A multi-criteria decision-making approach for comparing sample preservation and DNA extraction methods from swine feces

Molecular microbiological methods, such as competetive PCR, real-time PCR, denaturing gradient gel electrophoresis (DGGE) and large-scale parallel-pyrosequencing, require the extraction of sufficient quantity of high quality DNA from microbiologically and chemically complex matrices. Due to difficulties in the field to standardize/select the optimum DNA preservation-extraction methods in view of laboratories differences, this article attempts to present a straight-forward mathematical framework for comparing some of the most commonly used methods. To this end, as a case study, the problem of selecting an optimum sample preservation-DNA extraction strategy for obtaining total bacterial DNA from swine feces was considered. Two sample preservation methods (liquid nitrogen and RNAlater?) and seven extraction techniques were paired and compared under six quantitative DNA analysis criteria: yield of extraction, purity of extracted DNA (A260/280 and A 260/230 ratios), duration of extraction, degradation degree of DNA, and cost. From a practical point of view, it is unlikely that a single sample preservation-DNA extraction strategy can be optimum for all selected criteria. Hence, a systematic multi-criteria decision-making (MCDM) approach was used to compare the methods. As a result, the ZR Fecal DNA MiniPrepTM DNA extraction kit for samples preserved either with liquid nitrogen or RNAlater? were identified as potential optimum solutions for obtaining total bacterial DNA from swine feces. Considering the need for practicality for in situ applications, we would recommend liquid nitrogen as sample preservation method, along with the ZR Fecal DNA MiniPrepTM kit. Total bacterial DNA obtained by this strategy can be suitable for downstream PCR-based DNA analyses of swine feces.

Control Method of Effect of Robust Optimization in Multi-Player Multi-Objective Decision-Making

In the real situations of supply chain, there are different parts such as facilities, logistics warehouses and retail stores and they handle common kinds of products. In this research, these situations are focused on as the background of this research. They deal with the common quantities of their products, but due to their different environments, the optimal production quantity of one part can be unacceptable to another part and it may suffer a heavy loss. To avoid that kind of unacceptable situations, the common production quantities should be acceptable to all parts in one supply chain. Therefore, the motivation of this research is the necessity of the method to find the production quantities that make all decision makers acceptable is needed. However, it is difficult to find the production quantities that make all decision makers acceptable. Moreover, their acceptable ranges do not always have common ranges. In the decision making of car design, there are similar situations to this type of decision making. The performance of a car consists of purposes such as fuel efficiency, size and so on. Improving one purpose makes another worse and the relationship between these purposes is tradeoff. In these cases, Suriawase process is applied. This process consists of negotiations and reviews of the requirements of the purposes. In the step of negotiations, the requirements of the purposes are share among all decision makers and the solution that makes them as satisfied as possible. In the step of reviews of the requirements, they are reviewed based on the result of the negotiation if the result is unacceptable to some of decision makers. Therefore, through the iterations of the two steps, the solution that makes all decision makers satisfied is obtained. However, in the previous research, the effects that one decision maker reviews requirements in Suriawase process are quantified, but the mathematical model to modify the ranges of production quantities of all decision makers simultaneously is not shown. Therefore, in this research, based on Suriawase process, the mathematical model of multi-player multi-objective decision making is proposed. The mathematical model of multi-player multi-objective decision making by using linear physical programming (LPP) and robust optimization (RO) in the previous research is the basis of the methods of this research. LPP is one of the multi-objective optimization methods and RO is used to make the balance of the preference levels among decision makers. In LPP, the preference ranges of all objective functions are needed, so as the hypothesis of this research. In the research referred in this research, the method to control the effect of RO is not shown. If the effect of RO is too big, the average of the preference level becomes worse. The purpose of this research is to reproduce the mathematical model of multi-player multi-objective decision making based on Suriawase process and propose the method to control the effect of RO. In the proposed model, a set of the solutions of the negotiation problem is obtained and it is proved by the result of the numerical experiment. Therefore, the conclusion that the proposed model is available to obtain a set of the solutions of the negotiation problems in supply chain.

