期刊文献+
共找到8,252篇文章
< 1 2 250 >
每页显示 20 50 100
Biological Characteristics of Trehalose and Its Protective Effect on Food Fermentation Starters during Lyophilization
1
作者 Yuhong YANG Yangdi ZHANG 《Agricultural Biotechnology》 CAS 2023年第4期45-48,共4页
This paper reviewed the unique biological function of trehalose and its mechanism of stabilizing biological macromolecules and the research progress in the protective effect of trehalose on lactic acid bacteria fermen... This paper reviewed the unique biological function of trehalose and its mechanism of stabilizing biological macromolecules and the research progress in the protective effect of trehalose on lactic acid bacteria fermentation starters during lyophilization in food production.The application of trehalose in food industry was prospected. 展开更多
关键词 TREHALOSE biological characteristics LYOPHILIZATION
下载PDF
Comparative Study on Biological and Commercial Characteristics of Multiple Varieties of Broccoli(Brassica oleracea L.var.italica Plenck)and Vegetable Soybean(Glycine max(L.)Merr.)
2
作者 Peng WANG Shengqun JIAO +5 位作者 Hongjie TANG Yanxue DANG Qingfu DU Jihui LI Bushuai ZHONG Bingguo SONG 《Agricultural Biotechnology》 CAS 2023年第2期28-31,37,共5页
[Objectives]This study was conducted to select vegetable soybean varieties(Glycine max(L.)Merr.)that are suitable for local cultivation and meet export requirements.[Methods]Through continuous years of comparative exp... [Objectives]This study was conducted to select vegetable soybean varieties(Glycine max(L.)Merr.)that are suitable for local cultivation and meet export requirements.[Methods]Through continuous years of comparative experiments on broccoli and vegetable soybean varieties,detailed biological characteristic and economic quality data of multiple varieties were obtained.[Results]Vegetable soybean variety Taiwan 75-3 had very prominent early-maturing trait,the highest quality(qualification rate),and higher yield than the control check(CK);and the early-maturing trait of vegetable soybean variety Kaohsiung 9 was also prominent,and its yield was higher than that of the CK.They could be promoted as the main vegetable soybean varieties for spring open field cultivation in this region.Among the tested broccoli varieties,Lake had a higher yield,and was relatively tolerant to cold.It had an early harvest period,and was planted as an early autumn variety in this region.Naihan Youxiu showed the highest yield,good quality,cold resistance,and strong adaptability,making it suitable for planting as a late autumn variety in this region.[Conclusions]This study provides technical guidance for the cultivation of local broccoli and vegetable soybean. 展开更多
关键词 Vegetable soybeans BROCCOLI biological characteristic Commercial character
下载PDF
Biological scaffold as potential platforms for stem cells:Current development and applications in wound healing
3
作者 Jie-Yu Xiang Lin Kang +7 位作者 Zi-Ming Li Song-Lu Tseng Li-Quan Wang Tian-Hao Li Zhu-Jun Li Jiu-Zuo Huang Nan-Ze Yu Xiao Long 《World Journal of Stem Cells》 SCIE 2024年第4期334-352,共19页
Wound repair is a complex challenge for both clinical practitioners and researchers.Conventional approaches for wound repair have several limitations.Stem cell-based therapy has emerged as a novel strategy to address ... Wound repair is a complex challenge for both clinical practitioners and researchers.Conventional approaches for wound repair have several limitations.Stem cell-based therapy has emerged as a novel strategy to address this issue,exhibiting significant potential for enhancing wound healing rates,improving wound quality,and promoting skin regeneration.However,the use of stem cells in skin regeneration presents several challenges.Recently,stem cells and biomaterials have been identified as crucial components of the wound-healing process.Combination therapy involving the development of biocompatible scaffolds,accompanying cells,multiple biological factors,and structures resembling the natural extracellular matrix(ECM)has gained considerable attention.Biological scaffolds