Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative...Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A （CYP1A）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, catalase （CAT）, and glutathione-S-transferase （GST） in crucian carp （Carassius auratus）. The methods were then used to detect the respective mRNA expression levels in liver tissue in wild crucian carp from the Hun River, North China. CYP1A mRNA expression was significantly up-regulated in fish from stations $5, $6, and $8 （p 〈 0.05）. SOD mRNA expression was significantly down-regulated in downstream areas relative to fish from upstream sites （p 〈 0.05）; GPx and CAT mRNA expression levels were also down-regulated at $9 （p 〈 0.05）. In contrast, GST mRNA expression showed no obvious change between fish collected from up- or downstream areas of the river. Finally, an integrated biomarker response was used to evaluate the integrated impact of pollutants in the Hun River and allow better comprehension of the real toxicological risk of these investigated sites.展开更多
The rapid urbanization of China is causing a burden on their water resources and hindering their sustainable development.This paper analyzes effective methods to integrated river basin management(IRBM)using Longgang R...The rapid urbanization of China is causing a burden on their water resources and hindering their sustainable development.This paper analyzes effective methods to integrated river basin management(IRBM)using Longgang River basin of Shenzhen as an example,which is the city with the fastest rate of urbanization in China and even the whole world.Over the past 20 years,China has undergone a population boom due to the increase of immigrant workers and rapid development of laborintensive industries,which led to the sharp increase of water consumption and sewage discharge.However,the construction of the water infrastructure is still lagging far behind the environmental and social development,with only 32.7%of sewage in the district being treated.Currently,every water quality indicator of the Longgang River basin was unable to meet the required corresponding environmental standards,which further aggravated the water shortages of the region.Thus,an analytical framework is proposed to address the IRBM of the study area.The problems with the current management system include the lack of decentralization in decision-making,lack of enforcement with redundant plans,weak management capacity,financial inadequacy,and a poor system of stakeholder participation.In light of the principles of IRBM and the situation of the region,corresponding measures are put forward,including an increase of power given to sub-district offices,fewer but more feasible plans,capacity building among stakeholders,a combination of planning and marketing for overcoming financial inadequacy,and profound reform in the public participation system.The framework and institutional suggestions could inform similar processes in other representative river basins.展开更多
This paper presents the development of an urban river water quality model which considers the physical-biochemical processes within rivers and the incorporated urban catchment rainfall-runoff process developed with th...This paper presents the development of an urban river water quality model which considers the physical-biochemical processes within rivers and the incorporated urban catchment rainfall-runoff process developed with the time-area method. Unlike other models that simulate the hydrological and receiving water quality processes in the rural areas of the watershed scale, the model developed here is typically efficient for simulating the water quality response to nonpoint loadings from urban drainage systems, where the hydrological process is disturbed by artificially pumped discharge in wet-weather periods. This model is employed to assess the river water quality restoration in Nanfei River in Hefei City, China, where the model is calibrated against the measured data（i.e., the COD, the BOD5, the NH3-N, and the DO） in 2010, and the model parameters are suggested. It is shown that the nonpoint pollutants from the urban catchments contribute 34%-47% of the total pollutant inputs（i.e., the COD, the BOD5, and the NH3-N）, despite their low flow component of 13.4%. Apart from the improvement of the wastewater treatment plant effluent（i.e., Grade IV of the Surface Water Quality Standard）, a nonpoint loading reduction of 27.2%, 25.1%, and 35.3% of the COD, the BOD5, and the NH3-N are anticipated to meet the designated surface water quality standards of Grade V.展开更多
基金supported by the Water Pollution Control and Management(No.2009ZX07528)
文摘Metabolizing enzymes play important roles in the detoxification of various pollutants in aquatic organisms, thereby they can also be used to provide early-warning signals of environmental risks. Real-time quantitative reverse-transcription polymerase chain reaction assays were developed to quantify cytochrome P450 1A （CYP1A）, superoxide dismutase （SOD）, glutathione peroxidase （GPx）, catalase （CAT）, and glutathione-S-transferase （GST） in crucian carp （Carassius auratus）. The methods were then used to detect the respective mRNA expression levels in liver tissue in wild crucian carp from the Hun River, North China. CYP1A mRNA expression was significantly up-regulated in fish from stations $5, $6, and $8 （p 〈 0.05）. SOD mRNA expression was significantly down-regulated in downstream areas relative to fish from upstream sites （p 〈 0.05）; GPx and CAT mRNA expression levels were also down-regulated at $9 （p 〈 0.05）. In contrast, GST mRNA expression showed no obvious change between fish collected from up- or downstream areas of the river. Finally, an integrated biomarker response was used to evaluate the integrated impact of pollutants in the Hun River and allow better comprehension of the real toxicological risk of these investigated sites.
基金Longgang District Environmental Protection Bureau.
文摘The rapid urbanization of China is causing a burden on their water resources and hindering their sustainable development.This paper analyzes effective methods to integrated river basin management(IRBM)using Longgang River basin of Shenzhen as an example,which is the city with the fastest rate of urbanization in China and even the whole world.Over the past 20 years,China has undergone a population boom due to the increase of immigrant workers and rapid development of laborintensive industries,which led to the sharp increase of water consumption and sewage discharge.However,the construction of the water infrastructure is still lagging far behind the environmental and social development,with only 32.7%of sewage in the district being treated.Currently,every water quality indicator of the Longgang River basin was unable to meet the required corresponding environmental standards,which further aggravated the water shortages of the region.Thus,an analytical framework is proposed to address the IRBM of the study area.The problems with the current management system include the lack of decentralization in decision-making,lack of enforcement with redundant plans,weak management capacity,financial inadequacy,and a poor system of stakeholder participation.In light of the principles of IRBM and the situation of the region,corresponding measures are put forward,including an increase of power given to sub-district offices,fewer but more feasible plans,capacity building among stakeholders,a combination of planning and marketing for overcoming financial inadequacy,and profound reform in the public participation system.The framework and institutional suggestions could inform similar processes in other representative river basins.
基金Project supported by the Major Science and Technology Program for Water Pollution Control and Treatment(Grant Nos.2011ZX07303-002,2013ZX07304-002)the Shanghai Science and Technology Commission(Grant No.13DZ2251700)
文摘This paper presents the development of an urban river water quality model which considers the physical-biochemical processes within rivers and the incorporated urban catchment rainfall-runoff process developed with the time-area method. Unlike other models that simulate the hydrological and receiving water quality processes in the rural areas of the watershed scale, the model developed here is typically efficient for simulating the water quality response to nonpoint loadings from urban drainage systems, where the hydrological process is disturbed by artificially pumped discharge in wet-weather periods. This model is employed to assess the river water quality restoration in Nanfei River in Hefei City, China, where the model is calibrated against the measured data（i.e., the COD, the BOD5, the NH3-N, and the DO） in 2010, and the model parameters are suggested. It is shown that the nonpoint pollutants from the urban catchments contribute 34%-47% of the total pollutant inputs（i.e., the COD, the BOD5, and the NH3-N）, despite their low flow component of 13.4%. Apart from the improvement of the wastewater treatment plant effluent（i.e., Grade IV of the Surface Water Quality Standard）, a nonpoint loading reduction of 27.2%, 25.1%, and 35.3% of the COD, the BOD5, and the NH3-N are anticipated to meet the designated surface water quality standards of Grade V.