Multi-attribute decision-making based on subjective and objective integrated eigenvector method

An integrated approach is proposed to investigate the fuzzy multi-attribute decision-making （MADM） problems, where subjective preferences are expressed by a pairwise comparison matrix on the relative weights of attributes and objective information is expressed by a decision matrix. An eigenvector method integrated the subjective fuzzy preference matrix and objective information is proposed. Two linear programming models based on subjective and objective information are introduced to assess the relative importance weights of attributes in an MADM problem. The simple additive weighting method is utilized to aggregate the decision information, and then all the alternatives are ranked. Finally, a numerical example is given to show the feasibility and effectiveness of the method. The result shows that it is easier than other methods of integrating subjective and objective information.

Robustness Study and Superior Method Development and Validation for Analytical Assay Method of Atropine Sulfate in Pharmaceutical Ophthalmic Solution

Background: The robustness is a measurement of an analytical chemical method and its ability to contain unaffected by little with deliberate variation of analytical chemical method parameters. The analytical chemical method variation parameters are based on pH variability of buffer solution of mobile phase, organic ratio composition changes, stationary phase (column) manufacture, brand name and lot number variation;flow rate variation and temperature variation of chromatographic system. The analytical chemical method for assay of Atropine Sulfate conducted for robustness evaluation. The typical variation considered for mobile phase organic ratio change, change of pH, change of temperature, change of flow rate, change of column etc. Purpose: The aim of this study is to develop a cost effective, short run time and robust analytical chemical method for the assay quantification of Atropine in Pharmaceutical Ophthalmic Solution. This will help to make analytical decisions quickly for research and development scientists as well as will help with quality control product release for patient consumption. This analytical method will help to meet the market demand through quick quality control test of Atropine Ophthalmic Solution and it is very easy for maintaining (GDP) good documentation practices within the shortest period of time. Method: HPLC method has been selected for developing superior method to Compendial method. Both the compendial HPLC method and developed HPLC method was run into the same HPLC system to prove the superiority of developed method. Sensitivity, precision, reproducibility, accuracy parameters were considered for superiority of method. Mobile phase ratio change, pH of buffer solution, change of stationary phase temperature, change of flow rate and change of column were taken into consideration for robustness study of the developed method. Results: The limit of quantitation (LOQ) of developed method was much low than the compendial method. The % RSD for the six sample assay of developed method was 0.4% where the % RSD of the compendial method was 1.2%. The reproducibility between two analysts was 100.4% for developed method on the contrary the compendial method was 98.4%.

A robust triaxial localization method of AE source using refraction path

Acoustic emission(AE)localization algorithms based on homogeneous media or single-velocity are less accurate when applied to the triaxial localization experiments.To the end,a robust triaxial localization method of AE source using refraction path is proposed.Firstly,the control equation of the refraction path is established according to the sensor coordinates and arrival times.Secondly,considering the influence of time-difference-of-arrival(TDOA)errors,the residual of the governing equation is calculated to estimate the equation weight.Thirdly,the refraction points in different directions are solved using Snell’s law and orthogonal constraints.Finally,the source coordinates are iteratively solved by weighted correction terms.The feasibility and accuracy of the proposed method are verified by pencil-lead breaking experiments.The simulation results show that the new method is almost unaffected by the refraction ratio,and always holds more stable and accurate positioning performance than the traditional method under different ratios and scales of TDOA outliers.

