Effects of Root Growth of Deep and Shallow Rooting Rice Cultivars in Compacted Paddy Soils on Subsequent Rice Growth

Rice is often grown as multiple seasons in one year,alternating between flooded and upland systems.A major constraint,introduced from the flooded system,is a plough pan that may decrease rooting depth and productivity of follow-on upland rice.Roots penetrating the plough pan under flooded rice system can leave a legacy of weaker root growth pathways.Deeper rooting rice cultivars could have a bigger impact,but no direct evidence is available.To explore whether a deep rather than a shallow rooting rice cultivar grown in a flooded cropping cycle benefited deeper root growth of follow-on rice in an upland,reduced tillage cropping cycle,a simulated flooded paddy in greenhouse was planted with deep(Black Gora) and shallow(IR64) rooting cultivars and a plant-free control.Artificial plough pans were made in between the topsoil and subsoil to form different treatments with no plough pan(0.35 MPa),soft plough pan(1.03 MPa) and hard plough pan(1.70 MPa).After harvest of this ‘first season’ rice,the soil was drained and undisturbed to simulate zero-tillage upland and planted rice cultivar BRRI Dhan 28.The overall root length density(RLD),root surface area,the numbers of root tips and branching of BRRI Dhan 28 did not vary between plough pan and no plough pan treatments.Compared with the shallow rooting rice genotype,the deep rooting rice genotype as ‘first season’ crop produced 19% greater RLD,34% greater surface area and 29% more branching of BRRI Dhan 28 in the subsoil.In the topsoil,however,BRRI Dhan 28 had 28% greater RLD,35% greater surface area and 43% more branching for the shallow rather than deep rooting genotype planted in the ‘first season’.The results suggested that rice cultivar selection for a paddy cycle affects root growth of a follow-on rice crop grown under no-till,with benefits to subsoil access from deep rooting cultivars and topsoil proliferation for shallow rooting cultivars.

Nitrogen application levels based on critical nitrogen absorption regulate processing tomatoes productivity, nitrogen uptake, nitrate distributions, and root growth in Xinjiang, China

The unreasonable nitrogen(N)supply and low productivity are the main factors restricting the sustainable development of processing tomatoes.In addition,the mechanism by which the N application strategy affects root growth and nitrate distributions in processing tomatoes remains unclear.In this study,we applied four N application levels to a field(including 0(N0),200(N200),300(N300),and 400(N400)kg/hm^(2))based on the critical N absorption ratio at each growth stage(planting stage to flowering stage:22%;fruit setting stage:24%;red ripening stage:45%;and maturity stage:9%).The results indicated that N300 treatment significantly improved the aboveground dry matter(DM),yield,N uptake,and nitrogen use efficiency(NUE),while N400 treatment increased nitrate nitrogen(NO_(3)^(-)-N)residue in the 20–60 cm soil layer.Temporal variations of total root dry weight(TRDW)and total root length(TRL)showed a single-peak curve.Overall,N300 treatment improved the secondary root parameter of TRDW,while N400 treatment improved the secondary root parameter of TRL.The grey correlation coefficients indicated that root dry weight density(RDWD)in the surface soil(0–20 cm)had the strongest relationship with yield,whereas root length density(RLD)in the middle soil(20–40 cm)had a strong relationship with yield.The path model indicated that N uptake is a crucial factor affecting aboveground DM,TRDW,and yield.The above results indicate that N application levels based on critical N absorption improve the production of processing tomatoes by regulating N uptake and root distribution.Furthermore,the results of this study provide a theoretical basis for precise N management.

Effects of Allelochemicals on Root Growth and Pod Yield in Response to Continuous Cropping Obstacle of Peanut

Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC obstacle.Treatment with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with control.Exogenous application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological characters.The allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut roots.Meanwhile,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble protein.Analysis of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root system.Consequently,allelochemicals significantly decreased the pod yield of peanut compared with control.Our results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.

Effects of Antimony Stress on Root Growth,Antimony Accumulation and Physiological Characteristics of Ramie(Boehmeria nivea(L.) Gaudich.)