encompass a range of biomaterials that serve as platforms for seeding stem cells,providing them with an environment conducive to growth,similar to that of the ECM.These scaffolds facilitate the delivery and application of stem cells for tissue regeneration and wound healing.This article provides a comprehensive review of the current developments and applications of biological scaffolds for stem cells in wound healing,emphasizing their capacity to facilitate stem cell adhesion,proliferation,differentiation,and paracrine functions.Additionally,we identify the pivotal characteristics of the scaffolds that contribute to enhanced cellular activity. 展开更多
关键词 Stem-cell-based therapy biological scaffolds Wound healing Extracellular matrix mimicry Cellular activities enhancement Scaffold characteristics
下载PDF
Study on Pathogeny Identification and Biological Characteristics of Anthracnose in Cassava 被引量:6
4
作者 蔡吉苗 李超萍 +2 位作者 时涛 林春花 黄贵修 《Agricultural Science & Technology》 CAS 2010年第3期129-134,共6页
[Objective] The research aimed to isolate and identify the anthracnose pathogen of Cassava and study its biological charcteristics.[Method] Two isolates of anthracnose (CCGHN01 and CCGHN03) in Cassava were isolated ... [Objective] The research aimed to isolate and identify the anthracnose pathogen of Cassava and study its biological charcteristics.[Method] Two isolates of anthracnose (CCGHN01 and CCGHN03) in Cassava were isolated from the diseased leaves collected from Hainan of China.They were identified through the morphological observation of conidia and ITS sequence analysis.And the morphological characteristics were studied.[Result] The morphological observation of conidia and ITS sequence analysis indicated that the two isolates were Colletotrichum gloeosporioides.The morphological characteristics study results showed that optimum medium for the growth of two strains was PSA,the optimum temperatures were 26 ℃ and 30 ℃ respectively,the optimum pH was 8.0,the optimum light conditions were alternative light and dark and complete darkness respectively.For the conidia germination of two strains,the optimum temperatures were 28 ℃ and 30 ℃ respectively and the lethal temperature was 55 ℃ remaining 10 min.[Conclusion] The research laid the foundation for further control of anthracnose in Cassava. 展开更多
关键词 CASSAVA ANTHRACNOSE biological characteristics
下载PDF
Biological Characteristics and Pathogenicities of Shewanella algae and Shewanella abalone from Babylonia 被引量:2
5
作者 李淑芳 张继东 +2 位作者 邱德全 杨世平 黄子通 《Agricultural Science & Technology》 CAS 2015年第9期1845-1850,1859,共7页
[Objective] The biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia were studied in this paper. [Method]The hemolytic bacteria were isolated from the hepatopancreas... [Objective] The biological characteristics and pathogenicities of Shewanella algae and Shewanella abalone from Babylonia were studied in this paper. [Method]The hemolytic bacteria were isolated from the hepatopancreas of Babylonia suffered from proboscis edema with blood agar plate. The dominant bacterial community in the ill Babylonia was identified by 16 S r DNA sequence analysis, and the bacterial cultural and biochemical characteristics and pathogenicities were studied. [Result]The Shewanella bacteria, including Shewanella algae and Shewanella abalone, are the dominant bacterial community in Babylonia suffered from proboscis edema.The colony characteristics of Shewanella algae in nutrient agar medium, TCBS agar medium and CHROMagar vibrio colored medium were similar to those of Shewanella abalone. Shewanella algae possessed β-hemolysis and Shewanella abalone possessed α-hemolysis in the blood agar plate. The biochemical reaction of Shewanella algae and Shewanella abalone was all of non-fermentation type. The results of artificial infection test showed that half lethal dose(LD50) of the test strains of Shewanella algae was 10-5.50/0.1 ml. The test strains of Shewanella algae have strong toxicity, and could cause mice and chickens to die of sepsis with mortality of100%. The mortality of Babylonia infected with Shewanella algae was 10%; while the survived Babylonia lost the ability of moving and intaking for a long time, but they were not suffered from proboscis edema. There was no death in mice or chicks infected with Shewanella abalone, but their livers and spleens were slightly hyperemic and swelling. There was also no death in Babylonia infected with Shewanella abalone, but their intaking and moving ability was lost for a short time.