Mercury （Hg） is one of the most toxic heavy metals to living organisms and its conspicuous effect is the inhibition of root growth. However, little is known about the molecular genetic basis for root growth under ex...Mercury （Hg） is one of the most toxic heavy metals to living organisms and its conspicuous effect is the inhibition of root growth. However, little is known about the molecular genetic basis for root growth under excess Hg2＋ stress. To map quantitative trait loci （QTLs） in rice for Hg2＋ tolerance, a population of 120 recombinant inbred lines derived from a cross between two japonica cultivars Yuefu and IRAT109 was grown in 0.5 mmol/L CaCI2 solution. Relative root length （RRL）, percentage of the seminal root length in ＋HgCI2 to -HgCI2, was used for assessing Hg2＋ tolerance. In a dose-response experiment, Yuefu had a higher RRL than IRAT109 and showed the most significant difference at the Hg2＋ concentration of 1.5 tJmol/L. Three putative QTLs for RRL were detected on chromosomes 1, 2 and 5, and totally explained about 35.7% of the phenotypic variance in Hg2＋ tolerance. The identified QTLs for RRL might be useful for improving Hg2＋ tolerance of rice by molecular marker-assisted selection.展开更多
Switchgrass is an introduced C_(4)grass in the semi-arid Loess Plateau of China,but there is a lack of information to assess its ecological invasive risk.In this study,Old World bluestems(native C_(4)grass)and switchg...Switchgrass is an introduced C_(4)grass in the semi-arid Loess Plateau of China,but there is a lack of information to assess its ecological invasive risk.In this study,Old World bluestems(native C_(4)grass)and switchgrass were sowed at five mixture ratios(8:0,6:2,4:4,2:6 and 0:8)under two soil water levels(80%field capacity(FC)and 40%FC)and two nitrogen(N)treatments(0 and 100 mg N/kg dry soil,termed N0-unfertilized and N1-fertilized treatments,respectively)in a pot experiment in 2012.Biomass,root morphological traits and relative competitive abilities of these two species were analyzed.Results showed that biomass of both species was significantly greater under 80%FC or N fertilization,and switchgrass had a relatively larger root:shoot ratio(RSR).Total root length(TRL)and root surface area(RSA)of switchgrass were significantly higher under 80%FC irrespective of N treatment,while those of Old World bluestems were only significantly higher under N fertilization.N had no significant effect on TRL and RSA of switchgrass,while RSA of Old World bluestems significantly increased under 80%FC and N fertilization.Under 40%FC and N0-unfertilized treatment,the aggressivity of Old World bluestems was larger than zero at 2:6 and 4:4 mixture ratios of two species,whereas it was close to zero at 6:2 mixture ratio.Root competitive ability of switchgrass significantly increased under 80%FC or N fertilization.The aggressivity of Old World bluestems was negative at 6:2 mixture ratio under 80%FC and N fertilization,while it was positive at 2:6 mixture ratio.Switchgrass may become more aggressive when N deposition or rainfall increases,while a proper mixture ratio with appropriate water and N management could help with grassland management in the semi-arid Loess Plateau.展开更多
基金funded by the National Natural Science Foundation of China(Grant No.30771330)the National Natural Science Foundation of Zhejiang Province,China(Grant No.Z306300)the Zhejiang Normal University Innovative Research Team Program of China
文摘Mercury （Hg） is one of the most toxic heavy metals to living organisms and its conspicuous effect is the inhibition of root growth. However, little is known about the molecular genetic basis for root growth under excess Hg2＋ stress. To map quantitative trait loci （QTLs） in rice for Hg2＋ tolerance, a population of 120 recombinant inbred lines derived from a cross between two japonica cultivars Yuefu and IRAT109 was grown in 0.5 mmol/L CaCI2 solution. Relative root length （RRL）, percentage of the seminal root length in ＋HgCI2 to -HgCI2, was used for assessing Hg2＋ tolerance. In a dose-response experiment, Yuefu had a higher RRL than IRAT109 and showed the most significant difference at the Hg2＋ concentration of 1.5 tJmol/L. Three putative QTLs for RRL were detected on chromosomes 1, 2 and 5, and totally explained about 35.7% of the phenotypic variance in Hg2＋ tolerance. The identified QTLs for RRL might be useful for improving Hg2＋ tolerance of rice by molecular marker-assisted selection.
基金The study was supported by the National Natural Science Foundation of China(41371509,41771553)the Intergovernmental International Cooperation on Science and Technology Innovation under the Ministry of Science and Technology of People's Republic of China(2018YFE0112400).
文摘Switchgrass is an introduced C_(4)grass in the semi-arid Loess Plateau of China,but there is a lack of information to assess its ecological invasive risk.In this study,Old World bluestems(native C_(4)grass)and switchgrass were sowed at five mixture ratios(8:0,6:2,4:4,2:6 and 0:8)under two soil water levels(80%field capacity(FC)and 40%FC)and two nitrogen(N)treatments(0 and 100 mg N/kg dry soil,termed N0-unfertilized and N1-fertilized treatments,respectively)in a pot experiment in 2012.Biomass,root morphological traits and relative competitive abilities of these two species were analyzed.Results showed that biomass of both species was significantly greater under 80%FC or N fertilization,and switchgrass had a relatively larger root:shoot ratio(RSR).Total root length(TRL)and root surface area(RSA)of switchgrass were significantly higher under 80%FC irrespective of N treatment,while those of Old World bluestems were only significantly higher under N fertilization.N had no significant effect on TRL and RSA of switchgrass,while RSA of Old World bluestems significantly increased under 80%FC and N fertilization.Under 40%FC and N0-unfertilized treatment,the aggressivity of Old World bluestems was larger than zero at 2:6 and 4:4 mixture ratios of two species,whereas it was close to zero at 6:2 mixture ratio.Root competitive ability of switchgrass significantly increased under 80%FC or N fertilization.The aggressivity of Old World bluestems was negative at 6:2 mixture ratio under 80%FC and N fertilization,while it was positive at 2:6 mixture ratio.Switchgrass may become more aggressive when N deposition or rainfall increases,while a proper mixture ratio with appropriate water and N management could help with grassland management in the semi-arid Loess Plateau.