[Objectives]This study was conducted to investigate the toxicity of heavy metal antimony(Sb) to ramie(Boehmeria nivea(L.) Gaudich.) and the tolerance response in ramie. [Methods] A pot experiment was conducted to study the effects of Sb stress on root growth and Sb accumulation and transport of the root system of cultivated ramie Zhongzhu No.1, as well as on the physiological characteristics of ramie leaves. [Results] The plant height and root dry weight and volume of Zhongzhu No.1 showed an effect of "promoting at low concentrations and inhibiting at high concentrations" with the increase of Sb concentration, and decreased significantly at the concentration of 4 000 mg/kg, but no obvious toxic growth symptoms were observed. The content of Sb in roots(289.7-508.6 mg/kg) and the root-shoot transfer factor(0.09-0.57) of Zhongzhu No.1 increased with the increase of soil Sb concentration, but the change of Sb bioconcentration factor in roots was opposite, indicating that high concentrations of Sb in soil could promote the absorption of Sb in roots and the transport of Sb to the aboveground part, but the Sb enrichment capacity of roots was relatively reduced with the increase of soil Sb. Sb stress had a certain impact on the physiological characteristics of ramie leaves. With the increase of Sb treatment concentration, MDA, POD and SOD showed a change trend of "first increasing and then decreasing", while CAT gradually increased, indicating that Sb stress caused changes in the physiological characteristics of ramie leaves, thereby affecting plant growth and development. [Conclusions] This study provides a theoretical basis for ecological restoration of ramie in mining areas.

Genetic Analysis of Root Growth in Rice Seedlings Under Different Water Supply Conditions

To understand the genetic background of root growth of rice ( Oryza sativa L.) seedlings under different water supply conditions, quantitative trait loci (QTLs) and epistatic effect on seminal root length, maximum adventitious root length, adventitious root number, total root dry weight and ratio of root to shoot were detected using molecular map including 103 restriction fragment length polymorphism (RFLP) markers and 104 amplified fragment length polymorphism (AFLP) markers mapped on a recombinant inbred line (RIL) population with 150 lines derived from a cross between an lowland rice IR1552 and an upland rice Azucena in both solution culture (lowland condition) and paper culture (upland condition). Six QTLs and twenty-two pairs of epistatic loci for the four parameters were detected. Three QTLs detected for maximum adventitious root length in solution culture (MARLS), total root dry weight in both solution culture and paper culture (TRDWS and TRDWP) accounted for about 20%, 23% and 13% of the total variations, respectively. Only epistatic loci were found for maximum adventitious root length and adventitious root number in paper culture (MARLP and ARNP), and for ratio of root to shoot in both paper and solution culture (R/SP and R/SS), which accounted for about 12%-61% of the total variations in the parameters, respectively. No identical QTL or epistatic loci were found for the parameters in both solution and paper culture. The results indicate that there is a different genetic system responsible to root growth of rice seedlings under lowland and upland conditions and epistasis might be the major genetic basis for MARLP, ARNP, R/SP and R/SS.

Effect of N Fertilizers on Root Growth and Endogenous Hormones in Strawberry

Endogenous hormones play an important role in the growth and development of roots. The objective of this research was to study the effect of four types of N fertilizers on the root growth of strawberry (Fragaria ananassa Duchesne) and the endogenous enzymes of indole-3-acetic acid (IAA), abscisic acid (ABA), and isopentenyl adenosine (iPA) in its roots and leaves using enzyme-linked immunosorbent assay. Application of all types of N fertilizers significantly depressed (P ≤ 0.05) root growth at 20 d after transplanting. Application of organic-inorganic fertilizer (OIF) as basal fertilizer had a significant negative effect (P ≤ 0.05) on root growth. The application of OIF and urea lowered the lateral root frequency in strawberry plants at 60 d (P ≤ 0.05) compared with the application of two organic fertilizers (OFA and OFB) and the control (CK). With the fertilizer treatments, there were the same concentrations of IAA and ABA in both roots and leaves at the initial growth stage (20 d), lower levels of IAA and ABA at the later stage (60 d), and higher iPA levels at all seedling stages as compared to those of CK. Thus, changes in the concentrations of endogenous phytohormones in strawberry plants could be responsible for the morphological changes of roots due to fertilization.

