Background:Rosa chinensis Jacq.and Rosa rugosa Thunb.are not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional medicines.The two plants h...Background:Rosa chinensis Jacq.and Rosa rugosa Thunb.are not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional medicines.The two plants have some differences in efficacy,but the flower buds are easily confused for similar traits.In addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal roses.Therefore,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography fingerprints.Then,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large differences.Differential compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and Meigui.Results:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the samples.Through principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four groups.Partial least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavonoid glycosides,as well as phenolic acids.Eight differential components were screened out by orthogonal partial least-squares discriminant analysis and S-plot analysis.Quantitative analyses of the eight compounds,including gallic acid,ellagic acid,hyperoside,isoquercitrin,etc.,showed that Yueji was generally richer in phenolic acids and flavonoids than Meigui,and the quality of Yueji from Shandong and Hebei was better.It is worth noting that Xinjiang rose is rich in various components,which is worth focusing on more in-depth research.Conclusion:In this study,the fingerprints of Meigui and Yueji were established.The chemical components information of roses was further improved based on non-targeted metabolomics and mass spectrometry technology.At the same time,eight differential components of Meigui and Yueji were screened out and quantitatively analyzed.The research results provided a scientific basis for the quality control and rational development and utilization of Rosae Chinensis Flos and Rosae Rugosae Flos,and also laid a foundation for the study of their pharmacodynamic material basis.展开更多
[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in S...[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples collected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en- dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re- sistant strains which were all derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ- ment-friendly fungal biological control.展开更多
This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification ...This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column.GC,FT-IR and NMR analyses revealed that RCJ2-Ib(3.3 k Da)was a 1,4-linked polymannuronic acid containing substantialβ-Danomers units.The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1.In addition,RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli.As a result,RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.展开更多
The tissue cullurc of Schloss Mannheim (Rosa Chinensus , var. Florihunda ) with full and unsprouting bud of stem segments as the explants was experimented The result shows that the buds sprouted best on MS med...The tissue cullurc of Schloss Mannheim (Rosa Chinensus , var. Florihunda ) with full and unsprouting bud of stem segments as the explants was experimented The result shows that the buds sprouted best on MS medium with the addition of 6-HA 1 .0 mg/L,. and differentiation was best on MS medium with addition of 6-BA 1 .5 + NAA0.05 + ZT0.1 mg/L, or KT 1,0 + NAA0,05 + ZT0.1mg/L. The MS emdium with addition of 6-BA 0.3 + NAA 0.0 5+ ZT0.1 mg/L, or KT 0.3 + NAA0.05 +ZT0. 1 mg/L showed a good result for developing strong shoots. 1/2 MS medium with the addition ofIBA 0.1 mg/L, or IBA0.1 + NAA0.02 mg/L, had best result for rooting. The plantlets should be transplanted from test-tube to soil when they grew ' to 2.5 ~ 4.0 cm high and have 3 ~ 5 strips short roots. Ahigher survival rate was obtained under the conditions of conterolling humidity and temperature展开更多
According to the hazards and occurrence characteristics of major diseases of Rosa chinensis, such as powdery mildew, branch canker and black spot, and major pests such as mites, aphids, thrips and Lepidoptera, prevent...According to the hazards and occurrence characteristics of major diseases of Rosa chinensis, such as powdery mildew, branch canker and black spot, and major pests such as mites, aphids, thrips and Lepidoptera, prevention and control of the diseases and pests should be based on pruning, garden cleaning, water and fertilizer management, and chemical control can be applied as the supplement. Suitable prevention and control measures should be adopted for preventing the disea- ses and pests scientifically.展开更多
Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the gene...Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the genetic determinism of perpetual blooming, we developed six rose populations(OB, W, F1, F2, BC1 OB and BC1W) derived from a WOB population [interspecific diploid hybridization between Rosa chinensis ‘Old Blush'(OB) and R. wichuriana ‘Basye's Thornless'(W)]. Perpetual blooming is absent both in a F1 population with 296 individuals and a BC1 W population(W as the backcross parent) with 150 individuals. However, the perpetual blooming trait showed a typical 3︰1 segregation in a backcross population BC1 OB with OB as the backcross parent. In this population with 300 individuals, 83 plants had the perpetual blooming phenotype while the other 217 featured non-perpetual blooming, indicating that the perpetual blooming trait is very likely controlled by two recessive genes in R. chinensis(rpb1 and rpb2). These genetic data suggest that the inheritance of rose perpetual blooming may be controlled by a complex mechanism.展开更多
Rosa chinensis as edible flowers,is a kind of traditional Chinese medicine,which is rich in resources and is cultivated in most parts of China.Its phytochemicals mainly include flavonoids and their glycosides,phenolic...Rosa chinensis as edible flowers,is a kind of traditional Chinese medicine,which is rich in resources and is cultivated in most parts of China.Its phytochemicals mainly include flavonoids and their glycosides,phenolic acids,pentacyclic triterpenes and steroids.R.chinensis has many biological activities,including antioxidation,antibiosis,protection of pancreatic islet cells.The phytochemicals and biological activities of R.chinensis were summarized by databases such as Web of Science,SciFinder,Baidu Scholar,PubMed,ScienceDirect and SpringerLink.展开更多
基金supported by the key project at the central government level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant number 2060302)the National Natural Science Foundation of China(Grant number 82373982,82173929).
文摘Background:Rosa chinensis Jacq.and Rosa rugosa Thunb.are not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional medicines.The two plants have some differences in efficacy,but the flower buds are easily confused for similar traits.In addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal roses.Therefore,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography fingerprints.Then,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large differences.Differential compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and Meigui.Results:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the samples.Through principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four groups.Partial least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavonoid glycosides,as well as phenolic acids.Eight differential components were screened out by orthogonal partial least-squares discriminant analysis and S-plot analysis.Quantitative analyses of the eight compounds,including gallic acid,ellagic acid,hyperoside,isoquercitrin,etc.,showed that Yueji was generally richer in phenolic acids and flavonoids than Meigui,and the quality of Yueji from Shandong and Hebei was better.It is worth noting that Xinjiang rose is rich in various components,which is worth focusing on more in-depth research.Conclusion:In this study,the fingerprints of Meigui and Yueji were established.The chemical components information of roses was further improved based on non-targeted metabolomics and mass spectrometry technology.At the same time,eight differential components of Meigui and Yueji were screened out and quantitatively analyzed.The research results provided a scientific basis for the quality control and rational development and utilization of Rosae Chinensis Flos and Rosae Rugosae Flos,and also laid a foundation for the study of their pharmacodynamic material basis.
基金Supported by National Natural Science Foundation of China(No.31000144)Project of Shaanxi Provincial Science and Technology Department(No.2012JQ3017)+1 种基金Project of Xi'an Science and Technology Bureau[NC1206(3)]Project of Education Department of Shaanxi Provincial Government(No.12JK1106)~~
文摘[Objective] This study aimed to isolate and screen black-spot-resistant en- dophytic fungi strains. [Method] Various species of healthy Rosa chinensis were col- lected from Xi＇an, Xianyang, Baoji and Weinan City in Shaanxi Province. Endophytic fungi was isolated from stems and leaves, and purified by 2-3 times of inoculation to screen endophytic fungi antagonistic to Marssonina rosae with modified punching method. [Result] Samples collected from Xianyang exhibited the highest colonization rate and isolation rate; endophytic fungi strains isolated from stems presented the highest colonization rate and isolation rate compared with leaves. A total of 67 en- dophytic fungi strains were isolated from Rosa chinensis, including 3 black spot-re- sistant strains which were all derived from Baoji. [Conclusion] This study laid the foundation for further screening candidate strains of biocontrol fungi and environ- ment-friendly fungal biological control.
