Aim To discover the oxygenated monoterpenes that play a critical role in the biosynthetic pathway of monoterpene in Rosa damascene.Method Several kinds of isolation techniques such as liquid-liquid partition,silica co...Aim To discover the oxygenated monoterpenes that play a critical role in the biosynthetic pathway of monoterpene in Rosa damascene.Method Several kinds of isolation techniques such as liquid-liquid partition,silica column chromatography,thin layer chromatography and high performance liquid chromatography were used to separate the compounds and several identification techniques such as GC-MS and 1H-NMR were used to determine the structure of the isolated compounds.Results Three oxidized acyclic monoterpenes were obtained.They are 8-hydroxygeraniol,8-hydroxynerol and 8-hydroxylinalool.Conclusion The compounds are the important materials of pharmaceutical,cosmetic and food industries.The work has not only provided a new method for the determination of the main aromatic components of Rosa damascena,but also built up an important foundation for the study of biosynthesis of monoterpenoids in the plant.展开更多
保加利亚大马士革玫瑰属蔷薇科蔷薇属玫瑰中的一种富含玫瑰油的品种,采用传统扦插方法进行繁殖,但是从加拿大引入的该种生根率极低。为提高其生根率,试验采用4个浓度的生根粉和3种基质对3个不同品种(fourseasons、celsiana、rose de res...保加利亚大马士革玫瑰属蔷薇科蔷薇属玫瑰中的一种富含玫瑰油的品种,采用传统扦插方法进行繁殖,但是从加拿大引入的该种生根率极低。为提高其生根率,试验采用4个浓度的生根粉和3种基质对3个不同品种(fourseasons、celsiana、rose de rescht)进行扦插,同时控制其温度与湿度,结果表明:3个品种的扦插成活率品种1高,品种2最低,其基质的选择为珍珠岩最好,其次是草炭和珍珠岩混合,由于草炭透气性差,故成活率较低,3个品种在生根粉浓度的选择上依次选用50mg·kg-1、100mg·kg-1、30mg·kg-1。展开更多
Objective:To make full usage of resource and turn waste into treasure,the chemical constituents and bioactivity were firstly investigated on Damask rose(Rosa damascena)flower residue(DRFR).Methods:DPPH and ABTS experi...Objective:To make full usage of resource and turn waste into treasure,the chemical constituents and bioactivity were firstly investigated on Damask rose(Rosa damascena)flower residue(DRFR).Methods:DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR.Then,column chromatography was used to purify compounds from an antioxidation extract(DRFR-A),and the chemical structure was identified using NMR.The total phenolic acid content was measured by FolinCiocalteu colorimetric method,and the content of gallic acid of the indicator ingredient was detected by HPLC.Results:DRFR-A was found to show a high activity both on DPPH(IC5o:2.760μg/mL)and ABTS(ICso:2.258μg/mL)compared to positive control Vc,Ten compounds were isolated and identified as quercetin(1),kaempferol(2),gallic acid(3),protocatechuic acid(4),pyrogallic acid(5),2-phenylethyl 3,4,5-trihydroxybenzoate(6),methyl gallate(7),p-hydroxybenzoic acid(8),p-hydroxyphenethyl alcohol(9)and astragalin(10)from DRFR-A.Among them,pyrogallic acid,2-phenylethyl-3,4,5-trihydroxybenzoate,p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time.The content of total phenolic acids and gallic acid,main ingredient in DRFR-A was determined as 63.73%and 24.67%,respectively.Conclusion:This study provides a reliable data and lays the foundation for the development and utilization of rose residue,and hence for the full utilization of rose resources.展开更多
Background:Constipation is a common chronic bowel disorder with an incidence of more than 50%in the elderly population.Complementary and alternative medicine is a cost-effective and satisfactory treatment for constipa...Background:Constipation is a common chronic bowel disorder with an incidence of more than 50%in the elderly population.Complementary and alternative medicine is a cost-effective and satisfactory treatment for constipation used widely by the elderly.Objective:This study evaluates the efficacy of an herbal formula made from Foeniculum vulgare Mill.and Rosa damascena for the treatment of constipation in an elderly population and consequent changes to their quality of life.Design,setting,participants and intervention:This double-blind randomized active controlled clinical trial,with parallel group allocation ratio of 1:1,was conducted in a referral clinic in Afzalipour Hospital,affiliated to Kerman University of Medical Sciences in Kerman,Southeastern Iran.Individuals over 60 years of age,diagnosed with functional constipation(based on the RomeⅣcriteria),were included in this study.Participants received a sachet of 10 g F.vulgare and R.damascena(herbal formula group)or polyethylene glycol 4000(PEG 4000 group)with a glass of warm water two times a day for 4 weeks and were followed up for 4 additional weeks.Main outcome measures:Constipation severity,stool consistency,and the quality of life were used as the primary outcomes.Drug side effects were used as a secondary outcome.The outcomes were assessed using the Constipation Assessment Scale,the Bristol Stool Form Scale,and the Patient Assessment of Constipation Quality of Life questionnaire.Results:A total of 25 participants in each group completed the four-week treatment cycle and the eightweek follow-up.At the end of the four-week treatment cycle,all clinical outcomes had significant improvements in both groups(P<0.05).The analysis of constipation severity(P<0.001),stool consistency(P<0.001),and the quality of life(P<0.001)showed significant improvements with fewer side effects(mild diarrhea)and a longer duration of symptom relief in the herbal formula group compared to the PEG 4000 group.Conclusion:Although both interventions significantly improved the treatment outcomes,constipation severity,stool consistency and the quality of life were improved more effectively by the herbal formula than by PEG 4000;however,the mechanism of action is not yet understood.Trial registration:This trial was registered in the Iranian Registry of Clinical Trials(IRCTID:IRCT20200108046056N1).展开更多
文摘Aim To discover the oxygenated monoterpenes that play a critical role in the biosynthetic pathway of monoterpene in Rosa damascene.Method Several kinds of isolation techniques such as liquid-liquid partition,silica column chromatography,thin layer chromatography and high performance liquid chromatography were used to separate the compounds and several identification techniques such as GC-MS and 1H-NMR were used to determine the structure of the isolated compounds.Results Three oxidized acyclic monoterpenes were obtained.They are 8-hydroxygeraniol,8-hydroxynerol and 8-hydroxylinalool.Conclusion The compounds are the important materials of pharmaceutical,cosmetic and food industries.The work has not only provided a new method for the determination of the main aromatic components of Rosa damascena,but also built up an important foundation for the study of biosynthesis of monoterpenoids in the plant.
