日钟阴阳方对LED灯照下兔视网膜多巴胺及其D2受体水平的影响

目的：探讨日钟阴阳方对LED灯照下兔视网膜多巴胺及其D2受体水平的影响。方法：1月龄健康无眼病新西兰幼兔24只随机分成LED灯组和LED灯中药组,每组12只。再将每组分成4周组和12周组2个亚组,每组6只。LED灯组幼兔饲养在LED灯光照控制箱内,自由饮水和进食。LED灯中药组幼兔饲养环境同LED灯组,同时采用＂日钟阴阳方＂灌胃。于实验前及实验后4、8、12周测量2组幼兔体质量。测定视网膜DA及D2受体水平。结果：实验前后4组幼兔体质量均无显著变化（P〉 0. 05）。第4、12周时,LED灯中药组的视网膜DA水平显著高于LED灯组（P 〈0. 01）,视网膜D2受体蛋白在上述视网膜细胞层内的表达及D2受体表达量吸光度值强于LED灯组（P 〈0. 05或P 〈0. 01）,但4周组和12周组比较无显著差异（P〉 0. 05）。结论：在LED灯照下,日钟阴阳方可显著提高视网膜中多巴胺及其受体的表达水平,具有保护视网膜组织结构的作用,值得临床推广。

Influence of artificial luminous environment and TCM intervention on development of myopia rabbits

Objective:To explore the influence of artificial luminous environment and preventive function of traditional Chinese medicine(TCM) intervention based on "Theory of yin-yang clock" on myopia.Methods:A total of 45 New Zealand young rabbits were randomly divided into 5groups,9 for each group.Control group was exposed in natural light.Fluorescent group and full spectrum group were exposed in fluorescent light and full spectrum light,on which basis fluorescent TCM group and full spectrum TCM group were added with "Rizhong Yinyang Formulas",respectively.Optical parameters were measured and the influence of different lights on the serum and retinal dopamine(DA) levels as well as the retinal histopathological tissues was observed.Results:The spectrum of fluorescent light mainly focused at 420-490 nm with the peak value of wavelength near 450 nm,whereas that of full spectrum was wider(400-800 nm) with the peak value near 600 nm.After 4 and 12 weeks,fluorescent group was evidently lower in serum and retinal DA levels(P<0.01).and there was no significant difference among full spectrum group,fluorescent TCM group and full spectrum TCM group(P>0.05).Histopathological observation showed that there was significant difference in pigment epithelium layer,photoreceptor and nerve fiber layer between fluorescent group and control group,but the difference among the test groups was not significant.Conclusions:Fluorescent light has certain influence on retinal histological construction and visual performance.However,TCM intervention may have some degree of protective function on retina.

人工光环境对雌性豚鼠生殖内分泌的影响及日钟阴阳方的干预作用

目的研究人工光环境对雌性豚鼠生殖内分泌的影响及日钟阴阳方对雌性豚鼠下丘脑中吻素Kisspeptin及吻素受体(KISS1R)的作用机制。方法将3月龄雌性豚鼠按体重的大小从1到36编号,获取随机数字,按余数分为6组,分别为自然光组、自然光食疗组、LED灯组、LED灯食疗组、荧光灯组和荧光灯食疗组,每组6只。用日钟阴阳方对自然光食疗组、LED灯食疗组、荧光灯食疗组进行干预12周。饲养12周后,于动情前期取材,HE染色,电镜下观察卵巢形态;ELISA测定豚鼠血清中雌二醇(E2)、促黄体生成素(LH)、促卵泡生成素(FSH)、睾酮(T)、抗苗勒氏管激素(AMH)、泌乳素(PRL)水平;Western Blot测定豚鼠卵巢内雄激素受体(AR)、促黄体生成素受体(LHR)、促卵泡生成素受体(FSHR)及下丘脑中KISS1R的蛋白含量及逆转录聚合酶链式反应法(RT-PCR)测定豚鼠卵巢内AR、LHR、FSHR mRNA及下丘脑中KISS1R mRNA表达水平。结果(1)自然光照组及自然光食疗组可见结构完整的卵母细胞,未见明显病理变化;荧光灯组及LED灯组卵巢卵母细胞消失,见大量炎性细胞浸润;荧光灯食疗组及LED灯食疗组卵巢可见卵母细胞,但卵母细胞周围粒细胞排列疏松,间隙增大。(2)与自然光照组比较,自然光食疗组血清E2、LH、FSH、T、AMH、PRL含量下降,下丘脑内KISS1R及卵巢内LHR、FSHR、AR蛋白含量下降(P<0.05);与荧光灯组比较,荧光灯食疗组血清血清E2、LH、FSH、T、AMH、PRL含量下降,下丘脑内KISS1R及卵巢内LHR、FSHR、AR蛋白含量下降(P<0.05);与LED灯组比较,LED灯食疗组血清E2、LH、FSH、T、AMH、PRL含量均下降,下丘脑内KISS1R及卵巢内LHR、FSHR、AR蛋白含量下降(P<0.05);与自然光照组比较,荧光灯、LED灯组血清E2、LH、FSH、T、AMH、PRL含量上升,下丘脑内KISS1R及卵巢内LHR、FSHR、AR蛋白含量上升(P<0.05);与自然光食疗组比较,荧光灯食疗、LED灯食疗组血清E2、LH、FSH、T、AMH、PRL含量上升,下丘脑内KISS1R及卵巢内LHR、FSHR、AR蛋白含量上升(P<0.05)。结论人工光环境的改变会对豚鼠生殖内分泌造成影响,日钟阴阳方可有效改善豚鼠生殖内分泌紊乱的结局。其可能机制与通过调控豚鼠下丘脑内KISS1R的表达,影响Kisspeptin的分泌有关。