Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens ont...Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are not well understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing(TRV-HIGS) system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by infection with the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is a key pathogenic factor. Small RNA sequencing showed that the HIGS vectors were processed into si RNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be an excellent candidate for HIGS, thereby enhancing crop resistance against the pathogen and contributing to the control of R. solani infection.展开更多
Pear is a fruit crop of worldwide importance and cold storage is an integral part of the production and distribution of pears.An uncharacterized fungal disease has been observed on‘Huangguan’pear fruit during cold s...Pear is a fruit crop of worldwide importance and cold storage is an integral part of the production and distribution of pears.An uncharacterized fungal disease has been observed on‘Huangguan’pear fruit during cold storage in Hebei Province.The fungus was consistently isolated from diseased fruit by routine tissue separation method,and shown to be the causal agent according to Koch postulates.Based on its morphology,molecular characteristics,pathogenicity and ITS sequence,the fungus was identified as Rhizoctonia solani.This study recorded postharvest fruit rot caused by Rhizoctonia solani on pear fruit in China.展开更多
The necrotrophic fungus Rhizoctonia cerealis is the causal agent of devastating diseases of cereal crops including wheat(Triticum aestivum).We present a high-quality genome assembly of R.cerealis Rc207,a virulent stra...The necrotrophic fungus Rhizoctonia cerealis is the causal agent of devastating diseases of cereal crops including wheat(Triticum aestivum).We present a high-quality genome assembly of R.cerealis Rc207,a virulent strain causing wheat sharp eyespot.The assembly(56.36 Mb)is composed of 17.87%repeat sequences and 14,433 predicted protein-encoding genes.The Rc207 genome encodes a large and diverse set of genes involved in pathogenicity,especially rich in those encoding secreted proteins,carbohydrateactive enzymes(CAZymes),peptidases,nucleases,cytochrome P450,and secondary metabolismassociated enzymes.Most secretory protein-encoding genes,including CAZymes,peroxygenases,dehydrogenases,and cytochrome P450,were up-regulated during fungal infection of wheat.We identified 831 candidate secretory effectors and validated the functions of 10 up-regulated candidate effector proteins.Of them,nine were confirmed as necrotrophic pathogen’s effectors promoting fungal infection.Abundant potential mobile or plastic genomic regions rich in repeat sequences suggest their roles in fungal adaption and virulence-associated genomic evolution.This study provides valuable resources for further comparative and functional genomics on important fungal pathogens,and provides essential tools for development of effective disease control strategies.展开更多
[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by...[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by reverse transcription-polymerase chain reaction（RT-PCR）.Then RT-PCR products were cloned into the PMD18-T vector and sequenced.The functions of the sequence were predicted with bioinformatics method.[Result] A 1 079 bp gene was obtained.The gene encoded a protein with 236 amino acids.The protein contains many motif sites,two WRKY domains and a C2H2 zinc finger motif.The gene showed high identities with WRKY8,WRKY24 and WRKY30 gene of rice.[Conclusion] The up-regulated expression gene induced by R.solani was representative WRKY family gene.The gene could play an important role on rice sheath blight resistance.展开更多
本研究采用平板对峙法、菌丝生长速率法和菌核萌发抑制法筛选出一株细菌LB6-2,对立枯丝核菌的菌丝生长抑制率为71.76%,菌核萌发抑制率为100.00%。通过观察形态学特征、分析理化性质结合16S r DNA扩增序列分析对该菌株鉴定为萎缩芽胞杆菌...本研究采用平板对峙法、菌丝生长速率法和菌核萌发抑制法筛选出一株细菌LB6-2,对立枯丝核菌的菌丝生长抑制率为71.76%,菌核萌发抑制率为100.00%。通过观察形态学特征、分析理化性质结合16S r DNA扩增序列分析对该菌株鉴定为萎缩芽胞杆菌Bacillusatrophaeus(OK639005.1)。采用大孔树脂法提取发酵产物并进行HPLC制备,对获得的14个组分进行活性测定确定其活性组分,从活性组分中制备获得3个单品化合物LBN4-2、LBN4-4、LBN4-5,经HPLC-MS、NMR分析和活性测定结果表明,化合物LBN4-2为Cyclo(Pro-Val)、LBN4-4为Cyclo(D-Leu-L-Pro)和LBN4-5为Cyclo(L-prolyl-D-phenylalanyl),3个化合物的浓度在4 mmol/L时对立枯丝核菌菌丝生长的抑制率分别为86.16%、84.87%和83.10%,浓度在2 mmol/L以上时,对立枯丝核菌菌核萌发的抑制率为100%。由此可见,萎缩芽胞杆菌LB6-2具有作为防治立枯丝核菌生防菌剂研发的潜力。展开更多
分别基于7个烟草靶斑病菌株基因组中ITS-5.8S r DNA序列设计引物和探针;并对引物及探针特异性进行验证;建立检测体系并对接种烟草靶斑病菌的叶片和土壤中的烟草靶斑病菌进行检测。结果表明:所设计的引物及探针对R.solani AG-3具有特异性...分别基于7个烟草靶斑病菌株基因组中ITS-5.8S r DNA序列设计引物和探针;并对引物及探针特异性进行验证;建立检测体系并对接种烟草靶斑病菌的叶片和土壤中的烟草靶斑病菌进行检测。结果表明:所设计的引物及探针对R.solani AG-3具有特异性,检测体系可以检测出烟草叶片及土壤样品中的烟草靶斑病菌。接种烟草叶片的检测表明,接种后6h就可检测到强致病力菌株YC-9,12h后能检测到弱致病力菌株LF-2;获得了烟草靶斑病菌DNA质量的对数与添加菌丝量的对数之间的回归曲线方程,对不同月份土壤样品的测定结果表明烟草靶斑病菌在土壤中呈周年动态变化趋势。展开更多
基金supported by the Henan Province Science and Technology Research Project, China (Grant No. 242102110232)the National Natural Science Foundation of China (Grant No. 31801677)the Major Program of Guangdong Basic and Applied Basic Research, China (Grant No. 2019B030302006)。
文摘Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are not well understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing(TRV-HIGS) system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by infection with the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is a key pathogenic factor. Small RNA sequencing showed that the HIGS vectors were processed into si RNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be an excellent candidate for HIGS, thereby enhancing crop resistance against the pathogen and contributing to the control of R. solani infection.