An R2R3-type transcription factor gene AtMYB59 regulates root growth and cell cycle progression in Arabidopsis

MYB proteins play important roles in eukaryotic organisms. In plants, the R1R2R3-type MYB proteins function in cell cycle control. However, whether the R2R3-type MYB protein is also involved in the cell division process remains unknown. Here, we report that an R2R3-type transcription factor gene, AtMYB59, is involved in the regulation of cell cycle progression and root growth. The AtMYB59 protein is localized in the nuclei of onion epidermal cells and has transactivation activity. Expression of AtMYB59 in yeast cells suppresses cell proliferation, and the transfor- mants have more nuclei and higher anenpioid DNA content with longer cells. Mutation in the conserved domain of AtMYB59 abolishes its effects on yeast cell growth. In synchronized Arabidopsis cell suspensions, the AtMYB59 gene is specifically expressed in the S phase during cell cycle progression. Expression and promoter-GUS analysis reveals that the AtMYB59 gene is abundantly expressed in roots. Transgenic plants overexpressing AtMYB59 have shorter roots compared with wild-type plants （Arabidopsis accession Col-0）, and around half of the mitotic cells in root tips are at metaphase. Conversely, the null mutant myb59-1 has longer roots and fewer mitotic cells at metaphase than Col, suggesting that AtMYB59 may inhibit root growth by extending the metaphase of mitotic cells. AtMYB59 regulates many downstream genes, including the CYCB1;1 gene, probably through binding to MYB-responsive elements. These results support a role forAtMYB59 in cell cycle regulation and plant root growth.

The Arabidopsis P450 protein CYP82C2 modulates jasmonateinduced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis

Jasmonic acid （JA） is a fatty acid-derived signaling molecule that regulates a broad range of plant defense responses against herbivores and some microbial pathogens. Molecular genetic studies have established that JA also performs a critical role in several aspects of plant development. Here, we describe the characterization of the Arabidopsis mutantjasmonic acid-hypersensitivel-1 （jah1-1）, which is defective in several aspects of JA responses. Although the mutant exhibits increased sensitivity to JA in root growth inhibition, it shows decreased expression of JA-inducible defense genes and reduced resistance to the necrotrophic fungus Botrytis cinerea. Gene cloning studies indicate that these defects are caused by a mutation in the cytochrome P450 protein CYP82C2. We provide evidence showing that the compromised resistance of thejah1-1 mutant to B. cinerea is accompanied by decreased expression of JA-induced defense genes and reduced accumulation of JA-induced indole glucosinolates （IGs）. Conversely, the enhanced resistance to B. cinerea in CYP82C2-overexpressing plants is accompanied by increased expression of JA-induced defense genes and elevated levels of JA-induced IGs. We demonstrate that CYP82C2 affects JA-induced accumulation of the IG biosynthetic precursor tryptophan （Trp）, but not the JA-induced IAA or pathogen-induced camalexin. Together, our results support a hypothesis that CYP82C2 may act in the metabolism of Trp-derived secondary metabolites under conditions in which JA levels are elevated. Thejah1-1 mutant should thus be important in future studies toward understanding the mechanisms underlying the complexity of JA-mediated differential responses, which are important for plants to adapt their growth to the ever-changing environments.

Root growth and spatio-temporal distribution of three common annual halophytes in a saline desert, northern Xinjiang

Root growth and spatial and temporal distribution in the 0-100 cm soil profiles of three common annual halophytes Salsola subcrassa, Suaeda acuminate and Petrosimonia sibirica distributed in a saline desert in north- ern Xinjiang, China were studied in 2009 and 2010. The results showed that the root systems of the three halo- phytes were of the taproot type, vertically distributed in the 90-cm soil profile, and were deepest in late July. Their taproots reached maximum depth rapidly, early in the growth period, but with rare lateral roots. They were then dug out in an orderly way, from bottom to top, exhibiting vertical development first and then horizontal development. The distribution of specific root length, which reflects the characteristics of the feeder root, was gradually increased from top to bottom, whereas root weight displayed an opposite distribution pattern. The root length distribution of the three halophytes was concentrated （62% to 76%） in the middle soil profile （20-60 cm）, with less distribution in the surface （0-20 cm） and bottom （60-90 cm） soil profiles. The results indicated that the roots of the three annual halophytes grew rapidly into the deeper soil layer after germination, which ensured the plant survival and uptake of water and nutrition, and thus built up a strong tolerance to an arid, high-salt environment.

