Seeds of japonica rice cv.Zhenuo 2 with twodifferent physiological states(dry seeds withwater content 13% and wet seeds soaked in thewater for 36 h)were irradiated by COlaser infour different power-densities and durat...Seeds of japonica rice cv.Zhenuo 2 with twodifferent physiological states(dry seeds withwater content 13% and wet seeds soaked in thewater for 36 h)were irradiated by COlaser infour different power-densities and durations re-spectively.The treatment irradiated with 200GY ofCo γ-rays was considered as control.The flesh root tips were cut and fixed inCarnoy’s fluid for cytological examination.展开更多
The seedlings of three rice varieties (Damagu,Ligeng 2, and Xiuzinuo) were cultivated at 25 ±1℃. After chilling injury (at 2~C), blacksediments of lead phosphate appeared on plas-
The primary aim of the present study was to investigate the overexpression of the rice （Oryza sativa L.） programmed cell death 5 （OsPDCD5） gene in rice plant. Constitutive expression of OsPDCD5 from the cauliflowe...The primary aim of the present study was to investigate the overexpression of the rice （Oryza sativa L.） programmed cell death 5 （OsPDCD5） gene in rice plant. Constitutive expression of OsPDCD5 from the cauliflower mosaic virus （CaMV） 35S promoter induced programmed cell death （PCD） in transgenic rice. Programmed cell death was accompanied by typical features, including inhibition of developmental growth, a reduction of fresh weight, degradation of total protein content, and fragmentation of genomic DNA. These results suggest that OsPDCD5 plays an essential role in the regulation of PCD in rice plants.展开更多
Lesion mimic mutant（LMM） genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5,which displayed l...Lesion mimic mutant（LMM） genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5,which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae（Xoo） by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A（e EF1A）-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indicaspecific manner, respectively. In addition, high-throughput m RNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous e EF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice.展开更多
文摘Seeds of japonica rice cv.Zhenuo 2 with twodifferent physiological states(dry seeds withwater content 13% and wet seeds soaked in thewater for 36 h)were irradiated by COlaser infour different power-densities and durations re-spectively.The treatment irradiated with 200GY ofCo γ-rays was considered as control.The flesh root tips were cut and fixed inCarnoy’s fluid for cytological examination.
文摘The seedlings of three rice varieties (Damagu,Ligeng 2, and Xiuzinuo) were cultivated at 25 ±1℃. After chilling injury (at 2~C), blacksediments of lead phosphate appeared on plas-
文摘The primary aim of the present study was to investigate the overexpression of the rice （Oryza sativa L.） programmed cell death 5 （OsPDCD5） gene in rice plant. Constitutive expression of OsPDCD5 from the cauliflower mosaic virus （CaMV） 35S promoter induced programmed cell death （PCD） in transgenic rice. Programmed cell death was accompanied by typical features, including inhibition of developmental growth, a reduction of fresh weight, degradation of total protein content, and fragmentation of genomic DNA. These results suggest that OsPDCD5 plays an essential role in the regulation of PCD in rice plants.
基金supported by the grants from the Ministry of Science and Technology of China(No.2016YFD0101801)the Ministry of Agriculture of China(No.2014ZX08001002)the National Natural Science Foundation of China(Nos.31371590 and 31571245)
文摘Lesion mimic mutant（LMM） genes, stimulating lesion formation in the absence of pathogens, play significant roles in immune response. In this study, we characterized a rice lesion mimic mutant, lmm5,which displayed light-dependent spontaneous lesions. Additionally, lmm5 plants exhibited enhanced resistance to all of the tested races of Magnaporthe oryzae and Xanthomonas oryzae pv. oryzae（Xoo） by increasing the expression of defense-related genes and the accumulation of hydrogen peroxide. Genetic analysis showed that the lesion mimic phenotype of lmm5 was controlled by two genes, lmm5.1 and lmm5.4, which were isolated with a map-based cloning strategy. Remarkably, LMM5.1 and LMM5.4 share a 97.4% amino acid sequence identity, and they each encode a eukaryotic translation elongation factor 1A（e EF1A）-like protein. Besides, LMM5.1 and LMM5.4 were expressed in a tissue-specific and an indicaspecific manner, respectively. In addition, high-throughput m RNA sequencing analysis confirmed that the basal immunity was constitutively activated in the lmm5 mutant. Taken together, these results suggest that the homologous e EF1A-like genes, LMM5.1 and LMM5.4, negatively affect cell death and disease resistance in rice.
