Objective:To study the effect of Helicobacter pylori infection on rheumatoid arthritis and T-lymphocyte subpopulations in patients with rheumatoid arthritis and to provide a new method for the treatment of rheumatoid ...Objective:To study the effect of Helicobacter pylori infection on rheumatoid arthritis and T-lymphocyte subpopulations in patients with rheumatoid arthritis and to provide a new method for the treatment of rheumatoid arthritis by removing Helicobacter pylori from patients.Methods:60 patients with rheumatoid arthritis admitted to the hospital from May 2022 to May 2023 were selected for the study,and all patients underwent a 13-carbon urea breath test to detect gastric H.pylori and the test results showed that 20 cases were negative and 40 cases were positive.The 40 positive patients were divided into the treatment group(n=20)and non-treatment group(n=20)by random number table method and the treatment group was given anti-Helicobacter pylori treatment,and the non-treatment group was given maintenance rheumatoid basic treatment,comparing the anti-cyclic citrulline peptide(CCP),DS28 score,peripheral blood T-lymphocyte subsets(CD4^(+)T-lymphocytes,CD8^(+)T-lymphocytes,CD4^(+)/CD8^(+)ratio)before and after the treatment of patients by 13-carbon urea respiration test(pylori-negative group,20 patients)and those who were positive for the treatment of H pylori(pylori-positive group,40 patients).Besides,the correlation of peripheral blood T-lymphocyte subsets and disease activity between treatment and non-treatment groups in the pylori-positive group was identified together with the correlation of DS28 scores,TNF-αlevels,sedimentation and immunoglobulin,lymphocyte subsets in the pylori-positive treatment group and positive non-treatment group as well as the level of globulin,lymphocyte subsets,and peripheral blood lymphocytes before and after treatment.Results:Before treatment,CCP,DS28 score,CD8^(+)T lymphocyte level of the pylori-negative group were lower than that of the positive group,and CD4^(+)T lymphocyte and CD4^(+)/CD8^(+)ratio were higher than that of the positive group(P<0.05);after treatment,the indexes of the pylori-positive group improved,and there was no significant difference in the comparison of the indexes with those of the pylori-negative group(P>0.05);the positive treatment group had a DS28(3.19±1.02)points,positive non-treatment group DS28(5.36±1.85)points,non-treatment group DS28 score and CD4^(+)T lymphocytes,CD4^(+)/CD8^(+)negative correlation with CD8^(+)T lymphocytes showed a positive correlation(P<0.05);before the treatment,pylori-positive treatment group and non-treatment group DS28 scores,TNF-αlevels,peripheral blood T lymphocyte subpopulation levels were not significantly different(P>0.05);after treatment,DS28 score,TNF-αlevel,CD8^(+)T of the treatment group were lower than those of the non-treatment group,and CD4^(+)T lymphocytes and CD4^(+)/CD8^(+)ratio were higher than those of the non-treatment group(P<0.05).Conclusion:H.pylori affects the level of T lymphocyte subsets in patients with rheumatoid arthritis,and there is a certain correlation between the two.Removal of H.pylori can improve the level of T lymphocyte subsets,which is important for the treatment of patients with rheumatoid arthritis.展开更多
Materials and Methods: lymphocytes of 10 pa-tients having early rheumatoid arthritis (RA) (the duration of the illness was 3 - 6 months) with a marked exudational process in joints were ex-amined. The content of lymph...Materials and Methods: lymphocytes of 10 pa-tients having early rheumatoid arthritis (RA) (the duration of the illness was 3 - 6 months) with a marked exudational process in joints were ex-amined. The content of lymphocytes expressing the CD3, CD4, CD8, CD16, CD56, CD20, CD72, CD38, CD23, CD25, CD71, HLA-DR, CD95, CD30, CD54, mIgM, mIgG antigens was determined. Results: the “Taban-Arshan” extract corrects the changes of the immune system characterized by the evident activation of the B-cell part of the immune system and normalizes immune parameters of the lymphocytes taken from the patients with autoimmune diseases (early rheumatoid arthritis). The immunocorrective effect of the “Taban-Arshan” extract is related to its ability to suppress the lymphocyte increased activation by normalizing expression of the main activation antigens (CD23, CD25, CD71, HLA-DR, CD54).展开更多