[Conclusion] Although Shewanella algae and Shewanella abalone were the dominant bacteria in Babylonia suffered from proboscis edema, they were not the main pathogenic bacteria for proboscis edema. Shewanella algae had strong pathogenicity to mice, chicks and Babylonia, while Shewanella abalone showed no marked pathogenicity to those experimental animals in this study. 展开更多
关键词 Shewanella algae Shewanella abalone biological characteristics PATHOGENICITY Babylonia
下载PDF
Isolation and Identification of Colletotrichum gloeosporioides in Pears and Its Biological Characteristics 被引量:1
6
作者 刘邮洲 陈志谊 +3 位作者 钱国良 聂亚峰 乔俊卿 刘凤权 《Agricultural Science & Technology》 CAS 2014年第2期191-195,共5页
[Objective] This study aimed to explore the biological characteristics of Col etotrichum gloeosporioides in pears. [Method] Twenty-five C. gloeosporioides strains were isolated and identified from the diseased samples... [Objective] This study aimed to explore the biological characteristics of Col etotrichum gloeosporioides in pears. [Method] Twenty-five C. gloeosporioides strains were isolated and identified from the diseased samples. Their pathogenicity was identified by inoculating the surface of punctured pears with fungal discs. The effects of different temperatures, pH values, carbon sources and nitrogen sources on the growth of C. gloeosporioides mycelia were explored by incubating fungal discs on the center of plates. [Result] Among the twenty-five C. gloeosporioides strains, three had strong pathogenicity, and eighteen had intermediate pathogenicity, and four strains had weak pathogenicity. Those highly-pathogenic strains had darker colonies, with dense mycelia, whereas those lowly-pathogenic ones had white colonies, with sparse mycelia. Those with fast-growing colonies showed strong pathogenicity, while those with slowly-growing colonies displayed weak pathogenicity. There was no relationship between conidia yield and pathogenicity. The optimum temperature for the growth of C. gloeosporioides mycelia was 25-30 ℃, and the optimum pH was 5.0-7.0. C. gloeosporioides could make use of various carbon sources (monosaccharide and disaccharide), inorganic and organic nitrogen sources, and the optimal carbon source and nitrogen source were sucrose and beef extract, respectively. [Conclusion] Our study benefits further understanding of C. gloeospori-oides and helps to control pear anthracnose more effectively. 展开更多
关键词 Colletotrichum gloeosporioides PATHOGENICITY biological characteristics
下载PDF
Identification and Biological Characteristics of Blank Spot Pathogen from Lilium lancifolium 被引量:3
7
作者 吴金平 丁自立 +3 位作者 刘晓艳 郭凤领 焦忠久 邱正明 《Agricultural Science & Technology》 CAS 2014年第11期1879-1882,共4页
With the increasing planting area of Lilium lancifolium, the leaf fungal dis-ease of L. lancifolium is becoming more and more serious. In June and July of 2014, the excessive rainfal leads to the serious outbreak of l... With the increasing planting area of Lilium lancifolium, the leaf fungal dis-ease of L. lancifolium is becoming more and more serious. In June and July of 2014, the excessive rainfal leads to the serious outbreak of leaf disease of L. lanci-folium. In mid-June, the wilting rate of L. lancifolium in seriously-infected field was even up to 50%-70%. In some fields, the shoots of L. lancifolium even al wilted. The pathogen was isolated from the infected leaves of L. lancifolium. Its pathogenici-ty, spore morphology, 18S rDNA sequence and biological characteristics were stud-ied. The results showed the isolated pathogen was Alternaria alternate. The lethal temperature of mycelial growth was 55 ℃. The optimum pH was 6-7. Among the tested carbon sources and nitrogen sources, the optimum carbon source was mal-tose, and the optimum nitrogen source was yeast extract. 展开更多