Dynamic programming methodology for multi-criteria group decision-making under ordinal preferences

A method of minimizing rankings inconsistency is proposed for a decision-making problem with rankings of alternatives given by multiple decision makers according to multiple criteria. For each criteria, at first, the total inconsistency between the rankings of all alternatives for the group and the ones for every decision maker is defined after the decision maker weights in respect to the criteria are considered. Similarly, the total inconsistency between their final rankings for the group and the ones under every criteria is determined after the criteria weights are taken into account. Then two nonlinear integer programming models minimizing respectively the two total inconsistencies above are developed and then transformed to two dynamic programming models to obtain separately the rankings of all alternatives for the group with respect to each criteria and their final rankings. A supplier selection case illustrated the proposed method, and some discussions on the results verified its effectiveness. This work develops a new measurement of ordinal preferences’ inconsistency in multi-criteria group decision-making （MCGDM） and extends the cook-seiford social selection function to MCGDM considering weights of criteria and decision makers and can obtain unique ranking result.

Determination of the best materials for development and designing product using a multi-criteria decision-making

Purpose–Material selection,driven by wide and often conflicting objectives,is an important,sometimes difficult problem in material engineering.In this context,multi-criteria decision-making(MCDM)methodologies are effective.An approach of MCDM is needed to cater to criteria of material assortment simultaneously.More firms are now concerned about increasing their productivity using mathematical tools.To occupy a gap in the previous literature this research recommends an integrated MCDM and mathematical Bi-objective model for the selection of material.In addition,by using the Technique for Order Preference by Similarity to Ideal Solution(TOPSIS),the inherent ambiguities of decision-makers in paired evaluations are considered in this research.It goes on to construct a mathematical bi-objective model for determining the best item to purchase.Design/methodology/approach–The entropy perspective is implemented in this paper to evaluate the weight parameters,while the TOPSIS technique is used to determine the best and worst intermediate pipe materials for automotive exhaust system.The intermediate pipes are used to join the components of the exhaust systems.The materials usually used to manufacture intermediate pipe are SUS 436LM,SUS 430,SUS 304,SUS 436L,SUH 409 L,SUS 441 L and SUS 439L.These seven materials are evaluated based on tensile strength(TS),hardness(H),elongation(E),yield strength(YS)and cost(C).A hybrid methodology combining entropy-based criteria weighting,with the TOPSIS for alternative ranking,is pursued to identify the optimal design material for an engineered application in this paper.This study aims to help while filling the information gap in selecting the most suitable material for use in the exhaust intermediate pipes.After that,the authors searched for and considered eight materials and evaluated them on the following five criteria:(1)TS,(2)YS,(3)H,(4)E and(5)C.The first two criteria have been chosen because they can have a lot of influence on the behavior of the exhaust intermediate pipes,on their performance and on the cost.In this structure,the weights of the criteria are calculated objectively through the entropy method in order to have an unbiased assessment.This essentially measures the quantity of information each criterion contribution,indicating the relative importance of these criteria better.Subsequently,the materials were ranked using the TOPSIS method in terms of their relative performance by measuring each material from an ideal solution to determine the best alternative.The results show that SUS 309,SUS 432L and SUS 436 LM are the first three materials that the exhaust intermediate pipe optimal design should consider.Findings–The material matrix of the decision presented in Table 3 was normalized through Equation 5,as shown in Table 5,and the matrix was multiplied with weighting criteriaß_j.The obtained weighted normalized matrix V_ij is presented in Table 6.However,the ideal,worst and best value was ascertained by employing Equation 7.This study is based on the selection of material for the development of intermediate pipe using MCDM,and it involves four basic stages,i.e.method of translation criteria,screening process,method of ranking and search for methods.The selection was done through the TOPSIS method,and the criteria weight was obtained by the entropy method.The result showed that the top three materials are SUS 309,SUS 432L and SUS 436 LM,respectively.For the future work,it is suggested to select more alternatives and criteria.The comparison can also be done by using different MCDM techniques like and Choice Expressing Reality(ELECTRE),Decision-Making Trial and Evaluation Laboratory(DEMATEL)and Preference Ranking Organization Method for Enrichment Evaluation(PROMETHEE).Originality/value–The results provide important conclusions for material selection in this targeted application,verifying the employment of mutual entropy-TOPSIS methodology for a series of difficult engineering decisions in material engineering concepts that combine superior capacity with better performance as well as cost-efficiency in various engineering design.

