目的:观察不同浓度脂多糖(LPS)对人脐静脉内皮细胞(HUVECs)Robo4受体表达的作用。方法:体外培养HUVECs,分正常对照组和2个浓度LPS组(正常对照组基础上分别加入终浓度为10μg/m L和100μg/m L LPS),每组重复实验6次。免疫荧光染色及Weste...目的:观察不同浓度脂多糖(LPS)对人脐静脉内皮细胞(HUVECs)Robo4受体表达的作用。方法:体外培养HUVECs,分正常对照组和2个浓度LPS组(正常对照组基础上分别加入终浓度为10μg/m L和100μg/m L LPS),每组重复实验6次。免疫荧光染色及Western blot检测各组细胞Robo4蛋白表达,RT-PCR检测Robo4 m RNA表达。结果:高剂量LPS组HUVECs Robo4蛋白与m RNA表达分别为(0.49±0.08)和(0.23±0.08),较正常对照组(1.35±0.15)和(0.97±0.17)明显下降(P<0.05);低剂量LPS组Robo4蛋白与m RNA表达分别为(0.13±0.13)和(0.94±0.14),较正常对照组均有下降,但差异无统计学意义(P>0.05)。结论:高剂量100μg/m L LPS可下调HUVECs Robo4的表达。展开更多
近年来多发伤的发生率逐年提高,严重程度逐步加剧,伤情也日趋复杂。创伤后脓毒血症及多器官功能障碍综合征（multiple organ dysfunction syndrome,MODS）的发生率和死亡率也随之升高[1-2],我国每年因创伤致死的人数上升至人口死因的第...近年来多发伤的发生率逐年提高,严重程度逐步加剧,伤情也日趋复杂。创伤后脓毒血症及多器官功能障碍综合征（multiple organ dysfunction syndrome,MODS）的发生率和死亡率也随之升高[1-2],我国每年因创伤致死的人数上升至人口死因的第4-5位。内皮祖细胞（endothelial progenitor cell,EPC）是1997年由Asahara等[3]首先从外周血液单个核细胞中分离出的一种祖细胞群,具有增殖、迁徙和分化为成熟内皮细胞的能力。展开更多
Objective:To study the correlation of serum ROBO4 and soluble B7-H4 (sB7-H4) contents with the pathological features of tumor in patients with ovarian cancer.Methods:The patients with ovarian cancer and the patients w...Objective:To study the correlation of serum ROBO4 and soluble B7-H4 (sB7-H4) contents with the pathological features of tumor in patients with ovarian cancer.Methods:The patients with ovarian cancer and the patients with ovarian benign tumor who underwent surgical resection in our hospital between March 2015 and August 2017 were selected, and the contents of ROBO4 and sB7-H4 in serum as well as the expression of ROBO4, B7-H4 and oncogenes in the lesion tissues were determined.Results: ROBO4 content in serum of patients with ovarian cancer was significantly lower than that of patients with ovarian benign tumor whereas sB7-H4 content was significantly higher than that of patients with ovarian benign tumor, ROBO4 mRNA expression in ovarian cancer tissues was significantly lower than that in ovarian benign tumor tissues whereas B7-H4 mRNA expression was significantly higher than that in ovarian benign tumor tissues, and ROBO4 and sB7-H4 contents in serum of patients with ovarian cancer were positively correlated with ROBO4 and B7-H4 mRNA expression in ovarian cancer tissues;the higher the FIGO stage and pathological grade of the tumor is, the lower the ROBO4 content and the higher the sB7-H4 content in serum of patients with ovarian cancer will be;β-catenin, CyclinD1 and MMP9 mRNA expression in ovarian cancer tissues were significantly higher than those in ovarian benign tumor tissues, negatively correlated with ROBO4 and positively correlated with sB7-H4 whereas PTEN and E-cadherin mRNA expression were significantly lower than those in ovarian benign tumor tissues, positively correlated with ROBO4 and negatively correlated with sB7-H4.Conclusion: The changes of ROBO4 and sB7-H4 contents in serum of patients with ovarian cancer are closely related to the changes of clinicopathologic stage and the abnormal expression of oncogenes.展开更多
