ExperimentalStudyofRPlasmidEliminatingActionofRhizomaCoptidisonE．Coli

The R plasmid curing experiment was performed in vitro with E. coli strain E. 102 bearing Rplasmid with multiple drug resistance as target bacteria and Rhizoma Coptidis as elimination agent, and theinfluence at different acting time of it on R plasmid elimination was also observed. Results showed that whenthe acting time was 24 hours, the cure rate of R plasmid was 2 . 42 % and when the action time increased to 48hours, the cure rate was elevated to 22. 57% . The missing pattern s of R plasmid might occur in diap-pearence of either multiple or single resistance.

黄芩和黄连对大肠杆菌R质粒消除作用的实验研究

目的：研究黄芩和黄连对大肠杆菌Ｒ质粒的消除作用。方法：以黄芩和黄连的提取物为质粒消除剂，携带Ｒ质粒的多重耐药性大肠杆菌Ｅ．１０２株为靶细菌，采用倍比稀释法和影印培养法及Ｋａｄｏ法，观察黄芩和黄连作用不同时间对大肠杆菌Ｒ质粒表达的影响。结果：黄芩和黄连作用２４ｈ，对大肠杆菌Ｒ质粒的消除率为５％～２．４２％，延长时间至４８ｈ，消除率为２６．１４％～２２．５７％。两个时间的消除率差异有显著意义（Ｐ＜０．０１）。Ｒ质粒消除表型可以表现为单一或两种耐药性的丢失。