Dynamic Changes in Endogenous Hormones in Rhododen- dron simsii Floral Organs

This study was conducted to investigate the dynamic changes of several hormones in Rhododendron simsii floral organs to provide theoretical basis for controlling its flowering period. A R. simsii variety ＂Purple Crane＂ was selected as the experimental material, and its flower organs were sampled at nine floral developmental stages to measure the contents of gibberellic acid-3 （GA3 ）, abscisic acid （ABA）, indole-3-acetic acid （IAA）, and zeatin riboside （ZR） through enzyme-linked immunosorbent assay （ELISA）. The results showed that the content of ZR was highest at full flowering stage; IAA and GA3 contents were higher than other stages in the process of flower bud differentiation and flowering. ABA played an important role in the regulation of flower bud formation. The results will provide theoretical references for exploring the regulation of plant hormones towards Rhododendron flower traits and breeding new varieties.

不同花色映山红花中熊果酸和齐墩果酸的含量比较

结合超声技术提取不同花色映山红花中熊果酸和齐墩果酸,建立RP-HPLC法测定其含量。采用Kromasil C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸水溶液(88∶12),光电二极管阵列检测器,检测波长210nm,流速0.8mL/min,柱温30℃。以保留时间和紫外光谱对分离出的组分予以定性确证,用峰面积进行定量。组分的质量浓度与其峰面积在一定的范围内呈现良好的线性关系,相关系数均为0.9999,熊果酸进样量在0.443～7.088μg,齐墩果酸进样量在0.247～3.952μg时呈现良好的线性关系,平均加样回收率分别为98.53%和98.36%,RSD分别为1.3%和1.5%(n=5)。方法灵敏、准确,重现性好,可用于不同花色映山红花中熊果酸和齐墩果酸含量的测定。

屋顶花园构造及几个关键环节的处理

从屋顶花园的构造出发,结合屋顶花园的功能、国内外发展现状和趋势,讨论和阐述了屋顶花园建设过程中荷载、培养基质、防水防风和使用安全等几个关键环节出现问题的原因,提出了解决这些问题的对策。

修剪对野生杜鹃开花的影响

研究了不同修剪方法对野生杜鹃小年开花和枝条生长之间的影响。结果表明:花芽数量和开花数量,短截、摘花和轻回缩与对照存在极显著差异;枝条生长量,摘花、短截、轻回缩、中回缩和重回缩与对照存在极显著差异;摘花和短截既有利于野生杜鹃枝条生长量的增加,还能有效促进花芽分化和开花,花芽数量分别达64.6个/m2和72.8个/m2,开花数量分别达60.8朵/m2和68.5朵/m2。

修剪强度对野生杜鹃生长开花的影响

对野生杜鹃采取不同修剪强度的对比试验,结果表明:重回缩使野生杜鹃枝条平均生长量能达到40 cm,有利于枝条更新复壮;摘花使野生杜鹃抽出枝条达603条/m2,摘花有利于枝条抽出;短截枝条生长量5 843 cm/m2,短截能有效促进枝条生长量增加;短截使杜鹃花芽72.8个/m2,中回缩和重回缩花芽为0;短截杜鹃开花68.5朵/m2,开花数量最多。