Robinia pseudoacacia ‘Idaho＇ （Robinia x ambigua ‘Idahoensis＇, R. pseudoacacia x R. viscosa） modified by a mtLD gene went through five lines and had characteristics of drought tolerance. Three stages of their mic...Robinia pseudoacacia ‘Idaho＇ （Robinia x ambigua ‘Idahoensis＇, R. pseudoacacia x R. viscosa） modified by a mtLD gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by pre- vious investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory. For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS basal medium with 0.4 mg.U1 IBA and 0.1 mg＇U1 NAA as supplements and were solidified with 0.5% agar; the highest survival rate was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.展开更多
The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima （Vahl.） Fern was con- structed into the higher plant expression vector pBin438 and transformed into Robinia pseudoacacia ‘I...The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima （Vahl.） Fern was con- structed into the higher plant expression vector pBin438 and transformed into Robinia pseudoacacia ‘Idaho＇ by Agrobacterium tu- mefaciens GV3101. Callus was screened with G418. Morphogenesis of shoots and roots of Idaho locust transformed genes was car- ried out on antibiotic media. The transformed plants were verified by PCR and Southern blotting tests that the FpDREB2A gene had been inserted into the genome DNA of Idaho locust.展开更多
Based on the plant regeneration system, a GUS gene transformation system to Idaho locust （Robinia pseudoacacia ‘Idaho＇） mediated by Agrobacterium tumefaciens was established. The successful transformation was conf...Based on the plant regeneration system, a GUS gene transformation system to Idaho locust （Robinia pseudoacacia ‘Idaho＇） mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots fi＇om the infected leaves in the presence of hygromysin; by histochemical X-gluc assays of 15-glucuronidase （GUS） and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% （5 out of 86 plants）. With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southern blot- ting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R. pseudoacacia ＇Idaho＇ mediated with A. tumefaciens.展开更多
Idaho locust （Robinia pseudoacacia ‘Idaho＇） is an exotic multi-purpose tree used in landscaping, soil and water conserva- tion, fodder sources and others. To improve its drought tolerance for reclaiming arid land,...Idaho locust （Robinia pseudoacacia ‘Idaho＇） is an exotic multi-purpose tree used in landscaping, soil and water conserva- tion, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions. Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride （TTC） reductants in roots and soil water content （SWC）. The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees.展开更多
Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requi...Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requirements for reclamation of arid or alkalid lands and vegetation restoration. For this paper, we studied the effects of auxin and cytokine on Idaho locust in vitro regeneration and the establishment of gene transformation systems for plants mediated by Agrobacterium tumefaciens. Results showed that the ratios of cytokinin and auxin were the major factors affecting adventitious bud differentiation on a MS medium; the concentration of 0.5 mg·L^-16-BA benefitted callus proliferation and 0.25 mg·L^-1 IBA promoted shoot rooting; however, a higher IBA concentration will inhibit rooting. The most effective antitoxin for screening transgenic Idaho locust shoots was G418 and the most sensitive concentration of it was 8 mg·L^-1.展开更多
文摘Robinia pseudoacacia ‘Idaho＇ （Robinia x ambigua ‘Idahoensis＇, R. pseudoacacia x R. viscosa） modified by a mtLD gene went through five lines and had characteristics of drought tolerance. Three stages of their micropropagation had been studied by pre- vious investigators. The other two stages, in vitro shoot rooting and plantlet acclimatization, still remained unsolved in the laboratory. For this paper, we studied the later two stages based on the previous achievements. Results showed that the highest rooting rate of Idaho locust was 98.4% when the in vitro shoots, over 2.5 cm in height and 0.08 cm in diameter, were placed on a half strength MS basal medium with 0.4 mg.U1 IBA and 0.1 mg＇U1 NAA as supplements and were solidified with 0.5% agar; the highest survival rate was 98.3% when the rooted plantlets were potted in vermiculite. All the stages for micropropagation of the Idaho locust, modified by the mtl-D gene, were assembled completely. The tissue culture plants grow well in the field.
文摘The transcription factor gene FpDREB2A of Fraxinus pennsylvanica Marsh var. subintegerrima （Vahl.） Fern was con- structed into the higher plant expression vector pBin438 and transformed into Robinia pseudoacacia ‘Idaho＇ by Agrobacterium tu- mefaciens GV3101. Callus was screened with G418. Morphogenesis of shoots and roots of Idaho locust transformed genes was car- ried out on antibiotic media. The transformed plants were verified by PCR and Southern blotting tests that the FpDREB2A gene had been inserted into the genome DNA of Idaho locust.
文摘Based on the plant regeneration system, a GUS gene transformation system to Idaho locust （Robinia pseudoacacia ‘Idaho＇） mediated by Agrobacterium tumefaciens was established. The successful transformation was confirmed by regenerating the shoots fi＇om the infected leaves in the presence of hygromysin; by histochemical X-gluc assays of 15-glucuronidase （GUS） and by PCR and PCR-Southern blotting analysis. The ratio of positive transgenic plants is 5.8% （5 out of 86 plants）. With this system, the target gene DREB was introduced into the leaves of Idaho locust. The transgenic plants regenerated, which was verified by PCR-Southern blot- ting. It is suggested that the transformation system could be a new, simple, reliable and practical route to gene transformation of R. pseudoacacia ＇Idaho＇ mediated with A. tumefaciens.
文摘Idaho locust （Robinia pseudoacacia ‘Idaho＇） is an exotic multi-purpose tree used in landscaping, soil and water conserva- tion, fodder sources and others. To improve its drought tolerance for reclaiming arid land, five lines of transformed mtl-D gene, as osmotic regulator in plant cells, have been selected and managed to determine their drought tolerance under experimental conditions. Qualitative and quantitative variables of transformed plants were studied. The critical value of drought tolerance was determined by detecting the 2,3,5-triphenyl tetrazolium chloride （TTC） reductants in roots and soil water content （SWC）. The critical value for drought tolerance was SWC 6% while for the control plants the critical SWC was 8%; a moderate level of SWC is 13% and the highest SWC for plant endurance was 18%. The method proved to be reliable and sensitive in the evaluation of drought tolerance for forest trees.
文摘Robinia pseudoacacia ‘Idaho＇ is one of several multi-purpose trees used in ornamental, soil and water conservation, fodder and nectar sources. Plant abiotic stress tolerance transformed by genes could meet the requirements for reclamation of arid or alkalid lands and vegetation restoration. For this paper, we studied the effects of auxin and cytokine on Idaho locust in vitro regeneration and the establishment of gene transformation systems for plants mediated by Agrobacterium tumefaciens. Results showed that the ratios of cytokinin and auxin were the major factors affecting adventitious bud differentiation on a MS medium; the concentration of 0.5 mg·L^-16-BA benefitted callus proliferation and 0.25 mg·L^-1 IBA promoted shoot rooting; however, a higher IBA concentration will inhibit rooting. The most effective antitoxin for screening transgenic Idaho locust shoots was G418 and the most sensitive concentration of it was 8 mg·L^-1.
