This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced u...This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Compared to control group,DSS induction decreased body weight of mice,indexes of Shannon,Simpson,Chao1 and Faith_pd,and increased disease activity index(DAI)and levels of interleukin 1β(IL-1β),IL-6,tumor necrosis factorα(TNF-α)and interferon-γ(IFN-γ);And this induction also led to an increase in Proteobacteria,Verrucomicrobia and Actinobacteria at phylum level,harmful bacterial species richness at genus level,and relative richness of S.sciuri,Desulfovibrio C21_c20,R.gnavus and Akkermansia muciniphila at species level,and a decrease in Firmicutes at phylum level and relative richness of B.acidifaciens in mice.LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis(UC)and indexes of Shannon,Simpson,Chao1 and Faith_pd,reduced DAI and the content of four infl ammatory factors and improved gut microbiota imbalance in DSS induced UC mice.Besides,the number of operational taxonomic units(OTUs)increased,α-diversity andβ-diversity were restored and similar to those in mice in the control group after LAB-RRTJ treatment.Compared with the positive drug treatment group,LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice.Correlation analysis showed that infl ammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice.Taken together,our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of infl ammatory cytokines and gut microbiota composition.展开更多
The fruit of cultivar Guinong5 (Rosa roxburghii Tratt.) was used to investigate the regularity of its growth and development, and the changes of endogenous hormone contents during the period of the growth and developm...The fruit of cultivar Guinong5 (Rosa roxburghii Tratt.) was used to investigate the regularity of its growth and development, and the changes of endogenous hormone contents during the period of the growth and development. The result showed that contents of GA 1+3, IAA and ZRs in the flower receptacle of young fruit were high and then decreased, contents of GA 1+3and IAA in seeds increased with its development until maturity. The contents of ZRs and ABA in the flower receptacle of young fruits and seeds were high, and then had a tendency of descent with the development of fruits. The contents of GA 1+3, IAA, ZRs and ABA in flower receptacle of young fruit with normal fertilization were much higher than those of in non-fertilization fruits. In the flower receptacle of malformed fruits, the contents of GA 1+3, IAA and ZRs in normally developing flower receptacle were significantly higher than those in malformed fruits. The growth and development of fruits depended largely on the seeds, development because the seeds in the fruits with non-fertilization failed to be formed or even formed but irreproducible, which would lead to the decrease of GA 1+3, IAA contents and the abnormal development of fruits. Therefore the fruits deformed and dropped inevitably.展开更多
该文采用超高效液相色谱-高分辨质谱技术(ultra high performance liquid chromatography high resolution mass spectrometry,UPLC-HRMS)对刺梨不同提取物进行非靶向定性解析,通过数据库确认注释并鉴定代谢物,在二级质谱信息下共鉴定出...该文采用超高效液相色谱-高分辨质谱技术(ultra high performance liquid chromatography high resolution mass spectrometry,UPLC-HRMS)对刺梨不同提取物进行非靶向定性解析,通过数据库确认注释并鉴定代谢物,在二级质谱信息下共鉴定出316种代谢物。运用多元统计方法进行刺梨不同提取物数据分析并筛选出差异代谢物。主成分分析结果表明,刺梨不同提取物存在代谢物差异。依据正交偏最小二乘法判别分析模型的变量重要性投影(variable importance projection value,VIP,VIP>1.3)值、P<0.01和差异倍数(fold change,FC,FC≥3和FC≤0.1)可筛选出49种显著差异代谢物。通过MBRole 2.0通路分析功能,发现有11条代谢途径。其中类黄酮生物合成途径最为显著,共计4个代谢物参与类黄酮生物合成代谢通路。该文基于非靶向代谢组学分析表明,刺梨不同提取物之间的代谢物有一定差异,为进一步研究其功能提供了数据支持。展开更多
基金supported by Rosa roxburghii industry development program of Guizhou Province,China(QCN2019-261)the National Natural Science Foundation of China(31260379)the National Natural Science Foundation of China(31960485).
文摘This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Compared to control group,DSS induction decreased body weight of mice,indexes of Shannon,Simpson,Chao1 and Faith_pd,and increased disease activity index(DAI)and levels of interleukin 1β(IL-1β),IL-6,tumor necrosis factorα(TNF-α)and interferon-γ(IFN-γ);And this induction also led to an increase in Proteobacteria,Verrucomicrobia and Actinobacteria at phylum level,harmful bacterial species richness at genus level,and relative richness of S.sciuri,Desulfovibrio C21_c20,R.gnavus and Akkermansia muciniphila at species level,and a decrease in Firmicutes at phylum level and relative richness of B.acidifaciens in mice.LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis(UC)and indexes of Shannon,Simpson,Chao1 and Faith_pd,reduced DAI and the content of four infl ammatory factors and improved gut microbiota imbalance in DSS induced UC mice.Besides,the number of operational taxonomic units(OTUs)increased,α-diversity andβ-diversity were restored and similar to those in mice in the control group after LAB-RRTJ treatment.Compared with the positive drug treatment group,LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice.Correlation analysis showed that infl ammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice.Taken together,our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of infl ammatory cytokines and gut microbiota composition.
文摘The fruit of cultivar Guinong5 (Rosa roxburghii Tratt.) was used to investigate the regularity of its growth and development, and the changes of endogenous hormone contents during the period of the growth and development. The result showed that contents of GA 1+3, IAA and ZRs in the flower receptacle of young fruit were high and then decreased, contents of GA 1+3and IAA in seeds increased with its development until maturity. The contents of ZRs and ABA in the flower receptacle of young fruits and seeds were high, and then had a tendency of descent with the development of fruits. The contents of GA 1+3, IAA, ZRs and ABA in flower receptacle of young fruit with normal fertilization were much higher than those of in non-fertilization fruits. In the flower receptacle of malformed fruits, the contents of GA 1+3, IAA and ZRs in normally developing flower receptacle were significantly higher than those in malformed fruits. The growth and development of fruits depended largely on the seeds, development because the seeds in the fruits with non-fertilization failed to be formed or even formed but irreproducible, which would lead to the decrease of GA 1+3, IAA contents and the abnormal development of fruits. Therefore the fruits deformed and dropped inevitably.
文摘该文采用超高效液相色谱-高分辨质谱技术(ultra high performance liquid chromatography high resolution mass spectrometry,UPLC-HRMS)对刺梨不同提取物进行非靶向定性解析,通过数据库确认注释并鉴定代谢物,在二级质谱信息下共鉴定出316种代谢物。运用多元统计方法进行刺梨不同提取物数据分析并筛选出差异代谢物。主成分分析结果表明,刺梨不同提取物存在代谢物差异。依据正交偏最小二乘法判别分析模型的变量重要性投影(variable importance projection value,VIP,VIP>1.3)值、P<0.01和差异倍数(fold change,FC,FC≥3和FC≤0.1)可筛选出49种显著差异代谢物。通过MBRole 2.0通路分析功能,发现有11条代谢途径。其中类黄酮生物合成途径最为显著,共计4个代谢物参与类黄酮生物合成代谢通路。该文基于非靶向代谢组学分析表明,刺梨不同提取物之间的代谢物有一定差异,为进一步研究其功能提供了数据支持。