目的:观察类风湿关节炎(rheumatoid arthritis,RA)患者血清Ⅱ型胶原C端肽(typeⅡcollagen C-terminal peptide,CTX-Ⅱ)、组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)水平变化,分析其与免疫功能、疾病活动度的关系。方法:选取本院...目的:观察类风湿关节炎(rheumatoid arthritis,RA)患者血清Ⅱ型胶原C端肽(typeⅡcollagen C-terminal peptide,CTX-Ⅱ)、组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)水平变化,分析其与免疫功能、疾病活动度的关系。方法:选取本院收治的84例RA患者(RA组)和84例体检健康者(NC组)。分析血清CTX-Ⅱ、HDAC3(酶联免疫吸附法)及免疫功能指标[免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM、CD3^(+)、CD4^(+)、CD8^(+)T细胞比例]、疾病活动度相关指标[C反应蛋白(C-reactive protein,CRP)、血沉(erythrocyte sedimentation rate,ESR)]水平变化情况。Pearson法分析血清CTX-Ⅱ、HDAC3分别与免疫指标、疾病活动度相关指标的相关性。结果:RA组CTX-Ⅱ(9.26±1.84 vs 4.59±0.62)、HDAC3(83.72±13.51 vs 20.15±4.90)水平高于NC组(t=22.044、40.542,P<0.05)。缓解组CTX-Ⅱ(7.37±1.71 vs 10.75±1.94)、HDAC3(72.97±12.10 vs 92.18±14.62)水平低于活动组(t=8.347、7.940,P<0.05)。RA组CD3^(+)(64.42±6.91 vs 70.42±7.34)、CD4^(+)(34.80±5.96 vs 39.74±5.80)T细胞比例低于NC组,而CD8^(+)T细胞(31.68±6.15 vs 29.62±7.15)、IgM(2.06±0.39 vs 1.47±0.30)、IgA(2.96±0.50 vs 1.51±0.36)、IgG(15.49±3.05 vs 11.83±2.19)、ESR(28.32±7.60 vs 13.75±2.18)、CRP(10.94±2.02 vs 6.08±0.73)水平显著高于NC组(P<0.05)。活动组患者CD3^(+)(62.94±6.60 vs 66.30±7.30)、CD4^(+)T(33.57±5.74 vs 36.36±6.24)细胞低于缓解组,IgM(2.19±0.41 vs 1.89±0.36)、IgA(3.08±0.54 vs 2.81±0.45)、IgG(16.16±3.27 vs 14.64±2.77)、ESR(30.06±8.15 vs 26.11±6.90)、CRP(12.37±2.24 vs 9.12±1.74)水平高于缓解组(P<0.05)。相关性分析显示,血清CTX-Ⅱ与ESR、CRP、28个关节疾病活动(disease activity score in 28 joints,DAS28)呈正相关(r=0.572、0.495、0.809,P均=0.000);血清HDAC3分别与CD3^(+)、CD4^(+)T细胞比例存在负相关(r=-0.529、-0.464,P均=0.000),而与IgM、IgA、IgG、ESR、CRP、DAS28评分呈正相关(r=0.460、0.491、0.445、0.540、0.519、0.864,P均<0.05)。结论:类风湿关节炎患者血清CTX-Ⅱ、HDAC3水平均高于体检健康者,CTX-Ⅱ与RA疾病活动度有关,HDAC3与RA免疫功能紊乱以及疾病活动度有关。展开更多
文摘Objective:To study the effect of Helicobacter pylori infection on rheumatoid arthritis and T-lymphocyte subpopulations in patients with rheumatoid arthritis and to provide a new method for the treatment of rheumatoid arthritis by removing Helicobacter pylori from patients.Methods:60 patients with rheumatoid arthritis admitted to the hospital from May 2022 to May 2023 were selected for the study,and all patients underwent a 13-carbon urea breath test to detect gastric H.pylori and the test results showed that 20 cases were negative and 40 cases were positive.The 40 positive patients were divided into the treatment group(n=20)and non-treatment group(n=20)by random number table method and the treatment group was given anti-Helicobacter pylori treatment,and the non-treatment group was given maintenance rheumatoid basic treatment,comparing the anti-cyclic citrulline peptide(CCP),DS28 score,peripheral blood T-lymphocyte subsets(CD4^(+)T-lymphocytes,CD8^(+)T-lymphocytes,CD4^(+)/CD8^(+)ratio)before and after the treatment of patients by 13-carbon urea respiration test(pylori-negative group,20 patients)and those who were positive for the treatment of H pylori(pylori-positive group,40 patients).Besides,the correlation of peripheral blood T-lymphocyte subsets and disease activity between treatment and non-treatment groups in the pylori-positive group was identified together with the correlation of DS28 scores,TNF-αlevels,sedimentation and immunoglobulin,lymphocyte subsets in the pylori-positive treatment group and positive non-treatment group as well as the level of globulin,lymphocyte subsets,and peripheral blood lymphocytes before and after treatment.Results:Before treatment,CCP,DS28 score,CD8^(+)T lymphocyte level of the pylori-negative group were lower than that of the positive group,and CD4^(+)T lymphocyte and CD4^(+)/CD8^(+)ratio were higher than that of the positive group(P<0.05);after treatment,the indexes of the pylori-positive group improved,and there was no significant difference in the comparison of the indexes with those of the pylori-negative group(P>0.05);the positive treatment group had a DS28(3.19±1.02)points,positive non-treatment group DS28(5.36±1.85)points,non-treatment group DS28 score and CD4^(+)T lymphocytes,CD4^(+)/CD8^(+)negative correlation with CD8^(+)T lymphocytes showed a positive correlation(P<0.05);before the treatment,pylori-positive treatment group and non-treatment group DS28 scores,TNF-αlevels,peripheral blood T lymphocyte subpopulation levels were not significantly different(P>0.05);after treatment,DS28 score,TNF-αlevel,CD8^(+)T of the treatment group were lower than those of the non-treatment group,and CD4^(+)T lymphocytes and CD4^(+)/CD8^(+)ratio were higher than those of the non-treatment group(P<0.05).Conclusion:H.pylori affects the level of T lymphocyte subsets in patients with rheumatoid arthritis,and there is a certain correlation between the two.Removal of H.pylori can improve the level of T lymphocyte subsets,which is important for the treatment of patients with rheumatoid arthritis.
