Rhabditis sp.Bn strain was the first time found suppressive on plant parasitic nematodes(PPNs)in the rhizosphere of greenhouse cucumber.The nematode Bn was applied into soil at the rate of 1.0×1010 Juveniles/hm2 ...Rhabditis sp.Bn strain was the first time found suppressive on plant parasitic nematodes(PPNs)in the rhizosphere of greenhouse cucumber.The nematode Bn was applied into soil at the rate of 1.0×1010 Juveniles/hm2 10 days before cucumber plants being planted in October 2004 and then once a month as treatment.The same volume of tap water was used as control.The density of PPNs increased sharply in control and reached 1 561 Js/100 mL soil in April,whereas it was less than 400 Js/100 mL soil in treatment,significantly lower than that of control(P<0.01).The densities of PPNs,Meloidogyne spp.and Tylenchorhynchus spp.were reduced by 70% and 87% respectively by applying Rhabditis sp.Bn strain.The root-knot index of cucumber in treatment was also reduced significantly(P<0.05)in April 2005.展开更多
The occurrence of entomopathogenic nematodes(EPNs)in arable soil samples from Nigeria was investigated using Baermann extraction tray and insect-bait(White’s trap)techniques.Isolates were tested for infectivity using...The occurrence of entomopathogenic nematodes(EPNs)in arable soil samples from Nigeria was investigated using Baermann extraction tray and insect-bait(White’s trap)techniques.Isolates were tested for infectivity using the larvae of Galleria mellonella(greater moth)and Tenebrio molitor(mealworm).The study revealed a new species of Heterorhabditis(MT371593)in soil samples that were randomly collected from an arable farmland cultivated with cassava TMS-30572 at the Teaching and Research Farm of Landmark University,Nigeria.Amplification of the internal transcribed spacer region(ITS)of the ribosomal DNA produced a nucleotide sequence of 933 base pairs(bp).A BLASTN search of GenBank showed that the sequence of the Nigerian isolate is identical at 99%similarity to that of Heterorhabditis sp.from Thailand.Infectivity test of the isolate showed 100%mortality against T.molitor larvae within 48 h of exposure while only 80%mortality was recorded for G.mellonella after 1 week of exposure.This is the first account of Heterorhabditis sp.in Nigeria.The varying degrees of infectivity against mealworm and greater moth observed in this study proved that the Nigerian isolate of Heterorhabditis sp.could potentially be an attractive option in the management of insect pests of cash crops.展开更多
文摘Rhabditis sp.Bn strain was the first time found suppressive on plant parasitic nematodes(PPNs)in the rhizosphere of greenhouse cucumber.The nematode Bn was applied into soil at the rate of 1.0×1010 Juveniles/hm2 10 days before cucumber plants being planted in October 2004 and then once a month as treatment.The same volume of tap water was used as control.The density of PPNs increased sharply in control and reached 1 561 Js/100 mL soil in April,whereas it was less than 400 Js/100 mL soil in treatment,significantly lower than that of control(P<0.01).The densities of PPNs,Meloidogyne spp.and Tylenchorhynchus spp.were reduced by 70% and 87% respectively by applying Rhabditis sp.Bn strain.The root-knot index of cucumber in treatment was also reduced significantly(P<0.05)in April 2005.
基金The authors are grateful to the Management of Landmark University for financial assistance and to Prof.A.P.Malan of the Department of Conservation Ecology&Entomology,Stellenbosch University,South Africa for providing guidance with molecular identification.
文摘The occurrence of entomopathogenic nematodes(EPNs)in arable soil samples from Nigeria was investigated using Baermann extraction tray and insect-bait(White’s trap)techniques.Isolates were tested for infectivity using the larvae of Galleria mellonella(greater moth)and Tenebrio molitor(mealworm).The study revealed a new species of Heterorhabditis(MT371593)in soil samples that were randomly collected from an arable farmland cultivated with cassava TMS-30572 at the Teaching and Research Farm of Landmark University,Nigeria.Amplification of the internal transcribed spacer region(ITS)of the ribosomal DNA produced a nucleotide sequence of 933 base pairs(bp).A BLASTN search of GenBank showed that the sequence of the Nigerian isolate is identical at 99%similarity to that of Heterorhabditis sp.from Thailand.Infectivity test of the isolate showed 100%mortality against T.molitor larvae within 48 h of exposure while only 80%mortality was recorded for G.mellonella after 1 week of exposure.This is the first account of Heterorhabditis sp.in Nigeria.The varying degrees of infectivity against mealworm and greater moth observed in this study proved that the Nigerian isolate of Heterorhabditis sp.could potentially be an attractive option in the management of insect pests of cash crops.
