Glutathione Peroxidase Revisited—Simulation of the Catalytic Cycle by Computer-Assisted Molecular Modelling

Glutathione peroxidase, the first example of selenoproteins identified in mammals, was subjected to force field calculations and molecular dynamics in order to enable a clearer comprehension of enzymatic selenium catalysis. Starting from the established X-ray structure of bovine GPX, all kinetically defined intermediates and enzyme substrate complexes were modelled. The models thus obtained support the hypothesis that the essential steps of the catalysis are three distinct redox changes of the active site selenium which, in the ground state, presents itself at the surface of selenoperoxidases as the center of a characteristic triad built by selenocysteine, glutarnine and tryptophan. In GPX, four arginine residues and a lysine residue provide an electrostatic architecture which, in each reductive step, directs the donor substrate GSH towards the catalytic center in such a way that 1ts sulfhydryl group must react with the selenium moiety. To this end, different equally efficient modes of substrate binding appear possible. The models are consistent with substrate specificity data, kinetic pattern and other functional characteristics of the enzyme. Comparison of molecular models of GPX with those of other members of the GPX superfamily reveals that the cosubstrate binding mechanisrns are unique for the classical type of cytosolic glutathione peroxidases but cannot operate e. g. in plasma GPX and phospholipid hydroperoxide GPX. The structural differences between the selenoperoxidases, shown to be relevant to their specificities, are discussed in terms of functional diversification within the GPX superfamily

Block-Based Adaptive Image Steganography Using LSB Matching Revisited

Recently, an edge adaptive image stegano- graphic method based on least significant bit （LSB） matching revisited （EA-LSBMR） has been proposed, which holds good visual quality and proper security under appropriate embedding rates. However, from the extensive experiments to EA-LSBMR, we find that the discrete Fourier transform （DFT） spectrum of pixelpairs differences histogram still reveals the presence of a secret message even in a low embedding rate. To enhance the security, a modified scheme is proposed in this paper, which can defeat the above-mentioned analysis and keep the visual quality better than EA-LSBMR in higher embedding rates. Experimental results using a latest universal steganalysis method have demonstrated the proposed method＇s good performance.

A Character Analysis of Charlie in Fitzgerald's Babylon Revisited

Babylon Revisited is one of Fitzgerald's greatest stories. In this story, the protagonist Charlie comes to Paris to get custody of her daughter Honoria from his wife's sister Marion, to whom the child is entrusted after Charlie fell apart several years ago. However, he is prevented by his late wife's sister Marion for her selfishness and jealousy. Perhaps, Fitzgerald wants us to question whether Charlie is really prepared for parenting. The story hints that he isn't, because it provides many hints that he has not separated himself enough from his past bad habits.

Precambrian Dykes in the Sao Francisco Craton Revisited:Geochemical-isotopic Signatures and Tectonic Significance

Several generations of mafic dyke swarms(2.7 to 0.8Ga),highlighted by aeromagnetic data,petrography,geochemistry,isotope geology and mostly U-Pb baddeleyite ages,crosscut the Archean and

Dual Image Reversible Data Hiding by Modifying Least Significant-Bit Matching Revisited Rules

The concept of dual image reversible data hiding（DIRDH） is the technique that can produce two camouflage images after embedding secret data into one original image.Moreover,not only can the secret data be extracted from two camouflage images but also the original image can be recovered.To achieve high image quality,Lu et al.＇s method applied least-significant-bit（LSB） matching revisited to DIRDH.In order to further improve the image quality,the proposed method modifies LSB matching revisited rules and applies them to DIRDH.According to the experimental results,the image quality of the proposed method is better than that of Lu et al.＇s method.

Nyingchi Revisited

In1951,When Tibet wonpeaceful liberation, PLAsoldiers reached a townwith only a few house-holds. As a token of thanks forthe PLA who strove to expandit, the town was named Bayi,or August 1,the day on whichthe People’s Liberation Armywas founded.

