat Plasma 被引量:4
12
作者 HEHai-bing TANGXing CUIFu-de 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第3期190-192,共3页
Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV de... Aim A reliable high-performance liquid chromatographic (HPLC) method wasdeveloped for determination of germacrone in rat plasma. Methods The plasma samples were treatedwith acetonitrile and analyzed by HPLC with UV detection at 244 nm. Results The limit of detectionwas 100 ng·mL^(-1) for germacrone in plasma and the linear range was 0.1004-15.06 μg·mL^(-1) inplasma. The RSD of intra-day and inter-day assay was 1.87% - 4.29% and 1.29% -5.15%, respectively.The recoveries of germacrone were over 95%. The endogenous substances in plasma did not show anyinterference in the analysis. Conclusion The method is accurate and convenient, and suitable forpharmacokinetic studies of germacrone in rats. 展开更多
关键词 high-performance liquid chromatography GERMACRONE volatile oil of rhizomacurcumae
下载PDF
Dispersive Liquid-liquid Microextraction Combined with High-performance Liquid Chromatography for the Determination of Clozapine and Chlorpromazine in Urine 被引量:3
13
作者 陈静 熊朝梅 +1 位作者 阮金兰 苏邹 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2011年第2期277-284,共8页
A simple method has been proposed for the determination of clozapine (CLZ) and chlorpromazine (CPZ) in human urine by dispersive liquid-liquid microextraction (DLLME) in combination with high-performance liquid ... A simple method has been proposed for the determination of clozapine (CLZ) and chlorpromazine (CPZ) in human urine by dispersive liquid-liquid microextraction (DLLME) in combination with high-performance liquid chromatography-ultraviolet detector (HPLC-UV). All important variables influencing the extraction efficiency, such as pH, types of the extraction solvent and the disperser solvent and their volume, ionic strength and centrifugation time were investigated and optimized. Under the optimal conditions, the limit of detection (LODs) and quantification (LOQs) of the method were 13 and 39 ng/mL for CLZ, and 2 and 6 ng/mL for CPZ, respectively. The relative standard deviations (RSDs) of the targets were less than 5.1% (C=0.100 μg/mL, n=9). Good linear behaviors over the tested concentration ranges were obtained with the values of R20.999 for the targets. The absolute extraction efficiencies of CLZ and CPZ from the spiked blank urine samples were 98.3% and 97.8%, respectively. The applicability of the technique was validated by analyzing urine samples and the mean recoveries for spiked urine samples ranged from 93.3% to 105.0%. The method was successfully applied for the determination of CLZ and CPZ in real human urine. 展开更多
关键词 dispersive liquid-liquid microextraction CLOZAPINE CHLORPROMAZINE high-performance liquid chromatography human urine
下载PDF
Quantification of six bioactive compounds in Zhenqi Fuzheng preparation by high-performance liquid chromatography coupled with diode array detector and evaporative light scattering detector 被引量:4
14
作者 Yi-Kai Shi Fang Cui +3 位作者 Fang-Di Hu Ying-Yan Bi Yu-Feng Ma Shi-Lan Feng 《Journal of Pharmaceutical Analysis》 SCIE CAS 2011年第1期20-25,共6页
A simple and accurate high-performance liquid chromatography(HPLC)coupled with diode array detector(DAD)and evaporative light scattering detector(ELSD)was established for the determination of six bioactive compo... A simple and accurate high-performance liquid chromatography(HPLC)coupled with diode array detector(DAD)and evaporative light scattering detector(ELSD)was established for the determination of six bioactive compounds in Zhenqi Fuzheng preparation(ZFP).The monitoring wavelengths were 254,275 and 328 nm.Under the optimum conditions,good separation was achieved,and the assay was fully validated in respect of precision,repeatability and accuracy.The proposed method was successfully applied to quantify the six ingredients in 31 batches of ZFP samples and evaluate the variation by hierarchical cluster analysis(HCA),which demonstrated significant variations on the content of these compounds in the samples from different manufacturers with different preparation procedures.The developed HPLC method can be used as a valid analytical method to evaluate the intrinsic quality of this preparation. 展开更多
关键词 high-performance liquid chromatography(HPLC) diode array detector(DAD) evaporative light scattering detector(ELSD) Zhenqi Fuzheng preparation quantification hierarchical cluster analysis
下载PDF
Establishing a protein expression profile database for the normal human pituitary gland using two-dimensional high-performance liquid chromatography combined with LTQ-Orbitrap mass spectrometry 被引量:2
15
作者 Rong Xie Wei Xu +4 位作者 Weimin Bao Hang Liu Luping Chen Yiwen Shen Jianhong Zhu 《Neural Regeneration Research》 SCIE CAS CSCD 2012年第36期2922-2928,共7页
