Plant height is an important morphological trait that affects crop yield.Several genes related to plant height and yield have been reported in rice(Oryza sativa L.),however,the molecular mechanism underlying the regul...Plant height is an important morphological trait that affects crop yield.Several genes related to plant height and yield have been reported in rice(Oryza sativa L.),however,the molecular mechanism underlying the regulation of these traits is still not completely understood.VP64 is widely used as a transcriptional activator to investigate the biological function of genes encoding transcription factors.Here,we identified a novel bZIP transcription factor OsbZIP62 that is involved in modulating agronomic traits in rice.Overexpression of OsbZIP62-VP64(OsbZIP62V)significantly increases the plant height and yield per plant in rice.RNA-seq analysis showed that some plant height and panicle development related genes(i.e.,OsEATB,OsDSS1 and OsGA3ox2)were up-regulated in OsbZIP62V overexpressing rice plants.Besides,OsbZIP62 could also bind to the promoters of several putative target genes.These results suggested that OsbZIP62 plays a role as transcriptional regulator in regulating the expression of genes associated with agronomic traits,and OsbZIP62 fused with VP64 would be useful in crop genetic modification with improved plant architecture and yield.展开更多
Lodging is the most common constraint on grain yield of direct-seeded rice.There is limited information about lodging resistance and its related plant traits in direct-seeded and double-season rice(DDR)in Central Chin...Lodging is the most common constraint on grain yield of direct-seeded rice.There is limited information about lodging resistance and its related plant traits in direct-seeded and double-season rice(DDR)in Central China.This study aims to identify the plant traits that achieve high lodging resistance in ultrashort-duration varieties(about 95 days)of DDR.Field experiments were conducted in 2017 and 2018 in Wuxue County,Hubei Province,China,with four ultrashort-duration varieties grown under two nitrogen(N)rates.Lodging-related traits were measured on the 15 th day after heading,and yield and yield attributes were measured at maturity.The grain yield of the four varieties ranged from 4.59 to 7.61 t ha^(-1)across the two N rates,with a total growth duration of 85 to 97 days.Varietal differences in lodging index were mainly explained by the bending moment,which was closely related to plant height.Breaking resistance did not affect the lodging index significantly.Shortening plant height from 95.4 to 80.5 cm decreased the lodging index by 22.4%but did not reduce grain yield.Our results suggested that reducing plant height was effective in improving the lodging resistance of ultrashort-duration varieties of DDR.Lodging resistance should be enhanced by improving breaking resistance rather than reducing plant height to increase DDR grain yield further.展开更多
Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present stud...Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present study,we characterized a rice gene, ABNORMAL FLOWER AND DWARF1（AFD1） that determined plant height, floral development and grain yield. The afd1 mutant showed variable defects including the dwarfism, long panicle, low seed setting and reduced grain yield. In addition, abnormal floral organs were also observed in the afd1 mutant including slender and thick hulls, and hull-like lodicules.AFD1 encoded a DUF640 domain protein and was expressed in all tested tissues and organs. Subcellular localization showed AFD1-green fluorescent fusion protein（GFP） was localized in the nucleus. Meantime, our results suggested that AFD1 regulated the expression of cell division and expansion related genes.展开更多
基金Funding for Basic Research Projects of Taicang(TC2021JC12)School-Level Research Projects of Yancheng Institute of Technology(xjr2021029)+1 种基金Shanghai Agriculture Applied Technology Development Program(2017-02-08-00-08-F00071)Natural Science Foundation of Shanghai(18ZR1433300)。
文摘Plant height is an important morphological trait that affects crop yield.Several genes related to plant height and yield have been reported in rice(Oryza sativa L.),however,the molecular mechanism underlying the regulation of these traits is still not completely understood.VP64 is widely used as a transcriptional activator to investigate the biological function of genes encoding transcription factors.Here,we identified a novel bZIP transcription factor OsbZIP62 that is involved in modulating agronomic traits in rice.Overexpression of OsbZIP62-VP64(OsbZIP62V)significantly increases the plant height and yield per plant in rice.RNA-seq analysis showed that some plant height and panicle development related genes(i.e.,OsEATB,OsDSS1 and OsGA3ox2)were up-regulated in OsbZIP62V overexpressing rice plants.Besides,OsbZIP62 could also bind to the promoters of several putative target genes.These results suggested that OsbZIP62 plays a role as transcriptional regulator in regulating the expression of genes associated with agronomic traits,and OsbZIP62 fused with VP64 would be useful in crop genetic modification with improved plant architecture and yield.
基金supported by the National Natural Science Foundation of China(31971845)the National Key Research and Development Program of China(2016YFD0300208-03)the China Agriculture Research System of MOF and MARA(CARS-01-20)。
文摘Lodging is the most common constraint on grain yield of direct-seeded rice.There is limited information about lodging resistance and its related plant traits in direct-seeded and double-season rice(DDR)in Central China.This study aims to identify the plant traits that achieve high lodging resistance in ultrashort-duration varieties(about 95 days)of DDR.Field experiments were conducted in 2017 and 2018 in Wuxue County,Hubei Province,China,with four ultrashort-duration varieties grown under two nitrogen(N)rates.Lodging-related traits were measured on the 15 th day after heading,and yield and yield attributes were measured at maturity.The grain yield of the four varieties ranged from 4.59 to 7.61 t ha^(-1)across the two N rates,with a total growth duration of 85 to 97 days.Varietal differences in lodging index were mainly explained by the bending moment,which was closely related to plant height.Breaking resistance did not affect the lodging index significantly.Shortening plant height from 95.4 to 80.5 cm decreased the lodging index by 22.4%but did not reduce grain yield.Our results suggested that reducing plant height was effective in improving the lodging resistance of ultrashort-duration varieties of DDR.Lodging resistance should be enhanced by improving breaking resistance rather than reducing plant height to increase DDR grain yield further.
基金supported by the National Natural Science Foundation of China (31401464 and 91435105416)supported by the Open Foundation from Top Key Discipline of Modern agricultural Biotechnology and Biological Control of Crop Diseases in Zhejiang Provincial Collegethe Agricultural Sciences and Technologies Innovation Program of Chinese Academy of Agricultural Sciences (CAAS) to Rice Reproductive Developmental Biology Group
文摘Moderate plant height and successful establishment of reproductive organs play pivotal roles in rice grain production. The molecular mechanism that controls the two aspects remains unclear in rice. In the present study,we characterized a rice gene, ABNORMAL FLOWER AND DWARF1（AFD1） that determined plant height, floral development and grain yield. The afd1 mutant showed variable defects including the dwarfism, long panicle, low seed setting and reduced grain yield. In addition, abnormal floral organs were also observed in the afd1 mutant including slender and thick hulls, and hull-like lodicules.AFD1 encoded a DUF640 domain protein and was expressed in all tested tissues and organs. Subcellular localization showed AFD1-green fluorescent fusion protein（GFP） was localized in the nucleus. Meantime, our results suggested that AFD1 regulated the expression of cell division and expansion related genes.
