The present study utilized samples from bilateral motor branches of the femoral nerve, as well as saphenous nerves, ventral roots, and dorsal roots of the spinal cord, to detect differential protein expression using t...The present study utilized samples from bilateral motor branches of the femoral nerve, as well as saphenous nerves, ventral roots, and dorsal roots of the spinal cord, to detect differential protein expression using two-dimensional gel electrophoresis and nano ultra-high performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry techniques. A mass spectrum was identified using the Mascot search. Results revealed differential expression of 11 proteins, including transgelin, Ig kappa chain precursor, plasma glutathione peroxidase precursor, an unnamed protein product （gil55628）, gfyceraldehyde-3-phosphate dehydrogenase-like protein, lactoylgfutathione lyase, adenyfate kinase isozyme 1, two unnamed proteins products （gil55628 and gi11334163）, and poly（rC）-binding protein 1 in motor and sensory nerves. Results suggested that these proteins played roles in specific nerve regeneration following peripheral nerve injury and served as specific markers for motor and sensory nerves.展开更多
Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional g...Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional gel electrophoresis （2-DE） and mass spectrometry were used to separate and identify differentially expressed salivary gland proteins between the fed and unfed female R. haemaphysaloides. The soluble proteins from the salivary glands of fed and unfed female R. haemaphysaloides were separated by a sequential extraction method followed by 2-DE and 2-DE images. Image analysis of the gels revealed 1 096 ± 87 protein spots from the fed female ticks and 991 ±64 protein spots from the unfed female ticks. Among those protein spots, about 724 ±34 were present both in the fed and unfed female ticks. Fourteen spots from the fed ticks and six spots from the unfed ticks were selected for peptide mass fingerprinting （PMF） and sequencing assay by mass spectrometry （MS）. Bioinformatic analysis showed that a majority of the differentially expressed proteins were involved in signal transduction, metabolism, and transcriptional regulation. These differentially expressed proteins might be antigen candidates for the development of vaccines against the tick.展开更多
基金supported by the Key Projects in the National Science & Technology Pillar Program, No.2009BAI87B02the National Natural Science Foundation of China, No. 31100696the National Basic Research Program of China (973 Program), No. 2012CB518106
文摘The present study utilized samples from bilateral motor branches of the femoral nerve, as well as saphenous nerves, ventral roots, and dorsal roots of the spinal cord, to detect differential protein expression using two-dimensional gel electrophoresis and nano ultra-high performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry techniques. A mass spectrum was identified using the Mascot search. Results revealed differential expression of 11 proteins, including transgelin, Ig kappa chain precursor, plasma glutathione peroxidase precursor, an unnamed protein product （gil55628）, gfyceraldehyde-3-phosphate dehydrogenase-like protein, lactoylgfutathione lyase, adenyfate kinase isozyme 1, two unnamed proteins products （gil55628 and gi11334163）, and poly（rC）-binding protein 1 in motor and sensory nerves. Results suggested that these proteins played roles in specific nerve regeneration following peripheral nerve injury and served as specific markers for motor and sensory nerves.
基金supported by the National High Technology Research and Development Program of China(2006AA 10A207)the Basic Research Foundationfor National Commonweal Institute of China(2006JB02)
文摘Identification of differentially expressed salivary gland proteins between the fed and unfed female Rhipicephalus haemaphysaloides may obtain valuable functional molecules. In this study, comparative two-dimensional gel electrophoresis （2-DE） and mass spectrometry were used to separate and identify differentially expressed salivary gland proteins between the fed and unfed female R. haemaphysaloides. The soluble proteins from the salivary glands of fed and unfed female R. haemaphysaloides were separated by a sequential extraction method followed by 2-DE and 2-DE images. Image analysis of the gels revealed 1 096 ± 87 protein spots from the fed female ticks and 991 ±64 protein spots from the unfed female ticks. Among those protein spots, about 724 ±34 were present both in the fed and unfed female ticks. Fourteen spots from the fed ticks and six spots from the unfed ticks were selected for peptide mass fingerprinting （PMF） and sequencing assay by mass spectrometry （MS）. Bioinformatic analysis showed that a majority of the differentially expressed proteins were involved in signal transduction, metabolism, and transcriptional regulation. These differentially expressed proteins might be antigen candidates for the development of vaccines against the tick.