基金supported by Research on Precision Nutrition and Health Food,Department of Science and Technology of Henan Province(CXJD2021006)Key Project in Science and Technology Agency of Henan Province(202102110283 and 202102110149)。
文摘This is the first report on a polysaccharide(RCJ2-Ib)isolated from Rosa Chinensis Flos.RCJ2-Ib was obtained through the extraction with water,precipitation with ethanol,separation with DEAE-52 column and purification with DEAE-Sepharose Fast Flow column and Sephadex G100 column.GC,FT-IR and NMR analyses revealed that RCJ2-Ib(3.3 k Da)was a 1,4-linked polymannuronic acid containing substantialβ-Danomers units.The anticoagulant effect of RCJ2-Ib evaluated by using rabbit ear venous blood and an acute blood stasis rat model showed that RCJ2-Ib had obvious anticoagulant activity in regulating endogenous and exogenous coagulation pathways and reducing serum thromboxane B2 and endothelin-1.In addition,RCJ2-Ib could also increase the number of Lactobacillus and Escherichia coli.As a result,RCJ2-Ib has the potential to inhibit thrombosis and maintain the intestinal environment.
文摘The tissue cullurc of Schloss Mannheim (Rosa Chinensus , var. Florihunda ) with full and unsprouting bud of stem segments as the explants was experimented The result shows that the buds sprouted best on MS medium with the addition of 6-HA 1 .0 mg/L,. and differentiation was best on MS medium with addition of 6-BA 1 .5 + NAA0.05 + ZT0.1 mg/L, or KT 1,0 + NAA0,05 + ZT0.1mg/L. The MS emdium with addition of 6-BA 0.3 + NAA 0.0 5+ ZT0.1 mg/L, or KT 0.3 + NAA0.05 +ZT0. 1 mg/L showed a good result for developing strong shoots. 1/2 MS medium with the addition ofIBA 0.1 mg/L, or IBA0.1 + NAA0.02 mg/L, had best result for rooting. The plantlets should be transplanted from test-tube to soil when they grew ' to 2.5 ~ 4.0 cm high and have 3 ~ 5 strips short roots. Ahigher survival rate was obtained under the conditions of conterolling humidity and temperature
基金Sponsored by National Spark Program(2012GA800003-6)Key Scientific and Technological Program of Hainan Province(ZDZXZ013012)Scientific and Technological Fruits Transformation Project of Sanya(2013CZ09)
文摘According to the hazards and occurrence characteristics of major diseases of Rosa chinensis, such as powdery mildew, branch canker and black spot, and major pests such as mites, aphids, thrips and Lepidoptera, prevention and control of the diseases and pests should be based on pruning, garden cleaning, water and fertilizer management, and chemical control can be applied as the supplement. Suitable prevention and control measures should be adopted for preventing the disea- ses and pests scientifically.
基金funded by a grant from the National Natural Science Foundation of China(31160402)
文摘Perpetual blooming is one of the most important biological and economical traits in modern rose, while the genetic basis underlining the control of this trait is poorly investigated. With an aim in dissecting the genetic determinism of perpetual blooming, we developed six rose populations(OB, W, F1, F2, BC1 OB and BC1W) derived from a WOB population [interspecific diploid hybridization between Rosa chinensis ‘Old Blush'(OB) and R. wichuriana ‘Basye's Thornless'(W)]. Perpetual blooming is absent both in a F1 population with 296 individuals and a BC1 W population(W as the backcross parent) with 150 individuals. However, the perpetual blooming trait showed a typical 3︰1 segregation in a backcross population BC1 OB with OB as the backcross parent. In this population with 300 individuals, 83 plants had the perpetual blooming phenotype while the other 217 featured non-perpetual blooming, indicating that the perpetual blooming trait is very likely controlled by two recessive genes in R. chinensis(rpb1 and rpb2). These genetic data suggest that the inheritance of rose perpetual blooming may be controlled by a complex mechanism.
基金supported by Kaifeng Science and Technology Bureau Project(1908007).
文摘Rosa chinensis as edible flowers,is a kind of traditional Chinese medicine,which is rich in resources and is cultivated in most parts of China.Its phytochemicals mainly include flavonoids and their glycosides,phenolic acids,pentacyclic triterpenes and steroids.R.chinensis has many biological activities,including antioxidation,antibiosis,protection of pancreatic islet cells.The phytochemicals and biological activities of R.chinensis were summarized by databases such as Web of Science,SciFinder,Baidu Scholar,PubMed,ScienceDirect and SpringerLink.
