[Objective] To investigate the prevention and control effect of polysaccharide from the rhizomorph of Armillaria mellea by distilled water elution （AMP-1） on diabetic cataract in rats.[Methods] Streptozotocin （STZ...[Objective] To investigate the prevention and control effect of polysaccharide from the rhizomorph of Armillaria mellea by distilled water elution （AMP-1） on diabetic cataract in rats.[Methods] Streptozotocin （STZ）,combined with freund＇s adjuvant （CFA）,was used to induce diabetic cataract in rat,to observe the onset of lenticular turbidity by slit lamp and compare the turbid degree of eyes lens among different groups.The super oxide dismutase （SOD）,glutathione peroxidase （GSH-px） and malondialdehyde （MDA） in serum and eyes lens were measured by colorimetric method.[Results] The slit lamp examination results showed that AMP-1 obviously delayed the onset of diabetic cataract and reduced turbid degree of eyes lens.Compared with model group,AMP-1 at various doses should significantly reduce the level of MDA ascended in serum of rats.Medium-and high-dose groups could increase the levels of SOD and GSH-Px in serum,and the dose of AMP-1 was closely related to the effect.[Conclusion] AMP-1 played a prominent role in prevention and treatment of diabetic cataract in rats.展开更多
The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tre...The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.展开更多
[Objectives]This study was conducted to optimize the germplasm resources of Gastrodia elata and improve the condition of declining G.elata production.[Methods]A scientific and reasonable method was used to screen the ...[Objectives]This study was conducted to optimize the germplasm resources of Gastrodia elata and improve the condition of declining G.elata production.[Methods]A scientific and reasonable method was used to screen the optimum nutritional conditions(carbon sources,nitrogen sources,microelements,vitamins)and environmental conditions(pH,temperature,light)of Armillaria mellea A9 growth,so as to screen the medium suitable for the growth of A.mellea.[Results]Under the semi-solid culture condition,the suitable pH range of A.mellea mycelia was 4.0-9.0;the temperature was about 25℃;and the light condition was dark culture.Under the experimental conditions,the optimal carbon source was 25 g/L ethanol;the optimal nitrogen source was 2.5 g/L soy peptone;and the optimum microelement and vitamin were 0.5 g/L MnSO 4,0.5 g/L MgSO 4 and 20 mg/L vitamin B6,respectively.After screening,the growth rate of A.mellea rhizomorph was as high as 13.52 mm/d,and the dry weight of mycelium also reached 0.296 7 g.[Conclusions]This study not only omitted a lot of tedious experiments,but also obtained reliable and scientific experimental results,and achieved the purpose of rejuvenating A.mellea,which is conducive to the further development and utilization of G.elata and its symbiotic fungi.展开更多
基金Supported by Scientific Research Fund of Jianghuai College of Anhui University(2012KJ0004)~~
文摘[Objective] To investigate the prevention and control effect of polysaccharide from the rhizomorph of Armillaria mellea by distilled water elution （AMP-1） on diabetic cataract in rats.[Methods] Streptozotocin （STZ）,combined with freund＇s adjuvant （CFA）,was used to induce diabetic cataract in rat,to observe the onset of lenticular turbidity by slit lamp and compare the turbid degree of eyes lens among different groups.The super oxide dismutase （SOD）,glutathione peroxidase （GSH-px） and malondialdehyde （MDA） in serum and eyes lens were measured by colorimetric method.[Results] The slit lamp examination results showed that AMP-1 obviously delayed the onset of diabetic cataract and reduced turbid degree of eyes lens.Compared with model group,AMP-1 at various doses should significantly reduce the level of MDA ascended in serum of rats.Medium-and high-dose groups could increase the levels of SOD and GSH-Px in serum,and the dose of AMP-1 was closely related to the effect.[Conclusion] AMP-1 played a prominent role in prevention and treatment of diabetic cataract in rats.
基金Supported by Key Project of Natural Sciences of Sichuan Provincial Department of Education(12ZA103)
文摘The effects of the tree species and the dosages of Armillariella mellea rhizomoph wood on asexual propagation of wild Gastrodia elata Bl. f. glauca S. Chow were studied in Ganzi prefecture. The results showed that tree species had a very significant effect on asexual reproduction yield of wild Gastrodia elata Bl. f. glauca S. Chow. The drying rate and yield of Gastrodia elata Bl. f. glauca S. Chow cultured by close grained woods such as Quercus spinosa David ex Fr. was extremely significant higher than that cultured by coarse grained wood such as Betula platyphylla Suk. The dosage had a very significant effect on the yield of wild Gastrodia elata Bl. f. glauca S. Chow whereas it had no influence on the drying rate.
文摘[Objectives]This study was conducted to optimize the germplasm resources of Gastrodia elata and improve the condition of declining G.elata production.[Methods]A scientific and reasonable method was used to screen the optimum nutritional conditions(carbon sources,nitrogen sources,microelements,vitamins)and environmental conditions(pH,temperature,light)of Armillaria mellea A9 growth,so as to screen the medium suitable for the growth of A.mellea.[Results]Under the semi-solid culture condition,the suitable pH range of A.mellea mycelia was 4.0-9.0;the temperature was about 25℃;and the light condition was dark culture.Under the experimental conditions,the optimal carbon source was 25 g/L ethanol;the optimal nitrogen source was 2.5 g/L soy peptone;and the optimum microelement and vitamin were 0.5 g/L MnSO 4,0.5 g/L MgSO 4 and 20 mg/L vitamin B6,respectively.After screening,the growth rate of A.mellea rhizomorph was as high as 13.52 mm/d,and the dry weight of mycelium also reached 0.296 7 g.[Conclusions]This study not only omitted a lot of tedious experiments,but also obtained reliable and scientific experimental results,and achieved the purpose of rejuvenating A.mellea,which is conducive to the further development and utilization of G.elata and its symbiotic fungi.
