rimJ基因缺失不影响大肠杆菌BL21(DE3)生长的温度敏感性

目的:研究rimJ基因对大肠杆菌BL21(DE3)菌株生长的温度敏感性的影响。方法:利用SceⅠ-Red同源重组技术将大肠杆菌BL21(DE3)基因组中的rimJ基因缺失,得到rimJ缺失突变菌;比较缺失突变株和原始菌株在不同温度(25℃、37℃、42℃)下的最大比生长速率,利用统计学方法分析rimJ基因的缺失对BL21(DE3)菌株生长的温度敏感性是否有影响。结果:rimJ基因被成功敲除;统计分析结果表明缺失突变株和原始菌株的最大比生长速率没有明显区别。结论:rimJ基因缺失对大肠杆菌BL21(DE3)生长的温度敏感性无影响。

核糖体成熟因子RimP、Era和RimJ的研究进展

核糖体由小亚基和大亚基构成,它们都由rRNA和核糖体蛋白质组成。核糖体的组装和成熟是一个快速、复杂且十分耗能的细胞过程,并受到核糖体成熟(组装)因子的引导。RimP、Era和RimJ是大肠杆菌核糖体30S小亚基的成熟因子,促进30S小亚基的组装和成熟。现综述RimP、Era和RimJ在30S小亚基组装和成熟过程中的作用,为进一步揭示30S小亚基组装和成熟的过程以及RimP、Era和RimJ的功能提供依据。

RimJ-Catalyzed Sequence-Specific Protein N-Terminal Acetylation in <i>Escherichia coli</i>

In order to establish the sequence dependence of RimJ-mediated protein N-terminal acetylation in E. coli, the Z-domain variants differing by the second or third amino acid residue were expressed and analyzed by mass spectrometry. Only subsequent to the initiating methionine residue cleavage, the RimJ-catalyzed N-terminal acetylation mainly occurred at the N-terminal serine and threonine residues and was significantly enhanced by hydrophobic or negatively charged residues in the penultimate position.