关键词 Lilium lancifolium Black spot biological characteristic Molecular identifi-cation
下载PDF
Observation of Biological Characteristics of Bananamoth[Opogona sacchari (Bojer)]Larvae in Sugarcane
8
作者 魏吉利 黄诚华 +3 位作者 潘雪红 商显坤 王伯辉 黄冬发 《Plant Diseases and Pests》 CAS 2011年第2期25-27,共3页
[ Objective] The paper was to understand the biological characteristics of bananamoth [ Opogona sacchari ( Bojer ) ] larvae in sugarcane. [ Method ] Through the rearing test of bananamoth larvae with different hosts... [ Objective] The paper was to understand the biological characteristics of bananamoth [ Opogona sacchari ( Bojer ) ] larvae in sugarcane. [ Method ] Through the rearing test of bananamoth larvae with different hosts, the biological characteristics of bananamoth larvae in sugarcane were observed and analyzed. [ Result ] The mortality rate of bananamoth larvae reared with sugarcane was higher than that of the pests reared with potato. The larva had 8 instars, and the devel- opment duration of the larvae reared with sugarcane was 34.6 d longer than the pests reared with potato. The larvae needed about 98.8 d to complete a generation in average. Viewed from the observation values of head width, body length and weight of larvae, bananamoth larvae reared with sugarcane basically accorded with the growth and development law of larvae. [Condusion ] The results provided theoretical basis for the preparation of effective measures against bananamoth and the assurance of safe production of.sugarcane. 展开更多
关键词 Bananamoth SUGARCANE biological characteristics Larva
下载PDF
Biological Characteristics and Pathogenicity of Verticillium lecanii Isolated from Naturally Died Boettcherisca peregrine
9
作者 王凌超 高熹 +3 位作者 王松田 刘小文 叶敏 吴国星 《Plant Diseases and Pests》 CAS 2010年第2期28-31,40,共5页
[Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and... [Objective] In order to find the pathogenic microorganisms suitable for biological control of filth flies, the pathogenic microorganism was isolated from the dead fly, Boettcherisca peregrine. [Method] The conidia and mycelia were observed by optical microscope. The pathogenic microorganism was identified on the basis of its culture characters and the optical morphologies of the conidia and mycelia, and its biological characteristics and pathogenicity were preliminarily studied. [Result] The pathogenic microorganism isolated from the dead fly was a new strain of Verticillium lecanii. The new strain of V. lecanii was numbered as KMZW-1. The colonies of V. lecanii KMZW-1 grew fastest on potato dextrose agar medium (PDA) at 29 ℃ and pH 6.0. The LC50 of its spore suspension to the adults of B. peregrine, Lucilia sericata, Musca domestic, Piophila casei and Drosophila melanogaster were 9.50×10^5, 4.58×10^7, 4.06×10^7, 4.10×10^3 and 1.05×10^7 conidia/ml, respectively. The LT50 were 6.86, 8.17, 8.16, 8.12 and 3.22 d, respectively. [Conclusion] V. lecanii KMZW-1 is an active pathogenic microorganism to control the adults of five fly species. 展开更多
关键词 Boettcherisca peregrine Verticillium lecanii biological characteristic PATHOGENICITY
下载PDF
Identification and Biological Characteristics of Pineapple Anthracnose in Hainan Province
10
作者 罗志文 范鸿雁 +1 位作者 何凡 华敏 《Plant Diseases and Pests》 CAS 2010年第5期21-25,共5页