Research on Regulation Method of Energy Storage System Based on Multi-Stage Robust Optimization

To address the scheduling problem involving energy storage systems and uncertain energy,we propose a method based on multi-stage robust optimization.This approach aims to regulate the energy storage system by using a multi-stage robust optimal control method,which helps overcome the limitations of traditional methods in terms of time scale.The goal is to effectively utilize the energy storage power station system to address issues caused by unpredictable variations in environmental energy and fluctuating load throughout the day.To achieve this,a mathematical model is constructed to represent uncertain energy sources such as photovoltaic and wind power.The generalized Benders Decomposition method is then employed to solve the multi-stage objective optimization problem.By decomposing the problem into a series of sub-objectives,the system scale is effectively reduced,and the algorithm’s convergence ability is improved.Compared with other algorithms,the multi-stage robust optimization model has better economy and convergence ability and can be used to guide the power dispatching of uncertain energy and energy storage systems.

Behavioral Decision-Making of Key Stakeholders in Public-Private Partnerships:A Hybrid Method and Benefit Distribution Study

Public-private partnerships(PPPs)have been used by governments around the world to procure and construct infrastructural amenities.It relies on private sector expertise and funding to achieve this lofty objective.However,given the uncertainties of project management,transparency,accountability,and expropriation,this phenomenon has gained tremendous attention in recent years due to the important role it plays in curbing infrastructural deficits globally.Interestingly,the reasonable benefit distribution scheme in a PPP project is related to the behavior decisionmaking of the government and social capital,aswell as the performance of the project.In this paper,the government and social capital which are the key stakeholders of PPP projects were selected as the research objects.Based on the fuzzy expected value model and game theory,a hybrid method was adopted in this research taking into account the different risk preferences of both public entities and private parties under the fuzzy demand environment.To alleviate the problem of insufficient utilization of social capital in a PPP project,this paper seeks to grasp the relationship that exists between the benefit distribution of stakeholders,their behavioral decision-making,and project performance,given that they impact the performance of both public entities and private parties,as well as assist in maximizing the overall utility of the project.Furthermore,four game models were constructed in this study,while the expected value and opportunity-constrained programming model for optimal decision-making were derived using alternate perspectives of both centralized decision-making and decentralized decision-making.Afterward,the optimal behavioral decision-making of public entities and private parties in four scenarios was discussed and thereafter compared,which led to an ensuing discussion on the benefit distribution system under centralized decision-making.Lastly,based on an example case,the influence of different confidence levels,price,and fuzzy uncertainties of PPP projects on the equilibrium strategy results of both parties were discussed,giving credence to the effectiveness of the hybrid method.The results indicate that adjusting different confidence levels yields different equilibriumpoints,and therefore signposts that social capital has a fair perception of opportunities,as well as identifies reciprocal preferences.Nevertheless,we find that an increase in the cost coefficient of the government and social capital does not inhibit the effort of both parties.Our results also indicate that a reasonable benefit distribution of PPP projects can assist them in realizing optimum Pareto improvements over time.The results provide us with very useful strategies and recommendations to improve the overall performance of PPP projects in China.

Bi-level model for shared parking decision-making based on parking lot assignment simulation

In order to carry out comprehensive decision-making of multi-class shared parking measures within a region, a bilevel model assisting decision-making is proposed. The upper level selects parkers＇ average satisfaction and the violation rate during peak hours as indices in object function, and sets probability distribution models describing dynamic parking demand of each site, the feasibility of shared parking scenarios and occupancy requirements during peak hours of each parking lot as restrictions. The simulation model in the lower level sets up rules to assign each parker in the random parking demand series to the proper parking lot. An iterative method is proposed to confirm the state of each parking lot at the start of formal simulations. Besides, two patterns linking initialization and formal simulation are presented to acquire multiple solutions. The results of the numerical examples indicate the effectiveness of the model and solution methods.

