The diverse and contrasting effects of using human prostate cancer cell lines to study androgen receptor roles in prostate cancer

The androgen receptor （AR） plays an important role in the development and progression of prostate cancer （PCa）. Androgen deprivation therapy is initially effective in blocking tumor growth, but it eventually leads to the hormonerefractory state. The detailed mechanisms of the conversion from androgen dependence to androgen independence remain unclear. Several PCa cell lines were established to study the role of AR in PCa, but the results were often inconsistent or contrasting in different cell lines, or in the same cell line grown under different conditions. The cellular and molecular alteration of epithelial cells and their microenvironments are complicated, and it is difficult to use a single cell line to address this important issue and also to study the pathophysiological effects of AR. In this paper, we summarize the different effects of AR on multiple cell lines and show the disadvantages of using a single human PCa cell line to study AR effects on PCa. We also discuss the advantages of widely used epithelium-stroma co-culture systems, xenograft mouse models, and genetically engineered PCa mouse models. The combination of in vitro cell line studies and in vivo mouse models might lead to more credible results and better strategies for the study of AR roles in PCa.

Feasibility of College Japanese Major and Japanese Joint Training Projects

In recent years, university exchanges between China and Japan is more active, we can see that in the joint efforts of China and Japan, the scale of cooperation is expanding, understanding and friendship between the young people are gradually deepened. The long- term stability of University conducted exchanges and cooperation in education with Japan is the concrete manifestation of China＇ s opening-up. Therefore, what＇ s the meaning of joint training of undergraduate education in universities？ This paper studies on ＂2 ＋ 2＂ program between our college and the west of the international University of Japan. We analysis the favorable and unfavorable factors in joint training programs, clarify the necessity and feasibility of acting joint training programs, and provide some reference for the development of our joint training program.

Physicochemical water quality parameters in typical rice-crayfish integrated systems(RCIS)in China

To determine the variation of water quality in rice-crayfish(Procambarus clarkii)integrated systems(RCIS)in China,eleven water quality parameters were measured monthly in a typical RCIS located in Qianjiang City(Hubei Province)from July 2014 to June 2015,the parameters were analyzed with principal component analysis(PCA)and compared between the trenches and rice areas during the rice fallow period(Nov-May).The trench and rice area comprehensive results showed that pH(7.48-8.68),NH_(4)^(+)-N(0.2-1.09 mg/L),NO_(2)^(-)-N(<0.052 mg/L)and conductivity(435-951μS/cm)were within the suitable ranges for P.clarkii and that turbidity(TU)was high during the crayfish harvesting and rice planting season.Annual averages of total nitrogen(TN),total phosphorus(TP),permanganate index(CODMn),and chlorophyll a(Chl.a)content were<2(except in Nov-Dec),0.25,10 mg/L,and 50 mg/m^(3)(especially in Nov-May,<10 mg/m^(3)),respectively.Dissolved oxygen(DO)was below 4 mg/L in Mar-Sep,with a minimum of~1 mg/L,and much higher in Oct-Feb.The maximum and minimum monthly average water temperature(WT)were 31.4°C in July and 5.7°C in December,while the maximum and minimum instantaneous WT were 39.7°C and 2.5°C,respectively.PCA analysis showed that the first three axes,which were mainly correlated with DO,WT and nutrient level,described most information of the parameters,and parameters showed seasonal changes.Some differences were observed in water parameters between the trenches and rice areas,i.e.,trenches generally had higher TU,WT and DO,and lower TN,TP and CODMn,although no significant differences were found in some months and some parameters.The study revealed relatively low water nutrient level,probable extreme WT and DO level in some seasons,and certain differences between the trenches and rice areas in typical CRIS in China.Accordingly,some measures should be taken to improve the negative parameters:1)enhance the water fertility;2)increase DO,especially in Mar-Sep;3)increase the trench and water depth to avoid extreme WT.And water quality management should be addressed in both trenches and rice areas.