Isolation and pharmacological activities of bromophenols from Rhodomela confervoides

Four bromophenols were isolated from the extract of marine red alga, Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods including IR, MS, 1D, and 2D-NMR techniques, their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl) ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549 cells, 2 showed powerful antibacterial activities against Staphylococcus aurens and Pseudomonas aeruginosa.

海洋红藻松节藻Rhodomela confervoides化学成分研究

目的:对采自青岛海滨的海洋红藻松节藻 Rhodomela confervoides进行化学成分研究。方法:各种色谱柱层析和IR、MS、~1H-NMR、^(13)C-NMR、DEPT等波谱解析技术。结果:从松节藻中分离得到5个化合物,其结构经过鉴定分别为:2,2′,3,3′-四溴-4,4′,5,5′-四羟基双苯基甲烷(Ⅰ)、2,2′,3-三溴-3′,4,4′,5-四羟基-6′-甲氯甲基双苯基甲烷(Ⅱ),2,3-二溴-4,5-羟基苯甲醇(Ⅲ)、2,3-二溴-4,5-二羟基-苯甲醛(Ⅳ)、2,3-二溴-4,5-二羟基苯甲基已基醚(Ⅴ)。其中化合物Ⅱ对KB和Be17402肿瘤细胞有选择性细胞毒活性。