27 Rhizoctonia solani isolates from carnation with damping-off symptoms were identified as anastomosis group AG-4 based on cultural characteristics and anastomosis in Yunnan.Sequence analysis of 5.8S rDNA-ITS of yx an...27 Rhizoctonia solani isolates from carnation with damping-off symptoms were identified as anastomosis group AG-4 based on cultural characteristics and anastomosis in Yunnan.Sequence analysis of 5.8S rDNA-ITS of yx and tx presented 99% sequence similarity with AG-4 HGⅠtester isolate.Pathogenicity tests showed that isolates of AG-4 HG Ⅰ were pathogenic to carnation.This is the first record of damping-off of carnation caused by AG-4 HG Ⅰ in China.展开更多
Sixty-five samples were collected from rhizosphere soil,hot pepper and tomato plants showing damping-off,root rot and stem rot in Taian,Shouguang of Shandong province and Zhouzhi,Taibai of Shaanxi province.Thirty-nine...Sixty-five samples were collected from rhizosphere soil,hot pepper and tomato plants showing damping-off,root rot and stem rot in Taian,Shouguang of Shandong province and Zhouzhi,Taibai of Shaanxi province.Thirty-nine Rhizoctonia solani isolates were obtained from these samples.The results of anastomosis group(AG) identification and sequence analysis of 5.8S rDNA-ITS of the isolates showed that thirty-six isolates(92.3%) belonged to AG-4,while only three(7.7%) belonged to AG-5.The isolates of AG-4 could further be divided into two subgroups of AG-4-HG-Ⅰ and AG-4-HG-Ⅲ.The 5.8S rDNA-ITS sequences of the selected isolates of the two subgroups had the 99%-100% identity with standard isolates of AG-4-HG-Ⅰ and AG-4-HG-Ⅲ(from GenBank).Among the analyzed isolates,AG-4-HG-Ⅰ subgroup was the dominant with the frequency of 79.5%.Subgroup AG-4-HG-Ⅲ with the frequency of 12.8% was the second.This is the first report that subgroup AG-4-HG-Ⅲ of R.solani isolated from Solanaceae vegetable crops in China.展开更多
文摘27 Rhizoctonia solani isolates from carnation with damping-off symptoms were identified as anastomosis group AG-4 based on cultural characteristics and anastomosis in Yunnan.Sequence analysis of 5.8S rDNA-ITS of yx and tx presented 99% sequence similarity with AG-4 HGⅠtester isolate.Pathogenicity tests showed that isolates of AG-4 HG Ⅰ were pathogenic to carnation.This is the first record of damping-off of carnation caused by AG-4 HG Ⅰ in China.
文摘Sixty-five samples were collected from rhizosphere soil,hot pepper and tomato plants showing damping-off,root rot and stem rot in Taian,Shouguang of Shandong province and Zhouzhi,Taibai of Shaanxi province.Thirty-nine Rhizoctonia solani isolates were obtained from these samples.The results of anastomosis group(AG) identification and sequence analysis of 5.8S rDNA-ITS of the isolates showed that thirty-six isolates(92.3%) belonged to AG-4,while only three(7.7%) belonged to AG-5.The isolates of AG-4 could further be divided into two subgroups of AG-4-HG-Ⅰ and AG-4-HG-Ⅲ.The 5.8S rDNA-ITS sequences of the selected isolates of the two subgroups had the 99%-100% identity with standard isolates of AG-4-HG-Ⅰ and AG-4-HG-Ⅲ(from GenBank).Among the analyzed isolates,AG-4-HG-Ⅰ subgroup was the dominant with the frequency of 79.5%.Subgroup AG-4-HG-Ⅲ with the frequency of 12.8% was the second.This is the first report that subgroup AG-4-HG-Ⅲ of R.solani isolated from Solanaceae vegetable crops in China.