[Objective]The pathogen of pineapple anthracnose were isolated and identified,and the biological characteristics of the pathogen were studied.[Method]Pathogen was isolated and purified from the leaves collected from d... [Objective]The pathogen of pineapple anthracnose were isolated and identified,and the biological characteristics of the pathogen were studied.[Method]Pathogen was isolated and purified from the leaves collected from different areas in Hainan province which had been infected by pineapple anthracnose,and the biological characteristics were studied as well as morphological identification of the pathogen.[Results] The pathogen of pineapple anthracnose was identified as Colletorichum gloeosprioides Penz.through morphological characteristics.The results of biological characteristic test showed that the optimum temperature for mycelium growth and spore germination were 25-30 and 28-30 ℃,respectively;and the optimum pH value was 6.0-8.0;complete darkness was the best condition for growth of mycelium,while alternative light and darkness was most beneficial for spore germination;the best carbon sources for mycelium growth were glucose,D-fructose and maltose;except for L-arabinose and D-galactose,the other carbon sources all had promotion effect on spore germination;yeast extract,peptone and beef extract were proved to be beneficial for mycelium growth and spore germination.Aspartic acid was only beneficial for spore germination;spores were just able to germinate when the relative humidity(RH)was greater than 90%,spore germination was the highest under water drops condition.[Conclusion]The results laid preliminary basis for the control of pineapple anthracnose. 展开更多
关键词 PINEAPPLE Colletorichum gloeosprioides Penz. biological characteristics
下载PDF
Biological Characteristics of Biosurfactantproducing Petroleum-degrader Bacterium Bacillus BS-8
11
作者 常慧萍 邢文会 +3 位作者 夏铁骑 付瑞敏 陶令霞 张红 《Agricultural Science & Technology》 CAS 2016年第1期1-3,18,共4页
The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, s... The growth characteristics of petroleum-degrader BS-8(Bacillus sp.) and the factors influencing its biosurfactant production were tested; the biosurfactant releasing mode of BS-8 was speculated by measuring OD600, surface tension and oil displacement of fermentation broth; and the effects of carbon source, nitrogen source, temperature, p H, and Na Cl concentration on biosurfactant production by BS-8 were observed in this study. The results showed that the biosurfactant releasing mode of BS-8 was growth-correlated, the surface tension of BS-8 fermentation broth declined with the total bacteria increasing, and the oil displacement was positive correlated with biosurfactant content in fermentation broth; and the optimal culture conditions for effective biosurfactant production included: glucose as carbon source,yeast extract as nitrogen source, Na Cl concentration of 2%, p H of 7.0 and temperature at 30 ℃. 展开更多
关键词 BIOSURFACTANT Petroleum-degrader BS-8 Growth-correlated biological characteristic
下载PDF
Biological Characteristics of Ginseng Botrytis cinerea Pers
12
作者 李熙英 李烨 《Plant Diseases and Pests》 CAS 2010年第5期10-14,共5页
[ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tiss... [ Objective ] The study systematically studied the biological characteristics of ginseng Botrytis cinerea Pers. [ Method ] The pure pathogenic fungus was isolated from ginseng B. cinerea collected in the field by tissue segregation and purification cultivation. Subsequently, using PDA medium plate culture method, the effect of various culture conditions on mycelium growth and sporulation of ginseng B. cinerea was detected. [ Result] The optimum temperature for mycelium growth and sporulation of B. cinerea was 25 ℃. The appropriate temperature for conidia germination ranged from 20 to 25 ℃. And the optimum pH value for mycelium growth and conidia germination was 6.0. The optimum carbon source was sucrose, followed by glucose and fructose. The optimum nitrogen source was peptone, fol- lowed by beef extract, yeast extract, alanine and ammonium nitrate. Among the media, the growth of mycelium cultured on PDA medium was the fastest with the production of gray mycelium and dense colonies. Lethal temperatures for sclerotia, mycelium and conidia were 60, 55 and 50 ℃, respectively. [ Conclusion] The study provided the scientific basis for the research on the incidence law of B. cinerea and its control. 展开更多
关键词 Botrytis cinerea Pers MYCELIUM CONIDIA biological characteristics
下载PDF
Relationship between Biological Characteristics of Beauveria bassiana (Bals.) Vuill and Pathogenicity to Bombyx mori L.
