Objectives To investigate the gene expression of calcium - handling proteins in patients with rheumatic heart disease (RHD) and atrial fibrillation (AF) . Methods A total of 50 patients with rheumatic mitral valve dis...Objectives To investigate the gene expression of calcium - handling proteins in patients with rheumatic heart disease (RHD) and atrial fibrillation (AF) . Methods A total of 50 patients with rheumatic mitral valve disease were included. According to cardiac rhythm and duration of episode of AF, patients were divided into four groups: sinus rhythm group, paroxysmal AF group, persistent AF for less than 6 months group and persistent AF for more than 6 months group. Atrial tissue was obtained from the right atrial appendage, the right atrial free wall and the left atrial appendage respectively during open heart surgery. Total RNA was isolated and reversly transcribed into cDNA. In a semi - quantitative polymerase chain reaction the cDNA of interest and of glyceralde-hyde3 - phosphate dehydrogenase (GAPDH) were amplified and separated by ethidium bromide - stained gel electrophoresis. Multiple liner regress was used for correlation between the mRNA amount and age, sex, right atrial diameter (RAd) and left atrial diameter (LAd) . Results The mRNA of L - type calcium channeled subunit, of Ca2+ - ATPase and of ryanodine receptor in patients with persistent AF for more than 6 months were significantly decreased ( P all < 0. 01) . But no alterations of the mRNA levels for SR phos-pholamban and calsequestrin were observed in patients with persistent AF for more than 6 months compared with patients with sinus rhythm, paroxysmal AF and persistent AF for less than 6 months ( P all > 0. 05) . There was no difference of the gene expression among the three atrial tissue sampling sites (P all > 0. 05) . Age, gender, RAd and LAd had no significant effects on the gene expression of calcium - handling proteins (P all>0. 05). Conclusions The mRNA expression of calcium - handling proteins is down - regulated only in patients with RHD and long - term persistent AF. Such abnormalities may be related to the initiation and/or perpetuation of AF in the patients with RHD.展开更多
目的研究风湿性心脏病合并慢性心房颤动心房肌中FHL2蛋白水平的变化,探讨FHL2改变在慢性心房颤动中的作用。方法按性别、年龄、二尖瓣狭窄程度、左房大小及肺动脉压力配对,收集接受瓣膜置换手术的风湿性二尖瓣狭窄患者共28例(窦性组14例...目的研究风湿性心脏病合并慢性心房颤动心房肌中FHL2蛋白水平的变化,探讨FHL2改变在慢性心房颤动中的作用。方法按性别、年龄、二尖瓣狭窄程度、左房大小及肺动脉压力配对,收集接受瓣膜置换手术的风湿性二尖瓣狭窄患者共28例(窦性组14例,房颤组14例)。在建立体外循环时采取右心耳组织及部分慢性心房颤动患者左心耳组织(7例),用Western blot法检测FHL2蛋白在心房肌中的表达水平。结果在右心耳水平,房颤组FHL2蛋白(0.68±0.28 vs 0.51±0.18,t=2.488,P=0.027)表达水平较窦性组明显上调。房颤组FHL2蛋白的表达量在右心耳与左心耳差异无统计学意义(0.58±0.22 vs 0.50±0.13,t=0.984,P=0.363)。结论 FHL2蛋白在风湿性心脏病合并慢性心房颤动心房肌中表达上调,其可能参与慢性心房颤动的发生及维持。而FHL2蛋白在慢性心房颤动左、右心房肌的表达无明显差异,表明右心耳标本替代左心耳在本研究中的结果是可信的。展开更多
文摘Objectives To investigate the gene expression of calcium - handling proteins in patients with rheumatic heart disease (RHD) and atrial fibrillation (AF) . Methods A total of 50 patients with rheumatic mitral valve disease were included. According to cardiac rhythm and duration of episode of AF, patients were divided into four groups: sinus rhythm group, paroxysmal AF group, persistent AF for less than 6 months group and persistent AF for more than 6 months group. Atrial tissue was obtained from the right atrial appendage, the right atrial free wall and the left atrial appendage respectively during open heart surgery. Total RNA was isolated and reversly transcribed into cDNA. In a semi - quantitative polymerase chain reaction the cDNA of interest and of glyceralde-hyde3 - phosphate dehydrogenase (GAPDH) were amplified and separated by ethidium bromide - stained gel electrophoresis. Multiple liner regress was used for correlation between the mRNA amount and age, sex, right atrial diameter (RAd) and left atrial diameter (LAd) . Results The mRNA of L - type calcium channeled subunit, of Ca2+ - ATPase and of ryanodine receptor in patients with persistent AF for more than 6 months were significantly decreased ( P all < 0. 01) . But no alterations of the mRNA levels for SR phos-pholamban and calsequestrin were observed in patients with persistent AF for more than 6 months compared with patients with sinus rhythm, paroxysmal AF and persistent AF for less than 6 months ( P all > 0. 05) . There was no difference of the gene expression among the three atrial tissue sampling sites (P all > 0. 05) . Age, gender, RAd and LAd had no significant effects on the gene expression of calcium - handling proteins (P all>0. 05). Conclusions The mRNA expression of calcium - handling proteins is down - regulated only in patients with RHD and long - term persistent AF. Such abnormalities may be related to the initiation and/or perpetuation of AF in the patients with RHD.
文摘目的研究风湿性心脏病合并慢性心房颤动心房肌中FHL2蛋白水平的变化,探讨FHL2改变在慢性心房颤动中的作用。方法按性别、年龄、二尖瓣狭窄程度、左房大小及肺动脉压力配对,收集接受瓣膜置换手术的风湿性二尖瓣狭窄患者共28例(窦性组14例,房颤组14例)。在建立体外循环时采取右心耳组织及部分慢性心房颤动患者左心耳组织(7例),用Western blot法检测FHL2蛋白在心房肌中的表达水平。结果在右心耳水平,房颤组FHL2蛋白(0.68±0.28 vs 0.51±0.18,t=2.488,P=0.027)表达水平较窦性组明显上调。房颤组FHL2蛋白的表达量在右心耳与左心耳差异无统计学意义(0.58±0.22 vs 0.50±0.13,t=0.984,P=0.363)。结论 FHL2蛋白在风湿性心脏病合并慢性心房颤动心房肌中表达上调,其可能参与慢性心房颤动的发生及维持。而FHL2蛋白在慢性心房颤动左、右心房肌的表达无明显差异,表明右心耳标本替代左心耳在本研究中的结果是可信的。
