Study on Characteristic Fingerprint of Chemical Constituents in the Pholidota cantonensis Rolfe.

[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe.and its characteristic fingerprint of chemical constituents,in order to identify P.cantonensis.[Methods] Ten batches of P.cantonensis from different habitats in Guangxi and out of Guangxi (Shaoguan of Guangdong Province) were determined.The materials were extracted in 80% methanol ultrasonically for 1.5 h under the material-to-liquid ratio of 2.5 g/80 ml.The HPLC determination was carried out using an Agilent 1260 HPLP instrument equipped with an Agilent5 TC-C18 (2) column at the column temperature of 30 ℃,the wavelength of 280 nm and the injection volume of 10 μl using acetonitrile-0.4% phosphoric acid as the mobile phase and chlorogenic acid as the reference peak,and the detection took 58 min.[Results] The characteristic fingerprint of the chemical constituents of P.cantonensis were obtained,and the three chromatographic peaks of gallic acid,chlorogenic acid and syringaldehyde were compared.[Conclusions] This study can provide reference for the identification of the Zhuang medicine P.cantonensis and provide guarantee for the clinical use of Chinese medicine.

Pharmacognosy Identification of Xiaohuangcao (Dendrobium loddigesi Rolfe)

[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards.

Research Progress of Pharmacognostical Identification and Chemical Composition of Pholidota cantonensis Rolfe.

Research progress of pharmacognostical identification and chemical composition of Pholidota cantonensis Rolfe. was reviewed. This paper will facilitate assurance of the safety of clinical treatment.

阳光建筑——德国建筑师罗尔夫·迪施(Rolf Disch)的建筑观及建筑实践

探讨了德国著名太阳能建筑师罗尔夫.迪施(Rolf Disch)的建筑观,从他对太阳能的利用、建筑材料与建筑结构的选择、各种生态技术的应用等方面对其具代表性的几个建筑作品进行了分析。通过探讨以期对国内绿色建筑设计起到一定参考作用。

迭鞘石斛所含多糖对小鼠免疫细胞影响的研究

[目的]研究迭鞘石斛(Dendrobinm chryseum Rolfe)所含多糖对小鼠脾淋巴细胞增殖以及腹腔巨噬细胞的影响。[方法]分离提取小鼠脾脏淋巴细胞和腹腔巨噬细胞,通过添加和不添加迭鞘石斛所含多糖进行体外培养,以MTT法检测这些免疫细胞增殖的变化情况。[结果]石斛多糖在中、高浓度时可极显著提高小鼠脾淋巴细胞和腹腔巨噬细胞的增值(P﹤0.01),低浓度时没有增强作用。[结论]迭鞘石斛所含石斛多糖在体外对小鼠脾淋巴细胞增殖以及腹腔巨噬细胞的增殖有增强作用,说明对小鼠具有免疫增强作用。

不同培养基对山慈姑种子无菌萌发的影响

[目的]寻找山慈菇（Pleione bulbocodioides（Franch.）Rolfe）种子萌发的最佳培养条件,为山慈菇试管苗的获得提供技术支撑。[方法]人工授粉获得山慈姑种子,利用其未成熟胚进行种子萌发试验,探讨了不同蔗糖浓度、不同活性炭浓度、不同类型的基本培养基、4种单激素（6-BA、KT、NAA、IAA）及各自不同浓度、2种双激素组合及不同浓度等对山慈姑种子萌发率的影响。[结果]诱导山慈姑种子萌发的最适培养基为1/2MS＋1.5 mg/L IAA＋0.05%干酪素＋2%蔗糖＋0.75%活性炭＋0.55%琼脂（pH 5.8）,且添加了IAA的处理萌发率普遍较高,不仅诱导出原始球茎数量多,而且体积大,平均直径达1~1.5 mm。[结论]该试验建立优质、高效的山慈菇种子萌发体系,可为大量试管苗的获得奠定基础。

蔓性千斤拔最佳采收期及根部总黄酮含量提取工艺研究

为研究蔓性千斤拔[Moghania philippinensis(Merr.Et Rolfe)Li.]的最佳采收期及根部总黄酮含量最佳提取工艺,采用紫外分光光度计法和L9(34)正交试验对其进行了优化。结果表明,蔓性千斤拔在次年10月总黄酮含量最高;在乙醇浓度90%、提取时间60 min、提取2次、提取温度100℃时,根部总黄酮得率最高。

