DNA methylation is a kind of important epigenetic phenomenon,which could regulate gene expression in cells by affecting the transcription and duplication of DNA without changing cell DNA base sequence. Azalea is one o...DNA methylation is a kind of important epigenetic phenomenon,which could regulate gene expression in cells by affecting the transcription and duplication of DNA without changing cell DNA base sequence. Azalea is one of the ten famous flowers in China,as well a famous flower in the world,and the whole plant has medicinal value. This study investigated the change profile of gene methylation during the four flowering stages of Rhododendron pulchrum by methylation sensitive amplification polymorphism（ MSAP）,and 412 differential bands were obtained after selective amplification with screened 18 pairs of effective primers. Among the differential bands,23 differential bands were selected and subjected to recovery,sequencing and data alignment,and it was found that chalcone synthase,cowpox mitochondria,birch liana mitochondria,HDR reductase in camellia,Vaccinium macrocarpon mitochondria,Cyperus rotundus mitochondria protein,Shigella flexneri plasmid and Scophthalmus maximus cD NA-AFLP fragment have a regulating effect on azalea epigenetic inheritance,and next,genes related to the flowering period of R. pulchrum would be further studied,thereby improving the economic value of R. pulchrum.展开更多
将BP人工神经网络技术与传统的正交试验方法相结合,提出一种新的试验分析和处理方法,利用神经网络特有的自学能力,对主要影响因素进行仿真优化,获得锦锈杜鹃黄酮物质提取的最佳工艺:料液比1∶20(g/mL),超声波提取时间为40 m in,提取温度...将BP人工神经网络技术与传统的正交试验方法相结合,提出一种新的试验分析和处理方法,利用神经网络特有的自学能力,对主要影响因素进行仿真优化,获得锦锈杜鹃黄酮物质提取的最佳工艺:料液比1∶20(g/mL),超声波提取时间为40 m in,提取温度为60℃,乙醇质量分数为40%.应用优化得到的工艺条件进行验证试验。展开更多
基金Supported by Open fund of Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization(2017BX06)Fund of College students'Innovative and Entrepreneurial Activities of Huanggang Normal University
文摘DNA methylation is a kind of important epigenetic phenomenon,which could regulate gene expression in cells by affecting the transcription and duplication of DNA without changing cell DNA base sequence. Azalea is one of the ten famous flowers in China,as well a famous flower in the world,and the whole plant has medicinal value. This study investigated the change profile of gene methylation during the four flowering stages of Rhododendron pulchrum by methylation sensitive amplification polymorphism（ MSAP）,and 412 differential bands were obtained after selective amplification with screened 18 pairs of effective primers. Among the differential bands,23 differential bands were selected and subjected to recovery,sequencing and data alignment,and it was found that chalcone synthase,cowpox mitochondria,birch liana mitochondria,HDR reductase in camellia,Vaccinium macrocarpon mitochondria,Cyperus rotundus mitochondria protein,Shigella flexneri plasmid and Scophthalmus maximus cD NA-AFLP fragment have a regulating effect on azalea epigenetic inheritance,and next,genes related to the flowering period of R. pulchrum would be further studied,thereby improving the economic value of R. pulchrum.
文摘将BP人工神经网络技术与传统的正交试验方法相结合,提出一种新的试验分析和处理方法,利用神经网络特有的自学能力,对主要影响因素进行仿真优化,获得锦锈杜鹃黄酮物质提取的最佳工艺:料液比1∶20(g/mL),超声波提取时间为40 m in,提取温度为60℃,乙醇质量分数为40%.应用优化得到的工艺条件进行验证试验。