文摘目的:观察不同浓度脂多糖(LPS)对人脐静脉内皮细胞(HUVECs)Robo4受体表达的作用。方法:体外培养HUVECs,分正常对照组和2个浓度LPS组(正常对照组基础上分别加入终浓度为10μg/m L和100μg/m L LPS),每组重复实验6次。免疫荧光染色及Western blot检测各组细胞Robo4蛋白表达,RT-PCR检测Robo4 m RNA表达。结果:高剂量LPS组HUVECs Robo4蛋白与m RNA表达分别为(0.49±0.08)和(0.23±0.08),较正常对照组(1.35±0.15)和(0.97±0.17)明显下降(P<0.05);低剂量LPS组Robo4蛋白与m RNA表达分别为(0.13±0.13)和(0.94±0.14),较正常对照组均有下降,但差异无统计学意义(P>0.05)。结论:高剂量100μg/m L LPS可下调HUVECs Robo4的表达。
文摘目的探讨Slit2/Robo4信号通路在神经元迁移中的活力与凋亡影响,为阐明脑缺血后神经血管新生的机制提供理论和实验依据。方法选择成年雄性远交群（SD）大鼠,建立缺血性脑卒中动物模型,采用Western blot方法测定梗死侧与非梗死侧Slit2和Robo4的表达。取原代培养10-14d的神经元种六孔板,分为加药组H2O2（100μmol/L）、对照组、H2O2（100μmol/L）＋Slit2（3μg/mL）组,行MTT方法检测细胞活力与双染流式细胞术检测细胞凋亡情况。结果 Western blot结果显示,梗死侧Slit2和Robo4表达均较非梗死侧明显升高,差异有统计学意义（P〈0.05）。100μmol/L H2O2处理原代培养神经元24h会导致Slit2和Robo4表达升高（P〈0.05）。MTT结果示,H2O2、H2O2＋Slit2组细胞活力都有明显下降,其中,H2O2＋Slit2组的细胞存活率明显高于单纯H2O2组（P〈0.05）。流式细胞学显示,H2O2＋Slit2组的细胞凋亡率明显低于H2O2组,差异有统计学意义（P〈0.05）。结论缺血性脑卒中有内源性Slit2/Robo4信号通路的激活,而外源性Slit2可以促进氧化应激时神经元的存活能力,抑制其凋亡作用,从而起到神经保护作用。
文摘Objective:To study the correlation of serum ROBO4 and soluble B7-H4 (sB7-H4) contents with the pathological features of tumor in patients with ovarian cancer.Methods:The patients with ovarian cancer and the patients with ovarian benign tumor who underwent surgical resection in our hospital between March 2015 and August 2017 were selected, and the contents of ROBO4 and sB7-H4 in serum as well as the expression of ROBO4, B7-H4 and oncogenes in the lesion tissues were determined.Results: ROBO4 content in serum of patients with ovarian cancer was significantly lower than that of patients with ovarian benign tumor whereas sB7-H4 content was significantly higher than that of patients with ovarian benign tumor, ROBO4 mRNA expression in ovarian cancer tissues was significantly lower than that in ovarian benign tumor tissues whereas B7-H4 mRNA expression was significantly higher than that in ovarian benign tumor tissues, and ROBO4 and sB7-H4 contents in serum of patients with ovarian cancer were positively correlated with ROBO4 and B7-H4 mRNA expression in ovarian cancer tissues;the higher the FIGO stage and pathological grade of the tumor is, the lower the ROBO4 content and the higher the sB7-H4 content in serum of patients with ovarian cancer will be;β-catenin, CyclinD1 and MMP9 mRNA expression in ovarian cancer tissues were significantly higher than those in ovarian benign tumor tissues, negatively correlated with ROBO4 and positively correlated with sB7-H4 whereas PTEN and E-cadherin mRNA expression were significantly lower than those in ovarian benign tumor tissues, positively correlated with ROBO4 and negatively correlated with sB7-H4.Conclusion: The changes of ROBO4 and sB7-H4 contents in serum of patients with ovarian cancer are closely related to the changes of clinicopathologic stage and the abnormal expression of oncogenes.