文摘Materials and Methods: lymphocytes of 10 pa-tients having early rheumatoid arthritis (RA) (the duration of the illness was 3 - 6 months) with a marked exudational process in joints were ex-amined. The content of lymphocytes expressing the CD3, CD4, CD8, CD16, CD56, CD20, CD72, CD38, CD23, CD25, CD71, HLA-DR, CD95, CD30, CD54, mIgM, mIgG antigens was determined. Results: the “Taban-Arshan” extract corrects the changes of the immune system characterized by the evident activation of the B-cell part of the immune system and normalizes immune parameters of the lymphocytes taken from the patients with autoimmune diseases (early rheumatoid arthritis). The immunocorrective effect of the “Taban-Arshan” extract is related to its ability to suppress the lymphocyte increased activation by normalizing expression of the main activation antigens (CD23, CD25, CD71, HLA-DR, CD54).
文摘目的:观察类风湿关节炎(rheumatoid arthritis,RA)患者血清Ⅱ型胶原C端肽(typeⅡcollagen C-terminal peptide,CTX-Ⅱ)、组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)水平变化,分析其与免疫功能、疾病活动度的关系。方法:选取本院收治的84例RA患者(RA组)和84例体检健康者(NC组)。分析血清CTX-Ⅱ、HDAC3(酶联免疫吸附法)及免疫功能指标[免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM、CD3^(+)、CD4^(+)、CD8^(+)T细胞比例]、疾病活动度相关指标[C反应蛋白(C-reactive protein,CRP)、血沉(erythrocyte sedimentation rate,ESR)]水平变化情况。Pearson法分析血清CTX-Ⅱ、HDAC3分别与免疫指标、疾病活动度相关指标的相关性。结果:RA组CTX-Ⅱ(9.26±1.84 vs 4.59±0.62)、HDAC3(83.72±13.51 vs 20.15±4.90)水平高于NC组(t=22.044、40.542,P<0.05)。缓解组CTX-Ⅱ(7.37±1.71 vs 10.75±1.94)、HDAC3(72.97±12.10 vs 92.18±14.62)水平低于活动组(t=8.347、7.940,P<0.05)。RA组CD3^(+)(64.42±6.91 vs 70.42±7.34)、CD4^(+)(34.80±5.96 vs 39.74±5.80)T细胞比例低于NC组,而CD8^(+)T细胞(31.68±6.15 vs 29.62±7.15)、IgM(2.06±0.39 vs 1.47±0.30)、IgA(2.96±0.50 vs 1.51±0.36)、IgG(15.49±3.05 vs 11.83±2.19)、ESR(28.32±7.60 vs 13.75±2.18)、CRP(10.94±2.02 vs 6.08±0.73)水平显著高于NC组(P<0.05)。活动组患者CD3^(+)(62.94±6.60 vs 66.30±7.30)、CD4^(+)T(33.57±5.74 vs 36.36±6.24)细胞低于缓解组,IgM(2.19±0.41 vs 1.89±0.36)、IgA(3.08±0.54 vs 2.81±0.45)、IgG(16.16±3.27 vs 14.64±2.77)、ESR(30.06±8.15 vs 26.11±6.90)、CRP(12.37±2.24 vs 9.12±1.74)水平高于缓解组(P<0.05)。相关性分析显示,血清CTX-Ⅱ与ESR、CRP、28个关节疾病活动(disease activity score in 28 joints,DAS28)呈正相关(r=0.572、0.495、0.809,P均=0.000);血清HDAC3分别与CD3^(+)、CD4^(+)T细胞比例存在负相关(r=-0.529、-0.464,P均=0.000),而与IgM、IgA、IgG、ESR、CRP、DAS28评分呈正相关(r=0.460、0.491、0.445、0.540、0.519、0.864,P均<0.05)。结论:类风湿关节炎患者血清CTX-Ⅱ、HDAC3水平均高于体检健康者,CTX-Ⅱ与RA疾病活动度有关,HDAC3与RA免疫功能紊乱以及疾病活动度有关。