In this study, we selected adult normal pituitary gland tissues from six patients during operations for pituitary microadenomas via the transsphenoidal approach for extended normal pituitary tissue resection around th... In this study, we selected adult normal pituitary gland tissues from six patients during operations for pituitary microadenomas via the transsphenoidal approach for extended normal pituitary tissue resection around the tumor, and analyzed the protein expression of human normal pituitary using two-dimensional high-performance liquid chromatography combined with LTQ-Orbitrap mass spectrometry proteomics technology. The ten most highly expressed proteins in normal human pituitary were: alpha 3 type VI collagen isoform 5 precursor (abundance among tall pituitary proteins 1.30%), fibrinogen beta chain preproprotein (0.99%), vimentin (0.73%), prolactin (0.69%), ATP synthase, H~ transporting and mitochondrial F1 complex beta subunit precursor (0.52%), keratin I (0.49%), growth hormone (0.45%), carbonic anhydrase I (0.40%), heat shock protein 90 kDa I (0.31%), and annexin V (0.30%). Based on the biological function classifications of these proteins, the top three categories by content were neuroendocrine proteins (abundance among all pituitary proteins, 40.1%), catalytic and metabolic proteins (28.3%), and cell signal transduction proteins (9.8%). Based on cell positioning classification, the top three categories were cell organelle (24.5%) membrane (20.8%), and cytoplasm (13.0%). Based on biological process classification, the top three categories of proteins are involved in physiological processes (42.9%), cellular processes (40.4%), and regulation of biological processes (9.1%). Our experimental findings indicate that a protein expression profile database of normal human pituitary can be precisely and efficiently established by proteomics technology. 展开更多
关键词 two-dimensional high-performance liquid chromatography mass spectrum pituitary gland PROTEINS PROTEOMICS hypophyseal tumor physiological function pathological mechanism
下载PDF
Studies on Chromatography Fingerprint of Hongqi by High-performance Liquid Chromatography 被引量:2
16
作者 ShiLanFENG FangDiHU +2 位作者 JianXiongZHAO JingWenXU LiRenCHEN 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第11期1335-1338,共4页
Chromatography fingerprint (CFP) of 10 samples of hongqi were studied. 23 common peaks were analyzed, their average similarity was 97.29%. CFP were positioned with main index composition such as formononetin, calyco... Chromatography fingerprint (CFP) of 10 samples of hongqi were studied. 23 common peaks were analyzed, their average similarity was 97.29%. CFP were positioned with main index composition such as formononetin, calycosin and then the contents of index composition were determined. The character and exclusive of CFP of 10 samples of hongqi were clear. CFP and content determination of index composition of hongqi could be used to evaluate the quality of hongqi comprehensively. 展开更多
关键词 Hongqi chromatography fingerprint high-performance liquid chromatography.
下载PDF
Determination of 5-Fluorouracil in Human Plasma by High-Performance Liquid Chromatography (HPLC) 被引量:2
17
作者 谷元 陆榕 +1 位作者 司端运 刘昌孝 《Transactions of Tianjin University》 EI CAS 2010年第3期167-173,共7页
5-Fluorouracil (5-FU) has a broad spectrum of anti-tumor activity, widely applied to the treatment of cancers. However, it is necessary to determine the plasma concentration of 5-FU in clinical practice due to its nar... 5-Fluorouracil (5-FU) has a broad spectrum of anti-tumor activity, widely applied to the treatment of cancers. However, it is necessary to determine the plasma concentration of 5-FU in clinical practice due to its narrow therapeutic index. Therefore, a simple, economic and sensitive high-performance liquid chromatography (HPLC) method was developed and validated for the determination of 5-FU in human plasma. Ethyl acetate was chosen as extraction reagent. Chromatographic separation was performed on a Diamonsil C18 column (250 mm × 4.6 mm i.d., 5 μm) with the mobile phase consisting of methanol and 20 mmol/L ammonium formate using a linear gradient elution at a flow rate of 0.8 mL/min. 5-FU and 5-bromouracil (5-BU) were detected by UV detector at 265 nm. The calibration curve was linear over the concentration range of 5—500 ng/mL and the correlation coefficient was not less than 0.992 6 for all calibration curves. The intra- and inter-day precisions were less than 10.5% and 4.3%, respectively, and the accuracy was within ±3.7%. The recovery at all concentration levels was 80.1±8.6%. 5-FU was stable under possible conditions of storing and handling. This method is proved applicable to therapeutic drug monitoring and pharmacokinetic studies of 5-FU in human. 展开更多
关键词 5-fluorouracil (5-FU) high-performance liquid chromatography (HPLC) human plasma
下载PDF