One-step spraying method to construct superhydrophobic magnesium surface with extraordinary robustness and multi-functions

The robust magnesium surfaces with multi-functions are highly desirable,and the simple and scalable methods to construct such surfaces are urgently indispensable.Herein,we conducted a one-step spraying method to facilely fabricate the robust coating with multi-functions on magnesium alloys.The as-sprayed magnesium alloys surface is superhydrophobic with a static water contact angle(WCA)of 157.0°and a roll-off angle of 6.0°.Such surface has excellent mechanical,chemical and thermal stabilities,even undergoing various physical and chemical damages,including sand impact(10 gmin^(-1),≥20 min),water impact(2 impacts s^(-1),≥180 min),abrasion(1.00 kPa,≥25 cycles),peeling(≥2.15 kPa),high temperature(200°C,≥24 h),strong acidic/salty/basic media(p H=113)and organic-solvent immersion(ethanol and n-hexane,≥24 h),demonstrating brilliant robustness.Notably,the surface displays multi-functions of corrosion protection,anti-fouling and heat insulation,which will undoubtedly promote the much wider applications of magnesium alloys.

Analysis on decision-making model of plan evaluation based on grey relation projection and combination weight algorithm

In military service joint operations, when there are more operational forces, more multifarious materials are consumed, the support is more complex and fuzzy, the deployment of personnel is more rapid, and the support provided by wartime military material support powers can be more effective. When the principles,requirements, influencing factors and goals of military material support forces are deployed in wartime, an evaluation indicator system is established. Thus, a new combined empowerment method based on an analytic hierarchy process（AHP） is developed to calculate the subjective weights, and the rough entropy method is used to calculate the objective weights. Combination weights can be obtained by calculating the weight preference coefficient error, which is determined by combining the cooperative game method and the minimum deviation into objectives. This approach can determine the grey relation projection coefficient and synthesize the measure scheme superiority to finally optimize the deployment plan using the grey relation projection decision-making method. The results show that the method is feasible and effective;it can provide a more scientific and practical decision-making basis for the military material support power deployment in wartime.

Ranking environmental projects model based on multicriteria decision-making and the weight sensitivity analysis

With the fast growth of Chinese economic, more and more capital will be invested in environmental projects. How to select the environmental investment projects （alternatives） for obtaining the best environmental quality and economic benefits is an important problem for the decision makers. The purpose of this paper is to develop a decision-making model to rank a finite number of alternatives with several and sometimes conflicting criteria. A model for ranking the projects of municipal sewage treatment plants is proposed by using exports＇ information and the data of the real projects. And, the ranking result is given based on the PROMETHEE method. Furthermore, by means of the concept of the weight stability intervals （WSI）, the sensitivity of the ranking results to the size of criteria values and the change of weights value of criteria are discussed. The result shows that some criteria, such as “proportion of benefit to project cost”, will influence the ranking result of alternatives very strong while others not. The influence are not only from the value of criterion but also from the changing the weight of criterion. So, some criteria such as “proportion of benefit to project cost” are key critera for ranking the projects. Decision makers must be cautious to them.

A New Method to Solve Robust Data Reconciliation in Nonlinear Process

Data reconciliation is an effective technique for providing accurate and consistent value for chemical process. However, the presence of gross errors can severely bias the reconciled results. Robust estimators can significantly reduce the effect of gross errors and yield less-biased results. In this article, a new method is proposed to solve the robust data reconciliation problem of nonlinear chemical process. By using several technologies including linearization method, penalty function, virtual observation equation, and equivalent weights method, the robust data reconciliation problem can be transformed into least squares estimator problem which leads to the convenience in computation. Simulation results in a nonlinear chemical process demonstrate the efficiency of the proposed method.