Viktor Rolf

视觉震撼Victor Horsting和Rolf Snoeren对于2011春季时装的理念是在男士衬衫上进行各种部件的重复。这对荷兰人展现在T台上的,毫不意外的,是一系列具有戏剧性的衣服,给人们带来精彩的视觉震撼,给时尚界带来新奇欣喜的冲击。

Viktor & Rolf 独特美学

Viktor & Rolf由Viktor Horsting和Rolf Snoeren这两位毕业于著名的Arnhem Academy of the Arts的荷兰设计师于1993年在巴黎创建。这个二人组合一出道便以新奇的创意、浓烈的实验色彩和骨子里的Couture情结让人们领略到主流时尚以外的另类精彩。他们的设计一直游走在艺术和时尚之间,以反传统的剪裁、颠覆性的比例、夸张的细节和前卫概念性的秀场布置而闻名,其带点玩味的设计及独特的时装美学概念一直是时装传媒的焦点。

Viktor & Rolf 终止成衣系列

荷兰设计师组合Viktor Horsting和Rolf Snoeren决定从2015年秋冬季开始终止Viktor＆Rolf成衣系列,从而专注于品牌的高级定制、香水和授权业务。Viktor＆Rolf将不会参与3月初的2015秋冬巴黎时装周,但会发布和出售其最后一个成衣系列——2015秋冬女装系列。Viktor Horsting和Rolf Snoeren在接受WWD采访时解释,成衣产业的快节奏和激烈竞争使他们的创意受限。

Rolf Benz

作为软装家具业领军人物的Rolf Benz＆ Co.KG公司，在德国科隆家具展（2006年1月16日-22日）上推出一系列新家具，他们还就2006年的软装家具趋势作出预测，即传记性，沉静的奢华，未来与过去的相遇。

Two New Triterpenes from the Orchid Pholidota yunnanensis Rolfe

Abstract: Two novel triterpenes (1 and 2) were isolated from the orchid Pholidota yunnanensis Rolfe. Using chemical and spectral analyses (UV, IR, MS,1D-NMR and 2D-NMR), these two triterpenes were established as 25-methylenecyclopholidonyl p-hydroxy-trans-cinnamate (1; named pholidotine A) and 25-methylenecyclopholidonyl p-hydroxy-cis-cinnamate (2; named pholidotine B).

舟曲县独蒜兰分布及居群特征

配合甘肃省兰科植物调查工作,通过对舟曲县7个调查地独蒜兰[Pleione bulbocodioides(Franch.)Rolfe]分布与居群特征调查。结果显示,其中2个调查地有独蒜兰的分布,分布于城关镇三眼峪沟内西北向的石壁上和拱坝河上游南岸距河面5 m的石台上,证实了舟曲县境内有独蒜兰的分布,舟曲是独蒜兰新的分布记录点,在这个区域以西、以北再没有发现独蒜兰的分布,说明舟曲的这一分布区域是独蒜兰分布的最西北边界,对植物分布区系研究有重要意义。

基于GC-MS代谢组学技术2种香型美花石斛代谢物的对比分析

为明确2种美花石斛之间花香味物质的分子基础,本研究采用GC-MS技术对2种香型美花石斛的低分子量挥发性代谢物进行非靶向代谢组学分析,利用多元统计分析,寻找两者间的差异代谢物以及在次生代谢途径上的区别。结果表明:利用GC-MS在2个不同香型美花石斛中共鉴定出80种代谢物;通过PCA与OPLS-DA分析可知淡香型与浓香型美花石斛间的代谢物差异明显;采用OPLS-DA筛选得到2种美花石斛的显著性差异代谢物38种,富集到次生代谢产物生物合成途径、单萜类生物合成途径、新陈代谢途径、α-亚麻酸代谢途径和苯丙烷生物合成途径5个生物合成途径。本研究发现浓香型美花石斛和淡香型美花石斛挥发性代谢物的种类和含量存在较大差异,对芳香石斛兰品种选育有指导作用。

Rolf Wieleba 中国保温行业的“白求恩”

1961年生于德国拉文斯堡的Rolf Wieleba有着金色的头发、高高的鼻梁,戴着一副眼镜,既有渊博的知识又有＂老外＂的幽默。他,既是外保温行业专家,又是太阳能技术专家、建筑节能专家、德国能源署认证的能源顾问。