Ancient Art Form Revisited

Chinese New Year painting exhibition reveals the beauty of folk art A CHINESE New Year painting exhibition has offered thousands of art lovers a traditional folk art feast in Beijing this past January and February.With the theme of "Door Gods," over 300 works of art were on display,representing various styles in China’s different regions. Woodcut prints,each a piece of special handmade folk art featuring auspicious subjects,are characterized by their concise lines, bright colors,and joyful scenes embodying people’s best wishes for the New Year.

哲学与翻译的互文——从《问题式、症候阅读与意识形态》到Althusser Revisited

本文从互文性理论出发,对中国经典哲学著作《问题式、症候阅读与意识形态》的互文性特点和英译策略进行了深入探讨。研究发现,原作的互文性与原作的"问题式"和"症候阅读"等概念相呼应,具体体现在超文本性与拼贴两个层面。而在翻译过程中,译者也从互文性出发,运用并置、调整、变异、融合与持存等翻译策略力求在语词、语言形态、文本色彩等多方面呈现原作的互文特性与哲学风貌,从而为哲学著作的创作、翻译和接受提供了新的模式和方法,彰显了译作与原作的互文关系。

Nanjing Revisited

New movie shows the pain of the Japanese invasion from a fresh perspective The Nanjing Massacre still weighs heavy on the Chinese psyche.

Balletic Violence Revisited

John Woo returns to his signature"gun fu"action thrillersIt has been 50 years since John Woo,the renowned Hong Kong director,started his career in the film industry back in 1968.Woo,now aged 71,has staged more than 30f ilms throughout an illustrious career which has spanned both Hong Kong and Hollywood,winning applause both at home and abroad for his distinctive style of action movies.This winter saw two of Woo’s f ilms

Lady Zhang Fuqing: Her Life, Family and Work Revisited

ZHANG Fuqing, born in June, 1919, was from Fenyang, Shanxi Province. During her childhood, she went to school in Beijing, where her knowledge was broadened and her field of vision was widened. She gradually grew into a talented and sedate girl. It was while she was still a little girl that she developed an interest in cooking. At home she often helped her parents to prepare meals. On New Year’s Day or during other festivals, what interested

Community Authenticity Experience of Shared Accommodation Consumers

By constructing a research framework of theoretical concepts such as leisure involvement, authenticity experience, place attachment, and willingness to revisit, the aim is to explore the authenticity experience and its influencing mechanisms in shared accommodation consumption. Research data are collected by using questionnaire survey method, and statistical analysis of data is conducted using SPSS 21.0. Research has found that leisure involvement has a significant positive impact on authenticity experience, place attachment, and willingness to revisit;authenticity experience has a significant positive impact on place attachment and willingness to revisit;place attachment has a significant positive impact on willingness to revisit. Therefore, shared accommodation owners need to strengthen the editing and publishing of leisure strategies on we media to enhance customers sense of leisure involvement;pay attention to enhancing the local authentic cultural experience of tenants, in order to increase their sense of attachment to the place and thereby enhance their willingness to stay.

The Impact of Destination Image on Tourist Willingness to Revisit:A Case Study of Nanning Garden Expo

The researcher aims to examine the impact of destination image of Nanning Garden Expo on tourist willingness to revisit.In this study,Nanning Garden Expo,a famous comprehensive park in Nanning,Guangxi,is selected as the research object.Based on previous literature and theories,the author analyzes and explores the relationship between the destination image of Nanning Garden Expo and tourist willingness to revisit.The research tool is questionnaire and the sample size of the survey data is calculated by Cochrane formula,and the data collection volume is 385.The statistics used in the data analysis are descriptive statistics including frequency distribution,percentage,mean,standard deviation,and inferential statistic for testing hypothesis.The researcher applies multiple regression analysis models to examine which independent variables have strong or weak relationships with dependent variables.Hypothesis test indicates that destination image has a significant positive impact on tourist willingness to revisit.Based on the results of the empirical study,relevant suggestions are proposed for how to enhance tourist willingness to revisit for Nanning Garden Expo and promote the healthy and sustainable development of tourism.