13
作者 骆海玉 邓业成 +1 位作者 廖永梅 李瑞钰 《Agricultural Science & Technology》 CAS 2012年第9期1919-1923,共5页
[Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the... [Objective] This study was to investigate the relationship between biological characteristics of Beauveria bassiana (Bals.) Vuill and pathogenicity to Bombyx rnori L, with the aim to provide scientific basis for the control of white muscardine in Bombyx mori L. [Method] The strains isolated and purified from the 6 Beauveria bassiana biocontrol agents from all over the country and the 3 white muscardine silkworms collected from Guangxi provincial silkworm rearing areas were identified by the morphological observation and molecular biology technology. The pathogenicity of B. bassaina to silkworms was determined, and the biological characteristics such as growth diameter, sporulation and the extracellular protease activity of the different B. bassiana strains were compared. [Result] The isolated 9 strains were all B. bassaina (Bals.) Vuillemin, and all strains had high pathogenicity to silkworm, but with different pathogenicities. The growth diameter, sporulation and extracellular protease activity of different B. bassiana strains were also different, and showed correlation with the patheogenicity to silkworms. [Conclusion] B. bassiana spores production amount and exocellular protease activity had significant positive correlation with their pathogenicity to silkworm. 展开更多
关键词 Beauveria bassiana (Bals.) Vuill biological characteristics PATHOGENICITY Bombyx mori L.
下载PDF
Study on the biological characteristics of Homalotglus flaminus
14
作者 马玲 林同 《Journal of Forestry Research》 SCIE CAS CSCD 2001年第4期269-270,278,共3页
Homalotglus flaminus (Dalman) is an important natural enemy of the Chilocorus kuwanae. Its biological character-istic, life history, the action style on hosts were studied at Hongqi Forest Farm, Daqing City, Heilongji... Homalotglus flaminus (Dalman) is an important natural enemy of the Chilocorus kuwanae. Its biological character-istic, life history, the action style on hosts were studied at Hongqi Forest Farm, Daqing City, Heilongjiang Province from June 1998 to October 2000, in order to control H. flaminus and raise the control ability of Chilocorus kuwanae to Quadraspidiotus gigas pest. The observed results indicated that H. flaminus has two generation one year in study area, the pupating span is 12.7 d, life span of females and males is 17.6 d and 10.8 d respectively, egg span is 10 d, and the sex ratio of females to males is 1:3. 展开更多
关键词 Homalotglus flaminus Chilocorus kuwanae biological characteristics
下载PDF
Biological Characteristics of Skeletal Muscle Satellite Cells and Its Application Prospects in Muscle Trauma Repair 被引量:1
15
作者 元虹懿 袁子奥 +1 位作者 支晓亮 张明海 《Agricultural Science & Technology》 CAS 2016年第10期2349-2353,共5页
As the only source of stem cells, satellite cells play and extraordinary role in the remediation process of skeletal muscle after injury. This paper overviewed the biological characteristic of skeletal muscle satellit... As the only source of stem cells, satellite cells play and extraordinary role in the remediation process of skeletal muscle after injury. This paper overviewed the biological characteristic of skeletal muscle satellite cells and its role in repairing muscle injury, and put forward the prospects of its application in muscle trauma repair. 展开更多
关键词 Satellite cell Skeletal muscle biological characteristics REPAIR
下载PDF
Biological Characteristics of 11 Wilt Disease Pathogens on Hybrid Bamboo
16
作者 梁祖昂 蔡吉苗 +3 位作者 刘先宝 李超萍 时涛 黄贵修 《Plant Diseases and Pests》 CAS 2010年第4期4-8,共5页
[Objective] The biological characteristics of 11 wilt disease pathogens on hybrid bamboo were studied. [Method] 11 F. oxysporum strains of hybrid bamboo were isolated from diseased bamboo tissue in the bamboo base of ... [Objective] The biological characteristics of 11 wilt disease pathogens on hybrid bamboo were studied. [Method] 11 F. oxysporum strains of hybrid bamboo were isolated from diseased bamboo tissue in the bamboo base of Guangxi Huajing Bamboo Development Co., Ltd.. Biological characteristics of these pathogens were studied. [ Result] The optimum growth medium and light conditions for 11 strains were different, the optimum growth temperature was 24 -28 ℃, the optimum pH value was 7.0 - 11.0, lactose and D-sorbitol were the best carbon sources, and yeast extract was the best nitrogen source. The optimum temperature for oonidia germination was about 24 -30 ℃3, the best carbon source were maltose, lactose and sucrose, the best nitrogen source was yeast extract and beef extract. The lethal temperature of conidia was 50 ℃ (10 min). [ Cendusioal The study supplied good theoretical basis for the control of Fusarium wilt disease in hybdd bamboo. 展开更多
