目的采用序列特异性引物PC R技术(PC R-SSP)进行R H D基因定型。方法采用新近报道的PC R-SSP R H D定型方法检测10例R hD阳性个体,包括2例哈萨克族人、2例蒙古族人、2例藏族人和4例维吾尔族人,以及11例R hD阴性个体(汉族10例、维吾尔族...目的采用序列特异性引物PC R技术(PC R-SSP)进行R H D基因定型。方法采用新近报道的PC R-SSP R H D定型方法检测10例R hD阳性个体,包括2例哈萨克族人、2例蒙古族人、2例藏族人和4例维吾尔族人,以及11例R hD阴性个体(汉族10例、维吾尔族人1例),并通过测序和内含子检测分析1例汉族个体的R H D基因型。结果全部样品的PC R-SSP检测结果与血清学表型相符,1例汉族R hD阴性个体鉴定为D放散型,携带R H D1227A等位基因,1例个体的检测结果提示携带融合R H D基因。测序分析显示该融合基因的第1、2和第10外显子序列与正常R H D基因相应外显子相同,而缺失其余外显子,由于R H D基因和R H C E(e)基因的第2外显子序列相同,笔者采用PC R方法检测R H D基因第2内含子,结果为阴性,说明该个体携带的融合基因为R H D-C E(2-9)-D2等位基因。结论PC R-SSP可用于中国人R h血型D抗原基因定型。展开更多
BACKGROUND: There are few data on blood group(BG) types and types of pancreatic cancers. The aims of this study were to study BG types and BG-antigens in pancreatic intraductal papillary mucinous neoplasms(IPMNs). MET...BACKGROUND: There are few data on blood group(BG) types and types of pancreatic cancers. The aims of this study were to study BG types and BG-antigens in pancreatic intraductal papillary mucinous neoplasms(IPMNs). METHODS: BG type and tumor BG-antigen(glycoprotein) expression(studied by immunohistochemistry on tissue microarrays) were analyzed with regard to characteristics of 101 surgically resected pancreatic IPMNs. RESULTS: Non-O BG type predicted invasive carcinoma independently from high serum CA19-9 and male gender. BG type A was observed more frequently in women than in men. Chronic pancreatitis was more frequently seen in patients with BG type B or AB. Aberrant tumor expression(with regard to BG type) of loss of A antigen expression type occurred in 15.0% of IPMNs and of loss of B antigen expression type in 62.5% of IPMNs. Intraneoplasm BG-antigen expression was not related to dysplasia grade or invasion. CONCLUSION: The results of the study suggest that in pancreatic IPMN, non-O BG type predicted invasive carcinoma, whereas for intratumor BG-antigen expression no specific patterns were detected with regard to the progression of glandular epithelial dysplasia or invasion.展开更多
文摘目的采用序列特异性引物PC R技术(PC R-SSP)进行R H D基因定型。方法采用新近报道的PC R-SSP R H D定型方法检测10例R hD阳性个体,包括2例哈萨克族人、2例蒙古族人、2例藏族人和4例维吾尔族人,以及11例R hD阴性个体(汉族10例、维吾尔族人1例),并通过测序和内含子检测分析1例汉族个体的R H D基因型。结果全部样品的PC R-SSP检测结果与血清学表型相符,1例汉族R hD阴性个体鉴定为D放散型,携带R H D1227A等位基因,1例个体的检测结果提示携带融合R H D基因。测序分析显示该融合基因的第1、2和第10外显子序列与正常R H D基因相应外显子相同,而缺失其余外显子,由于R H D基因和R H C E(e)基因的第2外显子序列相同,笔者采用PC R方法检测R H D基因第2内含子,结果为阴性,说明该个体携带的融合基因为R H D-C E(2-9)-D2等位基因。结论PC R-SSP可用于中国人R h血型D抗原基因定型。
文摘BACKGROUND: There are few data on blood group(BG) types and types of pancreatic cancers. The aims of this study were to study BG types and BG-antigens in pancreatic intraductal papillary mucinous neoplasms(IPMNs). METHODS: BG type and tumor BG-antigen(glycoprotein) expression(studied by immunohistochemistry on tissue microarrays) were analyzed with regard to characteristics of 101 surgically resected pancreatic IPMNs. RESULTS: Non-O BG type predicted invasive carcinoma independently from high serum CA19-9 and male gender. BG type A was observed more frequently in women than in men. Chronic pancreatitis was more frequently seen in patients with BG type B or AB. Aberrant tumor expression(with regard to BG type) of loss of A antigen expression type occurred in 15.0% of IPMNs and of loss of B antigen expression type in 62.5% of IPMNs. Intraneoplasm BG-antigen expression was not related to dysplasia grade or invasion. CONCLUSION: The results of the study suggest that in pancreatic IPMN, non-O BG type predicted invasive carcinoma, whereas for intratumor BG-antigen expression no specific patterns were detected with regard to the progression of glandular epithelial dysplasia or invasion.