基金supported by the National Key R&D Program of China(Grant No.2016YFD0400903–06)the earmarked fund for China Agriculture Research System(Grant No.CARS-29–19)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。
文摘Pear is a fruit crop of worldwide importance and cold storage is an integral part of the production and distribution of pears.An uncharacterized fungal disease has been observed on‘Huangguan’pear fruit during cold storage in Hebei Province.The fungus was consistently isolated from diseased fruit by routine tissue separation method,and shown to be the causal agent according to Koch postulates.Based on its morphology,molecular characteristics,pathogenicity and ITS sequence,the fungus was identified as Rhizoctonia solani.This study recorded postharvest fruit rot caused by Rhizoctonia solani on pear fruit in China.
基金funded by the National Key Project for Research on Transgenic Biology,China(2016ZX08002001 to Zengyan Zhang)。
文摘The necrotrophic fungus Rhizoctonia cerealis is the causal agent of devastating diseases of cereal crops including wheat(Triticum aestivum).We present a high-quality genome assembly of R.cerealis Rc207,a virulent strain causing wheat sharp eyespot.The assembly(56.36 Mb)is composed of 17.87%repeat sequences and 14,433 predicted protein-encoding genes.The Rc207 genome encodes a large and diverse set of genes involved in pathogenicity,especially rich in those encoding secreted proteins,carbohydrateactive enzymes(CAZymes),peptidases,nucleases,cytochrome P450,and secondary metabolismassociated enzymes.Most secretory protein-encoding genes,including CAZymes,peroxygenases,dehydrogenases,and cytochrome P450,were up-regulated during fungal infection of wheat.We identified 831 candidate secretory effectors and validated the functions of 10 up-regulated candidate effector proteins.Of them,nine were confirmed as necrotrophic pathogen’s effectors promoting fungal infection.Abundant potential mobile or plastic genomic regions rich in repeat sequences suggest their roles in fungal adaption and virulence-associated genomic evolution.This study provides valuable resources for further comparative and functional genomics on important fungal pathogens,and provides essential tools for development of effective disease control strategies.
基金Supported by Young Academic Backbone Support Program of Heilongjiang Province(1152G022)~~
文摘[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by reverse transcription-polymerase chain reaction（RT-PCR）.Then RT-PCR products were cloned into the PMD18-T vector and sequenced.The functions of the sequence were predicted with bioinformatics method.[Result] A 1 079 bp gene was obtained.The gene encoded a protein with 236 amino acids.The protein contains many motif sites,two WRKY domains and a C2H2 zinc finger motif.The gene showed high identities with WRKY8,WRKY24 and WRKY30 gene of rice.[Conclusion] The up-regulated expression gene induced by R.solani was representative WRKY family gene.The gene could play an important role on rice sheath blight resistance.
文摘本研究采用平板对峙法、菌丝生长速率法和菌核萌发抑制法筛选出一株细菌LB6-2,对立枯丝核菌的菌丝生长抑制率为71.76%,菌核萌发抑制率为100.00%。通过观察形态学特征、分析理化性质结合16S r DNA扩增序列分析对该菌株鉴定为萎缩芽胞杆菌Bacillusatrophaeus(OK639005.1)。采用大孔树脂法提取发酵产物并进行HPLC制备,对获得的14个组分进行活性测定确定其活性组分,从活性组分中制备获得3个单品化合物LBN4-2、LBN4-4、LBN4-5,经HPLC-MS、NMR分析和活性测定结果表明,化合物LBN4-2为Cyclo(Pro-Val)、LBN4-4为Cyclo(D-Leu-L-Pro)和LBN4-5为Cyclo(L-prolyl-D-phenylalanyl),3个化合物的浓度在4 mmol/L时对立枯丝核菌菌丝生长的抑制率分别为86.16%、84.87%和83.10%,浓度在2 mmol/L以上时,对立枯丝核菌菌核萌发的抑制率为100%。由此可见,萎缩芽胞杆菌LB6-2具有作为防治立枯丝核菌生防菌剂研发的潜力。
文摘分别基于7个烟草靶斑病菌株基因组中ITS-5.8S r DNA序列设计引物和探针;并对引物及探针特异性进行验证;建立检测体系并对接种烟草靶斑病菌的叶片和土壤中的烟草靶斑病菌进行检测。结果表明:所设计的引物及探针对R.solani AG-3具有特异性,检测体系可以检测出烟草叶片及土壤样品中的烟草靶斑病菌。接种烟草叶片的检测表明,接种后6h就可检测到强致病力菌株YC-9,12h后能检测到弱致病力菌株LF-2;获得了烟草靶斑病菌DNA质量的对数与添加菌丝量的对数之间的回归曲线方程,对不同月份土壤样品的测定结果表明烟草靶斑病菌在土壤中呈周年动态变化趋势。