关键词 Hybrid bamboo Wilt disease biological characteristics
下载PDF
Invertebrate community characteristics in biologically active carbon filter 被引量:5
17
作者 Xiaowei Li Yufeng Yang +2 位作者 Lijun Liu Jinsong Zhang Qing Wang 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2010年第5期648-655,共8页
Biologically active carbon (BAC) system was set up in a water plant of South China during January to December 2007,to study the invertebrate community characteristics of BAC filter.Thirty-seven invertebrate species ... Biologically active carbon (BAC) system was set up in a water plant of South China during January to December 2007,to study the invertebrate community characteristics of BAC filter.Thirty-seven invertebrate species were found,of which 28 belonging to rotifers.Filter operation could lead to an output of invertebrates in high abundances with the filtrate,and the maximum density could reach 5608 individuals/m 3.Average abundances in the effluent water increased in 27-33 folds in comparison to the influent water during the sampling period.Invertebrate community succession had the following trend:filter-feeding animals → small benthic invertebrates → large benthic and resistant invertebrates.Abundances of large-sized invertebrates (copepod adult and oligochaete) at bigger-media column were significantly higher than that at small-media column.The results implied the abundant species diversity of invertebrate in BAC filter.The relationship between invertebrate and biofilm still remain to be studied in detail. 展开更多
关键词 biologically active carbon filter INVERTEBRATE community characteristics filter media
下载PDF
In vitro growth, differentiation and biological characteristics of neural stem cells 被引量:20
18
作者 Meijiang Yun Lianzhong Wang +1 位作者 Yongcai Wang Xiaolian Jiang 《Neural Regeneration Research》 SCIE CAS CSCD 2006年第4期364-367,共4页
OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was ... OBJECTIVE: To summarize the biological characteristics of neural stem cells, and the separation, purification. differentiation and source of neural stem cells. DATA SOURCES : An online search of Pubmed database was undertaken to identify English articles about the growth of neural stem cells in vitro published from January 2000 to October 2006 by using the keywords of "neural stem cells, bone marrow mesenchymal stem cells (BMSCs), umbilical cord blood stem cells, embryonic stem cells (ESC), separation methods, neural growth factor". And relevant articles published in IEEE/IEE Electronic Library (IEL) database, Springer Link database and Kluwer Online Journals were also searched, Chinese relevant articles published between January 2000 to October 2006 were searched with the same keywords in Chinese in Chinese journal full-text database. STUDY SELECTION : The articles were primarily screened, and then the full-texts were searched. Inclusive criteria: (1) Articles relevant to the biological characteristics and classification of neural stem cells; (2) Articles about the source, separation and differentiation of the ESCs, BMSCs and umbilical cord blood stem cells. The repetitive studies and reviews were excluded. DATA EXTRACTION : Thirty articles were selected from 203 relevant articles according to the inclusive criteria Articles were excluded because of repetition and reviews. DATA SYNTHESES : Neural stem cells have the ability of self-renewing and high differentiation, and they are obtained from ESCs, nerve tissue, nerve system, BMSCs and umbilical cord blood stem cells. ESCs can be separated by means of mechanical dissociation is better than that of the trypsin digestion, BMSCs by density gradient centrifuge separation, hemolysis, whole-blood culture, etc., and umbilical