乐复能对LPS介导的健康人外周血单核细胞分泌TNF-α的影响及其机制

目的:探讨乐复能在体外对LPS介导的健康人外周血单核细胞分泌TNF-α及NF-κB mRNA表达的影响,以期为乐复能治疗克罗恩病等免疫性疾病提供理论依据。方法:分离30例健康人外周血单核细胞并进行体外培养,分别按以下5种方法 (5组)进行体外实验:A组为空白对照组;B组为单纯LPS刺激组;C组为LPS与乐复能同时加入组;D组为先加入LPS刺激,后加入乐复能组;E组为先加入乐复能,后加入LPS刺激组。干预后用ELISA法检测培养液内TNF-α浓度,然后采用RT-PCR方法检测单核细胞内NF-κB mRNA表达情况。结果:基础状态下,体外培养的健康人单核细胞分泌少量TNF-α[(470.23±35.24)pg/mL)],加入LPS刺激后,TNF-α的分泌明显增加[(1446.76±72.36)pg/mL)],在LPS刺激后再加入乐复能,TNF-α的分泌明显减少[(1446.76±72.36)pg/mL vs(946.46±46.12)pg/mL,P<0.01],下降约29.7%。而乐复能在LPS刺激前或与LPS同时加入培养细胞内时,对TNF-α分泌无影响[(1446.76±72.36)pg/mL vs(1275.62±87.75)pg/mL,P>0.05;(1446.76±72.36)pg/mL vs(1383.62±86.96)pg/mL,P>0.05]。乐复能明显下调经LPS诱导的单核细胞内NF-κB mRNA表达(0.2829±0.0365 vs 0.4994±0.0604,P<0.01),而对于未提前接受LPS刺激的单核细胞,乐复能对其NF-κBmRNA表达无影响(0.4716±0.0616vs0.4994±0.0604,P>0.05;0.4767±0.0600vs 0.4994±0.0604,P>0.05)。结论:乐复能在体外能抑制LPS介导的健康人外周血单核细胞分泌TNF-α,具有调节单核细胞免疫功能的作用,其抑制TNF-α分泌功能可能与其下调单核细胞内NF-κB表达有关。

基于NIOS处理器的面阵CCD采集系统设计

设计了一种基于Altera的SOPC Builder的软核处理器NIOSII实现面阵CCD采集的系统;该系统采用集成了时序发生器(LR38617)、垂直驱动放大(LR36685)、模拟前端(IR3Y48A1)等功能的集成芯片模块LR38642驱动RJ21P3AH0PT面阵CCD,经LR38642内部的高速A／D转换后的各像素数字信号在NIOSII处理器的控制下,通过自定义采集IP核以及Avalon总线传输到片外SDRAM存储区;经实验论证,该系统结构紧凑,可控性强,可用于高性能数码相机和实时图像采集与处理等场合。

Incompatible Nodulation of <i>Bradyrhizobium elkanii</i>Strains BLY3-8 and BLY6-1 with <i>Rj</i>3 Gene-Harboring Soybean Cultivars

Bradyrhizobia are known symbiotic partners of soybean. However, some soybean cultivars restrict nodulation by some Bradyrhizobium bacterial strains. These restrictions are related to compatibility between the Rj genes of soybean cultivars and nodulation types of inoculated bacteria. The objective of this study was to determine nodulation incompatibility of Type B strains with Rj3 soybean cultivars. Newly isolated B. elkanii strains BLY3-8 and BLY6-1 from Myanmar and specific strain Bradyrhizobium elkanii USDA33, which are incompatible with Rj3 soybean cultivars, and B. japonicum USDA110 were used as inoculants to check compatibility or incompatibility with Rj3 soybean cultivars. Nitrogen fixation activity was measured by the acetylene reduction method. Ethylene concentration (reduction of acetylene) was determined by flame ionization gas chromatography. According to the inoculation test results, USDA110 was compatible with all soybean cultivars because it formed effective nodules (Figure S1 in Appendix) and possessed nitrogenase activity. Similarly, B. elkanii strains BLY3-8, BLY6-1, and USDA33 were highly compatible with non-Rj and Rj4-gene harboring soybean cultivars because they had the ability to form functional nodules and possessed nitrogenase activity. Inversely, BLY3-8, BLY6-1, and USDA33 were incompatible with Rj3 soybean cultivars because they produced ineffective nodules. Consequently, the ratio of ineffective nodule number to total nodule number was >0.5. Therefore, nodule formation by the newly isolated B. elkanii strains BLY3-8 and BLY6-1 was restricted by the Rj3 soybean cultivars potentially making them useful as specific strains to detect the Rj3 gene in soybean cultivars.

通塞脉方取舍怀牛膝对大鼠缺血性脑损伤的影响

目的探讨通塞脉方取舍怀牛膝对缺血性脑损伤模型大鼠的影响。方法雄性SD大鼠随机分为假手术组、模型组、尼莫地平(32.4 mg/kg)组、通塞脉全方(生药31.74 g/kg)组、去牛膝通塞脉方(生药27.77 g/kg)组。除假手术组与模型组外,其他给药组大鼠连续ig给药3 d,每天1次。给药后除假手术组外,其他组大鼠制备缺血性脑卒中模型,造模后24 h取血及大脑组织,脑组织以TTC染色,观察梗死率;HE染色观察病理学变化;测定血清中高迁移率组蛋白1(HMGB1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的量;免疫组化法观察缺血组织中核因子-κB(NF-κB)的表达。结果模型组大鼠HMGB1、TNF-α、IL-1β的量及NF-κB的表达均明显升高(P<0.05),通塞脉全方及通塞脉去牛膝方均可降低脑缺血大鼠HMGB1、TNF-α、IL-1β的量,抑制NF-κB的表达(P<0.05),其中去牛膝通塞脉方降低IL-1β的作用最强。结论通塞脉全方及去牛膝通塞脉方均可通过抑制HMGB1相关的NF-κB信号通路的激活,发挥抑制炎症反应、减轻脑水肿、保护神经细胞的作用;去除牛膝的通塞脉方不会降低对脑缺血疗效,在抑制炎症因子IL-1β的分泌方面优于通塞脉方全方。