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Stability Analysis of a Lignocellulose Degrading Microbial Consortium
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作者 Zheng Guo-xiang Li Jian +1 位作者 Zhou Chen-yang Chong Yu-ting 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第1期55-61,共7页
An active mesophilic lignocellulose degrading microbial consortium, designated LZF-12, was bred from humus-rich soil by successive subcultivation under facultative aerobic static condition. Batch experiments were perf... An active mesophilic lignocellulose degrading microbial consortium, designated LZF-12, was bred from humus-rich soil by successive subcultivation under facultative aerobic static condition. Batch experiments were performed to investigate the structural and functional stability of lignocellulose degradation of rice straw of 10 g · L-1. The results showed that efficient degradation of rice straw(>70%) could be achieved and acetic acid concentration accounted for over 70% of total aqueous products from different generations by microbial consortium LZF-12 within 7 days. Denaturing gradient gel electrophoresis(DGGE) and sequencing of 16 S r DNA sequences amplified from the total consortium DNA representing the presence of sequences were related to those of Clostridium, Clostridium cellulolyticum, Pseudomonas, Acetivibrio and some uncultured bacteria in LZF-12. DGGE pattern profiles from different LZF-12 generations were reproducible, suggesting the relative stabilities of the microbial community structure and succession mechanism in the established consortium. 展开更多
关键词 microbial consortium rice straw degradation STABILITY
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