cord blood stem ceils by Ficoil density gradient centrifugation, hydroxyethyl starch (HES) centrifugation sedimentation, etc. Neural growth factor (NGF) and other factors play an important role in the growth of NSCs, such as transforming growth factor (TGF) is an important player in repairing organs, NGF accelerates the process of growth, insulin-like growth factor serves importantly in the differentiation of stem cells into neuron-like cells. CONCLUSION : As unipotent stem cells, NSCs have the abilities of self-renewal and potential of high differentiation. The method of mechanical dissociation is better than trypsin digestion in e separating ESCs. However, density gradient centrifuge separation is better than other methods in the separation of the BMSCs. NGF and other factors play an important role in the growth of NSCs. 展开更多
关键词 NSCS CELL STEM differentiation and biological characteristics of neural stem cells In vitro growth BMSCS
下载PDF
Construction of the tissue engineering seed cells(HaCaT-EGF) and analysis of its biological characteristics 被引量:2
19
作者 Ke Tao Xiao-Zhi Bai +5 位作者 Zhan-Feng Zhang Ji-Hong Shi Xiao-Qiang Li Chao-Wu Tang Da-Hai Hu Jun-Tao Han 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2013年第11期893-896,共4页
Objective:To construct the tissue engineering seed cell(HaCaT cell line) with stable expression of the human epidermal growth factor(EGF),and analyze the changes of its biological characteristics.Methods:PCDNA3.1-EGF ... Objective:To construct the tissue engineering seed cell(HaCaT cell line) with stable expression of the human epidermal growth factor(EGF),and analyze the changes of its biological characteristics.Methods:PCDNA3.1-EGF eukaryotic expression vector was transferred into HaCaT cell,and G418 was utilized to select the HaCaT-EGF cell line.Using an inverted microscope,PCR,ELISA method to detect the changes of the cell morphology,the expression of the ECF gene and protein,and the mRNA expression levels of apoptosis related molecule Caspase-3,the cell cycle related protein cyclin D1.Results:The mRNA expression levels of the obtained HaCaT-ECF cell were more than 100 times higher than the level of ordinary HaCaT cell.The colony of the HaCaT-EGF cells was more focused and tight compared to the empty vector transfected HaCaT cells and normal HaCaT cells.The expression levels of apoptotic factor Caspase-3 and cyclin Dt in HaCaT-EGF cell were significantly higher than those in the empty vector HaCaT- pcDNA3.1 cell,and the differences were statistically significant(P<0.01),but there was no significant difference compared to the normal HaCaT cells(P>0.05).Conclusions: HaCaT-EGF cell can continuously secrete ECF,and the biological characteristic is stable.It can be used for tissue engineering experiment and is an ideal seed cell for constructing tissue engineered skin. 展开更多
关键词 TISSUE engineering HACAT Gene TRANSFECTION biological characteristics
下载PDF
Biological characteristics of marine bacterium S-9801 strain and its culture conditions of pigment production 被引量:2
20
作者 Tian Li He Peiqing +3 位作者 Wu Hongqing Wen Zhanbo Liu Chenlin Li Guangyou 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2002年第4期541-545,共5页
Strain of Flavobacterium sp.(S-9801),was screened from 207 strains of marine bacteria isolated from the Bohai Sea continental shelf and the Zhujiang Estuary,for its red pigment production.The biological characteristic... Strain of Flavobacterium sp.(S-9801),was screened from 207 strains of marine bacteria isolated from the Bohai Sea continental shelf and the Zhujiang Estuary,for its red pigment production.The biological characteristics of strain S-9801 and culture conditions of pigment production have been checked out in this study. The color of the bacterial colony on 2216E medium was from coccineus to rose bengal. Optimum culture conditions were sodium chloride concentration(g/dm3),10~30;pH,3~8;temperature,25~28℃;tryptone and yeast extract as nitrogen sources and glucose as carbon source. Under optimum conditions,pigment accumulation started after 12 h,reaching a maximum rate of synthesis at 36 h. 展开更多
关键词 Marine bacteria pigment production biological characteristics culture conditions
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部