Effects of Phosphorus Application in Different Soil Layers on Root Growth, Yield, and Water-Use Efficiency of Winter Wheat Grown Under Semi-Arid Conditions

Deep phosphorus application can be a usefull measure to improve crops＇ performance in semi-arid regions, but more knowledge of both its general effects and effects on specific crops is required to optimize treatments. Thus, the aims of this study were to evaluate the effects of phosphorus（P） application at different soil layers on root growth, grain yield, and water-use efficiency（WUE） of winter wheat grown on the semi-arid Loess Plateau of China and to explore the relationship between root distribution and grain yield. The experiment consisted of four P treatments in a randomized complete block design with three replicates and two cultivars： one drought-sensitive（Xiaoyan 22, XY22） and one drought-tolerant（Changhan 58, CH58）. The four P treatments were no P（control, CK）, surface P（SP）, deep P（DP）, and deep-band P application（DBP）. CH58 produced larger and deeper root systems, and had higher grain yields and WUE, under the deep P treatments（DP and DBP） than under SP, clearly showing that deep P placement had beneficial effects on the drought-tolerant cultivar. In contrast, the grain yield and root growth of XY22 did not differ between DP or DBP and SP treatments. Further, root dry weight（RW） and root length（RL） in deep soil layer（30-100 cm） were closely positively correlated with grain yield and WUE of CH58（but not XY22）, highlighting the connections between a well-developed subsoil root system and both high grain yield and WUE for the drought-tolerant cultivar. WUE correlated strongly with grain yield for both cultivars（r=0.94, P〈0.001）. In conclusion, deep application of P fertilizer is a practical and feasible means of increasing grain yield and WUE of rainfed winter wheat in semi-arid regions, by promoting deep root development of drought-tolerant cultivars.

Exogenous strigolactones promote lateral root growth by reducing the endogenous auxin level in rapeseed

Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great effects on the plant growth and seed yield.Here,we treated the seedlings of Zhongshuang 11(ZS11),an elite conventional rapeseed cultivar,with different concentrations of GR24(a synthetic analogue of strigolactones),and found that a low concentration(0.18μmol L–1)of GR24 could significantly increase the lateral root growth,shoot growth,and root/shoot ratio of seedlings.RNA-Seq analysis of lateral roots at 12 h,1 d,4 d,and 7 d after GR24 treatment showed that 2301,4626,1595,and 783 genes were significantly differentially expressed,respectively.Function enrichment analysis revealed that the plant hormone transduction pathway,tryptophan metabolism,and the phenylpropanoid biosynthesis pathway were over-represented.Moreover,transcription factors,including AP2/ERF,AUX/IAA,NAC,MYB,and WRKY,were up-regulated at 1 d after GR24 treatment.Metabolomics profiling further demonstrated that the amounts of various metabolites,such as indole-3-acetic acid(IAA)and cis-zeatin were drastically altered.In particular,the concentrations of endogenous IAA significantly decreased by 52.4 and 75.8%at 12 h and 1 d after GR24 treatment,respectively.Our study indicated that low concentrations of exogenous SLs could promote the lateral root growth of rapeseed through interaction with other phytohormones,which provides useful clues for the effects of SLs on root architecture and crop productivity.

Phytosulfokine-α Promotes Root Growth by Repressing Expression of Pectin Methylesterase Inhibitor (PMEI) Genes in Medicago truncatula

Phytosulfokine-α(PSK-α),a sulfated pentapeptide with the sequence YIYTQ,is encoded by a small precursor gene family in Arabidopsis.PSK-αregulates multiple growth and developmental processes as a novel peptide hormone.Despite its importance,functions of PSK-αin M.truncatula growth remains unknown.In this study,we identified five genes to encode PSK-αprecursors in M.truncatula.All of these precursors possess conserved PSK-αsignature motif.Expression pattern analysis of these MtPSK genes revealed that each gene was expressed in a tissue-specific or ubiquitous pattern and three of them were remarkably expressed in root.Treatment of M.truncatula seedlings with synthetic PSK-αpeptide significantly promoted root elongation.In addition,expression analysis of downstream genes by RNA-seq and qRT-PCR assays suggested that PSK-αsignaling might regulate cell wall structure via PMEI-PME module to promote root cell growth.Taken together,our results shed light on the mechanism by which PSK-αpromotes root growth in M.truncatula,providing a new resource for improvement of root growth in agriculture.

