E．coli核糖体蛋白质S12依赖链霉素突变抑制λN基因表达的分子机制

本文利用ＰＣＲ方法克隆了Ｅ．ｃｏｌｉＴ８３依赖链霉素（ｓｔｅｒｐｔｏｍｙｅｉｎｄｅｐｅｎｄｅｎｔ，Ｓｍ＾ｄ）菌株中编码突变的核糖体蛋白质Ｓ１２的ｒｐｓＬ＾ｄ基因，并进行了ＤＮＡ序列分析，发现第４２位密码子由编码赖氨酸（Ｌｙｓ，Ｋ）的ＡＡＡ变为编码谷氨酰胺（Ｇｌｎ，Ｑ）的ＣＡＡ。依据Ｇａｒｎｉｅｒ原理预测了突变对Ｓ１２蛋白质二级结构形成趋势的影响，结果表明。

RIBOSOMAL PROTEIN S12 GENE MUTATION AFFECTING THE EXPRESSION OF BACTERIOPHAGE LAMBDA N GENE

Previous results in this laboratory indicated that in ribosomal protein S12 strepto-mycin-dependent mutants of Bacillus subtilis, the burst size of bacteriophage φ105 was decreased, and the protein synthesis was inhibited, while the DNA and