Human parvovirus B19-associated hematopathy in HIV disease:need for clinicopathological revisit

Persons living with HIV infection occasionally suffer from anemia due to varying causes.These include the use of zidovudine,malnutrition especially vitamin B12and iron deficiency,opportunistic infections by Mycobacterium tuberculosis,Pneumocystis jiroveci,and direct hematological effects of HIV infection itself within the marrow microenvironment.Persistent Parvovirus B19（B19V）infection is a clinically important and treatable etiology of anemia in HIV-infected persons.

Revisiting the Electron-Doped SmFeAsO:Enhanced Superconductivity up to 58.6 K by Th and F Codoping

In the iron-based high-To bulk superconductors, Tc above 50 K was only observed in the electron-doped 1111-type compounds. Here we revisit the electron-doped SmFeAsO polycrystals to make a further investigation for the highest Tc in these materials. To introduce more electron carriers and less crystal lattice distortions, we study the Th and F eodoping effects into the Sm-O layers with heavy electron doping. Dozens of Sm1-xThx FeAsO1-yFy samples are synthesized through the solid state reaction method, and these samples are carefully characterized by the structural, resistive, and magnetic measurements. We find that the codoping of Th and F clearly enhances the superconducting Tc more than the Th or F single-doped samples, with the highest record Tc up to 58.6K when x = 0.2 and y=0.225. Further element doping causes more impurities and lattice distortions in the samples with a weakened superconductivity.

A revisit of rod microglia in preclinical studies

Microglial cells（or microglia）are the mononuclear phagocytes residing in the central nervous system（CNS）.In homeostasis,they showed ramified morphology with relative small cell bodies and long processes（Figure 1A）.They detect injury signals in the CNS and get activated.

Exploring Tourism Risk at Jaffna Peninsula with Special Reference to Keerimalai Ritual Beach Site in Sri Lanka

Ritual beach sites are considered as one of the major travel motives for the tourists to visit Sri Lanka.Jaffna is rich with wonderful ritual beach sites including Keerimalai and Villunri areas.Many local and foreign tourists arrive at these ritual beach sites every day.Ensuring safety of tourists in the tourist destinations is considered as one of the important factors which determine the revisit and for remaining the higher number of tourist arrivals.But,the studies about the risks for tourists at the destinations,especially in the ritual beach sites,were very rarely conducted by the researchers.Therefore,a study to explore the tourism risks at the ritual beach site was considered very important.Further,if the tourists feel that the particular destination is risky,the image of the destination shall be affected and also tourists may negatively convey the message to others.Therefore,the main objective of this study is to identify the factors influencing the tourism risk in Jaffna Keerimalai ritual sea site.This study also recommends overcoming destination risks to ensure propensity of the repeat visit and word of mouth communication.For this purpose,the study was carried out by using the mix research methodology.Both qualitative and quantitative methods were used to carry out the study.Furthermore,the Keerimalai ritual beach site was selected through the convenience sample technique.Primary data were collected through self-administered questionnaires and the observation techniques.The 5-point Likert scale method was used to analyze the data through SPSS Version 20.This study revealed that Keerimalai ritual sea site has risk for tourists and this may affect revisit and word of positive recommendation.Alcohol addiction,theft activity,violations,rocks under the sea water,safety and security signs and polluted water are harmful to the tourist.In spite of all these risks,there are many tourists who still visit Keerimalai ritual area to accomplish their cultural rituals.

Revisiting the Places Where Liang Sicheng and Lin Huiyin Investigated in Shanxi Province

The wall is the epitome of the ancient society,every single brick in the wall retains the information and culture of that era.