Optimizing Efficient Micropropagation System of Rhododendron Brachycarpum D. Don by Uniform Design Method

[Objective] The study was conducted to establish a high efficient micropropagation system for Rhododendron Brachycarpum D. Don and realize the high efficiency in vitro micropropagation of R. brachycarpum. [ Method ] Young stems of R. brachycarpum were used as explants, suitable medium compositions for axillary bud growing and rooting were screened through uniform design exPeriments. [ Result] MS （modified） ＋ IAA 0,15 mg/ L ＋ IBA 0.30 mg/L ＋ GA3 3.00 mg/L was the most suitable medium with the regeneration rate of 92%. Stems each with one node were cut from regenerated shoots and cultured for propagation, the proliferative multiple was over 45 within one culture period of 35 d. [ Conclusion] High efficient micropropagation system of R. brachycarpum has been successfully established, which provides some basis for development and utilization and industrial seedling growth of the alpine rhododendron in Changbai Mountain northeast China.

均匀设计法优化短果杜鹃高效快繁体系

[目的]建立短果杜鹃高效快繁体系,实现短果杜鹃的高效离体快繁。[方法]以短果杜鹃嫩茎段为外植体,应用均匀设计法筛选最适合的培养基。[结果]最适合嫩茎段的腋芽萌发及生根的最佳培养基为MS(改良)+IAA0.15mg/L+IBA0.30mg/L+GA33.00mg/L,再生率达92%以上。以再生植株的茎节为材料进行快繁的结果表明,在35d的1个培养周期内增殖倍数平均达45以上。[结论]该研究建立了短果杜鹃的高效快繁体系,为长白山高山杜鹃的开发利用和工厂化育苗提供了依据。

藏药髯花杜鹃花中化学成分的研究

目的研究藏药髯花杜鹃Rhododendron anthopogonD.Don花中的化学成分。方法采用95%乙醇提取制备总浸膏,经萃取及柱层析分离,核磁共振鉴定结构。结果分离得14个已知化合物,分别为三油酸甘油酯、3-羟基-3-苯基丙酰胺、齐墩果酸甲酯、熊果酸、2-羟基-4-甲氧基苯酚、5-甲氧基糠醛、7-羟基香豆素、23-羟基乌苏酸、β-谷甾醇、槲皮素、杨梅素、桃叶珊瑚苷、杨梅素-3′-O-β-D-吡喃木糖苷、金丝桃苷。结论所有化合物均为首次从该植物花中分离得到。