Effect of wood chip application on root growth of oak seedling and weed control in northern Iran

It was hypothesized that wood chips can serve as a mulch to improve the growth of young trees by facilitating the development of their root systems, inhibiting weed germination, and suppressing weed growth. The present study was carried out in Ghorogh Nursery, Golestan Northern Iran, in order to investigate the impact of wood chips applica- tion on root growth of oak （Quercus castaneifolia） seedlings and control of weed. A three centimeter wood chip layer was used on the soil surface as the mulch treatment with bare soil as the control. The number of new roots, the length of exciting roots and the density of weeds were meas- ured after 5 and 12 weeks. Results showed that wood chip application had significant positive effect on the length of excising roots after 5 and 12 weeks compared with control seedlings. Also, weeds were reduced to near zero levels in treated plot. Our findings suggested that wood chip layer on the soil surface in the nursery can conserve soil moisture and prevent nutrient leaching from the rooting zone as well as diminish weed growth which consequently lead to production of high quality seedling.

Effect of EuCl_3 on Hairy Root Growth of Cassia Obtusifolia and Production of Its Active Principle

Treated with extremely low EuCl 3 (0 001, 0 01, 1 0 mg·L -1 ) in MS medium, the hairy root growth of Cassia obtusifolia was inhibited. The inhibition was strongest at the treatment with 0 001 mg·L -1 EuCl 3, and the dry weight of hairy roots was 22% lower than that of control. Treated with rather low EuCl 3 (10 mg·L -1 ) in Murashige and Skoog(MS) medium, the hairy root growth was enhanced, and the dry weight of hairy roots was 27% higher than that of control. In the each treatment with 0 001 to 1 0 mg·L -1 EuCl 3 in MS medium, the total content of six free anthraquinones was lower than that of control. While treated with 10 mg·L -1 EuCl 3, the total content of six free anthraquinones was 26% higher than that of control, 97% higher than the treatment of 1 0 g·L -1 EuCl 3. Treatment with 10 mg·L -1 EuCl 3 in MS medium enhances the growth of hairy root of Cassia obtusifolia and improves the total content and production of six free anthraquinones in them.

Variations in shoot and root growth of three provenances of Faidherbia albida in clay and sand soil

The variation in shoot and root seedling growth traits was compared among three Faidherbia albida （Del）. A. Chev. provenances originating from Kenya （Taveta）, Malawi （Bolero） and Ghana （Bolgatanga）; representing east, south and west Africa, respectively. Bulked seeds from three provenances were grown in two soil types （clay and sand） at the World Agroforestry Centre nursery, Nairobi. Seedlings grown in clay soil had high shoot growth and shoot to root ratios but shorter root length, lower number of nodules and specific root length compared to seedlings in sand soil. Shoot to root ratios reduced with time and this was more pronounced in sand soil. Overall, the Bolero and Taveta provenances had higher growth than the Bolgatanga provenance. This was probably because plants grown in nutrient-rich clay soil invest more in shoot growth as compared to root growth. Longer root growth in sand soil shows a better adaptive mechanism that increases competitive ability and survival in nutrient-poor systems. Variations observed among the seedling variables such us shoot and root growth could be used for early selection for reforestation and agroforestry in predominantly clay and sand soil areas.

Shoot Photosynthesis and Root Growth of Hybrid and Conventional Rice Cultivars as Affected by N and K Levels in the Root Zone

Root box experiments were conducted to evaluate the effects of N and NK levels in the root zone on shoot photosynthesis and root growth of hybrid and cultivar of rice (Oryza sativa L.) on two paddy soils (clayey and silty). The results showed that dry matter yields in the hybrid and the cultivar were considerably increased by NK supply, but the effect was greater for the hybrid. Supply of NK in the root zone significantly increased photosynthetic rate of the lower position leaf and the active green leaf area per plant, in which the effects were much more obvious in the hybrid rice than in the cultivar. High NK supply in the root zone stimulated the root growth, and decreased PH and increased the oxidation zone in the rhizosphere in both entries, but to a greater extent in the hybrid. The results indicated that higher NK levels were needed to maintain higher root activity and shoot photosynthetic capacity in rice, particularly in hybrid rice.

imple Bioassay for PAMP-Triggered Immunity in Rice Seedlings Based on Lateral Root Growth Inhibition