Robust design of natural laminar flow supercritical airfoil by multi-objective evolution method

Abstract A transonic, high Reynolds number natural laminar flow airfoil is designed and studied. The γ-θ transition model is combined with the shear stress transport （SST） k-w turbulence model to predict the transition region for a laminar-turbulent boundary layer. The non-uniform free-form deformation （NFFD） method based on the non-uniform rational B-spline （NURBS） basis function is introduced to the airfoil parameterization. The non-dominated sorting genetic algorithm-II （NSGA-II） is used as the search algo- rithm, and the surrogate model based on the Kriging models is introduced to improve the efficiency of the optimization system. The optimization system is set up based on the above technologies, and the robust design about the uncertainty of the Mach number is carried out for NASA0412 airfoil. The optimized airfoil is analyzed and compared with the original airfoil. The results show that natural laminar flow can be achieved on a supercritical airfoil to improve the aerodynamic characteristic of airfoils.

Approach of technical decision-making by element flow analysis and Monte-Carlo simulation of municipal solid waste stream

This paper deals with the procedure and methodology which can be used to select the optimal treatment and disposal technology of municipal solid waste （MSW）, and to provide practical and effective technical support to policy-making, on the basis of study on solid waste management status and development trend in China and abroad. Focusing on various treatment and disposal technologies and processes of MSW, this study established a Monte-Carlo mathematical model of cost minimization for MSW handling subjected to environmental constraints. A new method of element stream （such as C, H, O, N, S） analysis in combination with economic stream analysis of MSW was developed. By following the streams of different treatment processes consisting of various techniques from generation, separation, transfer, transport, treatment, recycling and disposal of the wastes, the element constitution as well as its economic distribution in terms of possibility functions was identified. Every technique step was evaluated economically. The Mont-Carlo method was then conducted for model calibration. Sensitivity analysis was also carried out to identify the most sensitive factors. Model calibration indicated that landfill with power generation of landfill gas was economically the optimal technology at the present stage under the condition of more than 58% of C, H, O, N, S going to landfill. Whether or not to generate electricity was the most sensitive factor. If landfilling cost increases, MSW separation treatment was recommended by screening first followed with incinerating partially and composting partially with residue landfilling. The possibility of incineration model selection as the optimal technology was affected by the city scale. For big cities and metropolitans with large MSW generation, possibility for constructing large-scale incineration facilities increases, whereas, for middle and small cities, the effectiveness of incinerating waste decreases.

ROBUSTNESS OF THE SUBSPACE METHOD FOR BLIND SIGNATURE WAVEFORM ESTIMATION WITH RESPECT TO CHANNEL ORDER IN ASYNCHRONOUS DS-CDMA SYSTEMS

The robustness of the subspace method for blind signature waveform estimation with respect to channel order is analyzed in asynchronous DS-CDMA systems theoretically. Theoretical analysis and simulation results show that the overestimating of the channel order will lead to the degradation of the quality of the estimated signature waveform. So we should adopt the channel order as small as possible.

The Extension Methods and Fecision-making Analysis on the Planting Dtructure of Dlope Garmland in Northern Shaanxi

Based on the resent research about the planting structure of slope farmland in northern Shaanxi,in order to increase the native's income,we use the theory of the extension methods to analyze the planting structure of slope farmland,so that we can get the extensive strategy,and put them into use for planting of slope farmland to make the native's income higher.

Robust airfoil optimization based on improved particle swarm optimization method

A robust airfoil optimization platform is constructed based on the modified particle swarm optimization method （i.e., the second-order oscillating particle swarm method）, which consists of an efficient optimization algorithm, a precise aerodynamic analysis program, a high accuracy surrogate model, and a classical airfoil parametric method. There are two improvements for the modified particle swarm method compared with the standard particle swarm method. First, the particle velocity is represented by the combination of the particle position and the variation of position, which makes the particle swarm algorithm a second-order precision method with respect to the particle po- sition. Second, for the sake of adding diversity to the swarm and enlarging the parameter searching domain to improve the global convergence performance of the algorithm, an oscillating term is introduced to the update formula of the particle velocity. At last, tak- ing two airfoils as examples, the aerodynamic shapes are optimized on this optimization platform. It is shown from the optimization results that the aerodynamic characteristic of the airfoils is greatly improved in a broad design range.