文摘保加利亚大马士革玫瑰属蔷薇科蔷薇属玫瑰中的一种富含玫瑰油的品种,采用传统扦插方法进行繁殖,但是从加拿大引入的该种生根率极低。为提高其生根率,试验采用4个浓度的生根粉和3种基质对3个不同品种(fourseasons、celsiana、rose de rescht)进行扦插,同时控制其温度与湿度,结果表明:3个品种的扦插成活率品种1高,品种2最低,其基质的选择为珍珠岩最好,其次是草炭和珍珠岩混合,由于草炭透气性差,故成活率较低,3个品种在生根粉浓度的选择上依次选用50mg·kg-1、100mg·kg-1、30mg·kg-1。
基金The authors wish to thank associate professor Cheng-xue Pan from Zheng-zhou University for his assistance in the plants identification and authentications.This project was supported financially by the National Natural Science Foundation of China(Grant No.331/313400531021).
文摘Objective:To make full usage of resource and turn waste into treasure,the chemical constituents and bioactivity were firstly investigated on Damask rose(Rosa damascena)flower residue(DRFR).Methods:DPPH and ABTS experiments were applied to assess the antioxidant activity of DRFR.Then,column chromatography was used to purify compounds from an antioxidation extract(DRFR-A),and the chemical structure was identified using NMR.The total phenolic acid content was measured by FolinCiocalteu colorimetric method,and the content of gallic acid of the indicator ingredient was detected by HPLC.Results:DRFR-A was found to show a high activity both on DPPH(IC5o:2.760μg/mL)and ABTS(ICso:2.258μg/mL)compared to positive control Vc,Ten compounds were isolated and identified as quercetin(1),kaempferol(2),gallic acid(3),protocatechuic acid(4),pyrogallic acid(5),2-phenylethyl 3,4,5-trihydroxybenzoate(6),methyl gallate(7),p-hydroxybenzoic acid(8),p-hydroxyphenethyl alcohol(9)and astragalin(10)from DRFR-A.Among them,pyrogallic acid,2-phenylethyl-3,4,5-trihydroxybenzoate,p-hydroxybenzoic acid and p-hydroxyphenethyl alcohol are obtained from the plant for the first time.The content of total phenolic acids and gallic acid,main ingredient in DRFR-A was determined as 63.73%and 24.67%,respectively.Conclusion:This study provides a reliable data and lays the foundation for the development and utilization of rose residue,and hence for the full utilization of rose resources.
基金the authorities at the Kerman University of Medical Sciences for supporting this study。
文摘Background:Constipation is a common chronic bowel disorder with an incidence of more than 50%in the elderly population.Complementary and alternative medicine is a cost-effective and satisfactory treatment for constipation used widely by the elderly.Objective:This study evaluates the efficacy of an herbal formula made from Foeniculum vulgare Mill.and Rosa damascena for the treatment of constipation in an elderly population and consequent changes to their quality of life.Design,setting,participants and intervention:This double-blind randomized active controlled clinical trial,with parallel group allocation ratio of 1:1,was conducted in a referral clinic in Afzalipour Hospital,affiliated to Kerman University of Medical Sciences in Kerman,Southeastern Iran.Individuals over 60 years of age,diagnosed with functional constipation(based on the RomeⅣcriteria),were included in this study.Participants received a sachet of 10 g F.vulgare and R.damascena(herbal formula group)or polyethylene glycol 4000(PEG 4000 group)with a glass of warm water two times a day for 4 weeks and were followed up for 4 additional weeks.Main outcome measures:Constipation severity,stool consistency,and the quality of life were used as the primary outcomes.Drug side effects were used as a secondary outcome.The outcomes were assessed using the Constipation Assessment Scale,the Bristol Stool Form Scale,and the Patient Assessment of Constipation Quality of Life questionnaire.Results:A total of 25 participants in each group completed the four-week treatment cycle and the eightweek follow-up.At the end of the four-week treatment cycle,all clinical outcomes had significant improvements in both groups(P<0.05).The analysis of constipation severity(P<0.001),stool consistency(P<0.001),and the quality of life(P<0.001)showed significant improvements with fewer side effects(mild diarrhea)and a longer duration of symptom relief in the herbal formula group compared to the PEG 4000 group.Conclusion:Although both interventions significantly improved the treatment outcomes,constipation severity,stool consistency and the quality of life were improved more effectively by the herbal formula than by PEG 4000;however,the mechanism of action is not yet understood.Trial registration:This trial was registered in the Iranian Registry of Clinical Trials(IRCTID:IRCT20200108046056N1).