Biological Fingerprinting Analysis of Interaction Between Taxoids in Taxus and Microtubule Protein by Microdialysis Coupled with High-performance Liquid Chromatography/Mass Spectrometry for Screening Antimicrotubule Agents 被引量:1
18
作者 LEI Xiao-yuan KONG Liang +2 位作者 SU Xing-ye GUO Ming ZOU Han-fa 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第4期411-419,共9页
Some natural products, such as traditional Chinese medicines(TCMs), contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. A quick and con... Some natural products, such as traditional Chinese medicines(TCMs), contain compounds with anticancer activity and have attracted a great interest in recent years as alternative anticancer therapies. A quick and convenient assay for screening antimicrotubule compounds in which in vitro microdialysis/high-performance liquid chromatography (HPLC) is used to monitor the binding of the compounds extracted from TCM Taxus cuspidata Siebold & Zucc(Taxus) to microtubules is reported. It was observed that the extract of Taxus contains at least five compounds which have affinity interaction with microtubules by biological fingerprinting analysis, and they were identified as the taxoids of taxol, baccatin III, 10-deacetylbaccatin Ⅲ(10-DAB), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT) based on the comparison of their high-performance liquid chromatographic/mass spectrometric and UV spectra with those of the standard samples, both assembly-promoting and disassembly-inhibiting characteristics of those compounds were evaluated. It was observed that baccatin Ⅲ and 10-DAB bound to microtubules and the binding degrees were influenced by GTP. Competitive binding behavior of taxol with other four taxoids to microtubules was also investigated. 展开更多
关键词 Microdialysis/high-performance liquid chromatography(HPLC) Biological fingerprinting analysis TAXOIDS MICROTUBULE TAXUS
下载PDF
High-performance Liquid Chromatographic Determination of Urinary Trans,Trans-Muconic Acid Excreted by Workers Occupationally Exposed to Benzene
19
作者 XIA-MIN HU SHI-ZHEN SONG +1 位作者 FANG-LI YE LI-WEN LIU 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2006年第4期292-296,共5页
Objective To investigate the relationship between trans, trans-muconic acid (ttMA) as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liqui... Objective To investigate the relationship between trans, trans-muconic acid (ttMA) as benzene metabolite of occupational workers and benzene concentration in air. Methods A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS (2) (5u) column (Ф4.6 min× 150 mm) and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water (v/v, 1:2:10:87). The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals. Results A correlation coefficient (r = 0.9963 ) was found for ttMA ranging 0.10-10.00 μg/mL. The limit of detection was 0.10μ g/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y=0.859+0.108C (before work, r=0.6200) or Y=1.980+0.179C (after work, r=0.7930). Conclusion This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene. 展开更多
关键词 BENZENE TRANS Trans-muconic acid high-performance liquid chromatography
下载PDF
Determination of Gastrodin Content in Gastrodia elata in Bomi of China by High-Performance Liquid Chromatography
20
作者 XIAO Guo-xin ZHAO Shui-ling +3 位作者 XIE Li-ling HAO Hai-li ZHU Yan-kun CHENG Kai 《Animal Husbandry and Feed Science》 CAS 2011年第5期40-41,44,共3页
[Objective] To select excellent varieties of Gastrodia elata and to determine the most suitable picking season of the plant. [Method] The gastrodin contents in fresh wild G. elata harvested in winter in Bomi County, a... [Objective] To select excellent varieties of Gastrodia elata and to determine the most suitable picking season of the plant. [Method] The gastrodin contents in fresh wild G. elata harvested in winter in Bomi County, artificially cultivated fresh G. elata harvested in winter in Bomi County, and nonlocal G. elata were determined by high-performance liquid chromatography (HPLC) under the following conditions., chromato- graphic column, AngilentTc-C18 (5 μm, 4.6 mm × 250.0 mm) ; mobile phase, acetonitrile-water (5:95, V/V) ; wavelength, 220 nm; flow rate, 1.0 ml/min; column temperature, 35℃; and injection volume, 10 μl. The gastrodin contents in G. elata respectively harvested in winter and summer were compared. [ Result] The gastrodin content was higher in the fresh wild G. elata harvested in winter in Bomi County (0.076%) than in the artificially cultivated fresh G. elata harvested in winter in Bomi County (0.045%) and nonlocal G. elata (0.025%). Additionally, the gastrodin content was higher in G. elata harvested in winter than in those harvested in summer. [ Conclusion] G. elata from Bomi Country is an excellent variety and should be harvested in winter. 展开更多
关键词 Gastrodia elata Bomi County GASTRODIN high-performance liquid chromatography
下载PDF
上一页 1 2 83 下一页 到第
使用帮助 返回顶部