Pathogen-associated molecular pattern(PAMP)-triggered immunity(PTI)is an essential layer of plant disease resistance.Robust bioassays for PTI are pre-required to dissect its molecular mechanism.In this study,we established that lateral root growth inhibition as a simple and robust measurement of PTI in rice seedlings.Specifically,flg22,a well-characterized PAMP from bacterial flagellin,was used to induce PTI in rice seedlings.While flg22 treatment induced PR gene expression and mitogen-activated protein kinase activation in the roots of rice seedlings to support the PTI triggered,this treatment substantially repressed lateral root growth,but it did not alter primary root growth.Moreover,treatments with chitin(i.e.,a fungal PAMP)and oligogalacturonides(i.e.,classical damage-associated molecular pattern)clearly inhibited the lateral root growth,although a priming step involving ulvan was required for the chitin treatment.The bioassay developed was applicable to various rice cultivars and wild species.Thus,lateral root growth inhibition represents a simple and reliable assay for studying PTI in rice plants.

Seminal, adventitious and lateral root growth and physiological responses in rice to upland conditions

Understanding the growth and physiological responses of rice to upland conditions would be helpful for designing treatments to improve the tolerance of rice under a rainfed system. The objective of this study was to investigate the initiation,elongation and membrane stability of seminal, lateral and adventitious roots of upland rice after 9 d upland condition treatment. Compared with control roots under waterlogged conditions, upland water deficiency conditions favor seminal and lateral root growth over adventitious root growth by accelerating seminal root elongation, promoting lateral root initiation and elongation, and reducing the elongation and number of adventitious roots. Enhanced total root number and length resulted in increase of total root dry weight and thereby increasing the root to shoot ratio. Organic compound leakage from seminal root tips and adventitious roots increased progressively to some extent with upland culture duration, while significant increases in seminal root tips were the consequence of loss of membrane integrity caused by the upland condition enhanced growth.

Responses of root growth of Alhagi sparsifolia Shap. (Fabaceae) to different simulated groundwater depths in the southern fringe of the Taklimakan Desert, China

Alhagi sparsifolia Shap. （Fabaceae） is a spiny, perennial herb. The species grows in the salinized, arid regions in North China. This study investigated the response characteristics of the root growth and the dis- tribution of one-year-old A. sparsifolia seedlings to different groundwater depths in controlled plots. The eco- logical adaptability of the root systems of A. sparsifolia seedlings was examined using the artificial digging method. Results showed that： （1） A. sparsifolia seedlings adapted to an increase in groundwater depth mainly through increasing the penetration depth and growth rate of vertical roots. The vertical roots grew rapidly when soil moisture content reached 3%-9%, but slowly when soil moisture content was 13%-20%. The vertical roots stopped growing when soil moisture content reached 30% （the critical soil moisture point）. （2） The morphological plasticity of roots is an important strategy used by A. sparsifolia seedlings to obtain water and adapt to dry soil conditions. When the groundwater table was shallow, horizontal roots quickly expanded and tillering increased in order to compete for light resources, whereas when the groundwater table was deeper, vertical roots developed quickly to exploit space in the deeper soil layers. （3） The decrease in groundwater depth was probably respon- sible for the root distribution in the shallow soil layers. Root biomass and surface area both decreased with soil depth. One strategy of A. sparsifolia seedlings in dealing with the increase in groundwater depth is to increase root biomass in the deep soil layers. The relationship between the root growth/distribution of A. sparsifolia and the depth of groundwater table can be used as guidance for harvesting A. sparsifolia biomass and managing water resources for forage grasses. It is also of ecological significance as it reveals how desert plants adapt to arid environments.

Changes in gas exchange,root growth,and biomass accumulation of Platycladus orientalis seedlings colonized by Serendipita indica

Serendipita indica(formerly known as Piriformospora indica),a root endophytic fungus,exhibits multiple functions in some agricultural,horticultural,and medicinal plant species.We studied colonization of the roots of Platycladus orientalis,a forest tree species,by S.indica to improve the quality of the seedlings in seedbeds and survival rates in sylviculture.At 20 days after inoculation,S.indica colonized the root cortex of P.orientalis seedlings.Root colonization by S.indica significantly increased net CO2 assimilation,light use efficiency,and biomass accumulation by both roots and shoots,whereas it did not affect the biomass allocation between roots and shoots.In addition,the symbiosis significantly increased root total length,surface area,and volume.In view of the two specific traits of S.indica,i.e.,axenic culture and wide colonization in plants,the fungus might be used for improving quality of P.orientalis seedlings and increasing their survival after transplanting.