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Rapid analysis of components in Rhizoma Anemarrhenae by HPLC-DAD-MS and HPLC-DAD-TOFMS
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作者 Ke-Ren Wang1,Hai Zhang2,Zhen-Yu Zhu1,Zi-Yang Lou2,Liang Zhao1,Yi-Feng Chai1 1.Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433 2.Department of Pharmacy,Eastern Hepatobiliary Surgery Hospital,Shanghai 200438,China 《Journal of Pharmaceutical Analysis》 SCIE CAS 2010年第3期149-155,共7页
A global quality control method based on high performance liquid chromatography(HPLC)coupled with diode array detection(DAD),single quadrupole mass spectrometry(MS)and time-of-flight mass spectrometry(TOFMS)was develo... A global quality control method based on high performance liquid chromatography(HPLC)coupled with diode array detection(DAD),single quadrupole mass spectrometry(MS)and time-of-flight mass spectrometry(TOFMS)was developed for simultaneous determination of seven major components(mangiferin,neomangiferin,timosaponin E1,timosaponin E,timosaponin BⅡ,timosaponin BⅢ,and timosaponin AⅢ)and identification of most components in extracts of Rhizoma Anemarrhenae(RA).HPLC analysis was performed on an Agilent SB-C18 column(4.6 mm×150 mm,5 μm)by gradient elution using acetonitrile and water-acetic acid(100∶0.05,v/v)as the mobile phase.Seven major components in RA were successfully separated.This quantitative method was fully validated in respect of the following performance criteria:linearity,precision,repeatability,stability,accuracy,limits of detection(LOD)and quantification(LOQ).A formula database of known compounds in RA was established,against which,most of the reported components in this herbal extract were identified effectively based on the extract masses acquired by TOFMS.This qualitative and quantitative method was successfully used to analyze the components in 10 batches of RA samples collected from different regions in China.This global quality control method,which consisted of HPLC-DAD-MS assay of seven major components and unambiguous identification of nineteen components,is suitable for routine quantification and comprehensive quality control of RA. 展开更多
关键词 HPLC-DAD HPLC-MS HPLC-TOFMS rhizoma anemarrhenae simultaneous determination IDENTIFICATION
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Study on the effect of Rhizoma Anemarrhenae on glucose and lipid metabolism in diabetic mice
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作者 Jin-Bao Zhang Peng-Wei Zhuang +2 位作者 Qian Wang Chun-Yu He Yan-Jun Zhang 《TMR Pharmacology Research》 2021年第1期28-34,共7页
Objective:To investigate the effect of Rhizoma Anemarrhenae extract on glucose and lipid metabolism in diabetic mice,and provide basis for follow-up research.Methods:The method of streptozotocin combined with high-fat... Objective:To investigate the effect of Rhizoma Anemarrhenae extract on glucose and lipid metabolism in diabetic mice,and provide basis for follow-up research.Methods:The method of streptozotocin combined with high-fat diet was used to replicate the diabetes model in this study.After continuous intragastric administration for 6 weeks,the effects of Rhizoma Anemarrhenae extract on body weight,blood sugar,blood lipids,serum insulin and the organ index in diabetic mice were detected.Results:The fasting blood glucose and blood lipids(serum total cholesterol,triglyceride,low density lipoprotein cholesterol levels)of the Rhizoma Anemarrhenae group of mice were significantly decreased,which was statistically significant compared with the model group.The area under the curve of the Rhizoma Anemarrhenae group is significantly smaller than that of the model group,and the result is statistically significant.Rhizoma Anemarrhenae can increase serum insulin levels in diabetic mice,which is statistically significant compared with the model group.Compared with the model group,the liver,spleen,kidney and pancreas indexes of the Rhizoma Anemarrhenae group are significantly reduced.Conclusion:The extract of Rhizoma Anemarrhenae can significantly reduce blood glucose concentration,lower blood lipid levels,promote serum insulin secretion,and protect liver,kidney,spleen,pancreas and other tissues and organs. 展开更多
关键词 rhizoma anemarrhenae Diabetic mice Blood glucose Blood lipids
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仙灵骨葆胶囊TLC鉴别方法研究
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作者 祝清灿 徐东 +4 位作者 汪娥 孙宜春 段丽 李慧馨 胡晓 《中国民族民间医药》 2022年第14期53-56,共4页
目的:提高仙灵骨葆胶囊质量标准。方法:采用薄层色谱法对仙灵骨葆胶囊中淫羊藿、续断、丹参、补骨脂、知母进行鉴别。结果:薄层色谱斑点清晰,阴性对照无干扰。结论:建立的方法专属性强,重现性好,可作为仙灵骨葆胶囊的定性鉴别。
关键词 仙灵骨葆胶囊 薄层色谱 淫羊藿 续断 丹参 补骨脂 知母
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The structural elucidation of two new artificial steroidal saponins 被引量:2
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作者 Bin Wu Zhi Yu Liu +3 位作者 Ming Song Fan Zhao Lin Sun Wei Xin Jiang Cheng Gang Huang 《Chinese Chemical Letters》 SCIE CAS CSCD 2012年第3期332-334,共3页
Two novel steroidal saponins(timosaponin BⅢ-a,timosaponin BⅢ-b) together with a known steroidal saponin(timosaponin BⅡ-b) were prepared by the dilute acid hydrolysis of timosaponin BⅢ.Their structures were elu... Two novel steroidal saponins(timosaponin BⅢ-a,timosaponin BⅢ-b) together with a known steroidal saponin(timosaponin BⅡ-b) were prepared by the dilute acid hydrolysis of timosaponin BⅢ.Their structures were elucidated by means of 1D,2D NMR and TOF-MS spectral analysis. 展开更多
关键词 rhizoma anemarrhenae Steroidal saponin Timosaponin BⅢ Acid hydrolysis NMR
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Comprehensive Metabolic Profiling of Modified Gegen Qinlian Decoction by Ultra-high-Performance Liquid Chromatography-Diode Array Detection-Q-Exactive-Orbitrap-Electrospray Ionization-Mass Spectrometry/Mass Spectrometry and Application of High-Performance 被引量:1
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作者 Xuehong Nöst Eva-Maria Pferschy-Wenzig +3 位作者 Xiao-Tong Yu Min Li Xiao-Lin Tong Rudolf Bauer 《World Journal of Traditional Chinese Medicine》 2021年第1期11-32,共22页
Objective:Gegen Qinlian decoction(GQD)is a classical traditional Chinese medicine formulation which has been used for almost 2000 years.At Guang'anmen Hospital,Beijing,a modified GQD version(m GQD)with seven inste... Objective:Gegen Qinlian decoction(GQD)is a classical traditional Chinese medicine formulation which has been used for almost 2000 years.At Guang'anmen Hospital,Beijing,a modified GQD version(m GQD)with seven instead of four herbal ingredients has been applied to treat Type 2 diabetes.Quality control is a crucial prerequisite for the therapeutic application of herbal medicines.For the identification of products derived from classical GQD,the Chinese Pharmacopeia requires the analysis of only three marker compounds.Because m GQD is a more complex mixture containing seven herbs and hundreds of constituents,the pharmacopoeia method for GQD is inadequate.Materials and Methods:A more comprehensive characterization of the formula's constituents has been developed using ultra-high-performance liquid chromatography-diode array detection(UHPLC-DAD)-Q-Exactive-mass spectrometry(MS)in electrospray ionization positive and negative mode.Moreover,a new method for the fingerprint analysis of m GQD via high-performance thin-layer chromatography(HPTLC)has been established.Results:Altogether,91 compounds have been assigned to their originating plants and 84 substances were identified either by comparison with authentic references or with data from the literature.The HPTLC method is based on the application of two different mobile phases and is able to detect both lipophilic and hydrophilic constituents of m GQD.Conclusions:The modified GQD was extensively characterized by UHPLC combined with DAD and Q-Exactive Orbitrap high-resolution MS detection,leading to the assignment and identification of compounds present in the decoction.In addition,a new method for the fingerprint analysis of the m GQD using HPTLC was established,which allows fast and simple identification of the herbal ingredients in the mixture. 展开更多
关键词 Fructus jujubae HPTLC Modified gegen qinlian decoction Radix et rhizoma glycyrrhizae praeparata cum melle Radix puerariae lobatae Radix scutellariae rhizoma coptidis rhizoma anemarrhenae rhizoma zingiberis UHPLC-DAD-Q-Exactive-MS
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